7948423

N-(4,5-dihydro-1,3-thiazol-2-yl)-3-methyl-1-benzofuran-2-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2279
Screen concentration 32.9 μM
Source Chembridge (Fragment library)
PubChem CID 1247112
SMILES CC1=C(OC2=CC=CC=C12)C(=O)NC3=NCCS3
Standardized SMILES Cc1c(oc2ccccc12)C(=O)NC3=NCCS3
Molecular weight 260.3116
ALogP 3.18
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.37
% growth inhibition (Hom. pool) 3.65


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1247112
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ERG12(YMR208W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.02||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FIP1(YJR093C)|FD-Score:3.75|P-value:8.93E-5|Clearance:0.22||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:IMP3(YHR148W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.12||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MAS2(YHR024C)|FD-Score:4.77|P-value:9.29E-7|Clearance:0.22||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.2||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MET4(YNL103W)|FD-Score:-3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MGE1(YOR232W)|FD-Score:5.02|P-value:2.62E-7|Clearance:0.25||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NUP57(YGR119C)|FD-Score:-3.3|P-value:4.85E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE8(YML092C)|FD-Score:5.61|P-value:1.02E-8|Clearance:0.59||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAD53(YPL153C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.05||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RFC2(YJR068W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.21||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPL10(YLR075W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.17||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL30(YGL030W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RSC9(YML127W)|FD-Score:-4.4|P-value:5.35E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SCC4(YER147C)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.03||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SLD3(YGL113W)|FD-Score:-3.39|P-value:3.43E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPN1(YPR133C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.06||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:4.55|P-value:2.66E-6|Clearance:0.23||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:UTP21(YLR409C)|FD-Score:4.13|P-value:1.82E-5|Clearance:0.14||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YDR413C(YDR413C_d)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing Gene:ARC19(YKL013C)|FD-Score:-3.18|P-value:7.41E-4|Clearance:0||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ERG12(YMR208W)|FD-Score:3.17|P-value:7.52E-4|Clearance:0.02||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FIP1(YJR093C)|FD-Score:3.75|P-value:8.93E-5|Clearance:0.22||SGD DESC:Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p Gene:IMP3(YHR148W)|FD-Score:3.53|P-value:2.07E-4|Clearance:0.12||SGD DESC:Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA Gene:MAS2(YHR024C)|FD-Score:4.77|P-value:9.29E-7|Clearance:0.22||SGD DESC:Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MEC1(YBR136W)|FD-Score:4.33|P-value:7.56E-6|Clearance:0.2||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:MET4(YNL103W)|FD-Score:-3.22|P-value:6.49E-4|Clearance:0||SGD DESC:Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p Gene:MGE1(YOR232W)|FD-Score:5.02|P-value:2.62E-7|Clearance:0.25||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NUP57(YGR119C)|FD-Score:-3.3|P-value:4.85E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup49p) Gene:PRE8(YML092C)|FD-Score:5.61|P-value:1.02E-8|Clearance:0.59||SGD DESC:Alpha 2 subunit of the 20S proteasome Gene:RAD53(YPL153C)|FD-Score:3.16|P-value:7.95E-4|Clearance:0.05||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RFC2(YJR068W)|FD-Score:3.98|P-value:3.39E-5|Clearance:0.21||SGD DESC:Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon Gene:RPL10(YLR075W)|FD-Score:3.41|P-value:3.29E-4|Clearance:0.17||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RPL30(YGL030W)|FD-Score:-3.96|P-value:3.80E-5|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L30; involved in pre-rRNA processing in the nucleolus; autoregulates splicing of its transcript; homologous to mammalian ribosomal protein L30, no bacterial homolog Gene:RSC9(YML127W)|FD-Score:-4.4|P-value:5.35E-6|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SCC4(YER147C)|FD-Score:3.78|P-value:7.90E-5|Clearance:0.03||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SLD3(YGL113W)|FD-Score:-3.39|P-value:3.43E-4|Clearance:0||SGD DESC:Protein involved in the initiation of DNA replication; required for proper assembly of replication proteins at the origins of replication; interacts with Cdc45p; localizes to nuclear foci that become diffuse upon DNA replication stress Gene:SPN1(YPR133C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.06||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SPT6(YGR116W)|FD-Score:4.55|P-value:2.66E-6|Clearance:0.23||SGD DESC:Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region Gene:UTP21(YLR409C)|FD-Score:4.13|P-value:1.82E-5|Clearance:0.14||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YDR413C(YDR413C_d)|FD-Score:3.11|P-value:9.45E-4|Clearance:0.12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1247112
Download HOP data (tab-delimited text)  (excel)
