cerulenin

(2R,3S)-3-[(4E,7E)-nona-4,7-dienoyl]oxirane-2-carboxamide


Established Yeast Drug Target : FAS1

Cerulenin, an antibiotic and antifungal, is an inhibitor of fatty acid biosynthesis.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Antifungal Agents;Fatty Acid Synthesis Inhibitors



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2247
Screen concentration 830.0 nM
Source ICCB bioactive library
PubChem CID 5282054
SMILES CC=CCC=CCCC(=O)C1C(O1)C(=O)N
Standardized SMILES CC=CCC=CCCC(=O)C1OC1C(=O)N
Molecular weight 223.2683
ALogP 1.09
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.13
% growth inhibition (Hom. pool) -1.39


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5282054
Download HIP data (tab-delimited text)  (excel)
Gene:ARP3(YJR065C)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ASK1(YKL052C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:BRX1(YOL077C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.22||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC12(YHR107C)|FD-Score:7.41|P-value:6.54E-14|Clearance:2.9||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CTR86(YCR054C)|FD-Score:4.51|P-value:3.28E-6|Clearance:0.16||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:ESF1(YDR365C)|FD-Score:-4.97|P-value:3.40E-7|Clearance:0||SGD DESC:Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels Gene:GPI1(YGR216C)|FD-Score:-3.99|P-value:3.33E-5|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:HEM2(YGL040C)|FD-Score:3.11|P-value:9.48E-4|Clearance:0.15||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HSH155(YMR288W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.12||SGD DESC:U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 Gene:HSP10(YOR020C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.08||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:NOP1(YDL014W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POL5(YEL055C)|FD-Score:-4.08|P-value:2.26E-5|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:RPB3(YIL021W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.1||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.33||SGD DESC:RNA polymerase III subunit C53 Gene:RPL30(YGL030W)|FD-Score:3.8|P-value:7.34E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:SDH3(YKL141W)|FD-Score:-4.35|P-value:6.95E-6|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC61(YLR378C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SPC3(YLR066W)|FD-Score:-4.21|P-value:1.29E-5|Clearance:0||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SRP54(YPR088C)|FD-Score:-3.14|P-value:8.31E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TAF6(YGL112C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIM10(YHR005C-A)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.07||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YBR089W(YBR089W_d)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YML6(YML025C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YPL251W(YPL251W_d)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C Gene:ARP3(YJR065C)|FD-Score:3.13|P-value:8.73E-4|Clearance:0.02||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:ASK1(YKL052C)|FD-Score:-3.98|P-value:3.47E-5|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress Gene:BRX1(YOL077C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.22||SGD DESC:Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Gene:CDC12(YHR107C)|FD-Score:7.41|P-value:6.54E-14|Clearance:2.9||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CTR86(YCR054C)|FD-Score:4.51|P-value:3.28E-6|Clearance:0.16||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:ESF1(YDR365C)|FD-Score:-4.97|P-value:3.40E-7|Clearance:0||SGD DESC:Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels Gene:GPI1(YGR216C)|FD-Score:-3.99|P-value:3.33E-5|Clearance:0||SGD DESC:Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs Gene:HEM2(YGL040C)|FD-Score:3.11|P-value:9.48E-4|Clearance:0.15||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HSH155(YMR288W)|FD-Score:3.92|P-value:4.47E-5|Clearance:0.12||SGD DESC:U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155 Gene:HSP10(YOR020C)|FD-Score:3.21|P-value:6.74E-4|Clearance:0.08||SGD DESC:Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES Gene:NOP1(YDL014W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.1||SGD DESC:Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin Gene:POL5(YEL055C)|FD-Score:-4.08|P-value:2.26E-5|Clearance:0||SGD DESC:DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA Gene:RPB3(YIL021W)|FD-Score:3.3|P-value:4.79E-4|Clearance:0.1||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:4.35|P-value:6.84E-6|Clearance:0.33||SGD DESC:RNA polymerase III subunit C53 Gene:RPL30(YGL030W)|FD-Score:3.8|P-value:7.34E-5|Clearance:0.14||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:SDH3(YKL141W)|FD-Score:-4.35|P-value:6.95E-6|Clearance:0||SGD DESC:Subunit of both succinate dehydrogenase and of TIM22 translocase; functions as the cytochrome b subunit of succinate dehydrogenase, which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex Gene:SEC61(YLR378C)|FD-Score:-3.79|P-value:7.66E-5|Clearance:0||SGD DESC:Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER Gene:SPC3(YLR066W)|FD-Score:-4.21|P-value:1.29E-5|Clearance:0||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23 Gene:SRP54(YPR088C)|FD-Score:-3.14|P-value:8.31E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain Gene:TAF6(YGL112C)|FD-Score:-3.18|P-value:7.46E-4|Clearance:0||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TIM10(YHR005C-A)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.07||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:YBR089W(YBR089W_d)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30 Gene:YML6(YML025C)|FD-Score:-3.79|P-value:7.44E-5|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins Gene:YPL251W(YPL251W_d)|FD-Score:-3.47|P-value:2.63E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5282054
