7947094

2-benzamidothiophene-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2274
Screen concentration 46.1 μM
Source Chembridge (Fragment library)
PubChem CID 2971028
SMILES C1=CC=C(C=C1)C(=O)NC2=C(C=CS2)C(=O)N
Standardized SMILES NC(=O)c1ccsc1NC(=O)c2ccccc2
Molecular weight 246.285
ALogP 1.4
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 8.53
% growth inhibition (Hom. pool) 6.47


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2971028
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG14(YBR070C)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CCT5(YJR064W)|FD-Score:3.95|P-value:3.88E-5|Clearance:0.18||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:4.36|P-value:6.39E-6|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.14||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC53(YDL132W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CDC8(YJR057W)|FD-Score:5.13|P-value:1.42E-7|Clearance:0.34||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:ECM9(YKR004C)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:ERG8(YMR220W)|FD-Score:-3.81|P-value:7.02E-5|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MAS1(YLR163C)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.16||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:POP4(YBR257W)|FD-Score:7.12|P-value:5.59E-13|Clearance:1.98||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:REB1(YBR049C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPC53(YDL150W)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:SEC10(YLR166C)|FD-Score:-6.32|P-value:1.34E-10|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEN15(YMR059W)|FD-Score:-3.74|P-value:9.26E-5|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SUA5(YGL169W)|FD-Score:4.43|P-value:4.68E-6|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TEN1(YLR010C)|FD-Score:4.8|P-value:8.11E-7|Clearance:0.36||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFC6(YDR362C)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDL152W(YDL152W_d)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:ACT1(YFL039C)|FD-Score:-3.36|P-value:3.84E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:ALG14(YBR070C)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CCT5(YJR064W)|FD-Score:3.95|P-value:3.88E-5|Clearance:0.18||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:4.36|P-value:6.39E-6|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC20(YGL116W)|FD-Score:3.47|P-value:2.59E-4|Clearance:0.14||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC53(YDL132W)|FD-Score:3.6|P-value:1.61E-4|Clearance:0.06||SGD DESC:Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation Gene:CDC8(YJR057W)|FD-Score:5.13|P-value:1.42E-7|Clearance:0.34||SGD DESC:Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p Gene:ECM9(YKR004C)|FD-Score:-3.54|P-value:2.00E-4|Clearance:0||SGD DESC:Non-essential protein of unknown function Gene:ERG8(YMR220W)|FD-Score:-3.81|P-value:7.02E-5|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:EXO70(YJL085W)|FD-Score:-3.12|P-value:9.05E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:GLC7(YER133W)|FD-Score:3.51|P-value:2.21E-4|Clearance:0.04||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MAS1(YLR163C)|FD-Score:3.76|P-value:8.65E-5|Clearance:0.16||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:POP4(YBR257W)|FD-Score:7.12|P-value:5.59E-13|Clearance:1.98||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:REB1(YBR049C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.02||SGD DESC:RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication Gene:RPC53(YDL150W)|FD-Score:3.78|P-value:7.96E-5|Clearance:0.02||SGD DESC:RNA polymerase III subunit C53 Gene:SEC10(YLR166C)|FD-Score:-6.32|P-value:1.34E-10|Clearance:0||SGD DESC:Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion Gene:SEN15(YMR059W)|FD-Score:-3.74|P-value:9.26E-5|Clearance:0||SGD DESC:Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p Gene:SUA5(YGL169W)|FD-Score:4.43|P-value:4.68E-6|Clearance:0.07||SGD DESC:Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family Gene:TEN1(YLR010C)|FD-Score:4.8|P-value:8.11E-7|Clearance:0.36||SGD DESC:Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p Gene:TFC6(YDR362C)|FD-Score:-3.15|P-value:8.30E-4|Clearance:0||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110 Gene:YDL152W(YDL152W_d)|FD-Score:3.33|P-value:4.35E-4|Clearance:0.27||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2971028
Download HOP data (tab-delimited text)  (excel)
Gene:API2(YDR525W_d)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATO2(YNR002C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.89|P-value:5.14E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD14(YAR014C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC73(YLR418C)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CHS5(YLR330W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COS1(YNL336W)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX19(YLL018C-A)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRP1(YHR146W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Protein that binds to cruciform DNA structures