Gene:AIM45(YPR004C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARG81(YML099C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASP1(YDR321W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:ATH1(YPR026W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:BEM4(YPL161C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIO5(YNR056C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUD21(YOR078W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CPR4(YCR069W)|FD-Score:-7.61|P-value:1.33E-14||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRG1(YHR209W)|FD-Score:7.71|P-value:6.50E-15||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CTR9(YOL145C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:EDS1(YBR033W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:FAR1(YJL157C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FUM1(YPL262W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:FUN30(YAL019W)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:HOR7(YMR251W-A)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HTA2(YBL003C)|FD-Score:5.53|P-value:1.61E-8||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HXK2(YGL253W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ISC10(YER180C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KNH1(YDL049C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MAK31(YCR020C-A)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MGM101(YJR144W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MON1(YGL124C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRI1(YPR118W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRP17(YKL003C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MTQ1(YNL063W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NOT5(YPR072W)|FD-Score:-4.31|P-value:8.34E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPP1(YCR026C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NTH2(YBR001C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OSH3(YHR073W)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OXR1(YPL196W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEX5(YDR244W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PFK2(YMR205C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POL4(YCR014C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:RAD33(YML011C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REI1(YBR267W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:REV1(YOR346W)|FD-Score:5.36|P-value:4.27E-8||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGA2(YDR379W)|FD-Score:6.11|P-value:5.07E-10||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RPL7A(YGL076C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPL9B(YNL067W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPN4(YDL020C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT101(YJL047C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCP160(YJL080C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SFT2(YBL102W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHP1(YBL058W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIS2(YKR072C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX1(YBR228W)|FD-Score:-5.34|P-value:4.73E-8||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPS4(YOR313C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SWC5(YBR231C)|FD-Score:-4.76|P-value:9.81E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TEL1(YBL088C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:VAC7(YNL054W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAM3(YOR106W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VMA13(YPR036W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS27(YNR006W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS35(YJL154C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YAF9(YNL107W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR101C(YCR101C_p)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YER135C(YER135C_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR056C(YFR056C_d)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YIL102C(YIL102C_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function Gene:YJR018W(YJR018W_d)|FD-Score:-6.41|P-value:7.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:4.29|P-value:8.82E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR312C(YLR312C_p)|FD-Score:-4.6|P-value:2.15E-6||SGD DESC:Putative protein of unknown function Gene:YML002W(YML002W_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Putative protein of unknown function Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZRC1(YMR243C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:AIM45(YPR004C)|FD-Score:-3.41|P-value:3.29E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:ARG81(YML099C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ASP1(YDR321W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:ATH1(YPR026W)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Acid trehalase required for utilization of extracellular trehalose Gene:BEM4(YPL161C)|FD-Score:-3.16|P-value:7.99E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BIO5(YNR056C)|FD-Score:4.2|P-value:1.34E-5||SGD DESC:Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis Gene:BUD21(YOR078W)|FD-Score:-3.28|P-value:5.26E-4||SGD DESC:Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern Gene:CPR4(YCR069W)|FD-Score:-7.61|P-value:1.33E-14||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway Gene:CRG1(YHR209W)|FD-Score:7.71|P-value:6.50E-15||SGD DESC:S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin Gene:CTR9(YOL145C)|FD-Score:-4.16|P-value:1.60E-5||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:EDS1(YBR033W_p)|FD-Score:3.65|P-value:1.29E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:FAR1(YJL157C)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Cyclin-dependent kinase inhibitor; mediates cell cycle arrest in response to pheromone; also forms a complex with Cdc24p, Ste4p, and Ste18p that may specify the direction of polarized growth during mating; potential Cdc28p substrate; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:FEN1(YCR034W)|FD-Score:3.15|P-value:8.26E-4||SGD DESC:Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; FEN1 has a paralog, ELO1, that arose from the whole genome duplication