benfluorex

2-[1-[3-(trifluoromethyl)phenyl]propan-2-ylamino]ethyl benzoate

Inhibits enzymes in fatty acid oxidation, ketogenisis and gluconeogenesis.

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PubChem MeSH terms: Appetite Depressants;Hypolipidemic Agents



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_224
Screen concentration 43.9 μM
Source Miscellaneous
PubChem CID 2318
SMILES CC(CC1=CC(=CC=C1)C(F)(F)F)NCCOC(=O)C2=CC=CC=C2
Standardized SMILES CC(Cc1cccc(c1)C(F)(F)F)NCCOC(=O)c2ccccc2
Molecular weight 351.3628
ALogP 4.51
H-bond donor count 1
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.27
% growth inhibition (Hom. pool) 8.48


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2318
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG1(YBR110W)|FD-Score:3.9|P-value:4.90E-5|Clearance:0.03||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:HEM12(YDR047W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.22||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:KEI1(YDR367W)|FD-Score:-3.45|P-value:2.76E-4|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:NOP56(YLR197W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:OLE1(YGL055W)|FD-Score:5.96|P-value:1.30E-9|Clearance:1.54||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.42|P-value:5.04E-6|Clearance:0.41||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPS5(YJR123W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC26(YDR238C)|FD-Score:3.09|P-value:9.88E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TOM40(YMR203W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:7.96|P-value:8.49E-16|Clearance:2.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL142C(YPL142C_d)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit Gene:ALA1(YOR335C)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:ALG1(YBR110W)|FD-Score:3.9|P-value:4.90E-5|Clearance:0.03||SGD DESC:Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog Gene:HEM12(YDR047W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.22||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:KEI1(YDR367W)|FD-Score:-3.45|P-value:2.76E-4|Clearance:0||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:NOP56(YLR197W)|FD-Score:3.27|P-value:5.31E-4|Clearance:0.18||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:OLE1(YGL055W)|FD-Score:5.96|P-value:1.30E-9|Clearance:1.54||SGD DESC:Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria Gene:PKC1(YBL105C)|FD-Score:4.42|P-value:5.04E-6|Clearance:0.41||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RPS5(YJR123W)|FD-Score:4.01|P-value:3.08E-5|Clearance:0.11||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC26(YDR238C)|FD-Score:3.09|P-value:9.88E-4|Clearance:0.06||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:TOM40(YMR203W)|FD-Score:3.59|P-value:1.63E-4|Clearance:0.07||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:YDL196W(YDL196W_d)|FD-Score:3.87|P-value:5.54E-5|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YMR290W-A(YMR290W-A_d)|FD-Score:7.96|P-value:8.49E-16|Clearance:2.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPL142C(YPL142C_d)|FD-Score:3.3|P-value:4.84E-4|Clearance:0.03||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2318
Download HOP data (tab-delimited text)  (excel)
Gene:ARO2(YGL148W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:COQ5(YML110C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTS2(YDR371W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:CWC21(YDR482C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:FRA2(YGL220W)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:IRC10(YOL015W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MNT3(YIL014W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MPA43(YNL249C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPP1(YCR026C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OPY1(YBR129C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PPT2(YPL148C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PTC5(YOR090C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RGP1(YDR137W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPS4B(YHR203C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIS2(YKR072C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOL3(YHR163W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TDH3(YGR192C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TED1(YIL039W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:UBP9(YER098W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS61(YDR136C_d)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YGL010W(YGL010W_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YLR271W(YLR271W_p)|FD-Score:10.8|P-value:1.22E-27||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPK1(YKL126W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR197C(YPR197C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZRT3(YKL175W)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency Gene:ARO2(YGL148W)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:BCK1(YJL095W)|FD-Score:3.97|P-value:3.61E-5||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:COQ5(YML110C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes Gene:CTS2(YDR371W_p)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:CWC21(YDR482C)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:FRA2(YGL220W)|FD-Score:3.81|P-value:6.99E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:IRC10(YOL015W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:-3.22|P-value:6.47E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MNT3(YIL014W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Alpha-1,3-mannosyltransferase, adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation Gene:MPA43(YNL249C)|FD-Score:3.32|P-value:4.43E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:NPP1(YCR026C)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:OPY1(YBR129C)|FD-Score:-4.11|P-value:2.01E-5||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PPT2(YPL148C)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PTC5(YOR090C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:RGP1(YDR137W)|FD-Score:-3.11|P-value:9.25E-4||SGD DESC:Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p Gene:RPS4B(YHR203C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SIS2(YKR072C)|FD-Score:-3.12|P-value:8.95E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SOL3(YHR163W)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TDH3(YGR192C)|FD-Score:3.87|P-value:5.47E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication Gene:TED1(YIL039W)|FD-Score:-3.32|P-value:4.46E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:UBP9(YER098W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication Gene:VMA7(YGR020C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS61(YDR136C_d)|FD-Score:-3.35|P-value:3.97E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YGL010W(YGL010W_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YLR271W(YLR271W_p)|FD-Score:10.8|P-value:1.22E-27||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YML007C-A(YML007C-A_p)|FD-Score:3.31|P-value:4.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YPK1(YKL126W)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR197C(YPR197C_d)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPT6(YLR262C)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6 Gene:ZRT3(YKL175W)|FD-Score:-3.16|P-value:7.91E-4||SGD DESC:Vacuolar membrane zinc transporter, transports zinc from storage in the vacuole to the cytoplasm when needed; transcription is induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR290W-A_d7.968.49E-162.01YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YGL055W5.961.30E-91.54OLE1Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
YBL105C4.425.04E-60.41PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YJR123W4.013.08E-50.11RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YBR110W3.904.90E-50.03ALG1Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
YDL196W_d3.875.54E-50.27YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YMR203W3.591.63E-40.07TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YDR047W3.522.14E-40.22HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YPL142C_d3.304.84E-40.03YPL142C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPL33A/YPL143W, a component of the large (60S) ribosomal subunit
YLR197W3.275.31E-40.18NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YDR238C3.099.88E-40.06SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
YOL021C3.030.001210.01DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YOL120C3.020.001260.07RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YKL019W2.950.001580.18RAM2Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
YOR217W2.770.002790.03RFC1Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR271W_p10.801.22E-27YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YKL212W4.415.21E-6SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YER098W4.298.90E-6UBP9Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
YJL095W3.973.61E-5BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YGR192C3.875.47E-5TDH3Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication
YOL015W3.865.65E-5IRC10Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
YGL220W3.816.99E-5FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YGL010W_p3.651.33E-4YGL010W_pPutative protein of unknown function; YGL010W is not an essential gene
YDR371W_p3.571.82E-4CTS2_pPutative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
YKL126W3.492.40E-4YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YML110C3.482.51E-4COQ52-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
YGR020C3.432.97E-4VMA7Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
YPR197C_d3.423.17E-4YPR197C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YCR026C3.354.00E-4NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication
YHR203C3.344.14E-4RPS4BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication

