7312221

(E)-N-pyrimidin-2-yl-3-thiophen-2-ylprop-2-enamide

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2227
Screen concentration 64.1 μM
Source Chembridge (Fragment library)
PubChem CID 887188
SMILES C1=CN=C(N=C1)NC(=O)C=CC2=CC=CS2
Standardized SMILES O=C(Nc1ncccn1)C=Cc2cccs2
Molecular weight 231.2737
ALogP 1.78
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.17
% growth inhibition (Hom. pool) 3.81


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 887188
Download HIP data (tab-delimited text)  (excel)
Gene:CCT3(YJL014W)|FD-Score:-3.24|P-value:6.01E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GLN4(YOR168W)|FD-Score:19.3|P-value:2.33E-83|Clearance:15.52||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:YOR169C(YOR169C_d)|FD-Score:18.6|P-value:2.09E-77|Clearance:15.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:CCT3(YJL014W)|FD-Score:-3.24|P-value:6.01E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:GLN4(YOR168W)|FD-Score:19.3|P-value:2.33E-83|Clearance:15.52||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:YOR169C(YOR169C_d)|FD-Score:18.6|P-value:2.09E-77|Clearance:15.52||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 887188
Download HOP data (tab-delimited text)  (excel)
Gene:EMC4(YGL231C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GTO3(YMR251W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HSP82(YPL240C)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:NOP16(YER002W)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PEX7(YDR142C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PPT2(YPL148C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM2(YIL037C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PUB1(YNL016W)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RTC1(YOL138C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:UBP3(YER151C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YGR021W(YGR021W_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EMC4(YGL231C)|FD-Score:3.18|P-value:7.32E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85 Gene:GTO3(YMR251W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Omega class glutathione transferase; putative cytosolic localization Gene:HSP82(YPL240C)|FD-Score:4.57|P-value:2.39E-6||SGD DESC:Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication Gene:NOP16(YER002W)|FD-Score:-4.43|P-value:4.78E-6||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:PEX7(YDR142C)|FD-Score:3.15|P-value:8.22E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:PPT2(YPL148C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PRM2(YIL037C)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion Gene:PUB1(YNL016W)|FD-Score:-3.45|P-value:2.85E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:RTC1(YOL138C)|FD-Score:3.21|P-value:6.75E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif Gene:UBP3(YER151C)|FD-Score:3.89|P-value:5.03E-5||SGD DESC:Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress Gene:YGR021W(YGR021W_p)|FD-Score:3.78|P-value:7.97E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR168W19.302.33E-8315.50GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YOR169C_d18.602.09E-7715.50YOR169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
YGR099W3.070.001090.04TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YGL137W3.030.001240.15SEC27Essential beta'-coat protein of the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
YLR026C2.870.002040.20SED5cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
YDR062W2.670.003800.02LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YGL111W2.650.004080.08NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YGR120C2.570.005080.01COG2Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YOR074C2.560.005210.06CDC21Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
YGR048W2.500.006200.04UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YFR037C2.460.006900.00RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YCL041C_d2.460.006950.00YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YJR093C2.460.007030.02FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YMR211W2.440.007360.00DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YKL021C2.440.007400.01MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL240C4.572.39E-6HSP82Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
YER151C3.895.03E-5UBP3Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress
YMR251W3.826.66E-5GTO3Omega class glutathione transferase; putative cytosolic localization
YGR021W_p3.787.97E-5YGR021W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YPL148C3.275.47E-4PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YOL138C3.216.75E-4RTC1Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; null mutation suppresses cdc13-1 temperature sensitivity; has N-terminal WD-40 repeats and a C-terminal RING motif
YGL231C3.187.32E-4EMC4Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4, fly CG11137, human TMM85
YIL037C3.177.69E-4PRM2Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
YDR142C3.158.22E-4PEX7Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
YDR503C3.080.00102LPP1Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
YDR315C3.040.00118IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YLR297W_p3.020.00128YLR297W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
YLR432W3.000.00133IMD3Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed
YPR147C_p2.990.00141YPR147C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
YLR269C_d2.890.00191YLR269C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2227
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1563.52E-33SGTC_22376667747 47.2 μMChembridge (Fragment library)7299400.0806452
0.0961.85E-13SGTC_21806301957 200.0 μMChembridge (Fragment library)8454620.131148RSC complex & mRNA processing
0.0891.06E-11SGTC_21906049624 200.0 μMChembridge (Fragment library)22655540.0983607
0.0864.95E-11SGTC_1976st070609 63.5 μMTimTec (Natural product derivative library)6825560.0985916
0.0782.44E-9SGTC_1709st033179 39.1 μMTimTec (Natural product derivative library)5733810.0869565
0.0758.83E-9SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.086956560S ribosome export
0.0714.13E-8SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.126761RNA processing & uracil transport
0.0672.33E-7SGTC_2657nystatin 74.4 μMMiscellaneous114687070.0471698
0.0672.66E-7SGTC_31129123419 49.5 μMChembridge (Drug-like library)170158540.111111RPP1 & pyrimidine depletion
0.0664.40E-7SGTC_1762st045503 25.5 μMTimTec (Natural product derivative library)46455540.060241
0.0647.88E-7SGTC_7531322-0200 289.0 μMChemDiv (Drug-like library)54039070.134328
0.0631.24E-6SGTC_1759st043059 70.0 μMTimTec (Natural product derivative library)6694260.145161amide catabolism
0.0621.74E-6SGTC_424splitomicin 100.0 mMMiscellaneous52690.0877193
0.0622.07E-6SGTC_31879109394 49.5 μMChembridge (Drug-like library)170980900.241379Golgi
0.0613.07E-6SGTC_9711315-0384 26.7 μMChemDiv (Drug-like library)67691200.0689655cell wall signaling

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2061524588480.95 μM0.361702573863Chembridge (Fragment library)210.276041.56514tubulin folding & SWR complex
SGTC_14404100-155040 μM0.333333742806ChemDiv (Drug-like library)268.267382.23914
SGTC_610978-832798.57 μM0.3207556150059ChemDiv (Drug-like library)350.154412.5112ergosterol biosynthesis
SGTC_21365328288199.3 μM0.297872262395Chembridge (Fragment library)222.220540.81724
SGTC_6293448-646676.7 μM0.290909573744ChemDiv (Drug-like library)254.3072.75413
SGTC_23196033012152.6 μM0.288462847246Chembridge (Fragment library)219.262960.86524tubulin folding & SWR complex
SGTC_2065522159634.26 μM0.285714788581Chembridge (Fragment library)238.286141.71724
SGTC_12082323-234750.3 μM0.2769235412760ChemDiv (Drug-like library)346.405664.22724
SGTC_12092323-234750.3 μM0.2769235412760ChemDiv (Drug-like library)346.405664.22724
SGTC_242556286817.85 μM0.2727275714958Miscellaneous259.349964.02715
SGTC_305-(2-thienylidene)-Rhodanine28.7 μM0.2692311241132ChemDiv (Drug-like library)227.32642.79414