7293507

N-[4-(2,5-dioxopyrrol-1-yl)-2-methylphenyl]acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2246
Screen concentration 91.6 μM
Source Chembridge (Fragment library)
PubChem CID 887656
SMILES CC1=C(C=CC(=C1)N2C(=O)C=CC2=O)NC(=O)C
Standardized SMILES CC(=O)Nc1ccc(cc1C)N2C(=O)C=CC2=O
Molecular weight 244.246
ALogP 0.99
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.32
% growth inhibition (Hom. pool) 14.54


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 887656
Download HIP data (tab-delimited text)  (excel)
Gene:BRN1(YBL097W)|FD-Score:5.31|P-value:5.37E-8|Clearance:0.02||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DBP2(YNL112W)|FD-Score:5.03|P-value:2.49E-7|Clearance:0.05||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG1(YGR175C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG26(YGL001C)|FD-Score:3.77|P-value:8.00E-5|Clearance:0.06||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ESF1(YDR365C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.07||SGD DESC:Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels Gene:GPN3(YLR243W_p)|FD-Score:5.3|P-value:5.90E-8|Clearance:0.01||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:GRS1(YBR121C)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HSP10(YOR020C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.09||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:HTS1(YPR033C)|FD-Score:3.38|P-value:3.65E-4|Clearance:0.1||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:HYP2(YEL034W)|FD-Score:5.78|P-value:3.72E-9|Clearance:0.47||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.01||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LCB1(YMR296C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.19||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LST8(YNL006W)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.04||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:NIP1(YMR309C)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP1(YDL014W)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:-3.9|P-value:4.76E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NSE5(YML023C)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RBA50(YDR527W)|FD-Score:13.2|P-value:2.31E-40|Clearance:4.56||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:8.68|P-value:1.90E-18|Clearance:2.9||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN5(YDL147W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO26(YPR187W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRN6(YBL014C)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.02||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSM10(YDR041W)|FD-Score:4.98|P-value:3.15E-7|Clearance:0.64||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:RTP1(YMR185W_p)|FD-Score:4.34|P-value:7.19E-6|Clearance:0.51||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:TRS23(YDR246W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.02||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:UTP22(YGR090W)|FD-Score:5.28|P-value:6.36E-8|Clearance:0.26||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YBL077W(YBL077W_d)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ZPR1(YGR211W)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.02||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress Gene:BRN1(YBL097W)|FD-Score:5.31|P-value:5.37E-8|Clearance:0.02||SGD DESC:Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission Gene:DBP2(YNL112W)|FD-Score:5.03|P-value:2.49E-7|Clearance:0.05||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG1(YGR175C)|FD-Score:3.28|P-value:5.26E-4|Clearance:0.01||SGD DESC:Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine Gene:ERG26(YGL001C)|FD-Score:3.77|P-value:8.00E-5|Clearance:0.06||SGD DESC:C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis Gene:ESF1(YDR365C)|FD-Score:3.19|P-value:7.01E-4|Clearance:0.07||SGD DESC:Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels Gene:GPN3(YLR243W_p)|FD-Score:5.3|P-value:5.90E-8|Clearance:0.01||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p Gene:GRS1(YBR121C)|FD-Score:-4.2|P-value:1.33E-5|Clearance:0||SGD DESC:Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication Gene:HSP10(YOR020C)|FD-Score:3.51|P-value:2.23E-4|Clearance:0.09||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:HTS1(YPR033C)|FD-Score:3.38|P-value:3.65E-4|Clearance:0.1||SGD DESC:Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome Gene:HYP2(YEL034W)|FD-Score:5.78|P-value:3.72E-9|Clearance:0.47||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:ISD11(YER048W-A)|FD-Score:3.26|P-value:5.54E-4|Clearance:0.01||SGD DESC:Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis Gene:LCB1(YMR296C)|FD-Score:3.12|P-value:9.01E-4|Clearance:0.19||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:LST8(YNL006W)|FD-Score:3.81|P-value:6.94E-5|Clearance:0.04||SGD DESC:Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat Gene:NIP1(YMR309C)|FD-Score:-4.26|P-value:1.04E-5|Clearance:0||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NOP1(YDL014W)|FD-Score:-3.3|P-value:4.82E-4|Clearance:0||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:NOP2(YNL061W)|FD-Score:-3.9|P-value:4.76E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NSE5(YML023C)|FD-Score:3.39|P-value:3.51E-4|Clearance:0.01||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RBA50(YDR527W)|FD-Score:13.2|P-value:2.31E-40|Clearance:4.56||SGD