GO enrichment analysis for SGTC_224
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2707.18E-99SGTC_223busulfan 5.0 mMMiscellaneous24780.0892857
0.2656.53E-95SGTC_218alverine citrate 93.8 μMMiscellaneous217180.169492fatty acid desaturase (OLE1)
0.2642.29E-94SGTC_225nsc-122657 1.0 μMMiscellaneous2756850.15942
0.2471.49E-82SGTC_220paclitaxel 373.4 μMMiscellaneous46660.146552
0.1962.02E-52SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.0649351
0.1963.33E-52SGTC_30719117417 49.5 μMChembridge (Drug-like library)455955900.109589fatty acid desaturase (OLE1)
0.1825.83E-45SGTC_31269127922 49.5 μMChembridge (Drug-like library)455956820.144737fatty acid desaturase (OLE1)
0.1791.04E-43SGTC_28879044784 58.4 μMChembridge (Drug-like library)64655460.185714fatty acid desaturase (OLE1)
0.1695.54E-39SGTC_30819117243 49.5 μMChembridge (Drug-like library)455955890.12fatty acid desaturase (OLE1)
0.1647.53E-37SGTC_30549094397 49.5 μMChembridge (Drug-like library)25983540.205882fatty acid desaturase (OLE1)
0.1591.31E-34SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.117647heme biosynthesis & mitochondrial translocase
0.1581.70E-34SGTC_28389002687 71.4 μMChembridge (Drug-like library)64570600.202703fatty acid desaturase (OLE1)
0.1582.44E-34SGTC_7064262-0057 46.9 μMChemDiv (Drug-like library)8832980.104478fatty acid desaturase (OLE1)
0.1547.05E-33SGTC_7211187-1332 279.0 μMChemDiv (Drug-like library)257300.206349cell wall signaling
0.1512.77E-31SGTC_20505263056 171.0 μMChembridge (Fragment library)7634680.119403fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2831799903619.48 μM0.3538462988270Chembridge (Drug-like library)323.309654.03115
SGTC_1235benzylparaben21.5 μM0.3214297180ChemDiv (Drug-like library)228.243283.02613
SGTC_1139k015-002446.9 μM0.317462766652ChemDiv (Drug-like library)305.251313.40205PDR1
SGTC_2845900302442.97 μM0.3076922990173Chembridge (Drug-like library)348.191263.13713amide catabolism
SGTC_1349flufenamic acid69 μM0.3064523371ChemDiv (Drug-like library)281.229913.95226
SGTC_9691313-0238134 μM0.2898555212874ChemDiv (Drug-like library)352.264753.37117mitochondrial processes
SGTC_1463k081-003234.1 μM0.287879977755ChemDiv (Drug-like library)345.114633.15415
SGTC_2839900395125.97 μM0.2878792990634Chembridge (Drug-like library)283.364843.81512
SGTC_413propylparaben100 μM0.2857147175Miscellaneous180.200482.31513amide catabolism
SGTC_150266-008627.1 μM0.2837841712902ChemDiv (Drug-like library)378.50725.23603endomembrane recycling