bromochlorosalicylanilide

5-bromo-N-(4-chlorophenyl)-2-hydroxybenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2230
Screen concentration 954.5 nM
Source Miscellaneous
PubChem CID 77254
SMILES C1=CC(=CC=C1NC(=O)C2=C(C=CC(=C2)Br)O)Cl
Standardized SMILES Oc1ccc(Br)cc1C(=O)Nc2ccc(Cl)cc2
Molecular weight 326.5731
ALogP 3.79
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 28.21
% growth inhibition (Hom. pool) 4.27


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 77254
Download HIP data (tab-delimited text)  (excel)
Gene:CDC20(YGL116W)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DIM1(YPL266W)|FD-Score:3.26|P-value:5.50E-4|Clearance:0.02||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.37||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG1(YGR175C)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:GCD11(YER025W)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:ILV5(YLR355C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.29||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-5.07|P-value:2.03E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:4.6|P-value:2.09E-6|Clearance:0.57||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:PUP3(YER094C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RIM2(YBR192W)|FD-Score:4.03|P-value:2.80E-5|Clearance:0.47||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB8(YOR224C)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS20(YHL015W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SEC23(YPR181C)|FD-Score:-3.13|P-value:8.76E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SUI2(YJR007W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM40(YMR203W)|FD-Score:5.12|P-value:1.53E-7|Clearance:0.57||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:CDC20(YGL116W)|FD-Score:-3.61|P-value:1.51E-4|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDS1(YBR029C)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids Gene:DIM1(YPL266W)|FD-Score:3.26|P-value:5.50E-4|Clearance:0.02||SGD DESC:Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing Gene:DML1(YMR211W)|FD-Score:3.24|P-value:5.94E-4|Clearance:0.37||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:ERG1(YGR175C)|FD-Score:3.27|P-value:5.40E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:GCD11(YER025W)|FD-Score:-3.14|P-value:8.57E-4|Clearance:0||SGD DESC:Gamma subunit of the translation initiation factor eIF2, involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met Gene:ILV5(YLR355C)|FD-Score:3.56|P-value:1.84E-4|Clearance:0.29||SGD DESC:Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids Gene:IRA1(YBR140C)|FD-Score:-5.07|P-value:2.03E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:LSM4(YER112W)|FD-Score:4.6|P-value:2.09E-6|Clearance:0.57||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress Gene:PUP3(YER094C)|FD-Score:-3.11|P-value:9.50E-4|Clearance:0||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RIM2(YBR192W)|FD-Score:4.03|P-value:2.80E-5|Clearance:0.47||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB8(YOR224C)|FD-Score:-3.42|P-value:3.17E-4|Clearance:0||SGD DESC:RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III Gene:RPL5(YPL131W)|FD-Score:-3.26|P-value:5.60E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPS20(YHL015W)|FD-Score:-3.27|P-value:5.30E-4|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10 Gene:SEC23(YPR181C)|FD-Score:-3.13|P-value:8.76E-4|Clearance:0||SGD DESC:GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p Gene:SUI2(YJR007W)|FD-Score:-4.02|P-value:2.88E-5|Clearance:0||SGD DESC:Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress Gene:SUI3(YPL237W)|FD-Score:-3.49|P-value:2.45E-4|Clearance:0||SGD DESC:Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding Gene:TOM40(YMR203W)|FD-Score:5.12|P-value:1.53E-7|Clearance:0.57||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 77254
Download HOP data (tab-delimited text)  (excel)
Gene:ACA1(YER045C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE4(YMR300C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:CWC27(YPL064C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:INP53(YOR109W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IRS4(YKR019C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.81|P-value:6.91E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SLM5(YCR024C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SNQ2(YDR011W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TIM8(YJR135W-A)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:YCL002C(YCL002C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:12|P-value:2.11E-33||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:ACA1(YER045C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:3.54|P-value:1.99E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADE4(YMR300C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:CWC27(YPL064C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:INP53(YOR109W)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains Gene:IRS4(YKR019C)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-3.81|P-value:6.91E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SLM5(YCR024C)|FD-Score:3.91|P-value:4.65E-5||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SNQ2(YDR011W)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:SUR4(YLR372W)|FD-Score:5.14|P-value:1.36E-7||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWA2(YDR320C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TIM8(YJR135W-A)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:YCL002C(YCL002C_p)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YPK1(YKL126W)|FD-Score:12|P-value:2.11E-33||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR203W5.121.53E-70.57TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YER112W4.602.09E-60.57LSM4Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
YBR192W4.032.80E-50.47RIM2Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
YLR355C3.561.84E-40.29ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YGR175C3.275.40E-40.01ERG1Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine
YPL266W3.265.50E-40.02DIM1Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
YMR211W3.245.94E-40.37DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YOR232W2.880.002010.24MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YCR052W2.640.004200.07RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YKL192C2.560.005200.04ACP1Mitochondrial matrix acyl carrier protein, involved in biosynthesis of octanoate, which is a precursor to lipoic acid; activated by phosphopantetheinylation catalyzed by Ppt2p
YDL126C2.520.005820.02CDC48AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
YIR012W2.500.006180.02SQT1Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress
YBL105C2.480.006510.06PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YHR058C2.420.007740.02MED6Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
YDR037W2.400.008120.07KRS1Lysyl-tRNA synthetase