DESC:Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1 Gene:RET1(YOR207C)|FD-Score:8.68|P-value:1.90E-18|Clearance:2.9||SGD DESC:Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs Gene:RPN5(YDL147W)|FD-Score:3.58|P-value:1.71E-4|Clearance:0.07||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPO26(YPR187W)|FD-Score:3.25|P-value:5.68E-4|Clearance:0.06||SGD DESC:RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit Gene:RRN6(YBL014C)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.02||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RSM10(YDR041W)|FD-Score:4.98|P-value:3.15E-7|Clearance:0.64||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins Gene:RTP1(YMR185W_p)|FD-Score:4.34|P-value:7.19E-6|Clearance:0.51||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:TRS23(YDR246W)|FD-Score:3.42|P-value:3.08E-4|Clearance:0.02||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4 Gene:UTP22(YGR090W)|FD-Score:5.28|P-value:6.36E-8|Clearance:0.26||SGD DESC:Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals Gene:YBL077W(YBL077W_d)|FD-Score:3.72|P-value:1.02E-4|Clearance:0.13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C Gene:ZPR1(YGR211W)|FD-Score:3.83|P-value:6.36E-5|Clearance:0.02||SGD DESC:Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 887656
Download HOP data (tab-delimited text)  (excel)
Gene:ALF1(YNL148C)|FD-Score:18.6|P-value:8.10E-78||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARC18(YLR370C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG21(YPL100W)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BIO4(YNR057C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BLS1(YLR408C_p)|FD-Score:4.94|P-value:3.82E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BTS1(YPL069C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCS1(YMR038C)|FD-Score:6.27|P-value:1.84E-10||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:12.4|P-value:2.27E-35||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CPR7(YJR032W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CYK3(YDL117W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:CYS3(YAL012W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM30(YLR436C)|FD-Score:-4.29|P-value:9.13E-6||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ERG2(YMR202W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:9.42|P-value:2.27E-21||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP40(YPL222W_p)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HOM6(YJR139C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPR1(YDR138W)|FD-Score:-4.33|P-value:7.30E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IMD3(YLR432W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IMP2'(YIL154C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC4(YDR540C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JJJ3(YJR097W)|FD-Score:7.07|P-value:7.90E-13||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KIP2(YPL155C)|FD-Score:5.23|P-value:8.70E-8||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:KTR2(YKR061W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:9.96|P-value:1.14E-23||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:6.03|P-value:8.02E-10||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAD3(YJL013C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MCM16(YPR046W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MET5(YJR137C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MNL1(YHR204W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOG1(YJR074W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL20(YKR085C)|FD-Score:-4.47|P-value:3.82E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOP13(YNL175C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NSR1(YGR159C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OPI7(YDR360W_d)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OPI9(YLR338W_d)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OYE2(YHR179W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDC5(YLR134W)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEA2(YER149C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHO90(YJL198W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PHR1(YOR386W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:POL32(YJR043C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:7.81|P-value:2.81E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUB1(YNL016W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:PUS5(YLR165C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD24(YER173W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RCR2(YDR003W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RPL13A(YDL082W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.85|P-value:4.51E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTG2(YGL252C)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTR1(YER139C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:5.44|P-value:2.66E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SET2(YJL168C)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SMK1(YPR054W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPS19(YNL202W