6690706

3-methyl-N-(pyridin-3-ylmethyl)-1-benzofuran-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2240
Screen concentration 185.9 μM
Source Chembridge (Fragment library)
PubChem CID 805876
SMILES CC1=C(OC2=CC=CC=C12)C(=O)NCC3=CN=CC=C3
Standardized SMILES Cc1c(oc2ccccc12)C(=O)NCc3cccnc3
Molecular weight 266.2946
ALogP 2.55
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11
% growth inhibition (Hom. pool) 10.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 805876
Download HIP data (tab-delimited text)  (excel)
Gene:CDC48(YDL126C)|FD-Score:-4.28|P-value:9.52E-6|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CLP1(YOR250C)|FD-Score:5.58|P-value:1.19E-8|Clearance:0.75||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GLN4(YOR168W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.03||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:IRA1(YBR140C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.21||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:-5.43|P-value:2.89E-8|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MCD1(YDL003W)|FD-Score:4.31|P-value:8.11E-6|Clearance:0.16||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MED8(YBR193C)|FD-Score:5.07|P-value:2.04E-7|Clearance:0.75||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MSL5(YLR116W)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.02||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NDD1(YOR372C)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.01||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:OKP1(YGR179C)|FD-Score:-3.44|P-value:2.92E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PRP42(YDR235W)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.17||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:PRP6(YBR055C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROT1(YMR200W)|FD-Score:-3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPS2(YGL123W)|FD-Score:5.61|P-value:9.87E-9|Clearance:0.75||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC6(YIL068C)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.19||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SUA5(YGL169W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.04||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM40(YMR203W)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:CDC48(YDL126C)|FD-Score:-4.28|P-value:9.52E-6|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CLP1(YOR250C)|FD-Score:5.58|P-value:1.19E-8|Clearance:0.75||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation Gene:GLN4(YOR168W)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.03||SGD DESC:Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions Gene:IRA1(YBR140C)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.21||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:-5.43|P-value:2.89E-8|Clearance:0||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:MCD1(YDL003W)|FD-Score:4.31|P-value:8.11E-6|Clearance:0.16||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:MED8(YBR193C)|FD-Score:5.07|P-value:2.04E-7|Clearance:0.75||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MSL5(YLR116W)|FD-Score:4.15|P-value:1.67E-5|Clearance:0.02||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NDD1(YOR372C)|FD-Score:3.56|P-value:1.82E-4|Clearance:0.01||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:OKP1(YGR179C)|FD-Score:-3.44|P-value:2.92E-4|Clearance:0||SGD DESC:Outer kinetochore protein required for accurate chromosome segregation; component of COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) a kinetochore sub-complex which functions as a platform for kinetochore assembly; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-Q and fission yeast fta7 Gene:PRP42(YDR235W)|FD-Score:3.73|P-value:9.52E-5|Clearance:0.17||SGD DESC:U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats Gene:PRP6(YBR055C)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.4||SGD DESC:Splicing factor, component of the U4/U6-U5 snRNP complex Gene:ROT1(YMR200W)|FD-Score:-3.19|P-value:7.09E-4|Clearance:0||SGD DESC:Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synthesis and in lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation Gene:RPS2(YGL123W)|FD-Score:5.61|P-value:9.87E-9|Clearance:0.75||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:SEC6(YIL068C)|FD-Score:3.14|P-value:8.45E-4|Clearance:0.19||SGD DESC:Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p Gene:SUA5(YGL169W)|FD-Score:3.32|P-value:4.57E-4|Clearance:0.04||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TOM40(YMR203W)|FD-Score:3.28|P-value:5.20E-4|Clearance:0.14||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 805876
