6635614

N-(2,3-dichlorophenyl)-2-methoxyacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2244
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 836499
SMILES COCC(=O)NC1=C(C(=CC=C1)Cl)Cl
Standardized SMILES COCC(=O)Nc1cccc(Cl)c1Cl
Molecular weight 234.0793
ALogP 2.02
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 5.97
% growth inhibition (Hom. pool) 6.15


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 836499
Download HIP data (tab-delimited text)  (excel)
Gene:AME1(YBR211C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.15||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP7(YPR034W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:FAS1(YKL182W)|FD-Score:4.46|P-value:4.14E-6|Clearance:0.77||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IMP3(YHR148W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.11||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LST8(YNL006W)|FD-Score:-3.25|P-value:5.80E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MRD1(YPR112C)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.77||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:UTP8(YGR128C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.19||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:AME1(YBR211C)|FD-Score:3.44|P-value:2.91E-4|Clearance:0.15||SGD DESC:Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress Gene:ARP7(YPR034W)|FD-Score:-3.63|P-value:1.42E-4|Clearance:0||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:FAS1(YKL182W)|FD-Score:4.46|P-value:4.14E-6|Clearance:0.77||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:IMP3(YHR148W)|FD-Score:3.1|P-value:9.75E-4|Clearance:0.11||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:LST8(YNL006W)|FD-Score:-3.25|P-value:5.80E-4|Clearance:0||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:MRD1(YPR112C)|FD-Score:4.21|P-value:1.26E-5|Clearance:0.77||SGD DESC:Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region Gene:UTP8(YGR128C)|FD-Score:3.29|P-value:5.06E-4|Clearance:0.19||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 836499
Download HOP data (tab-delimited text)  (excel)
Gene:AAH1(YNL141W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APP1(YNL094W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:BLS1(YLR408C_p)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BOP2(YLR267W_p)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Protein of unknown function Gene:CNM67(YNL225C)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:ECM30(YLR436C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ECM38(YLR299W)|FD-Score:-4.48|P-value:3.79E-6||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:ECM4(YKR076W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ECM8(YBR076W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Non-essential protein of unknown function Gene:FMP16(YDR070C_p)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:ICL1(YER065C)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:MNL1(YHR204W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNN4(YKL201C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRPS9(YBR146W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NSG1(YHR133C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OMS1(YDR316W)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PGM3(YMR278W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIG2(YIL045W)|FD-Score:-5.88|P-value:1.99E-9||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PTC2(YER089C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RNH1(YMR234W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:SGE1(YPR198W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIW14(YNL032W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SMY1(YKL079W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SUT2(YPR009W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAZ1(YPR140W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:UBP12(YJL197W)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAB2(YEL005C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBR238C(YBR238C)|FD-Score:-4.44|P-value:4.47E-6||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCT1(YLL055W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR445C(YDR445C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL235W(YGL235W_p)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL144W(YJL144W)|FD-Score:-3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR038C(YJR038C_d)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR001C(YLR001C_p)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR283W(YLR283W_p)|FD-Score:-6.68|P-value:1.17E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL194C(YNL194C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YNL195C(YNL195C_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL067C(YPL067C_p)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL077C(YPL077C_p)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL109C(YPL109C_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZRC1(YMR243C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:AAH1(YNL141W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APP1(YNL094W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway Gene:BLS1(YLR408C_p)|FD-Score:-4.3|P-value:8.68E-6||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BOP2(YLR267W_p)|FD-Score:-3.8|P-value:7.32E-5||SGD DESC:Protein of unknown function Gene:CNM67(YNL225C)|FD-Score:-4.02|P-value:2.93E-5||SGD DESC:Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication Gene:ECM30(YLR436C)|FD-Score:5.57|P-value:1.25E-8||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ECM38(YLR299W)|FD-Score:-4.48|P-value:3.79E-6||SGD DESC:Gamma-glutamyltranspeptidase, major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation Gene:ECM4(YKR076W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:ECM8(YBR076W)|FD-Score:3.46|P-value:2.75E-4||SGD DESC:Non-essential protein of unknown function Gene:FMP16(YDR070C_p)|FD-Score:3.12|P-value:9.08E-4||SGD DESC:Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress Gene:GLY1(YEL046C)|FD-Score:-3.33|P-value:4.28E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:ICL1(YER065C)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Isocitrate lyase, catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose Gene:MNL1(YHR204W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MNN4(YKL201C)|FD-Score:3.87|P-value:5.43E-5||SGD DESC:Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication Gene:MRPS9(YBR146W)|FD-Score:3.15|P-value:8.12E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:-3.14|P-value:8.32E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:NSG1(YHR133C)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OMS1(YDR316W)|FD-Score:4.37|P-value:6.20E-6||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PGM3(YMR278W)|FD-Score:-3.42|P-value:3.18E-4||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIG2(YIL045W)|FD-Score:-5.88|P-value:1.99E-9||SGD DESC:Putative type-1 protein phosphatase targeting subunit that tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase Gene:PTC2(YER089C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication Gene:QDR2(YIL121W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RNH1(YMR234W)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201) Gene:SGE1(YPR198W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIW14(YNL032W)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm Gene:SMY1(YKL079W)|FD-Score:-3.23|P-value:6.19E-4||SGD DESC:Protein that interacts with Myo2p, proposed to be involved in exocytosis; N-terminal domain is related to the motor domain of kinesins Gene:SUT2(YPR009W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p Gene:TAZ1(YPR140W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome Gene:UBP12(YJL197W)|FD-Score:-3.9|P-value:4.83E-5||SGD DESC:Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm Gene:VAB2(YEL005C)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBR238C(YBR238C)|FD-Score:-4.44|P-value:4.47E-6||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YCT1(YLL055W)|FD-Score:-3.66|P-value:1.24E-4||SGD DESC:High-affinity cysteine-specific transporter with similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene Gene:YDR445C(YDR445C_d)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGL235W(YGL235W_p)|FD-Score:-3.23|P-value:6.10E-4||SGD DESC:Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2 Gene:YJL144W(YJL144W)|FD-Score:-3.93|P-value:4.29E-5||SGD DESC:Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress Gene:YJR038C(YJR038C_d)|FD-Score:-4.26|P-value:1.00E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL162C(YKL162C_p)|FD-Score:-4.06|P-value:2.46E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:YLR001C(YLR001C_p)|FD-Score:-3.13|P-value:8.78E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated Gene:YLR283W(YLR283W_p)|FD-Score:-6.68|P-value:1.17E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR283W is not an essential gene Gene:YNL050C(YNL050C_p)|FD-Score:4.13|P-value:1.80E-5||SGD DESC:Putative protein of unknown function; YNL050c is not an essential gene Gene:YNL194C(YNL194C)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Integral membrane protein required for sporulation and plasma membrane sphingolipid content; has sequence similarity to SUR7 and FMP45; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate Gene:YNL195C(YNL195C_p)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPL067C(YPL067C_p)|FD-Score:-3.71|P-value:1.06E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene Gene:YPL073C(YPL073C_d)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments Gene:YPL077C(YPL077C_p)|FD-Score:-4.66|P-value:1.58E-6||SGD DESC:Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication Gene:YPL109C(YPL109C_p)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ZRC1(YMR243C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL182W4.464.14E-60.77FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YPR112C4.211.26E-50.77MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YBR211C3.442.91E-40.15AME1Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
YGR128C3.295.06E-40.19UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YHR148W3.109.75E-40.11IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YJR045C2.990.001410.01SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YLR355C2.980.001460.18ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YDL166C2.800.002590.17FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
YJL143W2.620.004380.09TIM17Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
YBR055C2.530.005720.06PRP6Splicing factor, component of the U4/U6-U5 snRNP complex
YDR361C2.470.006790.06BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDL108W2.410.008070.03KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YML126C2.380.008670.04ERG133-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
YDR188W2.340.009650.00CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YBR142W2.340.009760.00MAK5Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR436C5.571.25E-8ECM30Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
YDR316W4.376.20E-6OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YNL094W4.201.33E-5APP1Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
YNL050C_p4.131.80E-5YNL050C_pPutative protein of unknown function; YNL050c is not an essential gene
YKL201C3.875.43E-5MNN4Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
YMR234W3.651.30E-4RNH1Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I; the homolog of mammalian RNAse HII (the S. cerevisiae homolog of mammalian RNAse HI is RNH201)
YNL141W3.591.65E-4AAH1Adenine deaminase (adenine aminohydrolase), converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
YPR198W3.581.71E-4SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
YPR009W3.571.79E-4SUT2Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
YBR076W3.462.75E-4ECM8Non-essential protein of unknown function
YGL071W3.295.08E-4AFT1Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
YEL005C3.245.95E-4VAB2Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter
YDR445C_d3.226.41E-4YDR445C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPR140W3.216.58E-4TAZ1Lyso-phosphatidylcholine acyltransferase, required for normal phospholipid content of mitochondrial membranes; may remodel acyl groups of cardiolipin in the inner membrane; human ortholog tafazzin is implicated in Barth syndrome
YMR243C3.168.02E-4ZRC1Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2244
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1288.12E-23SGTC_2261,3-diallylurea 8.3 mMMiscellaneous745370.119048
0.0849.56E-11SGTC_22456637752 200.0 μMChembridge (Fragment library)6818900.526316
0.0791.28E-9SGTC_22406690706 186.0 μMChembridge (Fragment library)8058760.15873
0.0722.46E-8SGTC_1066ketoconazole 628.0 nMNIH Clinical Collection4562010.134831
0.0691.03E-7SGTC_2417cerulenin 830.0 nMICCB bioactive library52820540.0862069
0.0595.60E-6SGTC_31679103034 49.5 μMChembridge (Drug-like library)51278590.232143RPP1 & pyrimidine depletion
0.0571.25E-5SGTC_15983(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin 19.1 μMTimTec (Pure natural product library)63129470.15
0.0561.62E-5SGTC_1682st012968 530.0 nMTimTec (Natural product derivative library)242072700.0806452
0.0491.44E-4SGTC_8770771-0151 33.1 μMChemDiv (Drug-like library)61665230.0769231
0.0491.63E-4SGTC_23437990716 14.8 μMChembridge (Fragment library)64568620.0833333
0.0482.05E-4SGTC_31509097574 49.5 μMChembridge (Drug-like library)172174760.149254
0.0472.81E-4SGTC_1920st075728 45.7 μMTimTec (Natural product derivative library)34003060.123288
0.0463.81E-4SGTC_2727riluzole 51.8 μMMiscellaneous50700.0689655mitochondrial processes
0.0446.28E-4SGTC_31769106582 49.5 μMChembridge (Drug-like library)170180270.277778
0.0430.00104SGTC_687k029-0044 34.9 μMChemDiv (Drug-like library)58293380.101449PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22436634756200 μM0.589744885676Chembridge (Fragment library)234.079262.01512
SGTC_22456637752200 μM0.526316681890Chembridge (Fragment library)199.63421.35112
SGTC_1300136-0219337.66 μM0.488372234244ChemDiv (Drug-like library)296.148643.77812
SGTC_74000s-0487160.28 μM0.466667677548ChemDiv (Drug-like library)277.702982.87223
SGTC_20945332512191.67 μM0.44186755774Chembridge (Fragment library)215.247821.59512
SGTC_2822799471871.43 μM0.4181821018011Chembridge (Drug-like library)373.876463.58214
SGTC_7924534-1957155 μM0.3653851717904ChemDiv (Drug-like library)282.337022.73412
SGTC_2895905444758.44 μM0.362697450Chembridge (Drug-like library)343.850483.16113
SGTC_23649055074200 μM0.3529416470630Chembridge (Fragment library)275.733462.52922
SGTC_2994905352071.43 μM0.3529414647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion