7938975

N-(3-chloro-2-methylphenyl)-5-methyl-4,5-dihydro-1,3-thiazol-2-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2254
Screen concentration 95.2 μM
Source Chembridge (Fragment library)
PubChem CID 2969277
SMILES CC1CN=C(S1)NC2=C(C(=CC=C2)Cl)C
Standardized SMILES CC1CN=C(Nc2cccc(Cl)c2C)S1
Molecular weight 240.7523
ALogP 3.76
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.93
% growth inhibition (Hom. pool) 19.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2969277
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.17||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP3(YJR065C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.15||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BUR6(YER159C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.05||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CBF2(YGR140W)|FD-Score:3.1|P-value:9.60E-4|Clearance:0.12||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CCT3(YJL014W)|FD-Score:5.66|P-value:7.78E-9|Clearance:0.62||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:3.26|P-value:5.62E-4|Clearance:0.09||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:GLC7(YER133W)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.28||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HYM1(YKL189W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.01||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:IRR1(YIL026C)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.06||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCD1(YDL003W)|FD-Score:3.61|P-value:1.50E-4|Clearance:0.03||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:PRE2(YPR103W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.03||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPB3(YIL021W)|FD-Score:-4.02|P-value:2.94E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RSC9(YML127W)|FD-Score:-4.98|P-value:3.21E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SGV1(YPR161C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.01||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPT6(YGR116W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.08||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSU72(YNL222W)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.07||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:STU1(YBL034C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.45||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SUI1(YNL244C)|FD-Score:4.28|P-value:9.25E-6|Clearance:0.16||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TAF6(YGL112C)|FD-Score:4.81|P-value:7.44E-7|Clearance:0.08||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC1(YBR123C)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP8(YGR128C)|FD-Score:5.04|P-value:2.37E-7|Clearance:0.16||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YOR169C(YOR169C_d)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W Gene:ARC40(YBR234C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.17||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARP3(YJR065C)|FD-Score:3.42|P-value:3.18E-4|Clearance:0.15||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:BUR6(YER159C)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.05||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CBF2(YGR140W)|FD-Score:3.1|P-value:9.60E-4|Clearance:0.12||SGD DESC:Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress Gene:CCT3(YJL014W)|FD-Score:5.66|P-value:7.78E-9|Clearance:0.62||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC25(YLR310C)|FD-Score:3.26|P-value:5.62E-4|Clearance:0.09||SGD DESC:Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1 Gene:GLC7(YER133W)|FD-Score:3.9|P-value:4.89E-5|Clearance:0.28||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HYM1(YKL189W)|FD-Score:3.51|P-value:2.27E-4|Clearance:0.01||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response Gene:IRR1(YIL026C)|FD-Score:4.87|P-value:5.46E-7|Clearance:0.06||SGD DESC:Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability Gene:MCD1(YDL003W)|FD-Score:3.61|P-value:1.50E-4|Clearance:0.03||SGD DESC:Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase Gene:PRE2(YPR103W)|FD-Score:3.59|P-value:1.68E-4|Clearance:0.03||SGD DESC:Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome Gene:RPB3(YIL021W)|FD-Score:-4.02|P-value:2.94E-5|Clearance:0||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RSC9(YML127W)|FD-Score:-4.98|P-value:3.21E-7|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SGV1(YPR161C)|FD-Score:3.26|P-value:5.49E-4|Clearance:0.01||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SPT6(YGR116W)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.08||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:SSU72(YNL222W)|FD-Score:3.17|P-value:7.60E-4|Clearance:0.07||SGD DESC:Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo Gene:STU1(YBL034C)|FD-Score:4.73|P-value:1.10E-6|Clearance:0.45||SGD DESC:Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles Gene:SUI1(YNL244C)|FD-Score:4.28|P-value:9.25E-6|Clearance:0.16||SGD DESC:Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase Gene:TAF6(YGL112C)|FD-Score:4.81|P-value:7.44E-7|Clearance:0.08||SGD DESC:Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4 Gene:TFC1(YBR123C)|FD-Score:-3.3|P-value:4.91E-4|Clearance:0||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:UTP8(YGR128C)|FD-Score:5.04|P-value:2.37E-7|Clearance:0.16||SGD DESC:Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YOR169C(YOR169C_d)|FD-Score:3.95|P-value:3.85E-5|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2969277
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM4(YBR194W)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALG5(YPL227C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARC18(YLR370C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG3(YJL088W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:29|P-value:1.24E-184||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:6.34|P-value:1.12E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP6(YLR085C)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASG1(YIL130W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATP3(YBR039W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK1(YJL095W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM2(YER155C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CLN3(YAL040C)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG7(YGL005C)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX18(YGR062C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DAL81(YIR023W)|FD-Score:12.3|P-value:3.48E-35||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIA4(YHR011W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DLD2(YDL178W)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EFM2(YBR271W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ELC1(YPL046C)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG2(YMR202W)|FD-Score:14|P-value:3.92E-45||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAS1(YMR307W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN2(YDR283C)|FD-Score:6.36|P-value:1.04E-10||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:7.46|P-value:4.40E-14||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GLY1(YEL046C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:GYP1(YOR070C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HCR1(YLR192C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEF3(YNL014W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRS4(YKR019C)|FD-Score:11.6|P-value:1.91E-31||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:5.8|P-value:3.26E-9||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LDB7(YBL006C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEU9(YOR108W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAG1(YER142C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MDM20(YOL076W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEF1(YLR069C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL4(YLR439W)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS12(YNR036C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS5(YBR251W)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSW1(YDR268W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NBP2(YDR162C)|FD-Score:6.42|P-value:6.93E-11||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NVJ1(YHR195W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OCA6(YDR067C_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI11(YPR044C_d)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PEP3(YLR148W)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET111(YMR257C)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET54(YGR222W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHO4(YFR034C)|FD-Score:7.09|P-value:6.85E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PMP1(YCR024C-A)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POM33(YLL023C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAV1(YJR033C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:6.43|P-value:6.56E-11||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA12(YJR063W)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL19B(YBL027W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-8.77|P-value:8.82E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM18(YER050C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SAC6(YDR129C)|FD-Score:15.2|P-value:3.07E-52||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMI1(YGR229C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF7(YLR025W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPC2(YML055W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT21(YMR179W)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUR7(YML052W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SUV3(YPL029W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWC3(YAL011W)|FD-Score:8.53|P-value:7.63E-18||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWR1(YDR334W)|FD-Score:4.75|P-value:9.99E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THO2(YNL139C)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIM8(YJR135W-A)|FD-Score:-5.52|P-value:1.67E-8||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TLG2(YOL018C)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:18.9|P-value:8.12E-80||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:21.6|P-value:1.56E-103||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:35.2|P-value:9.89E-272||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.93|P-value:1.51E-23||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:6.89|P-value:2.75E-12||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:11.8|P-value:3.25E-32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VPS25(YJR102C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR056W(YBR056W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR102C(YCR102C_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR008C(YDR008C_d)|FD-Score:12.6|P-value:1.35E-36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR228W(YGR228W_d)|FD-Score:5.28|P-value:6.61E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML007C-A(YML007C-A_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR262W(YMR262W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR066C(YNR066C_p)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPK1(YKL126W)|FD-Score:-4.78|P-value:8.66E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPS7(YDR349C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YUR1(YJL139C)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ACO1(YLR304C)|FD-Score:4.56|P-value:2.55E-6||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:AIM4(YBR194W)|FD-Score:-3.84|P-value:6.07E-5||SGD DESC:Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress Gene:ALG5(YPL227C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:ARC18(YLR370C)|FD-Score:3.19|P-value:7.18E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARG3(YJL088W)|FD-Score:5.54|P-value:1.51E-8||SGD DESC:Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine Gene:ARO1(YDR127W)|FD-Score:29|P-value:1.24E-184||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO2(YGL148W)|FD-Score:4.11|P-value:1.97E-5||SGD DESC:Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress Gene:ARO3(YDR035W)|FD-Score:6.34|P-value:1.12E-10||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ARO7(YPR060C)|FD-Score:7.29|P-value:1.50E-13||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP6(YLR085C)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:ASG1(YIL130W)|FD-Score:3.19|P-value:7.13E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATP3(YBR039W)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:BCK1(YJL095W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BEM2(YER155C)|FD-Score:-3.59|P-value:1.66E-4||SGD DESC:Rho GTPase activating protein (RhoGAP) involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence Gene:CLN3(YAL040C)|FD-Score:3.9|P-value:4.74E-5||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG7(YGL005C)|FD-Score:4.48|P-value:3.73E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG8(YML071C)|FD-Score:5.19|P-value:1.06E-7||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COX18(YGR062C)|FD-Score:-3.41|P-value:3.24E-4||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:DAL81(YIR023W)|FD-Score:12.3|P-value:3.48E-35||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DFG16(YOR030W)|FD-Score:5.48|P-value:2.08E-8||SGD DESC:Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p Gene:DIA4(YHR011W)|FD-Score:4.05|P-value:2.59E-5||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DLD2(YDL178W)|FD-Score:-3.39|P-value:3.56E-4||SGD DESC:D-lactate dehydrogenase, located in the mitochondrial matrix Gene:EFM2(YBR271W)|FD-Score:3.3|P-value:4.75E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:ELC1(YPL046C)|FD-Score:4.43|P-value:4.63E-6||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG2(YMR202W)|FD-Score:14|P-value:3.92E-45||SGD DESC:C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis Gene:GAS1(YMR307W)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCN2(YDR283C)|FD-Score:6.36|P-value:1.04E-10||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN4(YEL009C)|FD-Score:7.46|P-value:4.40E-14||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GCV3(YAL044C)|FD-Score:-3.62|P-value:1.49E-4||SGD DESC:H subunit of the mitochondrial glycine decarboxylase complex, required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF Gene:GLY1(YEL046C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:GUF1(YLR289W)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:GYP1(YOR070C)|FD-Score:3.48|P-value:2.53E-4||SGD DESC:Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion Gene:HCR1(YLR192C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of translation initiation factor 3 (eIF3) and required for processing of 20S pre-rRNA; binds to eIF3 subunits Rpg1p and Prt1p and 18S rRNA Gene:HEF3(YNL014W)|FD-Score:-3.19|P-value:7.21E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:6.22|P-value:2.55E-10||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:IRS4(YKR019C)|FD-Score:11.6|P-value:1.91E-31||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:ISM1(YPL040C)|FD-Score:5.8|P-value:3.26E-9||SGD DESC:Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth Gene:LDB7(YBL006C)|FD-Score:-3.17|P-value:7.62E-4||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LEU9(YOR108W)|FD-Score:3.94|P-value:4.05E-5||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:MAG1(YER142C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:3-methyl-adenine DNA glycosylase; involved in protecting DNA against alkylating agents; initiates base excision repair by removing damaged bases to create abasic sites that are subsequently repaired; protein abundance increases in response to DNA replication stress Gene:MDM20(YOL076W)|FD-Score:4.1|P-value:2.09E-5||SGD DESC:Non-catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly Gene:MEF1(YLR069C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Mitochondrial elongation factor involved in translational elongation Gene:MRPL4(YLR439W)|FD-Score:3.75|P-value:8.89E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit Gene:MRPS12(YNR036C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins Gene:MRPS5(YBR251W)|FD-Score:5.02|P-value:2.64E-7||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSE1(YOL033W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Mitochondrial