| Deletion Strain | FD score | P-value | Gene | Gene Description |
|---|
| YMR058W | 19.70 | 5.90E-87 | FET3 | Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress |
| YDR270W | 12.50 | 2.91E-36 | CCC2 | Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism |
| YJR033C | 11.70 | 8.36E-32 | RAV1 | Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate |
| YJL094C | 10.70 | 4.45E-27 | KHA1 | Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies |
| YER145C | 9.09 | 5.09E-20 | FTR1 | High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress |
| YOR089C | 8.35 | 3.44E-17 | VPS21 | Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication |
| YDL077C | 7.35 | 1.01E-13 | VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p |
| YGR223C | 7.26 | 1.87E-13 | HSV2 | Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization |
| YJL004C | 7.16 | 3.95E-13 | SYS1 | Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation |
| YJR040W | 6.73 | 8.23E-12 | GEF1 | Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism |
| YDR271C_d | 6.03 | 8.31E-10 | YDR271C_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W |
| YDR455C_d | 5.93 | 1.47E-9 | YDR455C_d | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W |
| YNL259C | 5.80 | 3.31E-9 | ATX1 | Cytosolic copper metallochaperone that transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake |
| YDR336W_p | 5.51 | 1.76E-8 | YDR336W_p | Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene |
| YMR123W | 5.49 | 1.97E-8 | PKR1 | V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress |
