7948595

N-(3-carbamoylthiophen-2-yl)furan-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2263
Screen concentration 193.3 μM
Source Chembridge (Fragment library)
PubChem CID 2971371
SMILES C1=COC(=C1)C(=O)NC2=C(C=CS2)C(=O)N
Standardized SMILES NC(=O)c1ccsc1NC(=O)c2occc2
Molecular weight 236.2471
ALogP 0.8
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.77
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2971371
Download HIP data (tab-delimited text)  (excel)
Gene:FIP1(YJR093C)|FD-Score:-4.99|P-value:3.09E-7|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GLC7(YER133W)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPM1(YKL152C)|FD-Score:-4.07|P-value:2.32E-5|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:MAK11(YKL021C)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MEX67(YPL169C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.19||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOP14(YDL148C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.07||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRE8(YML092C)|FD-Score:5.3|P-value:5.70E-8|Clearance:0.61||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAT1(YOR048C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPC53(YDL150W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.14||SGD DESC:RNA polymerase III subunit C53 Gene:RPL18A(YOL120C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.32||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN3(YER021W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN7(YPR108W)|FD-Score:-3.37|P-value:3.70E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPS13(YDR064W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC10(YLR166C)|FD-Score:4.69|P-value:1.38E-6|Clearance:0.43||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SNP1(YIL061C)|FD-Score:3.94|P-value:4.08E-5|Clearance:0.35||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:THS1(YIL078W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.08||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:YLL037W(YLL037W_d)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor Gene:FIP1(YJR093C)|FD-Score:-4.99|P-value:3.09E-7|Clearance:0||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:GLC7(YER133W)|FD-Score:-4.22|P-value:1.25E-5|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:GPM1(YKL152C)|FD-Score:-4.07|P-value:2.32E-5|Clearance:0||SGD DESC:Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis and the reverse reaction during gluconeogenesis Gene:MAK11(YKL021C)|FD-Score:-3.37|P-value:3.72E-4|Clearance:0||SGD DESC:Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats Gene:MEX67(YPL169C)|FD-Score:3.49|P-value:2.43E-4|Clearance:0.19||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NOP14(YDL148C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.07||SGD DESC:Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:PRE8(YML092C)|FD-Score:5.3|P-value:5.70E-8|Clearance:0.61||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAT1(YOR048C)|FD-Score:4.26|P-value:1.03E-5|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RPC53(YDL150W)|FD-Score:3.16|P-value:7.94E-4|Clearance:0.14||SGD DESC:RNA polymerase III subunit C53 Gene:RPL18A(YOL120C)|FD-Score:4.26|P-value:1.04E-5|Clearance:0.32||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN3(YER021W)|FD-Score:3.3|P-value:4.86E-4|Clearance:0.07||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN7(YPR108W)|FD-Score:-3.37|P-value:3.70E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome, similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits Gene:RPS13(YDR064W)|FD-Score:3.51|P-value:2.24E-4|Clearance:0.02||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:SEC10(YLR166C)|FD-Score:4.69|P-value:1.38E-6|Clearance:0.43||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SNP1(YIL061C)|FD-Score:3.94|P-value:4.08E-5|Clearance:0.35||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:THS1(YIL078W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.08||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:YLL037W(YLL037W_d)|FD-Score:3.49|P-value:2.39E-4|Clearance:0.01||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2971371
Download HOP data (tab-delimited text)  (excel)
Gene:ADE1(YAR015W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AIM24(YJR080C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AIM46(YHR199C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ANR2(YKL047W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CLB3(YDL155W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COS111(YBR203W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CPR4(YCR069W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DUS1(YML080W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:FEN1(YCR034W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:ICP55(YER078C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ICT1(YLR099C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC6(YFR043C)|FD-Score:-4.74|P-value:1.06E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAP3(YNL239W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MBB1(YJL199C_d)