7929201

1-(6-aminopyridin-2-yl)-3-(2-methylprop-2-enyl)thiourea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2265
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 1265108
SMILES CC(=C)CNC(=S)NC1=CC=CC(=N1)N
Standardized SMILES CC(=C)CNC(=S)Nc1cccc(N)n1
Molecular weight 222.31
ALogP 2.76
H-bond donor count 3
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.01
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1265108
Download HIP data (tab-delimited text)  (excel)
Gene:CCT5(YJR064W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.33||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC34(YDR054C)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CDC9(YDL164C)|FD-Score:-3.13|P-value:8.84E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:INO80(YGL150C)|FD-Score:-3.77|P-value:8.16E-5|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:NNF1(YJR112W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PRP39(YML046W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RER2(YBR002C)|FD-Score:5.66|P-value:7.36E-9|Clearance:0.67||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RNA14(YMR061W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC19(YNL113W)|FD-Score:4.46|P-value:4.00E-6|Clearance:0.11||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL18A(YOL120C)|FD-Score:4|P-value:3.23E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPR2(YIR015W)|FD-Score:7.89|P-value:1.55E-15|Clearance:2.22||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSA4(YCR072C)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:RVB2(YPL235W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.54||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC10(YLR166C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPC98(YNL126W)|FD-Score:4.27|P-value:9.56E-6|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TEN1(YLR010C)|FD-Score:5|P-value:2.92E-7|Clearance:0.53||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:THS1(YIL078W)|FD-Score:4.35|P-value:6.80E-6|Clearance:0.08||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM54(YJL054W)|FD-Score:-5.03|P-value:2.42E-7|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YCS4(YLR272C)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YHR020W(YHR020W)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:CCT5(YJR064W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.33||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CDC34(YDR054C)|FD-Score:-3.14|P-value:8.56E-4|Clearance:0||SGD DESC:Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress Gene:CDC9(YDL164C)|FD-Score:-3.13|P-value:8.84E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:INO80(YGL150C)|FD-Score:-3.77|P-value:8.16E-5|Clearance:0||SGD DESC:ATPase and nucleosome spacing factor, subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro; has a role in modulating stress gene transcription Gene:NNF1(YJR112W)|FD-Score:-3.5|P-value:2.37E-4|Clearance:0||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:PRP39(YML046W)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RER2(YBR002C)|FD-Score:5.66|P-value:7.36E-9|Clearance:0.67||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RNA14(YMR061W)|FD-Score:3.45|P-value:2.84E-4|Clearance:0.05||SGD DESC:Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping Gene:RPC19(YNL113W)|FD-Score:4.46|P-value:4.00E-6|Clearance:0.11||SGD DESC:RNA polymerase subunit AC19, common to RNA polymerases I and III Gene:RPL18A(YOL120C)|FD-Score:4|P-value:3.23E-5|Clearance:0.01||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPR2(YIR015W)|FD-Score:7.89|P-value:1.55E-15|Clearance:2.22||SGD DESC:Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress Gene:RSA4(YCR072C)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:WD-repeat protein involved in ribosome biogenesis; may interact with ribosomes; required for maturation and efficient intra-nuclear transport or pre-60S ribosomal subunits, localizes to the nucleolus Gene:RVB2(YPL235W)|FD-Score:3.99|P-value:3.34E-5|Clearance:0.54||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC10(YLR166C)|FD-Score:-3.24|P-value:5.96E-4|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SPC98(YNL126W)|FD-Score:4.27|P-value:9.56E-6|Clearance:0.28||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TEN1(YLR010C)|FD-Score:5|P-value:2.92E-7|Clearance:0.53||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:THS1(YIL078W)|FD-Score:4.35|P-value:6.80E-6|Clearance:0.08||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TIM54(YJL054W)|FD-Score:-5.03|P-value:2.42E-7|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:YCS4(YLR272C)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:YHR020W(YHR020W)|FD-Score:-3.24|P-value:6.03E-4|Clearance:0||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1265108
Download HOP data (tab-delimited text)  (excel)
Gene:ADA2(YDR448W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE3(YGR204W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM45(YPR004C)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:BCK2(YER167W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CBP1(YJL209W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX14(YML129C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX19(YLL018C-A)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRP1(YHR146W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF4(YPR135W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DBP3(YGL078C)|FD-Score:4|P-value:3.12E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DFG5(YMR238W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DNF1(YER166W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUG1(YFR044C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EXG1(YLR300W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FLC1(YPL221W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP10(YER182W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV6(YNL133C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GEA1(YJR031C)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GRX4(YER174C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:IMP2(YMR035W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IOC3(YFR013W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC6(YFR043C)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:JEN1(YKL217W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MIC17(YMR002W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOG1(YJR074W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NAM2(YLR382C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NIT1(YIL164C)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NOT5(YPR072W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NST1(YNL091W)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTH2(YBR001C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OSW1(YOR255W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PDC6(YGR087C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PDE2(YOR360C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PEX25(YPL112C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PIN2(YOR104W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:RNH201(YNL072W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS16A(YMR143W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SBH1(YER087C-B)|FD-Score:6.63|P-value:1.68E-11||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SGS1(YMR190C)|FD-Score:4.76|P-value:9.91E-7||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHP1(YBL058W)|FD-Score:-3.91|P-value:4.55E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SKP2(YNL311C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SPT7(YBR081C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRB5(YGR104C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSH4(YKL124W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUR2(YDR297W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUS1(YBR111W-A)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SVL3(YPL032C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWI6(YLR182W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SYS1(YJL004C)|FD-Score:-4.29|P-value:8.74E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THP2(YHR167W)|FD-Score:5.26|P-value:7.08E-8||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TOR1(YJR066W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:13.7|P-value:4.55E-43||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAC17(YCL063W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS73(YGL104C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL062W(YBL062W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR242W(YBR242W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDJ1(YNL064C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR344C(YDR344C_d)|FD-Score:-3.77|P-value:8.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:7.24|P-value:2.23E-13||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFT2(YDR319C_p)|FD-Score:-4.74|P-value:1.09E-6||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.85|P-value:6.27E-7||SGD DESC:Putative protein of unknown function Gene:YHK8(YHR048W_p)|FD-Score:4.92|P-value:4.38E-7||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL059C(YIL059C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YKL169C(YKL169C_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR290C(YLR290C_p)|FD-Score:-4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML002W(YML002W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR087W(YMR087W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:4.77|P-value:9.15E-7||SGD DESC:Putative protein of unknown function Gene:YNR071C(YNR071C_p)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR131C(YOR131C_p)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR309C(YOR309C_d)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:10.5|P-value:2.56E-26||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR114W(YPR114W_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YVH1(YIR026C)|FD-Score:9.26|P-value:1.03E-20||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ZRC1(YMR243C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADA2(YDR448W)|FD-Score:4.54|P-value:2.82E-6||SGD DESC:Transcription coactivator, component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes Gene:ADE3(YGR204W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AIM45(YPR004C)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:BCK2(YER167W)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations Gene:CBP1(YJL209W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:3.2|P-value:6.84E-4||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:COX14(YML129C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Mitochondrial membrane protein, involved in translational regulation of Cox1p and assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes Gene:COX19(YLL018C-A)|FD-Score:4.02|P-value:2.85E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRP1(YHR146W)|FD-Score:3.1|P-value:9.76E-4||SGD DESC:Protein that binds to cruciform DNA structures Gene:CTF4(YPR135W)|FD-Score:3.61|P-value:1.55E-4||SGD DESC:Chromatin-associated protein, required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p) and may link DNA synthesis to sister chromatid cohesion Gene:DBP3(YGL078C)|FD-Score:4|P-value:3.12E-5||SGD DESC:RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype Gene:DFG5(YMR238W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DNF1(YER166W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase Gene:DUG1(YFR044C)|FD-Score:4.19|P-value:1.37E-5||SGD DESC:Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p) Gene:EXG1(YLR300W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes Gene:FLC1(YPL221W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance Gene:FMP10(YER182W_p)|FD-Score:-3.12|P-value:9.17E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FYV6(YNL133C)|FD-Score:3.33|P-value:4.31E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining Gene:GEA1(YJR031C)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GRX4(YER174C)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:IMP2(YMR035W)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:IOC3(YFR013W)|FD-Score:3.18|P-value:7.40E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC6(YFR043C)|FD-Score:4.89|P-value:4.96E-7||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:JEN1(YKL217W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:MIC17(YMR002W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress Gene:MOG1(YJR074W)|FD-Score:3.51|P-value:2.23E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:NAM2(YLR382C)|FD-Score:3.46|P-value:2.71E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NIT1(YIL164C)|FD-Score:-3.17|P-value:7.53E-4||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NOT5(YPR072W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NST1(YNL091W)|FD-Score:4.86|P-value:5.99E-7||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NTH2(YBR001C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OSW1(YOR255W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PDC6(YGR087C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PDE2(YOR360C)|FD-Score:3.41|P-value:3.21E-4||SGD DESC:High-affinity cyclic AMP phosphodiesterase, component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon Gene:PEX25(YPL112C)|FD-Score:-3.37|P-value:3.69E-4||SGD DESC:Peripheral peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with homologous protein Pex27p Gene:PIN2(YOR104W)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:RNH201(YNL072W)|FD-Score:3.3|P-value:4.78E-4||SGD DESC:Ribonuclease H2 catalytic subunit, removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome Gene:RPS16A(YMR143W)|FD-Score:3.15|P-value:8.30E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication Gene:RPS21A(YKR057W)|FD-Score:5.07|P-value:1.96E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication Gene:RTG1(YOL067C)|FD-Score:-3.62|P-value:1.46E-4||SGD DESC:Transcription factor (bHLH) involved in interorganelle communication between mitochondria, peroxisomes, and nucleus Gene:SBH1(YER087C-B)|FD-Score:6.63|P-value:1.68E-11||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SGS1(YMR190C)|FD-Score:4.76|P-value:9.91E-7||SGD DESC:Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress Gene:SHP1(YBL058W)|FD-Score:-3.91|P-value:4.55E-5||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SKP2(YNL311C)|FD-Score:-3.27|P-value:5.38E-4||SGD DESC:F-box protein of unknown function predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments Gene:SPT7(YBR081C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Subunit of the SAGA transcriptional regulatory complex, involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex Gene:SRB5(YGR104C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSH4(YKL124W)|FD-Score:-3.3|P-value:4.91E-4||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:SUR2(YDR297W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Sphinganine C4-hydroxylase, catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis Gene:SUS1(YBR111W-A)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP Gene:SVL3(YPL032C)|FD-Score:3.11|P-value:9.44E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:SWI6(YLR182W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:SYS1(YJL004C)|FD-Score:-4.29|P-value:8.74E-6||SGD DESC:Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation Gene:THP2(YHR167W)|FD-Score:5.26|P-value:7.08E-8||SGD DESC:Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance Gene:TOR1(YJR066W)|FD-Score:-3.27|P-value:5.34E-4||SGD DESC:PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication Gene:TPM1(YNL079C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication Gene:TSC3(YBR058C-A)|FD-Score:13.7|P-value:4.55E-43||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.14|P-value:8.47E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:VAC17(YCL063W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS73(YGL104C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family Gene:YBL062W(YBL062W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR242W(YBR242W_p)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YDJ1(YNL064C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family Gene:YDR344C(YDR344C_d)|FD-Score:-3.77|P-value:8.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER130C(YER130C_p)|FD-Score:7.24|P-value:2.23E-13||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YFT2(YDR319C_p)|FD-Score:-4.74|P-value:1.09E-6||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.85|P-value:6.27E-7||SGD DESC:Putative protein of unknown function Gene:YHK8(YHR048W_p)|FD-Score:4.92|P-value:4.38E-7||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YIL059C(YIL059C_d)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W Gene:YKL169C(YKL169C_d)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38 Gene:YLR290C(YLR290C_p)|FD-Score:-4.61|P-value:1.98E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR290C is not an essential gene Gene:YLR455W(YLR455W_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML002W(YML002W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YMR087W(YMR087W)|FD-Score:3.09|P-value:0.00100||SGD DESC:Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Putative protein of unknown function Gene:YMR265C(YMR265C_p)|FD-Score:4.77|P-value:9.15E-7||SGD DESC:Putative protein of unknown function Gene:YNR071C(YNR071C_p)|FD-Score:-3.31|P-value:4.61E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR131C(YOR131C_p)|FD-Score:-4.71|P-value:1.24E-6||SGD DESC:Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress Gene:YOR200W(YOR200W_d)|FD-Score:3.82|P-value:6.62E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR309C(YOR309C_d)|FD-Score:3.73|P-value:9.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58 Gene:YOR376W-A(YOR376W-A_p)|FD-Score:-3.17|P-value:7.63E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:10.5|P-value:2.56E-26||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR114W(YPR114W_p)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Putative protein of unknown function Gene:YVH1(YIR026C)|FD-Score:9.26|P-value:1.03E-20||SGD DESC:Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases Gene:ZRC1(YMR243C)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIR015W7.891.55E-152.22RPR2Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; protein abundance increases in response to DNA replication stress
YBR002C5.667.36E-90.67RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YLR010C5.002.92E-70.53TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YNL113W4.464.00E-60.12RPC19RNA polymerase subunit AC19, common to RNA polymerases I and III
YIL078W4.356.80E-60.08THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YNL126W4.279.56E-60.28SPC98Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YOL120C4.003.23E-50.01RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YPL235W3.993.34E-50.54RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YMR061W3.452.84E-40.05RNA14Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
YJR064W3.393.46E-40.33CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR280W3.060.001110.02RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YIL021W3.040.001198.44E-4RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YCR035C3.040.001190.02RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YPR133C3.010.001290.03SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YKL172W2.990.001420.03EBP2Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A13.704.55E-43TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YKL126W10.502.56E-26YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YIR026C9.261.03E-20YVH1Protein phosphatase involved in vegetative growth at low temperatures, sporulation, and glycogen accumulation; mutants are defective in 60S ribosome assembly; member of the dual-specificity family of protein phosphatases
YER130C_p7.242.23E-13YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YER087C-B6.631.68E-11SBH1Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p
YHR167W5.267.08E-8THP2Subunit of the THO complex, which connects transcription elongation and mitotic recombination, and of the TREX complex, which is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
YKR057W5.071.96E-7RPS21AProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
YMR035W5.022.60E-7IMP2Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
YHR048W_p4.924.38E-7YHK8_pPresumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
YFR043C4.894.96E-7IRC6Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci
YNL091W4.865.99E-7NST1Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
YGL188C-A_p4.856.27E-7YGL188C-A_pPutative protein of unknown function
YMR265C_p4.779.15E-7YMR265C_pPutative protein of unknown function
YMR190C4.769.91E-7SGS1Nucleolar DNA helicase of the RecQ family; involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; potential role as repressor of a subset of rapamycin responsive genes; rapidly lost in response to rapamycin in Rrd1p-dependent manner; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes; forms nuclear foci upon DNA replication stress
YPR004C4.641.78E-6AIM45Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response

GO enrichment analysis for SGTC_2265
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.2835.17E-109SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0645161TSC3-RPN4
0.2571.42E-89SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.121622TSC3-RPN4
0.2391.22E-77SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0819672TSC3-RPN4
0.2351.02E-74SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.109375TSC3-RPN4
0.2294.56E-71SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0571429TSC3-RPN4
0.2274.65E-70SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.0967742TSC3-RPN4
0.2275.19E-70SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0704225TSC3-RPN4
0.2212.56E-66SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0724638TSC3-RPN4
0.2209.43E-66SGTC_1919st067113 52.3 μMTimTec (Natural product derivative library)12682430.102564TSC3-RPN4
0.2131.50E-61SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0625TSC3-RPN4
0.2121.09E-60SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.102564TSC3-RPN4
0.2112.81E-60SGTC_20925303694 80.7 μMChembridge (Fragment library)19636990.1TSC3-RPN4
0.2031.55E-55SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.155172
0.2002.88E-54SGTC_2740felodipine 17.1 μMMiscellaneous33330.114286
0.1982.42E-53SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0666667TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22717849410118.62 μM0.314815972430Chembridge (Fragment library)233.289542.66623
SGTC_23459052315157.45 μM0.3137266469265Chembridge (Fragment library)193.24561.48922
SGTC_9031134-0042114 μM0.291667701009ChemDiv (Drug-like library)229.300843.75522
SGTC_20034023581200 μM0.28571416641307Chembridge (Fragment library)199.677262.51222cell wall signaling
SGTC_23407987427183.45 μM0.2678572239331Chembridge (Fragment library)225.31062.25623
SGTC_7491000-0129128 μM0.264151708470ChemDiv (Drug-like library)257.310943.15423amide catabolism
SGTC_23327982454138.89 μM0.2542372981748Chembridge (Fragment library)247.316123.15223
SGTC_3182910884949.47 μM0.23728817133045Chembridge (Drug-like library)267.322383.66112RPP1 & pyrimidine depletion
SGTC_3191911035249.47 μM0.23728817122424Chembridge (Drug-like library)267.322383.66112
SGTC_3181488-062967.2 μM0.2333333662576ChemDiv (Drug-like library)263.272462.25815copper-dependent oxidative stress