7354935

2-(4-aminophenyl)-1H-quinazolin-4-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2229
Screen concentration 190.0 μM
Source Chembridge (Fragment library)
PubChem CID 318112
SMILES C1=CC=C2C(=C1)C(=O)N=C(N2)C3=CC=C(C=C3)N
Standardized SMILES Nc1ccc(cc1)c2nc(O)c3ccccc3n2
Molecular weight 237.2566
ALogP 1.75
H-bond donor count 2
H-bond acceptor count 2
Response signature RSC complex & mRNA processing

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.45
% growth inhibition (Hom. pool) 8.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 318112
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:5.87|P-value:2.15E-9|Clearance:0.02||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC40(YBR234C)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:7.78|P-value:3.68E-15|Clearance:0.56||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:10.3|P-value:2.42E-25|Clearance:0.5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:4.89|P-value:5.03E-7|Clearance:0.95||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DFR1(YOR236W)|FD-Score:7.05|P-value:8.87E-13|Clearance:0.12||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:GFA1(YKL104C)|FD-Score:3.94|P-value:4.13E-5|Clearance:0.17||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:7.22|P-value:2.59E-13|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCD4(YKL165C)|FD-Score:3.14|P-value:8.37E-4|Clearance:0.1||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:PAB1(YER165W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:6.7|P-value:1.06E-11|Clearance:0.12||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIB2(YOL066C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:3.76|P-value:8.38E-5|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB7(YDR404C)|FD-Score:5.91|P-value:1.74E-9|Clearance:0.03||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.37||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPR2(YIR015W)|FD-Score:6.58|P-value:2.39E-11|Clearance:0.67||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:12.3|P-value:3.94E-35|Clearance:1.98||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:9.84|P-value:3.75E-23|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:9.25|P-value:1.08E-20|Clearance:1.48||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN1(YLR430W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.12||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPB4(YFL002C)|FD-Score:3.76|P-value:8.60E-5|Clearance:0.07||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFA1(YKL028W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.12||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:WRS1(YOL097C)|FD-Score:5.85|P-value:2.42E-9|Clearance:0.49||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YSH1(YLR277C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.22||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:YTH1(YPR107C)|FD-Score:6.93|P-value:2.04E-12|Clearance:0.24||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ACC1(YNR016C)|FD-Score:5.87|P-value:2.15E-9|Clearance:0.02||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARC40(YBR234C)|FD-Score:3.68|P-value:1.14E-4|Clearance:0.06||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP4(YJL081C)|FD-Score:3.69|P-value:1.12E-4|Clearance:0.01||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:ARP7(YPR034W)|FD-Score:7.78|P-value:3.68E-15|Clearance:0.56||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:10.3|P-value:2.42E-25|Clearance:0.5||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC24(YAL041W)|FD-Score:4.89|P-value:5.03E-7|Clearance:0.95||SGD DESC:Guanine nucleotide exchange factor for Cdc42p; also known as a GEF or GDP-release factor; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:DFR1(YOR236W)|FD-Score:7.05|P-value:8.87E-13|Clearance:0.12||SGD DESC:Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism Gene:GFA1(YKL104C)|FD-Score:3.94|P-value:4.13E-5|Clearance:0.17||SGD DESC:Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication Gene:LSM2(YBL026W)|FD-Score:7.22|P-value:2.59E-13|Clearance:0.17||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:MCD4(YKL165C)|FD-Score:3.14|P-value:8.37E-4|Clearance:0.1||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:PAB1(YER165W)|FD-Score:3.27|P-value:5.42E-4|Clearance:0.01||SGD DESC:Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G Gene:PKC1(YBL105C)|FD-Score:6.7|P-value:1.06E-11|Clearance:0.12||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RIB2(YOL066C)|FD-Score:3.51|P-value:2.20E-4|Clearance:0.03||SGD DESC:Bifunctional enzyme with DRAP deaminase and tRNA:pseudouridine synthase activity; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs Gene:RPB10(YOR210W)|FD-Score:3.76|P-value:8.38E-5|Clearance:0.01||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB7(YDR404C)|FD-Score:5.91|P-value:1.74E-9|Clearance:0.03||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPB8(YOR224C)|FD-Score:5.36|P-value:4.13E-8|Clearance:0.37||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL32(YBL092W)|FD-Score:4.99|P-value:2.95E-7|Clearance:0.1||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:RPR2(YIR015W)|FD-Score:6.58|P-value:2.39E-11|Clearance:0.67||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSC58(YLR033W)|FD-Score:12.3|P-value:3.94E-35|Clearance:1.98||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:9.84|P-value:3.75E-23|Clearance:0.59||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:9.25|P-value:1.08E-20|Clearance:1.48||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEN1(YLR430W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.12||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SPB4(YFL002C)|FD-Score:3.76|P-value:8.60E-5|Clearance:0.07||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:TFA1(YKL028W)|FD-Score:3.26|P-value:5.58E-4|Clearance:0.12||SGD DESC:TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening Gene:WRS1(YOL097C)|FD-Score:5.85|P-value:2.42E-9|Clearance:0.49||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YSH1(YLR277C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.22||SGD DESC:Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication Gene:YTH1(YPR107C)|FD-Score:6.93|P-value:2.04E-12|Clearance:0.24||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 318112
