7929956

N-(5-chloro-1-methyl-2-oxo-3H-indol-3-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2266
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2967153
SMILES CC(=O)NC1C2=C(C=CC(=C2)Cl)N(C1=O)C
Standardized SMILES CN1C(=O)C(NC(=O)C)c2cc(Cl)ccc12
Molecular weight 238.6702
ALogP 0.77
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.01
% growth inhibition (Hom. pool) 7.51


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2967153
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:5.37|P-value:4.03E-8|Clearance:1.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT4(YDL143W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DIS3(YOL021C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.12||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG29(YMR134W_p)|FD-Score:5.67|P-value:7.16E-9|Clearance:1.26||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:IQG1(YPL242C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.01||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KIN28(YDL108W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:PBN1(YCL052C)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.16||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPL10(YLR075W)|FD-Score:-4.9|P-value:4.75E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPO31(YOR116C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SEC31(YDL195W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.08||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:STH1(YIL126W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.13||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUA5(YGL169W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAO3(YIL129C)|FD-Score:-4.47|P-value:3.92E-6|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TEN1(YLR010C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.24||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:UBC1(YDR177W)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.06||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP13(YLR222C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.23||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:ACT1(YFL039C)|FD-Score:5.37|P-value:4.03E-8|Clearance:1.26||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:CCT4(YDL143W)|FD-Score:3.7|P-value:1.08E-4|Clearance:0.01||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:DIS3(YOL021C)|FD-Score:3.1|P-value:9.71E-4|Clearance:0.12||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:ERG29(YMR134W_p)|FD-Score:5.67|P-value:7.16E-9|Clearance:1.26||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:IQG1(YPL242C)|FD-Score:3.11|P-value:9.38E-4|Clearance:0.01||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KIN28(YDL108W)|FD-Score:3.25|P-value:5.69E-4|Clearance:0||SGD DESC:Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters Gene:PBN1(YCL052C)|FD-Score:3.86|P-value:5.62E-5|Clearance:0.16||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:RPL10(YLR075W)|FD-Score:-4.9|P-value:4.75E-7|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPO31(YOR116C)|FD-Score:-3.65|P-value:1.31E-4|Clearance:0||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:SEC31(YDL195W)|FD-Score:3.25|P-value:5.72E-4|Clearance:0.08||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:STH1(YIL126W)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.13||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:SUA5(YGL169W)|FD-Score:3.69|P-value:1.10E-4|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TAO3(YIL129C)|FD-Score:-4.47|P-value:3.92E-6|Clearance:0||SGD DESC:Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p Gene:TEN1(YLR010C)|FD-Score:4.11|P-value:2.01E-5|Clearance:0.24||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:UBC1(YDR177W)|FD-Score:3.17|P-value:7.57E-4|Clearance:0.06||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:UTP13(YLR222C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.23||SGD DESC:Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA Gene:YBR190W(YBR190W_d)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2967153
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM29(YKR074W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG3(YBL082C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ARC18(YLR370C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG22(YCL038C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVO2(YMR068W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BER1(YLR412W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BRR1(YPR057W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CCR4(YAL021C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLB1(YGR108W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLC1(YGR167W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COQ1(YBR003W)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CTR1(YPR124W)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DGR1(YNL130C-A_p)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPB3(YBR278W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUF1(YOL087C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EDC3(YEL015W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FMP43(YGR243W)|FD-Score:7.31|P-value:1.33E-13||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GAL7(YBR018C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GAS1(YMR307W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCS1(YDL226C)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GMC2(YLR445W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GYP6(YJL044C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFI1(YPL254W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOL1(YNR055C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:IRC21(YMR073C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAP122(YGL016W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:LAC1(YKL008C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LDB7(YBL006C)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LGE1(YPL055C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LNP1(YHR192W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LST7(YGR057C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAF1(YDR005C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MCH1(YDL054C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MEI4(YER044C-A)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MGM101(YJR144W)|FD-Score:6.71|P-value:1.00E-11||