Gene:CWC27(YPL064C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DBF20(YPR111W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DGR1(YNL130C-A_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DID4(YKL002W)|FD-Score:-5.07|P-value:1.99E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECI1(YLR284C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:FIG4(YNL325C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FPS1(YLL043W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FUM1(YPL262W)|FD-Score:-6.36|P-value:1.02E-10||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCN4(YEL009C)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLK1(YCL040W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HOF1(YMR032W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IMG1(YCR046C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC3(YFR013W)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC13(YOR235W_d)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH2(YOL002C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:IZH4(YOL101C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KCS1(YDR017C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KTR6(YPL053C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LIP2(YLR239C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAG2(YLR427W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MCH4(YOL119C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPS8(YMR158W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS18(YPR134W)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC7(YEL033W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUM3(YOR298W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NEM1(YHR004C)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NOP16(YER002W)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPP1(YCR026C)|FD-Score:5.59|P-value:1.17E-8||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPT1(YOR209C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OST3(YOR085W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PCI8(YIL071C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCP1(YGR101W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR10(YOR328W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PDR16(YNL231C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHA2(YNL316C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO80(YOL001W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PIN2(YOR104W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:POF1(YCL047C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRM4(YPL156C)|FD-Score:-5.98|P-value:1.09E-9||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:QCR9(YGR183C)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RDS1(YCR106W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RPL16A(YIL133C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL19B(YBL027W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RRI2(YOL117W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRT1(YBL048W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSC1(YGR056W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT107(YHR154W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RUP1(YOR138C)|FD-Score:6.97|P-value:1.60E-12||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:8.53|P-value:7.57E-18||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SDS3(YIL084C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SGE1(YPR198W)|FD-Score:5.93|P-value:1.48E-9||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLS1(YLR139C)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPO14(YKR031C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPT2(YER161C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA4(YER103W)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TDA5(YLR426W_p)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOM7(YNL070W)|FD-Score:5|P-value:2.85E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRI1(YMR233W)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP6(YFR010W)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UPS2(YLR168C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS25(YJR102C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:WSC2(YNL283C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR242W(YBR242W_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR102C(YCR102C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR010C(YDR010C_d)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR053C(YGR053C_p)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:4.93|P-value:4.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL182C(YJL182C_d)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR296W(YLR296W_d)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function Gene:YMR206W(YMR206W_p)|FD-Score:-5.74|P-value:4.80E-9||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR209C(YMR209C_p)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:-4.94|P-value:3.85E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR200W(YOR200W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR366W(YOR366W_d)|FD-Score:5|P-value:2.