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:STP1(YDR463W)|FD-Score:-5.84|P-value:2.59E-9||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP3(YLR375W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SVP26(YHR181W)|FD-Score:5.39|P-value:3.52E-8||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWH1(YAR042W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWR1(YDR334W)|FD-Score:8.28|P-value:6.08E-17||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIR1(YER011W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:7.12|P-value:5.24E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP2(YOR124C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VIP1(YLR410W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VTA1(YLR181C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAR047C(YAR047C_d)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR225W(YBR225W_p)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR049C(YCR049C_d)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR080C(YHR080C)|FD-Score:-4.58|P-value:2.27E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIP5(YGL161C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL193W(YJL193W_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR056C(YJR056C_p)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR115W(YJR115W_p)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YLR290C(YLR290C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL163W(YOL163W_p)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR029W(YOR029W_d)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4 Gene:ALF1(YNL148C)|FD-Score:18.6|P-value:8.10E-78||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARC18(YLR370C)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG21(YPL100W)|FD-Score:3.82|P-value:6.74E-5||SGD DESC:Phosphoinositide binding protein required for vesicle formation in the cytoplasm-to-vacuole targeting (Cvt) pathway; binds both phosphatidylinositol (3,5)-bisphosphate and phosphatidylinositol 3-phosphate; WD-40 repeat protein Gene:BIO4(YNR057C)|FD-Score:-3.78|P-value:7.72E-5||SGD DESC:Dethiobiotin synthetase, catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; expression appears to be repressed at low iron levels Gene:BLS1(YLR408C_p)|FD-Score:4.94|P-value:3.82E-7||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BTS1(YPL069C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CCS1(YMR038C)|FD-Score:6.27|P-value:1.84E-10||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CIN1(YOR349W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:12.4|P-value:2.27E-35||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CLA4(YNL298W)|FD-Score:3.77|P-value:8.07E-5||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p Gene:CPR7(YJR032W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity Gene:CYK3(YDL117W)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:CYS3(YAL012W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-3.2|P-value:6.90E-4||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DYN3(YMR299C)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration Gene:ECM30(YLR436C)|FD-Score:-4.29|P-value:9.13E-6||SGD DESC:Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm Gene:ERG2(YMR202W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:ERG4(YGL012W)|FD-Score:9.42|P-value:2.27E-21||SGD DESC:C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol Gene:FMP40(YPL222W_p)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Putative protein of unknown function; proposed to be involved in responding to environmental stresses; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:HOM6(YJR139C)|FD-Score:4.39|P-value:5.67E-6||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HPR1(YDR138W)|FD-Score:-4.33|P-value:7.30E-6||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:IMD3(YLR432W)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IMP2'(YIL154C)|FD-Score:-3.28|P-value:5.17E-4||SGD DESC:Transcriptional activator involved in maintenance of ion homeostasis and protection against DNA damage caused by bleomycin and other oxidants, contains a C-terminal leucine-rich repeat Gene:IRC4(YDR540C)|FD-Score:3.4|P-value:3.33E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:JJJ3(YJR097W)|FD-Score:7.07|P-value:7.90E-13||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KIP2(YPL155C)|FD-Score:5.23|P-value:8.70E-8||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:KTR2(YKR061W)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication Gene:LTE1(YAL024C)|FD-Score:9.96|P-value:1.14E-23||SGD DESC:Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures Gene:MAC1(YMR021C)|FD-Score:6.03|P-value:8.02E-10||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MAD3(YJL013C)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Subunit of the spindle-assembly checkpoint complex, which delays anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover Gene:MCM16(YPR046W)|FD-Score:-3.1|P-value:9.82E-4||SGD DESC:Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3 Gene:MET5(YJR137C)|FD-Score:4.57|P-value:2.38E-6||SGD DESC:Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine Gene:MNL1(YHR204W)|FD-Score:3.1|P-value:9.62E-4||SGD DESC:Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; in complex with Pdi1p, generates a Man7GlcNac2 