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOG1(YJR074W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSC7(YHR039C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NAM2(YLR382C)|FD-Score:5.07|P-value:2.03E-7||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OSH3(YHR073W)|FD-Score:-4.77|P-value:9.02E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OSW1(YOR255W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PET10(YKR046C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX21(YGR239C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PRK1(YIL095W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PUT4(YOR348C)|FD-Score:-4.86|P-value:5.91E-7||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD55(YDR076W)|FD-Score:8.09|P-value:2.98E-16||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RIC1(YLR039C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIF1(YBR275C)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPA12(YJR063W)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPE1(YJL121C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:-4.95|P-value:3.65E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTT101(YJL047C)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RUP1(YOR138C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:9.98|P-value:9.75E-24||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH4(YDR178W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SET2(YJL168C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SHE4(YOR035C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLM3(YDL033C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SMM1(YNR015W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SOD1(YJR104C)|FD-Score:7.7|P-value:6.99E-15||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:7.48|P-value:3.81E-14||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO1(YNL012W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SRB5(YGR104C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSH4(YKL124W)|FD-Score:-5.3|P-value:5.69E-8||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:STP1(YDR463W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYC1(YOR179C)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TKL1(YPR074C)|FD-Score:8.11|P-value:2.55E-16||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TPO4(YOR273C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMR1(YHL035C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS21(YOR089C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:-4.89|P-value:4.94E-7||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR284W(YBR284W_p)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR050C(YCR050C_p)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDC1(YPL087W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER158C(YER158C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR035C(YFR035C_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL025C(YIL025C_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR014W(YIR014W_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJL027C(YJL027C_p)|FD-Score:-4.91|P-value:4.53E-7||SGD DESC:Putative protein of unknown function Gene:YJL120W(YJL120W_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR018C(YKR018C_p)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YKR033C(YKR033C_d)|FD-Score:-4.82|P-value:7.29E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YML002W(YML002W_p)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML003W(YML003W_p)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR084W(YMR084W_p)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOL036W(YOL036W_p)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR123C(YPR123C_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:ACE2(YLR131C)|FD-Score:4.12|P-value:1.89E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:4.27|P-value:9.95E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM29(YKR074W)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YKR074W is not an essential gene; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG3(YBL082C)|FD-Score:-3.17|P-value:7.58E-4||SGD DESC:Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins Gene:ARC18(YLR370C)|FD-Score:3.23|P-value:6.11E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ATG22(YCL038C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:AVO2(YMR068W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth Gene:BER1(YLR412W)|FD-Score:3.24|P-value:5.91E-4||SGD DESC:Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene Gene:BRR1(YPR057W)|FD-Score:3.76|P-value:8.46E-5||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:CCR4(YAL021C)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CCS1(YMR038C)|FD-Score:3.09|P-value:9.88E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLB1(YGR108W)|FD-Score:-3.11|P-value:9.30E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome Gene:CLC1(YGR167W)|FD-Score:3.24|P-value:5.95E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:COQ1(YBR003W)|FD-Score:4.46|P-value:4.18E-6||SGD DESC:Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis Gene:CTR1(YPR124W)|FD-Score:5.75|P-value:4.57E-9||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:DAL81(YIR023W)|FD-Score:-4.15|P-value:1.67E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DGR1(YNL130C-A_p)|FD-Score:-4.28|P-value:9.46E-6||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPB3(YBR278W)|FD-Score:3.29|P-value:5.06E-4||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:DUF1(YOL087C)|FD-Score:3.96|P-value:3.79E-5||SGD DESC:Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid Gene:EDC3(YEL015W)|FD-Score:3.41|P-value:3.27E-4||SGD DESC:Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress Gene:ERV14(YGL054C)|FD-Score:3.97|P-value:3.55E-5||SGD DESC:COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication Gene:FEN1(YCR034W)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FMP43(YGR243W)|FD-Score:7.31|P-value:1.33E-13||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:GAL7(YBR018C)|FD-Score:3.23|P-value:6.14E-4||SGD DESC:Galactose-1-phosphate uridyl transferase, synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism Gene:GAS1(YMR307W)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GCS1(YDL226C)|FD-Score:5.54|P-value:1.53E-8||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:GMC2(YLR445W)|FD-Score:3.47|P-value:2.56E-4||SGD DESC:Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor Gene:GYP6(YJL044C)|FD-Score:3.42|P-value:3.09E-4||SGD DESC:GTPase-activating protein (GAP) for the yeast Rab family member, Ypt6p; involved in vesicle mediated protein transport Gene:HFI1(YPL254W)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Adaptor protein required for structural integrity of the SAGA complex, a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions Gene:HOL1(YNR055C)|FD-Score:4.47|P-value:3.95E-6||SGD DESC:Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake Gene:IRC21(YMR073C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:IRS4(YKR019C)|FD-Score:-3.45|P-value:2.75E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KAP122(YGL016W)|FD-Score:-3.15|P-value:8.05E-4||SGD DESC:Karyopherin beta, responsible for import of the Toa1p-Toa2p complex into the nucleus; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance Gene:LAC1(YKL008C)|FD-Score:3.13|P-value:8.61E-4||SGD DESC:Ceramide synthase component, involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p Gene:LDB7(YBL006C)|FD-Score:-4.56|P-value:2.57E-6||SGD DESC:Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions Gene:LGE1(YPL055C)|FD-Score:-3.48|P-value:2.55E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LNP1(YHR192W_p)|FD-Score:4.49|P-value:3.50E-6||SGD DESC:Lunapark family member involved in ER network formation; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS Gene:LST7(YGR057C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAF1(YDR005C)|FD-Score:3.26|P-value:5.51E-4||SGD DESC:Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions Gene:MCH1(YDL054C)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MEI4(YER044C-A)|FD-Score:3.11|P-value:9.37E-4||SGD DESC:Meiosis-specific protein involved in double-strand break formation during meiotic recombination; required for chromosome synapsis and production of viable spores Gene:MGM101(YJR144W)|FD-Score:6.71|P-value:1.00E-11||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MOG1(YJR074W)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MSC7(YHR039C)|FD-Score:3.42|P-value:3.16E-4||SGD DESC:Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids Gene:NAM2(YLR382C)|FD-Score:5.07|P-value:2.03E-7||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OSH3(YHR073W)|FD-Score:-4.77|P-value:9.02E-7||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OSW1(YOR255W)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PET10(YKR046C)|FD-Score:-3.22|P-value:6.51E-4||SGD DESC:Protein of unknown function that co-purifies with lipid particles; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange Gene:PEX21(YGR239C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; partially redundant with Pex18p; relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:PRK1(YIL095W)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication Gene:PUT4(YOR348C)|FD-Score:-4.86|P-value:5.91E-7||SGD DESC:Proline permease, required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells Gene:RAD55(YDR076W)|FD-Score:8.09|P-value:2.98E-16||SGD DESC:Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p Gene:RIC1(YLR039C)|FD-Score:3.16|P-value:7.87E-4||SGD DESC:Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes Gene:RIF1(YBR275C)|FD-Score:-3.53|P-value:2.12E-4||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RPA12(YJR063W)|FD-Score:4.3|P-value:8.64E-6||SGD DESC:RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex Gene:RPE1(YJL121C)|FD-Score:5.08|P-value:1.93E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPL34B(YIL052C)|FD-Score:-4.95|P-value:3.65E-7||SGD DESC:Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication Gene:RSC2(YLR357W)|FD-Score:4.13|P-value:1.85E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance Gene:RTT101(YJL047C)|FD-Score:3.74|P-value:9.03E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:RUP1(YOR138C)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:9.98|P-value:9.75E-24||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SDH4(YDR178W)|FD-Score:-3.36|P-value:3.92E-4||SGD DESC:Membrane anchor subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain Gene:SET2(YJL168C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SHE4(YOR035C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Protein containing a UCS (UNC-45/CRO1/SHE4) domain, binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization Gene:SLM3(YDL033C)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF) Gene:SMM1(YNR015W)|FD-Score:-3.29|P-value:4.96E-4||SGD DESC:Dihydrouridine synthase, member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs Gene:SOD1(YJR104C)|FD-Score:7.7|P-value:6.99E-15||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:7.48|P-value:3.81E-14||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO1(YNL012W)|FD-Score:-3.53|P-value:2.06E-4||SGD DESC:Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B Gene:SRB5(YGR104C)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance Gene:SSH4(YKL124W)|FD-Score:-5.3|P-value:5.69E-8||SGD DESC:Specificity factor required for Rsp5p-dependent ubiquitination and sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases Gene:STP1(YDR463W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR4(YLR372W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:4|P-value:3.11E-5||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SYC1(YOR179C)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication Gene:TAT1(YBR069C)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TDA5(YLR426W_p)|FD-Score:5.04|P-value:2.35E-7||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TKL1(YPR074C)|FD-Score:8.11|P-value:2.55E-16||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TLG2(YOL018C)|FD-Score:4.02|P-value:2.93E-5||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TMA46(YOR091W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TPO4(YOR273C)|FD-Score:4.52|P-value:3.15E-6||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VMR1(YHL035C)|FD-Score:3.94|P-value:4.09E-5||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:VPS21(YOR089C)|FD-Score:3.92|P-value:4.46E-5||SGD DESC:Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication Gene:YBR238C(YBR238C)|FD-Score:-4.89|P-value:4.94E-7||SGD DESC:Mitochondrial membrane protein with similarity to Rmd9p; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span Gene:YBR284W(YBR284W_p)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR050C(YCR050C_p)|FD-Score:3.88|P-value:5.30E-5||SGD