nsc-122657

1-benzylsulfonyl-2,4-dinitrobenzene

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_225
Screen concentration 1.0 μM
Source Miscellaneous
PubChem CID 275685
SMILES C1=CC=C(C=C1)CS(=O)(=O)C2=C(C=C(C=C2)[N+](=O)[O-])[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(c(c1)[N+](=O)[O-])S(=O)(=O)Cc2ccccc2
Molecular weight 322.2933
ALogP 2.73
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.58
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 275685
Download HIP data (tab-delimited text)  (excel)
Gene:DPB2(YPR175W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:FOL1(YNL256W)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:FOL2(YGR267C)|FD-Score:-3.12|P-value:8.92E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:LCB2(YDR062W)|FD-Score:3.18|P-value:7.28E-4|Clearance:0.18||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MPS3(YJL019W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MYO1(YHR023W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.14||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:QNS1(YHR074W)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.17||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPN7(YPR108W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.3||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:TSC13(YDL015C)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.18||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:TUB4(YLR212C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.02||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YJL015C(YJL015C_d)|FD-Score:4.31|P-value:8.06E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W Gene:DPB2(YPR175W)|FD-Score:-3.51|P-value:2.22E-4|Clearance:0||SGD DESC:Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate Gene:FOL1(YNL256W)|FD-Score:-3.4|P-value:3.40E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:FOL2(YGR267C)|FD-Score:-3.12|P-value:8.92E-4|Clearance:0||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:LCB2(YDR062W)|FD-Score:3.18|P-value:7.28E-4|Clearance:0.18||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MPS3(YJL019W)|FD-Score:-3.43|P-value:3.02E-4|Clearance:0||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:MYO1(YHR023W)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.14||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:QNS1(YHR074W)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.17||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPN7(YPR108W)|FD-Score:3.48|P-value:2.49E-4|Clearance:0.3||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:TSC13(YDL015C)|FD-Score:3.82|P-value:6.76E-5|Clearance:0.18||SGD DESC:Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress Gene:TUB4(YLR212C)|FD-Score:3.64|P-value:1.38E-4|Clearance:0.02||SGD DESC:Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress Gene:YJL015C(YJL015C_d)|FD-Score:4.31|P-value:8.06E-6|Clearance:0.33||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 275685
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AVT1(YJR001W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CLB3(YDL155W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CTR1(YPR124W)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:5.71|P-value:5.74E-9||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DGR1(YNL130C-A_p)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPH1(YIL103W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DSF2(YBR007C_p)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FBP26(YJL155C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FRA2(YGL220W)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GUP1(YGL084C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IDH2(YOR136W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC21(YMR073C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LSB1(YGR136W)|FD-Score:-4.03|P-value:2.73E-5||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:MSA1(YOR066W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB2(YGR014W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:PCA1(YBR295W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PPT2(YPL148C)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:REC104(YHR157W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:SEA4(YBL104C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SOL3(YHR163W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:YDR008C(YDR008C_d)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YLR271W(YLR271W_p)|FD-Score:13.4|P-value:2.06E-41||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR419W(YLR419W_p)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACE2(YLR131C)|FD-Score:3.36|P-value:3.90E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AVT1(YJR001W)|FD-Score:4.05|P-value:2.52E-5||SGD DESC:Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CLB3(YDL155W)|FD-Score:4.04|P-value:2.62E-5||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:CTR1(YPR124W)|FD-Score:5.74|P-value:4.79E-9||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTS2(YDR371W_p)|FD-Score:5.71|P-value:5.74E-9||SGD DESC:Putative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect Gene:DGR1(YNL130C-A_p)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPH1(YIL103W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p Gene:DSF2(YBR007C_p)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:-3.92|P-value:4.34E-5||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FBP26(YJL155C)|FD-Score:3.09|P-value:9.86E-4||SGD DESC:Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress Gene:FRA2(YGL220W)|FD-Score:5.92|P-value:1.62E-9||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GUP1(YGL084C)|FD-Score:4.85|P-value:6.12E-7||SGD DESC:Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport Gene:IDH2(YOR136W)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated Gene:IRC21(YMR073C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:LSB1(YGR136W)|FD-Score:-4.03|P-value:2.73E-5||SGD DESC:Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress Gene:MSA1(YOR066W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Activator of G1-specific transcription factors, MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation Gene:MSB2(YGR014W)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Mucin family member involved in signaling; functions in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; is processed into secreted and cell-associated forms by the aspartyl protease, Yps1p; also functions as an osmosensor in parallel to the Sho1p-mediated pathway; potential Cdc28p substrate Gene:PCA1(YBR295W)|FD-Score:-3.1|P-value:9.83E-4||SGD DESC:Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function Gene:PPT2(YPL148C)|FD-Score:4.92|P-value:4.34E-7||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:REC104(YHR157W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination Gene:SEA4(YBL104C)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SOL3(YHR163W)|FD-Score:3.84|P-value:6.05E-5||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:TRP3(YKL211C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TSC3(YBR058C-A)|FD-Score:6.99|P-value:1.35E-12||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBP1(YDL122W)|FD-Score:3.24|P-value:6.03E-4||SGD DESC:Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains Gene:YDR008C(YDR008C_d)|FD-Score:3.9|P-value:4.78E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR111W(YGR111W_p)|FD-Score:3.12|P-value:8.96E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YLR271W(YLR271W_p)|FD-Score:13.4|P-value:2.06E-41||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YLR419W(YLR419W_p)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:-3.2|P-value:6.81E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPL113C(YPL113C_p)|FD-Score:-3.09|P-value:9.84E-4||SGD DESC:Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL015C_d4.318.06E-60.33YJL015C_dDubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
YHR074W3.993.35E-50.17QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YDL015C3.826.76E-50.18TSC13Enoyl reductase; catalyzes the last step in each cycle of very long chain fatty acid elongation; localizes to the ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Fen1p and Sur4p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress
YLR212C3.641.38E-40.02TUB4Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
YHR023W3.621.48E-40.14MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YPR108W3.482.49E-40.30RPN7Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
YDR062W3.187.28E-40.18LCB2Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
YDR240C3.000.001330.08SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YDR224C2.930.001710.07HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YGR147C2.860.002140.05NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YBR234C2.800.002520.00ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YLR355C2.800.002550.00ILV5Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
YML114C2.800.002580.07TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YOR250C2.720.003230.04CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YGR190C_d2.690.003610.00YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR271W_p13.402.06E-41YLR271W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
YBR058C-A6.991.35E-12TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YGL220W5.921.62E-9FRA2Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel
YPR124W5.744.79E-9CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YDR371W_p5.715.74E-9CTS2_pPutative chitinase, functionally complements A. gossypii cts2 mutant sporulation defect
YPL148C4.924.34E-7PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YGL084C4.856.12E-7GUP1Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; proposed to be involved in glycerol transport
YLR419W_p4.356.94E-6YLR419W_pPutative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
YJR001W4.052.52E-5AVT1Vacuolar transporter, imports large neutral amino acids into the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
YDL155W4.042.62E-5CLB3B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress
YDR008C_d3.904.78E-5YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YHR163W3.846.05E-5SOL36-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
YKL211C3.522.20E-4TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YIL103W3.482.46E-4DPH1Protein required, along with Dph2p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph2p and Kti11p
YOR136W3.393.46E-4IDH2Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle; phosphorylated