Gene:FIG4(YNL325C)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain Gene:FUM1(YPL262W)|FD-Score:4.33|P-value:7.57E-6||SGD DESC:Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria Gene:FUN30(YAL019W)|FD-Score:-3.79|P-value:7.58E-5||SGD DESC:Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate Gene:HOR7(YMR251W-A)|FD-Score:-3.14|P-value:8.48E-4||SGD DESC:Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication Gene:HSV2(YGR223C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization Gene:HTA2(YBL003C)|FD-Score:5.53|P-value:1.61E-8||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:HXK2(YGL253W)|FD-Score:3.22|P-value:6.45E-4||SGD DESC:Hexokinase isoenzyme 2; catalyzes phosphorylation of glucose in the cytosol; predominant hexokinase during growth on glucose; functions in the nucleus to repress expression of HXK1 and GLK1 and to induce expression of its own gene; phosphorylation/dephosphorylation at serine-14 by protein kinase Snf1p and protein phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; HXK2 has a paralog, HXK1, that arose from the whole genome duplication Gene:HYR1(YIR037W)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Thiol peroxidase that functions as a hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce a redox signal to the Yap1p transcription factor Gene:ISC10(YER180C)|FD-Score:3.43|P-value:2.97E-4||SGD DESC:Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells Gene:KNH1(YDL049C)|FD-Score:3.31|P-value:4.72E-4||SGD DESC:Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance Gene:MAK31(YCR020C-A)|FD-Score:-3.71|P-value:1.02E-4||SGD DESC:Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family Gene:MGM101(YJR144W)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage Gene:MON1(YGL124C)|FD-Score:-3.47|P-value:2.56E-4||SGD DESC:Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate Gene:MRI1(YPR118W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:MRP17(YKL003C)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator Gene:MTQ1(YNL063W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NOT5(YPR072W)|FD-Score:-4.31|P-value:8.34E-6||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NPP1(YCR026C)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NTH2(YBR001C)|FD-Score:3.3|P-value:4.88E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OSH3(YHR073W)|FD-Score:3.89|P-value:4.93E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OXR1(YPL196W)|FD-Score:-3.22|P-value:6.44E-4||SGD DESC:Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes Gene:PEX5(YDR244W)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Peroxisomal membrane signal receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins, required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions Gene:PFK2(YMR205C)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Beta subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes Gene:POL4(YCR014C)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta Gene:RAD33(YML011C)|FD-Score:3.8|P-value:7.11E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REI1(YBR267W)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network Gene:REV1(YOR346W)|FD-Score:5.36|P-value:4.27E-8||SGD DESC:Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:RGA2(YDR379W)|FD-Score:6.11|P-value:5.07E-10||SGD DESC:GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p Gene:RPL7A(YGL076C)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Ribosomal 60S subunit protein L7A; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication Gene:RPL9B(YNL067W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RPN4(YDL020C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTT101(YJL047C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p Gene:SCP160(YJL080C)|FD-Score:-3.11|P-value:9.50E-4||SGD DESC:Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins Gene:SFT2(YBL102W)|FD-Score:-3.34|P-value:4.23E-4||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SHP1(YBL058W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:UBX (ubiquitin regulatory X) domain-containing protein that regulates Glc7p phosphatase activity and interacts with Cdc48p; interacts with ubiquitylated proteins in vivo and is required for degradation of a ubiquitylated model substrate Gene:SIS2(YKR072C)|FD-Score:-3.63|P-value:1.43E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SLX1(YBR228W)|FD-Score:-5.34|P-value:4.73E-8||SGD DESC:Subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p Gene:SPS4(YOR313C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage Gene:SWC5(YBR231C)|FD-Score:-4.76|P-value:9.81E-7||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TEL1(YBL088C)|FD-Score:3.6|P-value:1.60E-4||SGD DESC:Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585) Gene:VAC7(YNL054W)|FD-Score:-3.48|P-value:2.50E-4||SGD DESC:Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock Gene:VAM3(YOR106W)|FD-Score:-3.23|P-value:6.20E-4||SGD DESC:Syntaxin-like vacuolar t-SNARE that functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region Gene:VMA13(YPR036W)|FD-Score:-3.14|P-value:8.49E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VPS1(YKR001C)|FD-Score:-3.87|P-value:5.53E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:VPS27(YNR006W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:VPS35(YJL154C)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p Gene:YAF9(YNL107W)|FD-Score:-3.22|P-value:6.30E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCR101C(YCR101C_p)|FD-Score:5.55|P-value:1.42E-8||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YER135C(YER135C_d)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR056C(YFR056C_d)|FD-Score:3.74|P-value:9.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W Gene:YIL102C(YIL102C_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function Gene:YJR018W(YJR018W_d)|FD-Score:-6.41|P-value:7.50E-11||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR257W(YLR257W_p)|FD-Score:4.29|P-value:8.82E-6||SGD DESC:Protein of unknown function; protein abundance increases in response to DNA replication stress Gene:YLR312C(YLR312C_p)|FD-Score:-4.6|P-value:2.15E-6||SGD DESC:Putative protein of unknown function Gene:YML002W(YML002W_p)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Putative protein of unknown function; expression induced by heat and by calcium shortage Gene:YNL146C-A(YNL146C-A_p)|FD-Score:-5.57|P-value:1.31E-8||SGD DESC:Putative protein of unknown function Gene:YPR108W-A(YPR108W-A_p)|FD-Score:-3.28|P-value:5.10E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:ZRC1(YMR243C)|FD-Score:3.9|P-value:4.89E-5||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YML092C5.611.02E-80.59PRE8Alpha 2 subunit of the 20S proteasome
