busulfan

4-methylsulfonyloxybutyl methanesulfonate

A DNA alkylator.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Alkylating Agents;Antineoplastic Agents, Alkylating;Immunosuppressive Agents;Myeloablative Agonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_223
Screen concentration 5.0 mM
Source Miscellaneous
PubChem CID 2478
SMILES CS(=O)(=O)OCCCCOS(=O)(=O)C
Standardized SMILES CS(=O)(=O)OCCCCOS(=O)(=O)C
Molecular weight 246.3018
ALogP -0.29
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.8
% growth inhibition (Hom. pool) -1.33


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2478
Download HIP data (tab-delimited text)  (excel)
Gene:CDC12(YHR107C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.04||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:EPL1(YFL024C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.09||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:MEC1(YBR136W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.07||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NUP49(YGL172W)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.27||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP82(YJL061W)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PRP45(YAL032C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.14||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:TFB2(YPL122C)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.02||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB4(YLR212C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.34||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBC1(YDR177W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJL086C(YJL086C_d)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YPR136C(YPR136C_d)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9 Gene:CDC12(YHR107C)|FD-Score:3.58|P-value:1.69E-4|Clearance:0.04||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:EPL1(YFL024C)|FD-Score:3.55|P-value:1.93E-4|Clearance:0.09||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:MEC1(YBR136W)|FD-Score:3.44|P-value:2.94E-4|Clearance:0.07||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NUP49(YGL172W)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.27||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:NUP82(YJL061W)|FD-Score:-3.58|P-value:1.73E-4|Clearance:0||SGD DESC:Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) Gene:PRP45(YAL032C)|FD-Score:3.36|P-value:3.84E-4|Clearance:0.14||SGD DESC:Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene Gene:TFB2(YPL122C)|FD-Score:3.46|P-value:2.71E-4|Clearance:0.02||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TUB4(YLR212C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0.34||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:UBC1(YDR177W)|FD-Score:3.46|P-value:2.66E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:3.65|P-value:1.31E-4|Clearance:0.07||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YJL086C(YJL086C_d)|FD-Score:-3.36|P-value:3.95E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YPR136C(YPR136C_d)|FD-Score:3.23|P-value:6.29E-4|Clearance:0.16||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2478
Download HOP data (tab-delimited text)  (excel)
Gene:AVT1(YJR001W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:COG1(YGL223C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTS2(YDR371W_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:EFM1(YHL039W)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FAP1(YNL023C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FMP40(YPL222W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:6.42|P-value:6.92E-11||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:JEM1(YJL073W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JNM1(YMR294W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:NTC20(YBR188C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OST6(YML019W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PHO89(YBR296C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:RAD5(YLR032W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD57(YDR004W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:REV3(YPL167C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPS23B(YPR132W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SLZ1(YNL196C_p)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SOL3(YHR163W)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:UBR1(YGR184C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:XBP1(YIL101C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL004W(YAL004W_d)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR013C(YBR013C_p)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR029W(YDR029W_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR050C(YGR050C_p)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR271W(YLR271W_p)|FD-Score:13.5|P-value:1.44E-41||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR456W(YLR456W_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YNL295W(YNL295W_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:AVT1(YJR001W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:COG1(YGL223C)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTS2(YDR371W_p)|FD-Score:3.27|P-value:5.37E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:EFM1(YHL039W)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:FAP1(YNL023C)|FD-Score:-3.68|P-value:1.17E-4||SGD DESC:Protein that binds to Fpr1p, conferring rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1 Gene:FMP40(YPL222W_p)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:6.42|P-value:6.92E-11||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:JEM1(YJL073W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2 Gene:JNM1(YMR294W)|FD-Score:3.63|P-value:1.41E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:KAP120(YPL125W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:NTC20(YBR188C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:OST6(YML019W)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p Gene:PHO89(YBR296C)|FD-Score:3.8|P-value:7.30E-5||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:RAD5(YLR032W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress Gene:RAD57(YDR004W)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p