GO enrichment analysis for SGTC_225
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3681.44E-188SGTC_223busulfan 5.0 mMMiscellaneous24780.0888889
0.2665.96E-96SGTC_220paclitaxel 373.4 μMMiscellaneous46660.0614035
0.2642.29E-94SGTC_224benfluorex 43.9 μMMiscellaneous23180.15942
0.2359.03E-75SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0806452TSC3-RPN4
0.2308.67E-72SGTC_7734358-1457 44.6 μMChemDiv (Drug-like library)11868860.0821918
0.2061.56E-57SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0983607TSC3-RPN4
0.2056.07E-57SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.031746TSC3-RPN4
0.2049.40E-57SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0933333TSC3-RPN4
0.2011.41E-54SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.0606061
0.1866.75E-47SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.22807TSC3-RPN4
0.1852.10E-46SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0869565TSC3-RPN4
0.1799.17E-44SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0483871TSC3-RPN4
0.1778.30E-43SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.237288TSC3-RPN4
0.1763.76E-42SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0810811
0.1742.09E-41SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.125TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_230213-001140 μM0.409091627608ChemDiv (Drug-like library)304.215123.06317copper-dependent oxidative stress
SGTC_260302-0163425.16 μM0.3958333100915ChemDiv (Drug-like library)275.216962.92626heme biosynthesis & mitochondrial translocase
SGTC_850868-0259160.06 μM0.37583173ChemDiv (Drug-like library)256.256682.5431360S ribosome export
SGTC_3710195-00067.36 μM0.3720933928593ChemDiv (Drug-like library)253.209682.70204
SGTC_7930488-0545106 μM0.369565292548ChemDiv (Drug-like library)237.212042.24105
SGTC_507nimesulide162 μM0.3584914495Miscellaneous308.309782.19516
SGTC_458nppb133 μM0.3571434549ICCB bioactive library300.309243.52325
SGTC_410478-063282.64 μM0.3541673750424ChemDiv (Drug-like library)229.234642.52724
SGTC_6260214-000933 μM0.3529413437874ChemDiv (Drug-like library)332.225222.29717copper-dependent oxidative stress
SGTC_30601327-002712.5 μM0.35087716000455ChemDiv (Drug-like library)454.123145.74815