YOR232W5.022.62E-70.25MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YHR024C4.779.29E-70.22MAS2Larger subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YGR116W4.552.66E-60.23SPT6Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region
YBR136W4.337.56E-60.20MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YLR409C4.131.82E-50.14UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YJR068W3.983.39E-50.21RFC2Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
YER147C3.787.90E-50.03SCC4Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
YJR093C3.758.93E-50.22FIP1Subunit of cleavage polyadenylation factor (CPF), interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
YHR148W3.532.07E-40.12IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YLR075W3.413.29E-40.17RPL10Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects
YPR133C3.246.04E-40.06SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YMR208W3.177.52E-40.02ERG12Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YPL153C3.167.95E-40.05RAD53Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
YDR413C_d3.119.45E-40.12YDR413C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the essential uncharacterized ORF YDR412W that is implicated in rRNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YHR209W7.716.50E-15CRG1S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
YDR379W6.115.07E-10RGA2GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p
YCR101C_p5.551.42E-8YCR101C_pPutative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
YBL003C5.531.61E-8HTA2Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
YOR346W5.364.27E-8REV1Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YJR144W4.943.89E-7MGM101Protein with a role in mitochondrial DNA recombinational repair;also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage
YPL262W4.337.57E-6FUM1Fumarase, converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
YLR257W_p4.298.82E-6YLR257W_pProtein of unknown function; protein abundance increases in response to DNA replication stress
YNR056C4.201.34E-5BIO5Putative transmembrane protein involved in the biotin biosynthesis pathway; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
YJL047C4.131.78E-5RTT101Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
YNL063W4.121.93E-5MTQ1S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
YER135C_d4.052.54E-5YER135C_dDubious open reading frame unlikely to encode a protein; YER135C is not an essential gene
YMR243C3.904.89E-5ZRC1Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
YHR073W3.894.93E-5OSH3Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
YGR254W3.865.74E-5ENO1Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_2279
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0961.33E-13SGTC_2489cantharidin 73.4 μMICCB bioactive library25450.0877193cell wall signaling
0.0808.97E-10SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.0877193cell wall signaling
0.0562.11E-5SGTC_2113455-0679 143.5 μMChemDiv (Drug-like library)7486490.262295
0.0552.63E-5SGTC_1130099-0221 2.5 μMChemDiv (Drug-like library)892190.0779221
0.0543.17E-5SGTC_2571chrysanthemic acid 100.0 μMMicrosource (Natural product library)27430.0666667
0.0526.08E-5SGTC_9891501-0895 10.3 μMChemDiv (Drug-like library)28301120.123288
0.0517.73E-5SGTC_7021488-0190 94.0 μMChemDiv (Drug-like library)9493570.0821918amide catabolism
0.0517.98E-5SGTC_7930488-0545 106.0 μMChemDiv (Drug-like library)2925480.0769231
0.0519.73E-5SGTC_15074075-3230 155.0 μMChemDiv (Drug-like library)6597710.15493
0.0501.13E-4SGTC_1540guanosine 70.6 μMTimTec (Pure natural product library)68020.0625
0.0501.40E-4SGTC_30369090181 49.5 μMChembridge (Drug-like library)252365860.179104
0.0491.47E-4SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.0327869
0.0491.56E-4SGTC_23769072922 81.3 μMChembridge (Fragment library)170879680.112676
0.0464.49E-4SGTC_20185133068 143.0 μMChembridge (Fragment library)7853620.0724638
0.0455.04E-4SGTC_1568digitonin 820.0 nMTimTec (Pure natural product library)27350100.0241935sphingolipid biosynthesis & PDR1

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_2284794507539.21 μM0.5094341245402Chembridge (Fragment library)262.35062.96414mitochondrial processes
SGTC_22827944268200 μM0.482970414Chembridge (Fragment library)242.2451262.51315RPP1 & pyrimidine depletion
SGTC_22406690706185.95 μM0.45614805876Chembridge (Fragment library)266.294562.55213
SGTC_22697935389174.42 μM0.38461597605Chembridge (Fragment library)228.312783.13912tubulin folding & SWR complex
SGTC_22917917025104.35 μM0.3442622964612Chembridge (Fragment library)280.321143.03813iron homeostasis
SGTC_23869075165157.42 μM0.29824616452277Chembridge (Fragment library)202.20931.90113
SGTC_10603448-735082 μM0.290909570178ChemDiv (Drug-like library)237.253342.91812Golgi
SGTC_1611st00185699.4 μM0.285714647478TimTec (Natural product derivative library)201.178182.07104
SGTC_9473447-0010211 μM0.266667748350ChemDiv (Drug-like library)238.24142.59813
SGTC_2113455-0679143.5 μM0.262295748649ChemDiv (Drug-like library)220.221282.87314