glutamyl-tRNA synthetase, predicted to be palmitoylated Gene:MSW1(YDR268W)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NBP2(YDR162C)|FD-Score:6.42|P-value:6.93E-11||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NVJ1(YHR195W)|FD-Score:-3.25|P-value:5.75E-4||SGD DESC:Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN) Gene:OCA6(YDR067C_p)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT Gene:OPI11(YPR044C_d)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO Gene:PEP3(YLR148W)|FD-Score:4.33|P-value:7.48E-6||SGD DESC:Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis Gene:PET111(YMR257C)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane Gene:PET54(YGR222W)|FD-Score:3.39|P-value:3.52E-4||SGD DESC:Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing Gene:PHO4(YFR034C)|FD-Score:7.09|P-value:6.85E-13||SGD DESC:Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability Gene:PMP1(YCR024C-A)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:POM33(YLL023C)|FD-Score:3.19|P-value:7.22E-4||SGD DESC:Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress Gene:PRO2(YOR323C)|FD-Score:4.03|P-value:2.78E-5||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:RAV1(YJR033C)|FD-Score:3.25|P-value:5.72E-4||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:REG1(YDR028C)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:6.43|P-value:6.56E-11||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:ROM2(YLR371W)|FD-Score:-4.73|P-value:1.12E-6||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPA12(YJR063W)|FD-Score:4.81|P-value:7.50E-7||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPL19B(YBL027W)|FD-Score:3.22|P-value:6.33E-4||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RPL23B(YER117W)|FD-Score:-3.21|P-value:6.71E-4||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:-8.77|P-value:8.82E-19||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RSM18(YER050C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein Gene:SAC6(YDR129C)|FD-Score:15.2|P-value:3.07E-52||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SLT2(YHR030C)|FD-Score:5.81|P-value:3.09E-9||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SMI1(YGR229C)|FD-Score:5.12|P-value:1.53E-7||SGD DESC:Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity Gene:SNF7(YLR025W)|FD-Score:4.18|P-value:1.47E-5||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SPC2(YML055W)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Subunit of signal peptidase complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC25 Gene:SPT21(YMR179W)|FD-Score:-4.08|P-value:2.22E-5||SGD DESC:Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; functionally related to Spt10p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SUR7(YML052W)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SUV3(YPL029W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:ATP-dependent RNA helicase, component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron Gene:SWC3(YAL011W)|FD-Score:8.53|P-value:7.63E-18||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWR1(YDR334W)|FD-Score:4.75|P-value:9.99E-7||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYS1(YJL004C)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THO2(YNL139C)|FD-Score:4.57|P-value:2.43E-6||SGD DESC:Subunit of the THO complex, which is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids Gene:TIM8(YJR135W-A)|FD-Score:-5.52|P-value:1.67E-8||SGD DESC:Mitochondrial intermembrane space protein, forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome Gene:TLG2(YOL018C)|FD-Score:4.44|P-value:4.40E-6||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TRP1(YDR007W)|FD-Score:18.9|P-value:8.12E-80||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:21.6|P-value:1.56E-103||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:35.2|P-value:9.89E-272||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:9.93|P-value:1.51E-23||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TRP5(YGL026C)|FD-Score:6.89|P-value:2.75E-12||SGD DESC:Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:11.8|P-value:3.25E-32||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAM3(YOR106W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VPS25(YJR102C)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS45(YGL095C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:5.02|P-value:2.59E-7||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:5.18|P-value:1.13E-7||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YAF9(YNL107W)|FD-Score:3.22|P-value:6.32E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR056W(YBR056W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR196C-A(YBR196C-A_p)|FD-Score:4|P-value:3.14E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YCR102C(YCR102C_p)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR008C(YDR008C_d)|FD-Score:12.6|P-value:1.35E-36||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR445C(YDR445C_d)|FD-Score:-3.12|P-value:9.