Gene:REV3(YPL167C)|FD-Score:3.15|P-value:8.25E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPS23B(YPR132W)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:SLZ1(YNL196C_p)|FD-Score:3.85|P-value:5.85E-5||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SOL3(YHR163W)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:STE50(YCL032W)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:UBR1(YGR184C)|FD-Score:-3.24|P-value:5.94E-4||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:XBP1(YIL101C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Transcriptional repressor; binds to promoter sequences of the cyclin genes, CYS3, and SMF2; expression is induced by stress or starvation during mitosis, and late in meiosis; member of the Swi4p/Mbp1p family; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:YAL004W(YAL004W_d)|FD-Score:-3.15|P-value:8.25E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C Gene:YBR013C(YBR013C_p)|FD-Score:4.44|P-value:4.44E-6||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YDR029W(YDR029W_d)|FD-Score:-3.12|P-value:9.09E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR050C(YGR050C_p)|FD-Score:3.76|P-value:8.42E-5||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YLR271W(YLR271W_p)|FD-Score:13.5|P-value:1.44E-41||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR456W(YLR456W_p)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Putative pyridoxal 5'-phosphate synthase; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication Gene:YNL295W(YNL295W_p)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function Gene:YPL257W(YPL257W_p)|FD-Score:-3.17|P-value:7.50E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR212C4.261.03E-50.34TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YGL172W3.924.48E-50.27NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YDR187C_d3.651.31E-40.07YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YHR107C3.581.69E-40.04CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YFL024C3.551.93E-40.09EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YDR177W3.462.66E-40.00UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YPL122C3.462.71E-40.02TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YBR136W3.442.94E-40.07MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YAL032C3.363.84E-40.14PRP45Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
YPR136C_d3.236.29E-40.16YPR136C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF RRP9
YOR372C3.070.001080.08NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YOR260W2.990.001400.07GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YIR006C2.910.001780.03PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YJR123W2.880.001980.06RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YDL208W2.820.002420.11NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR271W_p13.501.44E-41YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YGL220W6.426.92E-11FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YHR163W5.501.89E-8SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YBR013C_p4.444.44E-6YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YGL223C4.102.04E-5COG1Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YLR032W4.092.17E-5RAD5DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
YDR004W3.953.92E-5RAD57Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
YNL196C_p3.855.85E-5SLZ1_pSporulation-specific protein with a leucine zipper motif
YBR296C3.807.30E-5PHO89Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
YGR050C_p3.768.42E-5YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YBR188C3.691.12E-4NTC20Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YMR294W3.631.41E-4JNM1Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
YJR001W3.631.41E-4AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YJL073W3.442.87E-4JEM1DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
YPL222W_p3.363.95E-4FMP40_pPutative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_223
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3681.44E-188SGTC_225nsc-122657 1.0 μMMiscellaneous2756850.0888889
0.2899.38E-114SGTC_220paclitaxel 373.4 μMMiscellaneous46660.030303
0.2707.18E-99SGTC_224benfluorex 43.9 μMMiscellaneous23180.0892857
0.2494.04E-84SGTC_7734358-1457 44.6 μMChemDiv (Drug-like library)11868860.0714286
0.2113.55E-60SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.02
0.1376.68E-26SGTC_219mitoxanthrone 164.4 μMMiscellaneous42120.0454545DNA intercalators
0.1063.74E-16SGTC_218alverine citrate 93.8 μMMiscellaneous217180.0857143fatty acid desaturase (OLE1)
0.0954.06E-13SGTC_2113455-0679 143.5 μMChemDiv (Drug-like library)7486490.0851064
0.0903.40E-12SGTC_1563474-0145 5.6 μMChemDiv (Drug-like library)22654710.0666667endomembrane recycling
0.0822.80E-10SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)15993494NA
0.0807.38E-10SGTC_217ketoconazole 989.0 nMMiscellaneous4562010.0493827ergosterol depletion effects on membrane
0.0791.38E-9SGTC_29035923464 63.9 μMChembridge (Drug-like library)28765760.0555556
0.0715.50E-8SGTC_1653st012112 65.0 μMTimTec (Natural product derivative library)7204290.0816327
0.0672.56E-7SGTC_287k060-0039 29.6 μMChemDiv (Drug-like library)5861974NA
0.0663.70E-7SGTC_23955646831 180.2 μMMiscellaneous68086280.0285714

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_915methyl methanesulfonate (MMS)110 mM0.3888894156Miscellaneous110.13224-0.37503DNA damage response
SGTC_2741miltefosine3.04 μM0.1944443599Miscellaneous407.5680023.72204
SGTC_269exalamide29 μM0.1753316Miscellaneous221.295463.05612ergosterol biosynthesis
SGTC_1632st00565090 μM0.1707322759605TimTec (Natural product derivative library)222.280223.34103
SGTC_2248tofa1.1 μM0.170732115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_2416tofa880 nM0.170732115175Miscellaneous324.454986.85314sphingolipid biosynthesis & PDR1
SGTC_2501sodium tetradecyl sulfate13.37 μM0.17073223665772Microsource (Natural product library)316.4324292.65804
SGTC_3230913166549.47 μM0.17073215943720Chembridge (Drug-like library)257.349162.28904
SGTC_3314913586059.43 μM0.16326525252478Chembridge (Drug-like library)346.872722.8805
SGTC_1290088-0017176.64 μM0.162791182446ChemDiv (Drug-like library)278.343483.63914
SGTC_13141222-001041.1 μM0.1627913526027ChemDiv (Drug-like library)251.321443.98514