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL229W(YPL229W_p)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Putative protein of unknown function Gene:API2(YDR525W_d)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology Gene:ATO2(YNR002C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication Gene:BCH2(YKR027W)|FD-Score:3.78|P-value:7.99E-5||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:4.89|P-value:5.14E-7||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD14(YAR014C)|FD-Score:4.25|P-value:1.05E-5||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CCM1(YGR150C)|FD-Score:-3.95|P-value:3.86E-5||SGD DESC:Mitochondrial 15s rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport Gene:CDC73(YLR418C)|FD-Score:4.4|P-value:5.34E-6||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CHS5(YLR330W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane Gene:COS1(YNL336W)|FD-Score:-3.12|P-value:8.89E-4||SGD DESC:Protein of unknown function, member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins Gene:COX19(YLL018C-A)|FD-Score:3.42|P-value:3.10E-4||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:CRP1(YHR146W)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:Protein that binds to cruciform DNA structures Gene:CWC27(YPL064C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress Gene:DBF20(YPR111W)|FD-Score:3.71|P-value:1.04E-4||SGD DESC:Ser/Thr kinase involved in late nuclear division, one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs Gene:DGR1(YNL130C-A_p)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DID4(YKL002W)|FD-Score:-5.07|P-value:1.99E-7||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:ECI1(YLR284C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced Gene:FIG4(YNL325C)|FD-Score:4.22|P-value:1.23E-5||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FPS1(YLL043W)|FD-Score:4.22|P-value:1.24E-5||SGD DESC:Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress Gene:FUM1(YPL262W)|FD-Score:-6.36|P-value:1.02E-10||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:GCN4(YEL009C)|FD-Score:-4.47|P-value:3.99E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GDB1(YPR184W)|FD-Score:-3.39|P-value:3.52E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GET3(YDL100C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress Gene:GLE2(YER107C)|FD-Score:3.17|P-value:7.50E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GLK1(YCL040W)|FD-Score:-3.33|P-value:4.39E-4||SGD DESC:Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication Gene:GNT1(YOR320C)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:N-acetylglucosaminyltransferase capable of modification of N-linked glycans in the Golgi apparatus Gene:HOF1(YMR032W)|FD-Score:4.51|P-value:3.21E-6||SGD DESC:Bud neck-localized, SH3 domain-containing protein required for cytokinesis; is phosphorylated by Dbf2p; regulates actomyosin ring dynamics and septin localization; interacts with the formins, Bni1p and Bnr1p, and with Cyk3p, Vrp1p, and Bni5p Gene:IMG1(YCR046C)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome Gene:IOC3(YFR013W)|FD-Score:4.36|P-value:6.38E-6||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC13(YOR235W_d)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays increased levels of spontaneous Rad52 foci Gene:IZH2(YOL002C)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc Gene:IZH4(YOL101C)|FD-Score:4.08|P-value:2.26E-5||SGD DESC:Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress Gene:KCS1(YDR017C)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Inositol hexakisphosphate (IP6) and inositol heptakisphosphate (IP7) kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response and telomere maintenance Gene:KTR6(YPL053C)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LCB5(YLR260W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:LIP2(YLR239C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups Gene:MAG2(YLR427W)|FD-Score:3.53|P-value:2.12E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MCH4(YOL119C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport Gene:MRPS8(YMR158W)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSS18(YPR134W)|FD-Score:4.35|P-value:6.79E-6||SGD DESC:Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions Gene:MTC7(YEL033W_p)|FD-Score:3.59|P-value:1.68E-4||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MUM3(YOR298W)|FD-Score:3.09|P-value:9.85E-4||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NEM1(YHR004C)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard Gene:NOP16(YER002W)|FD-Score:-4.15|P-value:1.64E-5||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:NPP1(YCR026C)|FD-Score:5.59|P-value:1.17E-8||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NPT1(YOR209C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus Gene:OST3(YOR085W)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Gene:PCI8(YIL071C)|FD-Score:3.46|P-value:2.74E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PCP1(YGR101W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Mitochondrial serine protease required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases Gene:PDR10(YOR328W)|FD-Score:-3.3|P-value:4.86E-4||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p Gene:PDR16(YNL231C)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress Gene:PFK2(YMR205C)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:PHA2(YNL316C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Prephenate dehydratase, catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway Gene:PHO80(YOL001W)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling Gene:PIN2(YOR104W)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Protein that induces appearance of [PIN+] prion when overproduced; predicted to be