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE6(YGR061C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH4(YGL256W)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFT1(YGL071W)|FD-Score:6.41|P-value:7.08E-11||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD17(YNR027W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBF1(YJR060W)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COQ10(YOL008W)|FD-Score:4.84|P-value:6.41E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CTR1(YPR124W)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DLD2(YDL178W)|FD-Score:4.42|P-value:4.82E-6||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EST1(YLR233C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:EST2(YLR318W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAU1(YER183C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FZO1(YBR179C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GLO4(YOR040W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GUF1(YLR289W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HIS5(YIL116W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HPR1(YDR138W)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP26(YBR072W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IRC4(YDR540C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KIP2(YPL155C)|FD-Score:-7.66|P-value:9.09E-15||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LYS12(YIL094C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEF1(YLR069C)|FD-Score:-5.11|P-value:1.61E-7||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHP1(YJL042W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MNN11(YJL183W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:NIP100(YPL174C)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OGG1(YML060W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI7(YDR360W_d)|FD-Score:-4.29|P-value:8.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PBP2(YBR233W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PET100(YDR079W)|FD-Score:-3.89|P-value:5.04E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PET127(YOR017W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PMS1(YNL082W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PTC2(YER089C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:ROY1(YMR258C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL20A(YMR242C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SNF7(YLR025W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNN1(YNL086W_p)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SPO21(YOL091W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPR1(YOR190W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSA4(YER103W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:TDH2(YJR009C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TIF1(YKR059W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOM1(YDR457W)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPN1(YGL186C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:URA10(YMR271C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VPS69(YPR087W_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR219C(YBR219C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL121C(YDL121C_p)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YEF1(YEL041W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL034W(YFL034W_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGR168C(YGR168C_p)|FD-Score:5.83|P-value:2.76E-9||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR124C(YJR124C_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLR283W(YLR283W_p)|FD-Score:-8.44|P-value:1.60E-17||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YMR090W(YMR090W_p)|FD-Score:-5.32|P-value:5.29E-8||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL193W(YNL193W_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL195C(YNL195C_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR097C(YOR097C_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL039W(YPL039W_p)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL168W(YPL168W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YSY6(YBR162W-A)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:ZRG8(YER033C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency Gene:ACB1(YGR037C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:ADE6(YGR061C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Formylglycinamidine-ribonucleotide (FGAM)-synthetase, catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway Gene:ADH4(YGL256W)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Alcohol dehydrogenase isoenzyme type IV, dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency Gene:AFT1(YGL071W)|FD-Score:6.41|P-value:7.08E-11||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:BUD17(YNR027W)|FD-Score:4.11|P-value:1.98E-5||SGD DESC:Putative pyridoxal kinase, a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK) Gene:CBF1(YJR060W)|FD-Score:5.98|P-value:1.10E-9||SGD DESC:Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress Gene:COQ10(YOL008W)|FD-Score:4.84|P-value:6.41E-7||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CTR1(YPR124W)|FD-Score:4.32|P-value:7.66E-6||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DLD2(YDL178W)|FD-Score:4.42|P-value:4.82E-6||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EST1(YLR233C)|FD-Score:-3.64|P-value:1.34E-4||SGD DESC:TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA Gene:EST2(YLR318W)|FD-Score:3.48|P-value:2.50E-4||SGD DESC:Reverse transcriptase subunit of the telomerase holoenzyme, essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia Gene:FAU1(YER183C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FZO1(YBR179C)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system