DESC:Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein Gene:YDC1(YPL087W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication Gene:YDR010C(YDR010C_d)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER076C(YER076C_p)|FD-Score:3.74|P-value:9.27E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization Gene:YER158C(YER158C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication Gene:YFR035C(YFR035C_p)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YIL025C(YIL025C_d)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIR014W(YIR014W_p)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene Gene:YJL027C(YJL027C_p)|FD-Score:-4.91|P-value:4.53E-7||SGD DESC:Putative protein of unknown function Gene:YJL120W(YJL120W_d)|FD-Score:3.99|P-value:3.30E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKR018C(YKR018C_p)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YKR033C(YKR033C_d)|FD-Score:-4.82|P-value:7.29E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YML002W(YML002W_p)|FD-Score:5.96|P-value:1.23E-9||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YML003W(YML003W_p)|FD-Score:3.49|P-value:2.41E-4||SGD DESC:Putative protein of unknown function Gene:YML007C-A(YML007C-A_p)|FD-Score:-3.16|P-value:7.93E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR084W(YMR084W_p)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOL036W(YOL036W_p)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YPR039W(YPR039W_d)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W Gene:YPR123C(YPR123C_d)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YMR134W_p5.677.16E-91.26ERG29_pProtein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
YFL039C5.374.03E-81.26ACT1Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions
YLR010C4.112.01E-50.24TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YCL052C3.865.62E-50.16PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YDL143W3.701.08E-40.01CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL169W3.691.10E-40.07SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YLR222C3.621.48E-40.23UTP13Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YIL126W3.383.56E-40.13STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YDL108W3.255.69E-40.00KIN28Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
YDL195W3.255.72E-40.08SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YDR177W3.177.57E-40.06UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YPL242C3.119.38E-40.01IQG1Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
YOL021C3.109.71E-40.12DIS3Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress
YKR068C2.980.001460.06BET3Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
YDL103C2.920.001770.01QRI1UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR129C9.989.75E-24SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YPR074C8.112.55E-16TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YDR076W8.092.98E-16RAD55Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
YJR104C7.706.99E-15SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YHR008C7.483.81E-14SOD2Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated
YGR243W7.311.33E-13FMP43Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress
YJR144W6.711.00E-11MGM101Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage
YML002W_p5.961.23E-9YML002W_pPutative protein of unknown function; expression induced by heat and by calcium shortage
YPR124W5.754.57E-9CTR1High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress
YDL226C5.541.53E-8GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
YOR179C5.111.62E-7SYC1Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
YJL121C5.081.93E-7RPE1D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
YLR382C5.072.03E-7NAM2Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance
YLR426W_p5.042.35E-7TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YMR068W4.671.51E-6AVO2Component of a complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth

GO enrichment analysis for SGTC_2266
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1782.91E-43SGTC_1060092-0003 11.6 μMChemDiv (Drug-like library)10721480.157895copper-dependent oxidative stress
0.1695.00E-39SGTC_394menadione 8.3 μMMiscellaneous40550.12superoxide
0.1696.73E-39SGTC_2681menadione 3.2 μMMiscellaneous40550.12superoxide
0.1661.27E-37SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.160714superoxide
0.1561.37E-33SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.107143superoxide
0.1561.72E-33SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.113208copper-dependent oxidative stress
0.1397.74E-27SGTC_162k030-0015 37.8 μMChemDiv (Drug-like library)18099200.0925926iron homeostasis
0.1382.06E-26SGTC_13001094-0077 113.0 μMChemDiv (Drug-like library)55326360.220588
0.1233.25E-21SGTC_23985228213 48.8 μMMiscellaneous807710.156863iron homeostasis
0.1226.12E-21SGTC_7991310-0557 151.0 μMChemDiv (Drug-like library)53544920.112903superoxide
0.1219.02E-21SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.126984RPP1 & pyrimidine depletion
0.1195.99E-20SGTC_21966573537 42.7 μMChembridge (Fragment library)29030870.12069copper-dependent oxidative stress
0.1163.29E-19SGTC_23569061756 189.0 μMChembridge (Fragment library)201040430.37037
0.1141.81E-18SGTC_2671danthron 100.0 μMMicrosource (Natural product library)29500.1DNA damage response
0.1134.04E-18SGTC_1000086-0128 49.6 μMChemDiv (Drug-like library)932970.131148copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2945906768161.76 μM0.39655216456930Chembridge (Drug-like library)344.792182.40113
SGTC_2001402350257.66 μM0.386364108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_23569061756188.97 μM0.3703720104043Chembridge (Fragment library)246.304921.46312
SGTC_2919797818846.38 μM0.3114752980291Chembridge (Drug-like library)331.750362.26224
SGTC_23369001819198.65 μM0.3061222989602Chembridge (Fragment library)209.629021.88902heme biosynthesis & mitochondrial translocase
SGTC_297190921869.93 μM0.325236400Chembridge (Drug-like library)343.271343.6313ergosterol biosynthesis
SGTC_22677931562200 μM0.290909934880Chembridge (Fragment library)267.754521.79313
SGTC_2377907335162.6 μM0.2909097337946Chembridge (Fragment library)281.78112.25613ergosterol biosynthesis
SGTC_1741st03813410.23 μM0.2807021272587TimTec (Natural product derivative library)393.17774.13905
SGTC_2376907292281.32 μM0.28070217087968Chembridge (Fragment library)281.78112.2513