palmitoylated Gene:POF1(YCL047C)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:ATPase involved in the protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion; also interacts with Ubc7p Gene:PRM4(YPL156C)|FD-Score:-5.98|P-value:1.09E-9||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:QCR9(YGR183C)|FD-Score:-3.91|P-value:4.57E-5||SGD DESC:Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex Gene:RDS1(YCR106W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide Gene:RPL16A(YIL133C)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL19B(YBL027W)|FD-Score:3.76|P-value:8.60E-5||SGD DESC:Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication Gene:RQC1(YDR333C_p)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Ltn1p-Tae2p-Cdc48p-Npl4p-Ufd1p), is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Ltn1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC Gene:RRI2(YOL117W)|FD-Score:3.26|P-value:5.59E-4||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RRT1(YBL048W_d)|FD-Score:3.36|P-value:3.96E-4||SGD DESC:Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:RSC1(YGR056W)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:RTT107(YHR154W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:RUP1(YOR138C)|FD-Score:6.97|P-value:1.60E-12||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:SAC6(YDR129C)|FD-Score:8.53|P-value:7.57E-18||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SAP1(YER047C)|FD-Score:3.11|P-value:9.38E-4||SGD DESC:Putative ATPase of the AAA family, interacts with the Sin1p transcriptional repressor in the two-hybrid system Gene:SDS3(YIL084C)|FD-Score:3.88|P-value:5.12E-5||SGD DESC:Component of the Rpd3p/Sin3p deacetylase complex required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; cells defective in SDS3 display pleiotropic phenotypes Gene:SGE1(YPR198W)|FD-Score:5.93|P-value:1.48E-9||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SLS1(YLR139C)|FD-Score:4.41|P-value:5.21E-6||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SPO14(YKR031C)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions Gene:SPT2(YER161C)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components, has similarity to mammalian HMG1 proteins Gene:SSA4(YER103W)|FD-Score:5.41|P-value:3.22E-8||SGD DESC:Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication Gene:SUL1(YBR294W)|FD-Score:-3.9|P-value:4.80E-5||SGD DESC:High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates Gene:TDA5(YLR426W_p)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele Gene:TOM7(YNL070W)|FD-Score:5|P-value:2.85E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:TRI1(YMR233W)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:-3.49|P-value:2.37E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UBP6(YFR010W)|FD-Score:4.6|P-value:2.11E-6||SGD DESC:Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance Gene:UPS2(YLR168C)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Mitochondrial protein involved in phospholipid metabolism; localizes to the intermembrane space; has a role in regulation of phospholipid metabolism by inhibiting conversion of phosphatidylethanolamine to phosphatidylcholine; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI Gene:VPS25(YJR102C)|FD-Score:3.17|P-value:7.62E-4||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:WSC2(YNL283C)|FD-Score:3.88|P-value:5.13E-5||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR242W(YBR242W_p)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR242W is not an essential gene Gene:YCR061W(YCR061W_p)|FD-Score:5.57|P-value:1.30E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR102C(YCR102C_p)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Putative protein of unknown function, involved in copper metabolism; similar to C. carbonum toxD gene; member of the quinone oxidoreductase family Gene:YDR010C(YDR010C_d)|FD-Score:4.38|P-value:6.01E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR169C-A(YDR169C-A_p)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YGR053C(YGR053C_p)|FD-Score:4.1|P-value:2.04E-5||SGD DESC:Putative protein of unknown function Gene:YHR125W(YHR125W_d)|FD-Score:4.93|P-value:4.09E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL134C-A(YIL134C-A_p)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL182C(YJL182C_d)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W Gene:YLR296W(YLR296W_d)|FD-Score:-3.32|P-value:4.55E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML096W(YML096W_p)|FD-Score:3.09|P-value:9.91E-4||SGD DESC:Putative protein of unknown function with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10 Gene:YMR001C-A(YMR001C-A_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Putative protein of unknown function Gene:YMR206W(YMR206W_p)|FD-Score:-5.74|P-value:4.80E-9||SGD DESC:Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication Gene:YMR209C(YMR209C_p)|FD-Score:-3.85|P-value:6.02E-5||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; YMR209C is not an essential gene Gene:YNL058C(YNL058C_p)|FD-Score:-4.94|P-value:3.85E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YNL058C is not an essential gene Gene:YOR200W(YOR200W_d)|FD-Score:3.17|P-value:7.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YOR366W(YOR366W_d)|FD-Score:5|P-value:2.81E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C Gene:YPL229W(YPL229W_p)|FD-Score:3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication Gene:YPR027C(YPR027C_p)|FD-Score:3.4|P-value:3.36E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YBR257W7.125.59E-131.98POP4Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YJR057W5.131.42E-70.34CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YLR010C4.808.11E-70.36TEN1Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