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDM38(YOL027C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRS4(YKR052C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MTG2(YHR168W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NOT5(YPR072W)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OXR1(YPL196W)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PRM4(YPL156C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRM5(YIL117C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PUT4(YOR348C)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:REG1(YDR028C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RIP1(YEL024W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:SLM5(YCR024C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SSK1(YLR006C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:UBC8(YEL012W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VHC1(YBR235W_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YBL028C(YBL028C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL053W(YBL053W_d)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL086C(YBL086C_p)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL242C(YGL242C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJL107C(YJL107C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJL163C(YJL163C_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function Gene:YOL046C(YOL046C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPR098C(YPR098C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:ADE1(YAR015W)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase, required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress Gene:AIM24(YJR080C)|FD-Score:3.84|P-value:6.08E-5||SGD DESC:Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss Gene:AIM46(YHR199C)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ANR2(YKL047W_p)|FD-Score:3.36|P-value:3.93E-4||SGD DESC:Putative protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:CLB3(YDL155W)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COS111(YBR203W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:CPR4(YCR069W)|FD-Score:3.67|P-value:1.21E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:DUS1(YML080W)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17 Gene:FEN1(YCR034W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:ICP55(YER078C)|FD-Score:3.9|P-value:4.90E-5||SGD DESC:Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family Gene:ICT1(YLR099C)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication Gene:IRC6(YFR043C)|FD-Score:-4.74|P-value:1.06E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:LAP3(YNL239W)|FD-Score:3.89|P-value:4.92E-5||SGD DESC:Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH Gene:MBB1(YJL199C_d)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MDM38(YOL027C)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Mitochondrial protein, forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; roles in protein export and K+/H+ exchange; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome Gene:MRS4(YKR052C)|FD-Score:4.05|P-value:2.61E-5||SGD DESC:Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication Gene:MTG2(YHR168W)|FD-Score:3.55|P-value:1.94E-4||SGD DESC:Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly Gene:NOT5(YPR072W)|FD-Score:3.77|P-value:8.03E-5||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:OXR1(YPL196W)|FD-Score:3.8|P-value:7.13E-5||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PRM4(YPL156C)|FD-Score:-3.61|P-value:1.55E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:PRM5(YIL117C)|FD-Score:3.41|P-value:3.19E-4||SGD DESC:Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling Gene:PUT4(YOR348C)|FD-Score:3.26|P-value:5.47E-4||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:REG1(YDR028C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RIM9(YMR063W)|FD-Score:-3.48|P-value:2.51E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RIP1(YEL024W)|FD-Score:3.88|P-value:5.22E-5||SGD DESC:Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly Gene:SLM5(YCR024C)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Mitochondrial asparaginyl-tRNA synthetase Gene:SSK1(YLR006C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:UBC8(YEL012W)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro Gene:VHC1(YBR235W_p)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YBL028C(YBL028C)|FD-Score:-3.43|P-value:3.04E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:YBL053W(YBL053W_d)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBL086C(YBL086C_p)|FD-Score:-3.14|P-value:8.44E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery Gene:YGL242C(YGL242C_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; deletion mutant is viable Gene:YJL107C(YJL107C_p)|FD-Score:3.37|P-value:3.80E-4||SGD DESC:Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi Gene:YJL163C(YJL163C_p)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Putative protein of unknown function Gene:YJR056C(YJR056C_p)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:YNL097C-B(YNL097C-B_p)|FD-Score:-3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function Gene:YOL046C(YOL046C_d)|FD-Score:3.45|P-value:2.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data;almost completely overlaps the verified gene PSK2/YOL045W Gene:YPR098C(YPR098C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML092C5.305.70E-80.61PRE8Alpha 2 subunit of the 20S proteasome
YLR166C4.691.38E-60.43SEC10Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
YOR048C4.261.03E-50.00RAT1Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination
YOL120C4.261.04E-50.32RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YIL061C3.944.08E-50.35SNP1Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
YIL078W3.591.66E-40.08THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YDR064W3.512.24E-40.02RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YLL037W_d3.492.39E-40.01YLL037W_dDubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
YPL169C3.492.43E-40.19MEX67Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
YER021W3.304.86E-40.07RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YDL148C3.236.20E-40.07NOP14Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
YDL150W3.167.94E-40.14RPC53RNA polymerase III subunit C53
YDL152W_d3.020.001270.07YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YDL120W2.950.001580.07YFH1Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog is mutated in Friedrich's ataxia
YBR236C2.880.001970.01ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPR098C4.201.34E-5YPR098CProtein of unknown function, localized to the mitochondrial outer membrane
YIL157C4.161.61E-5COA1Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
YKR052C4.052.61E-5MRS4Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
YER078C3.904.90E-5ICP55Mitochondrial aminopeptidase; cleaves the N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of the aminopeptidase P family
YNL239W3.894.92E-5LAP3Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
YEL024W3.885.22E-5RIP1Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
YJR080C3.846.08E-5AIM24Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss
YPL196W3.807.13E-5OXR1Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
YPR072W3.778.03E-5NOT5Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation
YCR034W3.711.03E-4FEN1Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication
YEL012W3.701.10E-4UBC8Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
YCR069W3.671.21E-4CPR4Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway
YJL163C_p3.571.81E-4YJL163C_pPutative protein of unknown function
YHR168W3.551.94E-4MTG2Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
YJL199C_d3.462.69E-4MBB1_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies

GO enrichment analysis for SGTC_2263
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0723.82E-8SGTC_30079078383 71.4 μMChembridge (Drug-like library)64969950.186667
0.0664.15E-7SGTC_7181130-0073 349.0 μMChemDiv (Drug-like library)67400200.057971
0.0612.35E-6SGTC_1753793-3120 23.7 μMChemDiv (Drug-like library)53222970.084507
0.0604.26E-6SGTC_1931st074438 57.1 μMTimTec (Natural product derivative library)27873130.121622
0.0588.18E-6SGTC_13702001-0092 44.6 μMChemDiv (Drug-like library)7909570.0606061
0.0579.95E-6SGTC_14153966-0071 78.1 μMChemDiv (Drug-like library)28931850.12963DNA intercalators
0.0525.54E-5SGTC_29959067767 71.4 μMChembridge (Drug-like library)116735670.193548
0.0526.21E-5SGTC_1465k213-0056 144.0 μMChemDiv (Drug-like library)240488570.161765DNA intercalators
0.0491.43E-4SGTC_11951502-0147 41.1 μMChemDiv (Drug-like library)8179400.0952381
0.0491.51E-4SGTC_13401486-1359 257.0 μMChemDiv (Drug-like library)43090680.145161
0.0491.79E-4SGTC_33469158254 43.6 μMChembridge (Drug-like library)176395000.0909091
0.0491.84E-4SGTC_8570438-0306 1.4 μMChemDiv (Drug-like library)46861360.0384615copper-dependent oxidative stress
0.0482.56E-4SGTC_23619034121 116.0 μMChembridge (Fragment library)64969170.0806452RSC complex & mRNA processing
0.0473.03E-4SGTC_14384092-1045 4.0 μMChemDiv (Drug-like library)1351342NA
0.0463.91E-4SGTC_23349017922 200.0 μMChembridge (Fragment library)31631640.027027

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2274794709446.08 μM0.6097562971028Chembridge (Fragment library)246.2851.40323
SGTC_22647948757110.76 μM0.5952382971411Chembridge (Fragment library)252.312721.35624
SGTC_22857945142200 μM0.5319152970608Chembridge (Fragment library)247.273060.25224
SGTC_21745790901200 μM0.458333786020Chembridge (Fragment library)208.237021.28914tubulin folding & SWR complex
SGTC_20635246145200 μM0.444444711240Chembridge (Fragment library)221.639722.67412
SGTC_14494112-4002201 μM0.4363642059539ChemDiv (Drug-like library)331.3183033.1526RPP1 & pyrimidine depletion
SGTC_2860902572538.96 μM0.41071440546248Chembridge (Drug-like library)313.327843.39515RPP1 & pyrimidine depletion
SGTC_20255147754141 μM0.408163315777Chembridge (Fragment library)229.231341.74913
SGTC_21255246583200 μM0.408163835267Chembridge (Fragment library)202.20931.88413tubulin folding & SWR complex
SGTC_22817943571200 μM0.3846151244024Chembridge (Fragment library)231.247222.47913