7354201

1'-(pyrrolidin-1-ylmethyl)spiro[1,3-dioxolane-2,3'-2H-indole]

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2228
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 753451
SMILES C1CCN(C1)CN2CC3(C4=CC=CC=C42)OCCO3
Standardized SMILES C(N1CCCC1)N2CC3(OCCO3)c4ccccc24
Molecular weight 260.3315
ALogP 2.06
H-bond donor count 0
H-bond acceptor count 4
Response signature endomembrane recycling

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.33
% growth inhibition (Hom. pool) 5.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 753451
Download HIP data (tab-delimited text)  (excel)
Gene:MAK11(YKL021C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.23||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:RPT5(YOR117W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC6(YCR052W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.23||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.23||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC26(YDR238C)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.23||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TOM40(YMR203W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.23||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:MAK11(YKL021C)|FD-Score:3.52|P-value:2.12E-4|Clearance:0.23||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:RPT5(YOR117W)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC6(YCR052W)|FD-Score:3.46|P-value:2.73E-4|Clearance:0.23||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:3.8|P-value:7.26E-5|Clearance:0.23||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:SEC26(YDR238C)|FD-Score:3.18|P-value:7.35E-4|Clearance:0.23||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TOM40(YMR203W)|FD-Score:3.28|P-value:5.25E-4|Clearance:0.23||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 753451
Download HOP data (tab-delimited text)  (excel)
Gene:ADY4(YLR227C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:ALG9(YNL219C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ARR3(YPR201W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:CST9(YLR394W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:LDB7(YBL006C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:PEX7(YDR142C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RCY1(YJL204C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SAC1(YKL212W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:VID24(YBR105C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS61(YDR136C_d)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YKL075C(YKL075C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin Gene:ADY4(YLR227C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:ALG9(YNL219C)|FD-Score:3.19|P-value:7.21E-4||SGD DESC:Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation Gene:ARR3(YPR201W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite Gene:CST9(YLR394W)|FD-Score:3.37|P-value:3.74E-4||SGD DESC:SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate Gene:LDB7(YBL006C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:PEX7(YDR142C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP) Gene:QRI5(YLR204W)|FD-Score:3.24|P-value:5.92E-4||SGD DESC:Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA Gene:RCY1(YJL204C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:SAC1(YKL212W)|FD-Score:3.24|P-value:5.90E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:VID24(YBR105C)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VPS61(YDR136C_d)|FD-Score:-3.86|P-value:5.71E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YKL075C(YKL075C_p)|FD-Score:3.13|P-value:8.75E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR037C3.807.26E-50.23RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YOR117W3.691.13E-40.23RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YKL021C3.522.12E-40.23MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
YCR052W3.462.73E-40.23RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YMR203W3.285.25E-40.23TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YDR238C3.187.35E-40.23SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YER006W2.950.001583.44E-4NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YGR048W2.950.001590.01UFD1Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transaction factors by regulated Ub/proteasome-dependent processing (RUP)
YGR099W2.950.001610.02TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YDR394W2.930.001700.01RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YOR319W2.920.001770.05HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YGL055W2.870.002070.09OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YNL062C2.770.002770.00GCD10Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
YMR128W2.770.002790.06ECM16Essential DEAH-box ATP-dependent RNA helicase specific to the U3 snoRNP, predominantly nucleolar in distribution, required for 18S rRNA synthesis
YKL141W2.710.003330.04SDH3Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL204C4.661.58E-6RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YLR227C4.072.33E-5ADY4Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane
YBL006C4.072.35E-5LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YDR142C3.711.05E-4PEX7Peroxisomal signal receptor for the N-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins; WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
YLR394W3.373.74E-4CST9SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate
YKL212W3.245.90E-4SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YLR204W3.245.92E-4QRI5Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
YPR201W3.236.21E-4ARR3Plasma membrane metalloid/H+ antiporter; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite
YNL219C3.197.21E-4ALG9Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
YKL075C_p3.138.75E-4YKL075C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; proposed to be involved in resistance to streptozotocin and camptothecin
YBR105C3.109.64E-4VID24GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
YDR315C3.030.00120IPK1Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
YDR234W3.020.00128LYS4Homoaconitase, catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
YOR243C3.000.00137PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YLR280C_d2.980.00146YLR280C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2228
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2254.38E-68SGTC_9541189-0853 2.9 μMChemDiv (Drug-like library)28281940.1endomembrane recycling
0.2201.65E-65SGTC_23749071832 200.0 μMChembridge (Fragment library)173816980.0892857endomembrane recycling
0.1911.07E-49SGTC_13943474-0001 65.7 μMChemDiv (Drug-like library)50176660.0895522endomembrane recycling
0.1911.89E-49SGTC_23809035439 200.0 μMChembridge (Fragment library)64625750.0727273endomembrane recycling
0.1792.21E-43SGTC_830170-0301 39.0 μMChemDiv (Drug-like library)30897160.0172414endomembrane recycling
0.1759.30E-42SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.189655fatty acid desaturase (OLE1)
0.1681.16E-38SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.148148heme biosynthesis & mitochondrial translocase
0.1612.52E-35SGTC_218alverine citrate 93.8 μMMiscellaneous217180.117647fatty acid desaturase (OLE1)
0.1604.08E-35SGTC_1102cgs 12066b 26.1 μMNIH Clinical Collection64383520.1endomembrane recycling
0.1591.65E-34SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.180328fatty acid desaturase (OLE1)
0.1562.41E-33SGTC_2694st077685 81.9 μMTimTec (Natural product derivative library)28380960.0857143endomembrane recycling
0.1541.73E-32SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.136364fatty acid desaturase (OLE1)
0.1511.59E-31SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.0819672mitochondrial processes
0.1484.61E-30SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.149254fatty acid desaturase (OLE1)
0.1471.11E-29SGTC_29939054257 71.4 μMChembridge (Drug-like library)64702170.163934fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21055350118200 μM0.3076922133313Chembridge (Fragment library)249.3322.69913
SGTC_11720370-004385 μM0.25376585ChemDiv (Drug-like library)355.520145.28504
SGTC_9001112-0045141 μM0.24074156211ChemDiv (Drug-like library)322.443944.5312NEO1
SGTC_21495554786194.83 μM0.236364745063Chembridge (Fragment library)230.308821.94613cell wall signaling
SGTC_20665222451176 μM0.2321432281147Chembridge (Fragment library)242.273161.60503heme biosynthesis & mitochondrial translocase
SGTC_20955432005200 μM0.229167783734Chembridge (Fragment library)204.31132.52602
SGTC_23579067248200 μM0.2264159150235Chembridge (Fragment library)225.628421.18413
SGTC_13561502-078185.8 μM0.224138567702ChemDiv (Drug-like library)284.761823.53804fatty acid desaturase (OLE1)
SGTC_14801070-004441.2 μM0.2222223124987ChemDiv (Drug-like library)294.390783.41702
SGTC_20935331342194.4 μM0.21818233839Chembridge (Fragment library)243.300982.25713