Download HOP data (tab-delimited text)  (excel)
Gene:AAC1(YMR056C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ATG5(YPL149W)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATO3(YDR384C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AUS1(YOR011W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:AXL2(YIL140W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:CRG1(YHR209W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAN1(YJR150C)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DIC1(YLR348C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DTR1(YBR180W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMP65(YER140W_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERJ5(YFR041C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:ERT1(YBR239C_p)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:GLG2(YJL137C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HRD1(YOL013C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HSM3(YBR272C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST2(YPL015C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IBA57(YJR122W)|FD-Score:-4.81|P-value:7.58E-7||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMP2(YMR035W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRC14(YOR135C_d)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JLP2(YMR132C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KAP123(YER110C)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KIN4(YOR233W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LEA1(YPL213W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LEU3(YLR451W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MDH2(YOL126C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MIS1(YBR084W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MMS1(YPR164W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MSN4(YKL062W)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:NAP1(YKR048C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NBA1(YOL070C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NCS6(YGL211W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NUC1(YJL208C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUM1(YDR150W)|FD-Score:9.8|P-value:5.58E-23||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCH1(YGL038C)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:OGG1(YML060W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSH6(YKR003W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PES4(YFR023W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PHO87(YCR037C)|FD-Score:7.19|P-value:3.19E-13||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF4(YGL014W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:SAE2(YGL175C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SLK19(YOR195W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SNX3(YOR357C)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SSB1(YDL229W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STF1(YDL130W-A)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TMA10(YLR327C)|FD-Score:8.67|P-value:2.12E-18||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TPO4(YOR273C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:VMR1(YHL035C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VTC3(YPL019C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-4.46|P-value:4.01E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR048C(YDR048C_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR338C(YDR338C_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YER130C(YER130C_p)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGR093W(YGR093W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YGR228W(YGR228W_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHL012W(YHL012W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YLL059C(YLL059C_d)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR446W(YLR446W_p)|FD-Score:-4.09|P-value:2.11E-5||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML034C-A(YML034C-A_d)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL303W(YNL303W_d)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR117W(YPR117W_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function Gene:AAC1(YMR056C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress Gene:ATG5(YPL149W)|FD-Score:4.54|P-value:2.87E-6||SGD DESC:Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation Gene:ATO3(YDR384C)|FD-Score:3.55|P-value:1.92E-4||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:AUS1(YOR011W)|FD-Score:3.13|P-value:8.78E-4||SGD DESC:Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen Gene:AXL2(YIL140W)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Integral plasma membrane protein required for axial budding in haploid cells, localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate Gene:CRG1(YHR209W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:DAN1(YJR150C)|FD-Score:-3.81|P-value:6.93E-5||SGD DESC:Cell wall mannoprotein with similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth Gene:DIC1(YLR348C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:DTR1(YBR180W)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:EMP65(YER140W_p)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:ERJ5(YFR041C)|FD-Score:3.46|P-value:2.68E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:ERT1(YBR239C_p)|FD-Score:-3.27|P-value:5.39E-4||SGD DESC:Transcriptional regulator of nonfermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition Gene:GLG2(YJL137C)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication Gene:HRD1(YOL013C)|FD-Score:-4.12|P-value:1.88E-5||SGD DESC:Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon Gene:HSM3(YBR272C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; related to human 19S subunit S5b; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation Gene:HST2(YPL015C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Cytoplasmic member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export Gene:IBA57(YJR122W)|FD-Score:-4.81|P-value:7.58E-7||SGD DESC:Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system Gene:IMP2(YMR035W)|FD-Score:-3.82|P-value:6.60E-5||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IRC14(YOR135C_d)|FD-Score:-5.06|P-value:2.