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL126W12.002.11E-33YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YLR372W5.141.36E-7SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YDR320C4.141.70E-5SWA2Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
YKR019C3.983.50E-5IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YCR024C3.914.65E-5SLM5Mitochondrial asparaginyl-tRNA synthetase
YOR109W3.865.61E-5INP53Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
YPL064C3.661.24E-4CWC27Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
YLR304C3.541.99E-4ACO1Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy
YMR300C3.542.02E-4ADE4Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
YER045C3.462.66E-4ACA1ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
YJR135W-A3.452.83E-4TIM8Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome
YDR011W3.265.58E-4SNQ2Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
YCL002C_p3.187.34E-4YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YMR271C3.070.00107URA10Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
YHR157W3.070.00107REC104Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination

GO enrichment analysis for SGTC_2230
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2775.05E-104SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.0487805
0.2681.39E-96SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.219178
0.2676.81E-97SGTC_2766oltipraz 83.9 μMMiscellaneous473180.0338983heme requiring
0.2495.71E-84SGTC_29809014342 25.3 μMChembridge (Drug-like library)29965990.277778
0.2478.44E-83SGTC_7243910-0327 137.0 μMChemDiv (Drug-like library)28854830.136364ERG2
0.2441.57E-80SGTC_14103909-8106 77.9 μMChemDiv (Drug-like library)10392100.0632911
0.2392.60E-77SGTC_1785bathophenanthroline 24.0 μMMiscellaneous728120.0576923heme requiring
0.2278.87E-70SGTC_24595811157 9.2 μMMiscellaneous28706840.222222mitochondrial processes
0.2232.13E-67SGTC_1301naphtanilide LB 4.8 μMChemDiv (Drug-like library)672380.510204heme requiring
0.2023.95E-55SGTC_2740felodipine 17.1 μMMiscellaneous33330.117647
0.2011.96E-54SGTC_14224031-0024 15.5 μMChemDiv (Drug-like library)7824270.20339iron homeostasis
0.1992.36E-53SGTC_1240136-0217 7.1 μMChemDiv (Drug-like library)39769050.25mitochondrial stress
0.1952.37E-51SGTC_14504112-4260 37.6 μMChemDiv (Drug-like library)16331750.0675676calcium & mitochondrial duress
0.1911.29E-49SGTC_6341611-4317 11.2 μMChemDiv (Drug-like library)28435220.180328ERG2
0.1839.46E-46SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.107692heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_700141-028943.28 μM0.675352408ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_1301naphtanilide LB4.81 μM0.51020467238ChemDiv (Drug-like library)336.771724.5332heme requiring
SGTC_1190121-004324.82 μM0.4893621092378ChemDiv (Drug-like library)405.469124.53322mitochondrial stress
SGTC_2872904403725.97 μM0.4883726465195Chembridge (Drug-like library)284.1131234.14912
SGTC_1310141-027733.85 μM0.448981547771ChemDiv (Drug-like library)419.49575.0222
SGTC_3650121-005213.6 μM0.448983101739ChemDiv (Drug-like library)361.564684.2624mitochondrial response to ROS
SGTC_7950685-026578 μM0.434783302754ChemDiv (Drug-like library)340.599643.86212excess fatty acid
SGTC_6541124-027180.9 μM0.4230775403748ChemDiv (Drug-like library)309.115462.58524
SGTC_12201838-0075193 μM0.45529645ChemDiv (Drug-like library)379.20533.15625
SGTC_310508-197457.24 μM0.3928576810525ChemDiv (Drug-like library)414.679724.6213