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YGK3(YOL128C)|FD-Score:-4.26|P-value:1.01E-5||SGD DESC:Protein kinase related to mammalian glycogen synthase kinases of the GSK-3 family; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation Gene:YGR228W(YGR228W_d)|FD-Score:5.28|P-value:6.61E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YLL006W-A(YLL006W-A_p)|FD-Score:-3.26|P-value:5.60E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YML007C-A(YML007C-A_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR262W(YMR262W_p)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene Gene:YNL195C(YNL195C_p)|FD-Score:-5.2|P-value:1.01E-7||SGD DESC:Putative protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YNR066C(YNR066C_p)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:Putative membrane-localized protein of unknown function Gene:YPK1(YKL126W)|FD-Score:-4.78|P-value:8.66E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPS7(YDR349C)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative GPI-anchored aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum Gene:YUR1(YJL139C)|FD-Score:3.75|P-value:8.81E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YJL014W5.667.78E-90.62CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGR128C5.042.37E-70.16UTP8Nucleolar protein required for export of tRNAs from the nucleus; also copurifies with the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YIL026C4.875.46E-70.06IRR1Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
YGL112C4.817.44E-70.08TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YBL034C4.731.10E-60.45STU1Component of the mitotic spindle that binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles
YNL244C4.289.25E-60.16SUI1Translation initiation factor eIF1; component of a complex involved in recognition of the initiator codon; modulates translation accuracy at the initiation phase
YBR234C4.121.88E-50.17ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YOR169C_d3.953.85E-50.06YOR169C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GLN4/YOR168W
YER133W3.904.89E-50.28GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YDL003W3.611.50E-40.03MCD1Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
YPR103W3.591.68E-40.03PRE2Beta 5 subunit of the 20S proteasome, responsible for the chymotryptic activity of the proteasome
YER159C3.551.90E-40.05BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YKL189W3.512.27E-40.01HYM1Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response
YGR116W3.502.35E-40.08SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YJR065C3.423.18E-40.15ARP3Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YKL211C35.209.89E-272TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR127W29.001.24E-184ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YER090W21.601.56E-103TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR007W18.908.12E-80TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR129C15.203.07E-52SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YMR202W14.003.92E-45ERG2C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
YDR008C_d12.601.35E-36YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YIR023W12.303.48E-35DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YML124C11.803.25E-32TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YKR019C11.601.91E-31IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YDR354W9.931.51E-23TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YAL011W8.537.63E-18SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YEL009C7.464.40E-14GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YPR060C7.291.50E-13ARO7Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
YFR034C7.096.85E-13PHO4Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p,; function is regulated by phosphorylation at multiple sites and by phosphate availability

GO enrichment analysis for SGTC_2254
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.5650SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.0923077
0.5540SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0697674
0.5290SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0735294
0.5120SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0862069TRP & mitochondrial translation
0.5120SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0178571
0.5110SGTC_18825615643 20.0 μMMiscellaneous22530750.133333TRP & mitochondrial translation
0.5090SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.047619
0.5080SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.117647
0.5030SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0757576
0.4960SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0735294
0.4950SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.057971
0.4890SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0759494
0.4850SGTC_2232chlorophetanol 41.5 μMMiscellaneous159070.0980392TRP & mitochondrial translation
0.4830SGTC_24725377652 49.4 μMMiscellaneous16109030.09375TRP & mitochondrial translation
0.4700SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2352902799587.8 μM0.5681822997347Chembridge (Fragment library)226.725743.27212tubulin folding & SWR complex
SGTC_6074491-098666.8 μM0.384615698981ChemDiv (Drug-like library)296.793985.48512heme requiring
SGTC_23649055074200 μM0.3272736470630Chembridge (Fragment library)275.733462.52922
SGTC_15064226-2149212 μM0.3125991114ChemDiv (Drug-like library)355.844564.54115
SGTC_22697935389174.42 μM0.29411897605Chembridge (Fragment library)228.312783.13912tubulin folding & SWR complex
SGTC_2814797748242.97 μM0.2741946456762Chembridge (Drug-like library)343.8009034.00615
SGTC_9933909-875762.7 μM0.2631584288324ChemDiv (Drug-like library)275.75664.01114
SGTC_22446635614200 μM0.245283836499Chembridge (Fragment library)234.079262.01512
SGTC_23819035592200 μM0.2452834749657Chembridge (Fragment library)243.70991.68313
SGTC_1026k007-110530.6 μM0.2413797066794ChemDiv (Drug-like library)280.152543.60713ERAD & cell cycle