6903751

(5E)-1-(2-fluorophenyl)-5-(1H-pyrrol-2-ylmethylidene)-1,3-diazinane-2,4,6-trione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2223
Screen concentration 107.6 μM
Source Chembridge (Fragment library)
PubChem CID 5598863
SMILES C1=CC=C(C(=C1)N2C(=O)C(=CC3=CC=CN3)C(=O)NC2=O)F
Standardized SMILES Fc1ccccc1N2C(=O)NC(=O)C(=Cc3ccc[nH]3)C2=O
Molecular weight 299.2566
ALogP 2.24
H-bond donor count 2
H-bond acceptor count 4
Response signature superoxide

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.39
% growth inhibition (Hom. pool) 7.6


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5598863
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:3.96|P-value:3.68E-5|Clearance:0.17||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ARP3(YJR065C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ARP9(YMR033W)|FD-Score:3.19|P-value:6.99E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT6(YDR188W)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DIM1(YPL266W)|FD-Score:4.79|P-value:8.16E-7|Clearance:0.64||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:ERG10(YPL028W)|FD-Score:3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:HEM12(YDR047W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.15||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MIA40(YKL195W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.02||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOP56(YLR197W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.64||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:5.44|P-value:2.61E-8|Clearance:0.64||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.04||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RLI1(YDR091C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC53(YDL150W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC6(YCR052W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SUP35(YDR172W)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.03||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TIF35(YDR429C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM22(YDL217C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TOM40(YMR203W)|FD-Score:4.88|P-value:5.25E-7|Clearance:0.64||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YLR198C(YLR198C_d)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YTH1(YPR107C)|FD-Score:3.75|P-value:8.69E-5|Clearance:0.36||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ALG14(YBR070C)|FD-Score:3.96|P-value:3.68E-5|Clearance:0.17||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:ARP3(YJR065C)|FD-Score:3.13|P-value:8.89E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ARP9(YMR033W)|FD-Score:3.19|P-value:6.99E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CCT6(YDR188W)|FD-Score:-3.61|P-value:1.50E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:DIM1(YPL266W)|FD-Score:4.79|P-value:8.16E-7|Clearance:0.64||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:ERG10(YPL028W)|FD-Score:3.11|P-value:9.41E-4|Clearance:0||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:HEM12(YDR047W)|FD-Score:3.35|P-value:4.09E-4|Clearance:0.15||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:MIA40(YKL195W)|FD-Score:3.15|P-value:8.29E-4|Clearance:0.02||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:NOP56(YLR197W)|FD-Score:-4.08|P-value:2.28E-5|Clearance:0||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:NOP8(YOL144W)|FD-Score:5.04|P-value:2.31E-7|Clearance:0.64||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:PKC1(YBL105C)|FD-Score:5.44|P-value:2.61E-8|Clearance:0.64||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIM2(YBR192W)|FD-Score:3.79|P-value:7.52E-5|Clearance:0.04||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RLI1(YDR091C)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.02||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPC53(YDL150W)|FD-Score:-3.51|P-value:2.24E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RSC6(YCR052W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:4.16|P-value:1.60E-5|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SUP35(YDR172W)|FD-Score:3.38|P-value:3.61E-4|Clearance:0.03||SGD DESC:Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype Gene:TIF35(YDR429C)|FD-Score:-3.56|P-value:1.84E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM22(YDL217C)|FD-Score:3.1|P-value:9.52E-4|Clearance:0.06||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TOM40(YMR203W)|FD-Score:4.88|P-value:5.25E-7|Clearance:0.64||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YLR198C(YLR198C_d)|FD-Score:-3.15|P-value:8.10E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YTH1(YPR107C)|FD-Score:3.75|P-value:8.69E-5|Clearance:0.36||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5598863
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADH3(YMR083W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ARC18(YLR370C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP1(YHR129C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:COG7(YGL005C)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWC27(YPL064C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:CYT2(YKL087C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:FYV6(YNL133C)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCS1(YDL226C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GID8(YMR135C)|FD-Score:4.3|P-value:8.52E-6||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GND1(YHR183W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-4.77|P-value:9.20E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL31(YBR298C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MDH1(YKL085W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MEU1(YLR017W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MTC7(YEL033W_p)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NPT1(YOR209C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OPI9(YLR338W_d)|FD-Score:-4.87|P-value:5.52E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU17(YLL025W)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PER1(YCR044C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX15(YOL044W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PEX3(YDR329C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:RGP1(YDR137W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPE1(YJL121C)|FD-Score:6.33|P-value:1.22E-10||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.61|P-value:1.98E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SAC6(YDR129C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC22(YLR268W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHP1(YBL058W)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLA2(YNL243W)|FD-Score:-4.27|P-value:9.60E-6||SGD DESC:Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo Gene:SPO1(YNL012W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:STV1(YMR054W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SWI4(YER111C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDA9(YML081W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:9.67|P-value:2.03E-22||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TUB3(YML124C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:14.4|P-value:3.28E-47||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.49|P-value:2.01E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.78|P-value:7.97E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBL083C(YBL083C_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR292C(YBR292C_d)|FD-Score:5.73|P-value:4.89E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YDR271C(YDR271C_d)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YGR164W(YGR164W_d)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL120W(YJL120W_d)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR280C(YLR280C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ACO1(YLR304C)|FD-Score:-3.86|P-value:5.68E-5||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADH3(YMR083W)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production