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:JLP2(YMR132C_p)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) Gene:KAP123(YER110C)|FD-Score:3.77|P-value:8.10E-5||SGD DESC:Karyopherin beta, mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1 Gene:KIN4(YOR233W)|FD-Score:-3.39|P-value:3.53E-4||SGD DESC:Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint is activated; localized asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication Gene:KRE6(YPR159W)|FD-Score:4.24|P-value:1.09E-5||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:LEA1(YPL213W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Component of U2 snRNP; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein Gene:LEU3(YLR451W)|FD-Score:3.13|P-value:8.70E-4||SGD DESC:Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation Gene:MDH2(YOL126C)|FD-Score:3.52|P-value:2.19E-4||SGD DESC:Cytoplasmic malate dehydrogenase, one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1 Gene:MIS1(YBR084W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase Gene:MMS1(YPR164W)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MSN4(YKL062W)|FD-Score:-3.88|P-value:5.22E-5||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN4 has a paralog, MSN2, that arose from the whole genome duplication Gene:NAP1(YKR048C)|FD-Score:4.7|P-value:1.32E-6||SGD DESC:Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress Gene:NBA1(YOL070C)|FD-Score:-3.14|P-value:8.43E-4||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:NCS6(YGL211W)|FD-Score:4.21|P-value:1.30E-5||SGD DESC:Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:NUC1(YJL208C)|FD-Score:-3.32|P-value:4.54E-4||SGD DESC:Major mitochondrial nuclease, has RNAse and DNA endo- and exonucleolytic activities; has roles in mitochondrial recombination, apoptosis and maintenance of polyploidy Gene:NUM1(YDR150W)|FD-Score:9.8|P-value:5.58E-23||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OCH1(YGL038C)|FD-Score:4.47|P-value:3.84E-6||SGD DESC:Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins Gene:OGG1(YML060W)|FD-Score:4.09|P-value:2.13E-5||SGD DESC:Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance Gene:OSH6(YKR003W)|FD-Score:-3.18|P-value:7.31E-4||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:PES4(YFR023W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PHO87(YCR037C)|FD-Score:7.19|P-value:3.19E-13||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:PTH2(YBL057C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:One of two (see also PTH1) mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth Gene:PUF4(YGL014W)|FD-Score:3.93|P-value:4.29E-5||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:SAE2(YGL175C)|FD-Score:3.74|P-value:9.26E-5||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SLK19(YOR195W)|FD-Score:-3.32|P-value:4.52E-4||SGD DESC:Kinetochore-associated protein required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate Gene:SNX3(YOR357C)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SSB1(YDL229W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication Gene:STB2(YMR053C)|FD-Score:3.19|P-value:7.15E-4||SGD DESC:Protein that interacts with Sin3p in a two-hybrid assay and is part of a large protein complex with Sin3p and Stb1p Gene:STF1(YDL130W-A)|FD-Score:5.01|P-value:2.79E-7||SGD DESC:Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress Gene:TMA10(YLR327C)|FD-Score:8.67|P-value:2.12E-18||SGD DESC:Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress Gene:TPO4(YOR273C)|FD-Score:3.8|P-value:7.28E-5||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:VMR1(YHL035C)|FD-Score:3.3|P-value:4.82E-4||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VTC3(YPL019C)|FD-Score:3.96|P-value:3.68E-5||SGD DESC:Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-4.46|P-value:4.01E-6||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR034W-B(YDR034W-B_p)|FD-Score:3.38|P-value:3.67E-4||SGD DESC:Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YDR048C(YDR048C_d)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR271C(YDR271C_d)|FD-Score:3.21|P-value:6.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W Gene:YDR338C(YDR338C_p)|FD-Score:4|P-value:3.23E-5||SGD DESC:Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily Gene:YER130C(YER130C_p)|FD-Score:4.48|P-value:3.69E-6||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YGR093W(YGR093W_p)|FD-Score:-3.15|P-value:8.19E-4||SGD DESC:Putative protein of unconfirmed function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:YGR169C-A(YGR169C-A_p)|FD-Score:3.12|P-value:9.18E-4||SGD DESC:Putative protein of unknown function; YGR169C-A has a paralog, YJR005C-A, that arose from the whole genome duplication Gene:YGR228W(YGR228W_d)|FD-Score:3.33|P-value:4.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YHL012W(YHL012W_p)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication Gene:YLL059C(YLL059C_d)|FD-Score:4.65|P-value:1.65E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR040C(YLR040C_p)|FD-Score:-3.12|P-value:9.18E-4||SGD DESC:Protein of unknown function; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W; mRNA identified as translated by ribosome profiling data; YLR040C is not essential Gene:YLR446W(YLR446W_p)|FD-Score:-4.09|P-value:2.11E-5||SGD DESC:Putative hexokinase; transcript is upregulated during sporulation and the unfolded protein response; YLR446W is not an essential gene Gene:YML034C-A(YML034C-A_d)|FD-Score:5.51|P-value:1.80E-8||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR075C-A(YMR075C-A_d)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RCO1/YMR075W Gene:YNL303W(YNL303W_d)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR117W(YPR117W_p)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR107C7.416.54E-142.90CDC12Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