YGL169W4.434.68E-60.07SUA5Single-stranded telomeric DNA-binding protein, required for normal telomere length; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family
YJL111W4.366.39E-60.41CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YJR064W3.953.88E-50.18CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDL150W3.787.96E-50.02RPC53RNA polymerase III subunit C53
YLR163C3.768.65E-50.16MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YDL132W3.601.61E-40.06CDC53Cullin, structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation
YBR049C3.542.02E-40.02REB1RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; REB1 has a paralog, NSI1, that arose from the whole genome duplication
YER133W3.512.21E-40.04GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YGL116W3.472.59E-40.14CDC20Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
YDL152W_d3.334.35E-40.27YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YBR140C3.060.001120.00IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YHR197W3.050.001140.10RIX1Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR129C8.537.57E-18SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YOR138C6.971.60E-12RUP1Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
YPR198W5.931.48E-9SGE1Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
YCR026C5.591.17E-8NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication
YLR426W_p5.571.29E-8TDA5_pPutative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
YCR061W_p5.571.30E-8YCR061W_pProtein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation
YER103W5.413.22E-8SSA4Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
YOR366W_d5.002.81E-7YOR366W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
YNL070W5.002.85E-7TOM7Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
YHR125W_d4.934.09E-7YHR125W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YPL069C4.895.14E-7BTS1Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
YMR233W4.701.31E-6TRI1Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
YHR146W4.671.51E-6CRP1Protein that binds to cruciform DNA structures
YLR284C4.661.58E-6ECI1Peroxisomal delta3,delta2-enoyl-CoA isomerase, hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced
YFR010W4.602.11E-6UBP6Ubiquitin-specific protease situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance

GO enrichment analysis for SGTC_2274
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0984.59E-14SGTC_23579067248 200.0 μMChembridge (Fragment library)91502350.0877193
0.0952.63E-13SGTC_2509methyl 7-deshydroxypyrogallin-4-carboxylate 54.6 μMMicrosource (Natural product library)50988840.1RPP1 & pyrimidine depletion
0.0943.77E-13SGTC_20575236571 38.3 μMChembridge (Fragment library)54684039NARPP1 & pyrimidine depletion
0.0944.60E-13SGTC_2507purpurin 79.7 μMMicrosource (Natural product library)66830.122449copper-dependent oxidative stress
0.0863.43E-11SGTC_21565649887 115.0 μMChembridge (Fragment library)54680440NARPP1 & pyrimidine depletion
0.0841.18E-10SGTC_5394859-0462 2.5 μMChemDiv (Drug-like library)464994740.102941RNA pol III & RNase P/MRP
0.0757.81E-9SGTC_22667929956 200.0 μMChembridge (Fragment library)29671530.118644
0.0757.74E-9SGTC_33559151058 44.0 μMChembridge (Drug-like library)170157280.193548RNA processing & uracil transport
0.0723.08E-8SGTC_22707924575 90.9 μMChembridge (Fragment library)12621350.0689655RPP1 & pyrimidine depletion
0.0707.71E-8SGTC_1872amphotericin b 148.0 nMMicrosource (Natural product library)166676790.0571429RNA pol III & RNase P/MRP
0.0681.85E-7SGTC_31829108849 49.5 μMChembridge (Drug-like library)171330450.277778RPP1 & pyrimidine depletion
0.0672.93E-7SGTC_2597laccaic acid a 100.0 μMMicrosource (Natural product library)54914150.0945946
0.0655.16E-7SGTC_22647948757 110.8 μMChembridge (Fragment library)29714110.657895
0.0656.29E-7SGTC_980074-0023 107.7 μMChemDiv (Drug-like library)35638210.125Golgi
0.0631.46E-6SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.0983607RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22857945142200 μM0.72970608Chembridge (Fragment library)247.273060.25224
SGTC_22647948757110.76 μM0.6578952971411Chembridge (Fragment library)252.312721.35624
SGTC_22637948595193.29 μM0.6097562971371Chembridge (Fragment library)236.247120.79824
SGTC_9272425-1185123 μM0.47619730375ChemDiv (Drug-like library)316.353243.13522
SGTC_22877945887168.79 μM0.3695651245853Chembridge (Fragment library)213.253541.25914
SGTC_22067254556146.97 μM0.3673474398905Chembridge (Fragment library)275.326220.8842460S ribosome export
SGTC_2276794751342.08 μM0.3617022105100Chembridge (Fragment library)234.297442.23314
SGTC_10603448-735082 μM0.355556570178ChemDiv (Drug-like library)237.253342.91812Golgi
SGTC_3108912178549.47 μM0.34545517140271Chembridge (Drug-like library)312.3632.79223
SGTC_23196033012152.6 μM0.333333847246Chembridge (Fragment library)219.262960.86524tubulin folding & SWR complex