Gene:ARC18(YLR370C)|FD-Score:-4.1|P-value:2.10E-5||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP1(YHR129C)|FD-Score:3.82|P-value:6.79E-5||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:COG7(YGL005C)|FD-Score:-4.33|P-value:7.57E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CWC27(YPL064C)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:CYT2(YKL087C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:FYV6(YNL133C)|FD-Score:-3.93|P-value:4.32E-5||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GCS1(YDL226C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GID8(YMR135C)|FD-Score:4.3|P-value:8.52E-6||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GND1(YHR183W)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication Gene:IRS4(YKR019C)|FD-Score:-4.77|P-value:9.20E-7||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL31(YBR298C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MDH1(YKL085W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Mitochondrial malate dehydrogenase, catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated Gene:MEU1(YLR017W)|FD-Score:-3.8|P-value:7.11E-5||SGD DESC:Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Gene:MTC7(YEL033W_p)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:NPT1(YOR209C)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OPI9(YLR338W_d)|FD-Score:-4.87|P-value:5.52E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:PAU17(YLL025W)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Protein of unknown function, member of the seripauperin multigene family encoded mainly in subtelomeric regions; YLL025W is not an essential gene Gene:PER1(YCR044C)|FD-Score:-3.29|P-value:4.95E-4||SGD DESC:Protein of the endoplasmic reticulum, required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1 Gene:PEX15(YOL044W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Phosphorylated tail-anchored type II integral peroxisomal membrane protein required for peroxisome biogenesis, cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol, overexpression results in impaired peroxisome assembly Gene:PEX3(YDR329C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Peroxisomal membrane protein (PMP) required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p Gene:RGP1(YDR137W)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPE1(YJL121C)|FD-Score:6.33|P-value:1.22E-10||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:3.86|P-value:5.69E-5||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-4.61|P-value:1.98E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPP2A(YOL039W)|FD-Score:-3.14|P-value:8.42E-4||SGD DESC:Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm Gene:SAC6(YDR129C)|FD-Score:-3.27|P-value:5.35E-4||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SEC22(YLR268W)|FD-Score:4.59|P-value:2.26E-6||SGD DESC:R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog Gene:SHP1(YBL058W)|FD-Score:-3.4|P-value:3.32E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SLA2(YNL243W)|FD-Score:-4.27|P-value:9.60E-6||SGD DESC:Adaptor protein that links actin to clathrin and endocytosis; involved in membrane cytoskeleton assembly and cell polarization; present in the actin cortical patch of the emerging bud tip; dimer in vivo Gene:SPO1(YNL012W)|FD-Score:3.93|P-value:4.28E-5||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:STV1(YMR054W)|FD-Score:3.29|P-value:5.09E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:SWI4(YER111C)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDA9(YML081W)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:9.67|P-value:2.03E-22||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:-3.91|P-value:4.67E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM7(YNL070W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TUB3(YML124C)|FD-Score:-3.31|P-value:4.66E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:14.4|P-value:3.28E-47||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VPS45(YGL095C)|FD-Score:-5.49|P-value:2.01E-8||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS51(YKR020W)|FD-Score:-3.78|P-value:7.97E-5||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p Gene:YBL083C(YBL083C_d)|FD-Score:3.18|P-value:7.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YBR292C(YBR292C_d)|FD-Score:5.73|P-value:4.89E-9||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene Gene:YDR271C(YDR271C_d)|FD-Score:-3.54|P-value:2.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YGR164W(YGR164W_d)|FD-Score:4.35|P-value:6.93E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL029C(YIL029C_p)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YJL120W(YJL120W_d)|FD-Score:5.08|P-value:1.84E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YLR280C(YLR280C_d)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL058C(YNL058C_p)|FD-Score:5.87|P-value:2.19E-9||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YPT35(YHR105W)|FD-Score:3.2|P-value:6.97E-4||SGD DESC:Endosomal protein of unknown function that contains a phox (PX) homology domain and binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport Gene:YPT6(YLR262C)|FD-Score:-3.31|P-value:4.67E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBL105C5.442.61E-80.64PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YOL144W5.042.31E-70.64NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YMR203W4.885.25E-70.64TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YPL266W4.798.16E-70.64DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YFR037C4.161.60E-50.17RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YCR052W3.983.39E-50.02RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YBR070C3.963.68E-50.17ALG14Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
YBR192W3.797.52E-50.04RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YPR107C3.758.69E-50.35YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YDR091C3.403.38E-40.02RLI1Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species
YDR172W3.383.61E-40.03SUP35Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
YDR047W3.354.09E-40.15HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YMR033W3.196.99E-40.05ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YKL195W3.158.29E-40.02MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YJR065C3.138.89E-40.02ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR049C14.403.28E-47UTR1ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
YPR074C9.672.03E-22TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YJL121C6.331.22E-10RPE1D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
YNL058C_p5.872.19E-9YNL058C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene
YBR292C_d5.734.89E-9YBR292C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
YJL120W_d5.081.84E-7YJL120W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
YLR268W4.592.26E-6SEC22R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
YGR164W_d4.356.93E-6YGR164W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YMR135C4.308.52E-6GID8Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
YHR183W3.983.41E-5GND16-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
YNL012W3.934.28E-5SPO1Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
YIL052C3.865.69E-5RPL34BRibosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
YHR129C3.826.79E-5ARP1Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
YML081W3.711.02E-4TDA9DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
YOR209C3.701.07E-4NPT1Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus

GO enrichment analysis for SGTC_2223
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3231.88E-143SGTC_21395578692 56.5 μMChembridge (Fragment library)6509910.0428571superoxide
0.3005.02E-123SGTC_1771327-0068 8.4 μMChemDiv (Drug-like library)153899000.0857143
0.2301.15E-71SGTC_7991310-0557 151.0 μMChemDiv (Drug-like library)53544920.102941superoxide
0.2073.98E-58SGTC_27997812892 81.8 μMChembridge (Drug-like library)202052350.0909091superoxide
0.2061.23E-57SGTC_2233sulbentine 40.4 μMMiscellaneous676860.0923077
0.1971.83E-52SGTC_22147179297 48.3 μMChembridge (Fragment library)7697440.112903superoxide
0.1871.79E-47SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.0892857
0.1802.84E-44SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.0967742superoxide
0.1761.72E-42SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.106061superoxide
0.1661.66E-37SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.0967742superoxide
0.1646.79E-37SGTC_1831st055328 12.0 μMTimTec (Natural product derivative library)11030680.119403heme biosynthesis & mitochondrial translocase
0.1649.58E-37SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.025heme biosynthesis & mitochondrial translocase
0.1631.82E-36SGTC_20855307070 144.0 μMChembridge (Fragment library)28403510.0617284superoxide
0.1579.40E-34SGTC_23369001819 198.7 μMChembridge (Fragment library)29896020.129032heme biosynthesis & mitochondrial translocase
0.1532.29E-32SGTC_2681menadione 3.2 μMMiscellaneous40550.127273superoxide

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1994st07294740.9 μM0.3538465286565TimTec (Natural product derivative library)397.44914.74823
SGTC_7323970-172752.7 μM0.3055561275699ChemDiv (Drug-like library)416.4263.87115
SGTC_7263448-439134.2 μM0.2753621356692ChemDiv (Drug-like library)388.439044.43814
SGTC_1776st04924945.9 μM0.2753621822314TimTec (Natural product derivative library)435.473944.73613
SGTC_2466598340937.09 μM0.2537312879657Miscellaneous345.3448832.90316
SGTC_960929-009337.28 μM0.2424246991579ChemDiv (Drug-like library)332.7798034.64913calcium & mitochondrial duress
SGTC_1029k015-003064.7 μM0.2419352208316ChemDiv (Drug-like library)289.6888633.32903
SGTC_23427990637118.86 μM0.2419352033102Chembridge (Fragment library)242.23341.07123RNA processing & uracil transport
SGTC_2305744352269.23 μM0.2380955415886Chembridge (Fragment library)279.2901031.51625
SGTC_21365328288199.3 μM0.236364262395Chembridge (Fragment library)222.220540.81724