oligosaccharide signal on glycoproteins destined for ubiquitin-proteasome degradation Gene:MOG1(YJR074W)|FD-Score:3.26|P-value:5.66E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL20(YKR085C)|FD-Score:-4.47|P-value:3.82E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NOP13(YNL175C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NSR1(YGR159C)|FD-Score:-4.04|P-value:2.70E-5||SGD DESC:Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis Gene:OPI7(YDR360W_d)|FD-Score:4.33|P-value:7.62E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C. Gene:OPI9(YLR338W_d)|FD-Score:5.57|P-value:1.26E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OYE2(YHR179W)|FD-Score:3.77|P-value:8.14E-5||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PDC5(YLR134W)|FD-Score:-3.93|P-value:4.25E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PEA2(YER149C)|FD-Score:3.24|P-value:6.01E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHO90(YJL198W)|FD-Score:-3.14|P-value:8.53E-4||SGD DESC:Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication Gene:PHR1(YOR386W)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:DNA photolyase involved in photoreactivation, repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p Gene:POL32(YJR043C)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p Gene:PRO2(YOR323C)|FD-Score:7.81|P-value:2.81E-15||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PUB1(YNL016W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress Gene:PUS5(YLR165C)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability Gene:RAD24(YER173W)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein Gene:RCR2(YDR003W)|FD-Score:3.24|P-value:5.99E-4||SGD DESC:Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication Gene:RPL13A(YDL082W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.55|P-value:1.90E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:8.85|P-value:4.51E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTG2(YGL252C)|FD-Score:3.1|P-value:9.67E-4||SGD DESC:Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p Gene:RTR1(YER139C)|FD-Score:3.35|P-value:4.11E-4||SGD DESC:CTD phosphatase; dephosphorylates S5-P in the C-terminal domain of Rpo21p; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication Gene:SAC1(YKL212W)|FD-Score:5.44|P-value:2.66E-8||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAC6(YDR129C)|FD-Score:4.6|P-value:2.14E-6||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SET2(YJL168C)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SMK1(YPR054W)|FD-Score:3.19|P-value:7.14E-4||SGD DESC:Middle sporulation-specific mitogen-activated protein kinase (MAPK) required for production of the outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p Gene:SPS19(YNL202W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Peroxisomal 2,4-dienoyl-CoA reductase, auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate Gene:STP1(YDR463W)|FD-Score:-5.84|P-value:2.59E-9||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:STP3(YLR375W)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Zinc-finger protein of unknown function, possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids Gene:SVP26(YHR181W)|FD-Score:5.39|P-value:3.52E-8||SGD DESC:Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment Gene:SWH1(YAR042W)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction Gene:SWR1(YDR334W)|FD-Score:8.28|P-value:6.08E-17||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TAT1(YBR069C)|FD-Score:-3.22|P-value:6.45E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TIR1(YER011W)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TSC3(YBR058C-A)|FD-Score:7.12|P-value:5.24E-13||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUB3(YML124C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:UBP2(YOR124C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; interacts with Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity Gene:VIP1(YLR410W)|FD-Score:3.19|P-value:7.09E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; IP7 production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1 Gene:VTA1(YLR181C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YAR047C(YAR047C_d)|FD-Score:5.09|P-value:1.80E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR225W(YBR225W_p)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.94|P-value:4.06E-5||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR049C(YCR049C_d)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.37|P-value:6.19E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR080C(YHR080C)|FD-Score:-4.58|P-value:2.27E-6||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YHR080C has a paralog, YSP2, that arose from the whole genome duplication Gene:YIP5(YGL161C)|FD-Score:3.17|P-value:7.64E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL193W(YJL193W_p)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect Gene:YJR056C(YJR056C_p)|FD-Score:-3.22|P-value:6.31E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YJR115W(YJR115W_p)|FD-Score:3.79|P-value:7.55E-5||SGD DESC:Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication Gene:YLR290C(YLR290C_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YMR294W-A(YMR294W-A_d)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.92|P-value:4.37E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL163W(YOL163W_p)|FD-Score:3.27|P-value:5.35E-4||SGD DESC:Putative protein of unknown function; member of the Dal5p subfamily of the major facilitator family Gene:YOR029W(YOR029W_d)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:3.5|P-value:2.36E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL014W(YPL014W_p)|FD-Score:3.46|P-value:2.70E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus Gene:YPL102C(YPL102C_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR527W13.202.31E-404.56RBA50Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
YOR207C8.681.90E-182.90RET1Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
YEL034W5.783.72E-90.47HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YBL097W5.315.37E-80.02BRN1Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
YLR243W_p5.305.90E-80.01GPN3_pPutative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
YGR090W5.286.36E-80.26UTP22Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
YNL112W5.032.49E-70.05DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YDR041W4.983.15E-70.64RSM10Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
YMR185W_p4.347.19E-60.51RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YGR211W3.836.36E-50.02ZPR1Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
YNL006W3.816.94E-50.04LST8Protein required for the transport of amino acid permease Gap1p from the Golgi to the cell surface; component of the TOR signaling pathway; associates with both Tor1p and Tor2p; contains a WD-repeat
YGL001C3.778.00E-50.06ERG26C-3 sterol dehydrogenase, catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis
YBL077W_d3.721.02E-40.13YBL077W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C
YDL147W3.581.71E-40.07RPN5Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein
YOR020C3.512.23E-40.09HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL148C18.608.10E-78ALF1Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
YPL241C12.402.27E-35CIN2GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
YAL024C9.961.14E-23LTE1Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
YGL012W9.422.27E-21ERG4C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
YDL020C8.854.51E-19RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YDR334W8.286.08E-17SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YOR323C7.812.81E-15PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YBR058C-A7.125.24E-13TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJR097W7.077.90E-13JJJ3Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
YMR038C6.271.84E-10CCS1Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress
YMR021C6.038.02E-10MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YLR338W_d5.571.26E-8OPI9_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
YER007W5.492.03E-8PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YKL212W5.442.66E-8SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YHR181W5.393.52E-8SVP26Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment

GO enrichment analysis for SGTC_2246
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3861.41E-207SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.173913
0.3541.37E-173SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.185185
0.3162.73E-137SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0972222
0.3131.56E-134SGTC_32829108498 15.5 μMChembridge (Drug-like library)164596450.179104
0.3062.55E-128SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.175439
0.2873.48E-112SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.196078tubulin folding & SWR complex
0.2861.69E-111SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.181818
0.2727.17E-101SGTC_20044023728 41.9 μMChembridge (Fragment library)3600840.0784314
0.2722.42E-100SGTC_23236148992 148.3 μMChembridge (Fragment library)28882200.192982
0.2584.21E-90SGTC_24305571206 200.0 μMMiscellaneous17317710.0882353
0.2416.15E-79SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.125tubulin folding & SWR complex
0.2338.43E-74SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.103448TSC3-RPN4
0.2261.80E-69SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.08
0.2232.65E-67SGTC_2510benzyl isothiocyanate 8.3 μMMicrosource (Natural product library)23460.0612245
0.2171.48E-63SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0689655

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_12220366-00741.18 μM0.377778286520ChemDiv (Drug-like library)245.230741.59204TSC3-RPN4
SGTC_22077258803200 μM0.3555563666799Chembridge (Fragment library)244.08521.68212
SGTC_213453248361.46 μM0.318182699272Chembridge (Fragment library)258.272561.23804
SGTC_215856571233.62 μM0.31252858827Chembridge (Fragment library)269.29521.81603
SGTC_21405586273200 μM0.311111854421Chembridge (Fragment library)223.65562.1402RSC complex & mRNA processing
SGTC_22817943571200 μM0.3018871244024Chembridge (Fragment library)231.247222.47913
SGTC_22957971645200 μM0.2978722978226Chembridge (Fragment library)204.268242.09911
SGTC_2838900268771.43 μM0.2931036457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_29087930301100 μM0.2888892223178Chembridge (Drug-like library)387.431143.00533
SGTC_3164910070549.47 μM0.28070217174235Chembridge (Drug-like library)299.321182.8714