Gene:GLO4(YOR040W)|FD-Score:-3.32|P-value:4.48E-4||SGD DESC:Mitochondrial glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate Gene:GUF1(YLR289W)|FD-Score:-3.25|P-value:5.74E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HIS5(YIL116W)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HPR1(YDR138W)|FD-Score:4.55|P-value:2.70E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HSP26(YBR072W)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity Gene:IRC4(YDR540C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRE1(YHR079C)|FD-Score:-3.34|P-value:4.21E-4||SGD DESC:Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress Gene:KIP2(YPL155C)|FD-Score:-7.66|P-value:9.09E-15||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:LYS12(YIL094C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Homo-isocitrate dehydrogenase, an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate Gene:MAC1(YMR021C)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MEF1(YLR069C)|FD-Score:-5.11|P-value:1.61E-7||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MHP1(YJL042W)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Microtubule-associated protein involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins Gene:MNN11(YJL183W)|FD-Score:-3.36|P-value:3.89E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:NIP100(YPL174C)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued) Gene:OGG1(YML060W)|FD-Score:3.24|P-value:5.98E-4||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OPI7(YDR360W_d)|FD-Score:-4.29|P-value:8.77E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:PBP2(YBR233W)|FD-Score:4.52|P-value:3.14E-6||SGD DESC:RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress Gene:PET100(YDR079W)|FD-Score:-3.89|P-value:5.04E-5||SGD DESC:Chaperone that specifically facilitates the assembly of cytochrome c oxidase, integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme Gene:PET127(YOR017W)|FD-Score:-3.16|P-value:7.88E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PMS1(YNL082W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PTC2(YER089C)|FD-Score:-3.89|P-value:5.08E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:3.82|P-value:6.66E-5||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:ROY1(YMR258C)|FD-Score:4.7|P-value:1.30E-6||SGD DESC:GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p Gene:RPL20A(YMR242C)|FD-Score:3.92|P-value:4.42E-5||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTC3(YHR087W)|FD-Score:-3.11|P-value:9.46E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTT109(YLL002W)|FD-Score:3.44|P-value:2.94E-4||SGD DESC:Histone acetyltransferase critical for cell survival in the presence of DNA damage during S phase; acetylates H3-K56 and H3-K9; involved in non-homologous end joining and in regulation of Ty1 transposition; interacts physically with Vps75p Gene:SNF7(YLR025W)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNN1(YNL086W_p)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes Gene:SPO21(YOL091W)|FD-Score:4.63|P-value:1.79E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPR1(YOR190W)|FD-Score:4.17|P-value:1.52E-5||SGD DESC:Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance Gene:SSA4(YER103W)|FD-Score:3.47|P-value:2.63E-4||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:TDH2(YJR009C)|FD-Score:3.18|P-value:7.36E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TIF1(YKR059W)|FD-Score:3.75|P-value:8.88E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication Gene:TIR3(YIL011W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth Gene:TOM1(YDR457W)|FD-Score:-5.11|P-value:1.64E-7||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TPN1(YGL186C)|FD-Score:5.93|P-value:1.49E-9||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:URA10(YMR271C)|FD-Score:-3.71|P-value:1.05E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VPS69(YPR087W_d)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect Gene:YBR219C(YBR219C_p)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; YBR219C is not an essential gene Gene:YCR075W-A(YCR075W-A_p)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication Gene:YDL121C(YDL121C_p)|FD-Score:-4.48|P-value:3.71E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDL121C is not an essential protein Gene:YEF1(YEL041W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication Gene:YFL034W(YFL034W_p)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk Gene:YGR168C(YGR168C_p)|FD-Score:5.83|P-value:2.76E-9||SGD DESC:Putative protein of unknown function; YGR168C is not an essential gene Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL002W-A(YIL002W-A_p)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJR124C(YJR124C_p)|FD-Score:3.85|P-value:5.87E-5||SGD DESC:Putative protein of unknown function; expression induced under calcium shortage Gene:YLR283W(YLR283W_p)|FD-Score:-8.44|P-value:1.60E-17||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YMR090W(YMR090W_p)|FD-Score:-5.32|P-value:5.29E-8||SGD DESC:Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability Gene:YNL193W(YNL193W_p)|FD-Score:-3.67|P-value:1.20E-4||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YNL195C(YNL195C_p)|FD-Score:4.71|P-value:1.25E-6||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR097C(YOR097C_p)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YPL039W(YPL039W_p)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL168W(YPL168W_p)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated Gene:YSY6(YBR162W-A)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion Gene:ZRG8(YER033C)|FD-Score:-3.2|P-value:6.93E-4||SGD DESC:Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YGL123W5.619.87E-90.75RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YOR250C5.581.19E-80.75CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YBR193C5.072.04E-70.75MED8Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YDL003W4.318.11E-60.16MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YLR116W4.151.67E-50.02MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YBR055C4.131.83E-50.40PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YDR235W3.739.52E-50.17PRP42U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
YOR372C3.561.82E-40.01NDD1Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes
YOR168W3.551.90E-40.03GLN4Glutamine tRNA synthetase, monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Glu); N-terminal domain proposed to be involved in enzyme-tRNA interactions
YBR140C3.522.14E-40.21IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YGL169W3.324.57E-40.04SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YMR203W3.285.20E-40.14TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YIL068C3.148.45E-40.19SEC6Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
YGL150C2.950.001590.01INO80ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription
YOR335C2.940.001640.09ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGL071W6.417.08E-11AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YJR060W5.981.10E-9CBF1Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
YGL186C5.931.49E-9TPN1Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
YGR168C_p5.832.76E-9YGR168C_pPutative protein of unknown function; YGR168C is not an essential gene
YOL008W4.846.41E-7COQ10Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
YNL195C_p4.711.25E-6YNL195C_pPutative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YMR258C4.701.30E-6ROY1GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
YOL091W4.631.79E-6SPO21Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
YDR138W4.552.70E-6HPR1Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
YBR233W4.523.14E-6PBP2RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
YDL178W4.424.82E-6DLD2D-lactate dehydrogenase, located in the mitochondrial matrix
YPR124W4.327.66E-6CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YER183C4.191.37E-5FAU15,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis
YMR021C4.191.41E-5MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YOR190W4.171.52E-5SPR1Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance

GO enrichment analysis for SGTC_2240
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0984.91E-14SGTC_28519015922 71.4 μMChembridge (Drug-like library)29968930.242424
0.0881.53E-11SGTC_7181130-0073 349.0 μMChemDiv (Drug-like library)67400200.126761
0.0841.08E-10SGTC_1165k072-0275 30.3 μMChemDiv (Drug-like library)61535230.25
0.0807.28E-10SGTC_32799140807 49.5 μMChembridge (Drug-like library)455957180.0447761
0.0791.25E-9SGTC_1763st044482 94.5 μMTimTec (Natural product derivative library)885050.0615385copper-dependent oxidative stress
0.0791.28E-9SGTC_22446635614 200.0 μMChembridge (Fragment library)8364990.15873
0.0782.19E-9SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.169014
0.0741.23E-8SGTC_7192810-4230 27.3 μMChemDiv (Drug-like library)7210790.101449
0.0741.32E-8SGTC_575k072-0246 11.9 μMChemDiv (Drug-like library)67967790.155844
0.0741.48E-8SGTC_1569carminic acid 40.6 μMTimTec (Pure natural product library)147490.119048
0.0714.17E-8SGTC_26602',4'-dihydroxychalcone 17.1 μMTimTec (Pure natural product library)53572180.126984
0.0714.65E-8SGTC_13843089-0840 101.0 μMChemDiv (Drug-like library)10698360.0864198
0.0699.58E-8SGTC_2499avocadenofuran 51.5 μMMicrosource (Natural product library)68577920.0952381
0.0699.68E-8SGTC_22567954252 200.0 μMChembridge (Fragment library)29729200.121212mitochondrial processes
0.0691.03E-7SGTC_18845633444 25.0 μMMiscellaneous57242390.205882redox potentiating

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22917917025104.35 μM0.5660382964612Chembridge (Fragment library)280.321143.03813iron homeostasis
SGTC_2279794842332.93 μM0.456141247112Chembridge (Fragment library)260.311583.18314
SGTC_2976900900519.48 μM0.4310342993148Chembridge (Drug-like library)299.390683.28723
SGTC_3291912117771.43 μM0.40909117015612Chembridge (Drug-like library)341.794842.95414
SGTC_20975376186200 μM0.392157742755Chembridge (Fragment library)198.263662.55712
SGTC_2972909361959.92 μM0.3880625236256Chembridge (Drug-like library)300.41853.51513
SGTC_21195528445120.64 μM0.37037791935Chembridge (Fragment library)212.290243.04412
SGTC_22757947463200 μM0.3448281225414Chembridge (Fragment library)216.235880.85813iron homeostasis
SGTC_3351915031221.6 μM0.3333334904905Chembridge (Drug-like library)329.398363.17114cell wall
SGTC_3082911700849.47 μM0.32203441448529Chembridge (Drug-like library)240.300342.44112