YCR054C4.513.28E-60.16CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YDL150W4.356.84E-60.33RPC53RNA polymerase III subunit C53
YDL014W4.022.91E-50.10NOP1Nucleolar protein, component of the small subunit processome complex, which is required for processing of pre-18S rRNA; has similarity to mammalian fibrillarin
YMR288W3.924.47E-50.12HSH155U2-snRNP associated splicing factor that forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
YGL030W3.807.34E-50.14RPL30Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog
YBR089W_d3.651.30E-40.06YBR089W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
YOL077C3.591.65E-40.22BRX1Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
YHR005C-A3.373.71E-40.07TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YIL021W3.304.79E-40.10RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YOR020C3.216.74E-40.08HSP10Mitochondrial matrix co-chaperonin that inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
YJR065C3.138.73E-40.02ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
YGL040C3.119.48E-40.15HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YOR260W2.950.001580.01GCD1Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YGR158C2.950.001600.05MTR3Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR150W9.805.58E-23NUM1Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
YLR327C8.672.12E-18TMA10Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
YCR037C7.193.19E-13PHO87Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
YML034C-A_d5.511.80E-8YML034C-A_dDubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDL130W-A5.012.79E-7STF1Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress
YKR048C4.701.32E-6NAP1Protein that interacts with mitotic cyclin Clb2p; required for the regulation of microtubule dynamics during mitosis; controls bud morphogenesis; involved in the transport of H2A and H2B histones to the nucleus; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
YLL059C_d4.651.65E-6YLL059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR164W4.631.81E-6MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YPL149W4.542.87E-6ATG5Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; conjugated Atg12p also forms a complex with Atg16p that is essential for autophagosome formation
YER130C_p4.483.69E-6YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YGL038C4.473.84E-6OCH1Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
YPR159W4.241.09E-5KRE6Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p
YGL211W4.211.30E-5NCS6Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
YML060W4.092.13E-5OGG1Mitochondrial glycosylase/lyase that specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
YDR338C_p4.003.23E-5YDR338C_pPutative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily

GO enrichment analysis for SGTC_2247
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1132.95E-18SGTC_2251lansoprazole 556.0 μMNIH Clinical Collection38830.0379747
0.0756.47E-9SGTC_3813461-2219 42.7 μMChemDiv (Drug-like library)28831590.015625copper-dependent oxidative stress
0.0731.62E-8SGTC_1513013-0144 2.3 μMChemDiv (Drug-like library)9065580.0422535iron homeostasis
0.0713.92E-8SGTC_14063909-7906 11.9 μMChemDiv (Drug-like library)X14060.02816960S ribosome export
0.0656.91E-7SGTC_24705664817 21.7 μMMiscellaneous28595610.056338
0.0622.02E-6SGTC_28307992007 71.4 μMChembridge (Drug-like library)19520750.0131579
0.0587.09E-6SGTC_920063-0024 660.9 μMChemDiv (Drug-like library)35944370.0357143
0.0587.80E-6SGTC_23296138490 200.0 μMChembridge (Fragment library)7200190.0655738
0.0561.50E-5SGTC_1543azetidyl-2-carboxylic acid 198.0 μMTimTec (Pure natural product library)164860.0888889
0.0552.11E-5SGTC_2170morpholine 200.0 μMChembridge (Fragment library)1211710.0819672
0.0526.73E-5SGTC_21846075246 200.0 μMChembridge (Fragment library)22673200.0327869
0.0526.79E-5SGTC_290299-0004 13.0 μMChemDiv (Drug-like library)168710.037037
0.0518.03E-5SGTC_30669117245 49.5 μMChembridge (Drug-like library)171769800.056338
0.0491.66E-4SGTC_22127123476 200.0 μMChembridge (Fragment library)10857150.0169492
0.0482.02E-4SGTC_8540422-0053 192.0 μMChemDiv (Drug-like library)35967280.0149254PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2417cerulenin830 nM15282054ICCB bioactive library223.268281.0913
SGTC_409arachidonic acid100 μM0.288889231Miscellaneous304.466886.43912
SGTC_410α-linolenic acid100 μM0.282609860Miscellaneous278.42965.97112excess fatty acid
SGTC_475α-linolenic acid10 μM0.2826095280934ICCB bioactive library278.42965.97112excess fatty acid
SGTC_476mead acid10 μM0.2826095312531ICCB bioactive library306.482766.88312
SGTC_478N-arachidonylglycine10 μM0.255283389ICCB bioactive library361.51825.49923
SGTC_4732-arachidonoylglycerol10 μM0.2452835282280ICCB bioactive library378.545425.61424
SGTC_477mead ethanolamide10 μM0.24528316061185ICCB bioactive library349.550565.92222
SGTC_479n-linoleoylglycine10 μM0.2452836433346ICCB bioactive library337.49685.47623
SGTC_1876myriocin605 nM0.171875301119Miscellaneous401.537460.28457
SGTC_1573oleylamine1.06 μM0.1590915356789TimTec (Pure natural product library)267.493086.62311