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMP2'(YIL154C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LDB7(YBL006C)|FD-Score:9.74|P-value:1.04E-22||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAL13(YGR288W)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MMM1(YLL006W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:NMA111(YNL123W)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP100(YKL068W)|FD-Score:5.87|P-value:2.15E-9||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PFK2(YMR205C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PSY2(YNL201C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD57(YDR004W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPN4(YDL020C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0B(YLR048W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:SOH1(YGL127C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRC1(YML034W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:ULS1(YOR191W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VMA1(YDL185W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YCR064C(YCR064C_d)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL037C(YHL037C_d)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR202W(YHR202W_p)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR115W(YJR115W_p)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:ZAP1(YJL056C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains Gene:ACB1(YGR037C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:HTA1(YDR225W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:IMP2'(YIL154C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:LDB7(YBL006C)|FD-Score:9.74|P-value:1.04E-22||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:MAL13(YGR288W)|FD-Score:4.31|P-value:8.24E-6||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MMM1(YLL006W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:NMA111(YNL123W)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NUP100(YKL068W)|FD-Score:5.87|P-value:2.15E-9||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p Gene:PFK2(YMR205C)|FD-Score:-3.55|P-value:1.96E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PSY2(YNL201C)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:RAD57(YDR004W)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:RPN4(YDL020C)|FD-Score:4.19|P-value:1.42E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS0B(YLR048W)|FD-Score:-3.26|P-value:5.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2 Gene:SOH1(YGL127C)|FD-Score:4.07|P-value:2.35E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SRC1(YML034W)|FD-Score:3.76|P-value:8.47E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:ULS1(YOR191W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:VMA1(YDL185W)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:YCR064C(YCR064C_d)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YCR102W-A(YCR102W-A_d)|FD-Score:3.39|P-value:3.53E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHL037C(YHL037C_d)|FD-Score:3.31|P-value:4.67E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YHR202W(YHR202W_p)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization Gene:YJR115W(YJR115W_p)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:ZAP1(YJL056C)|FD-Score:-3.2|P-value:6.95E-4||SGD DESC:Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR033W12.303.94E-351.98RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YMR033W10.302.42E-250.49ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YFR037C9.843.75E-230.59RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YML127W9.251.08E-201.48RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YPR034W7.783.68E-150.56ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YBL026W7.222.59E-130.17LSM2Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YOR236W7.058.87E-130.12DFR1Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
YPR107C6.932.04E-120.24YTH1Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
YBL105C6.701.06E-110.12PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YIR015W6.582.39E-110.67RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YDR404C5.911.74E-90.03RPB7RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
YNR016C5.872.15E-90.02ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YOL097C5.852.42E-90.49WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YOR224C5.364.13E-80.37RPB8RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III
YBL092W4.992.95E-70.10RPL32Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBL006C9.741.04E-22LDB7Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
YKL068W5.872.15E-9NUP100FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; homologous to Nup116p
YHR202W_p5.257.55E-8YHR202W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
YNL123W4.415.21E-6NMA111Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases
YGR288W4.318.24E-6MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YDR225W4.251.09E-5HTA1Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
YDL020C4.191.42E-5RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YIL154C4.171.53E-5IMP2'Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat
YGL127C4.072.35E-5SOH1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
YDR004W4.022.95E-5RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YML034W3.768.47E-5SRC1Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
YPR039W_d3.721.01E-4YPR039W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W
YGR037C3.701.06E-4ACB1Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
YOR191W3.462.75E-4ULS1Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
YCR102W-A_d3.393.53E-4YCR102W-A_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2229
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.4850SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0588235RSC complex & mRNA processing
0.4710SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.14RSC complex & mRNA processing
0.4690SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.166667RSC complex & mRNA processing
0.4365.22E-272SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.0597015RSC complex & mRNA processing
0.3995.52E-224SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0606061RSC complex & mRNA processing
0.3911.20E-214SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0363636RSC complex & mRNA processing
0.3752.81E-196SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.0606061RSC complex & mRNA processing
0.3704.18E-190SGTC_10503232-1156 69.3 μMChemDiv (Drug-like library)7005290.142857RSC complex & mRNA processing
0.3601.63E-179SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0441176RSC & ERG11
0.3597.12E-179SGTC_23619034121 116.0 μMChembridge (Fragment library)64969170.166667RSC complex & mRNA processing
0.3463.40E-165SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.14RSC complex & mRNA processing
0.3351.76E-154SGTC_21115486399 200.0 μMChembridge (Fragment library)6896740.0877193
0.3291.77E-148SGTC_8291635-0120 117.0 μMChemDiv (Drug-like library)2850540.115385DNA damage response
0.3202.80E-140SGTC_248streptozotocin 5.2 mMMiscellaneous53000.0151515DNA damage response
0.3183.80E-138SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.0833333RSC complex & mRNA processing

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_13551499-00351.68 μM0.4047621548538ChemDiv (Drug-like library)241.073441.97211
SGTC_3252913587149.47 μM0.37254917682838Chembridge (Drug-like library)245.27711.51122
SGTC_22127123476200 μM0.3111111085715Chembridge (Fragment library)204.291382.85711
SGTC_21775811945192 μM0.3061225343475Chembridge (Fragment library)201.224540.91212
SGTC_21685727979200 μM0.304348695210Chembridge (Fragment library)206.26422.23603
SGTC_21785812956200 μM0.35403383Chembridge (Fragment library)215.251121.49212
SGTC_1678st01926580.2 μM0.2916675367020TimTec (Natural product derivative library)249.264043.6080360S ribosome export
SGTC_930109-004559.23 μM0.28888988733ChemDiv (Drug-like library)210.23132.53413DNA damage response
SGTC_3393091-483371.4 μM0.2884623240077ChemDiv (Drug-like library)272.686463.1713iron homeostasis
SGTC_13853091-483414.1 μM0.2884624072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion