caffeine

1,3,7-trimethylpurine-2,6-dione

Caffeine is a phosphodiesterase inhibitor.

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PubChem MeSH terms: Central Nervous System Stimulants;Phosphodiesterase Inhibitors;Purinergic P1 Receptor Antagonists



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_228
Screen concentration 993.1 μM
Source Miscellaneous
PubChem CID 2519
SMILES CN1C=NC2=C1C(=O)N(C(=O)N2C)C
Standardized SMILES CN1C(=O)N(C)c2ncn(C)c2C1=O
Molecular weight 194.1906
ALogP -0.1
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.58
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2519
Download HIP data (tab-delimited text)  (excel)
Gene:DYS1(YHR068W)|FD-Score:-4.99|P-value:2.96E-7|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:HRT1(YOL133W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MCM4(YPR019W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.05||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:NAT2(YGR147C)|FD-Score:3.93|P-value:4.26E-5|Clearance:0.08||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PGI1(YBR196C)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.73||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RRP4(YHR069C)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.5||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:YDR187C(YDR187C_d)|FD-Score:5.47|P-value:2.26E-8|Clearance:0.99||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR190C(YGR190C_d)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:DYS1(YHR068W)|FD-Score:-4.99|P-value:2.96E-7|Clearance:0||SGD DESC:Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Gene:HRT1(YOL133W)|FD-Score:-3.27|P-value:5.41E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MCM4(YPR019W)|FD-Score:3.98|P-value:3.47E-5|Clearance:0.05||SGD DESC:Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p Gene:NAT2(YGR147C)|FD-Score:3.93|P-value:4.26E-5|Clearance:0.08||SGD DESC:Protein with an apparent role in acetylation of N-terminal methionine residues Gene:PGI1(YBR196C)|FD-Score:3.85|P-value:5.94E-5|Clearance:0.73||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RRP4(YHR069C)|FD-Score:4.48|P-value:3.75E-6|Clearance:0.5||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:YDR187C(YDR187C_d)|FD-Score:5.47|P-value:2.26E-8|Clearance:0.99||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR190C(YGR190C_d)|FD-Score:3.12|P-value:8.97E-4|Clearance:0.25||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2519
Download HOP data (tab-delimited text)  (excel)
Gene:ACE2(YLR131C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACF2(YLR144C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AHA1(YDR214W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ERJ5(YFR041C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:GEP4(YHR100C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GTR1(YML121W)|FD-Score:11|P-value:2.41E-28||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:ICS3(YJL077C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IMA1(YGR287C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:ITC1(YGL133W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LIP5(YOR196C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MCM21(YDR318W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEH1(YKR007W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MTC1(YJL123C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:PMT7(YDR307W_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RAD17(YOR368W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCE1(YMR274C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCK2(YLR248W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RMD9(YGL107C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPA34(YJL148W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:SED1(YDR077W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SLM4(YBR077C)|FD-Score:8.59|P-value:4.43E-18||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SNT309(YPR101W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSM4(YIL030C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:TCB1(YOR086C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TCO89(YPL180W)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TIF2(YJL138C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TRM11(YOL124C)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:VAB2(YEL005C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBR032W(YBR032W_d)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR336W(YDR336W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL012W(YFL012W_p)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL010W(YGL010W_p)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL089C(YNL089C_d)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YOR314W(YOR314W_d)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL257W(YPL257W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPS3(YLR121C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRR1(YOR162C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate Gene:ACE2(YLR131C)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACF2(YLR144C)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress Gene:AHA1(YDR214W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress Gene:ERJ5(YFR041C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:Type I membrane protein with a J domain is required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response Gene:GEP4(YHR100C)|FD-Score:3.73|P-value:9.62E-5||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GTR1(YML121W)|FD-Score:11|P-value:2.41E-28||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:GTR2(YGR163W)|FD-Score:6.34|P-value:1.15E-10||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:ICS3(YJL077C)|FD-Score:4.97|P-value:3.43E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:IMA1(YGR287C)|FD-Score:-3.23|P-value:6.12E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:ITC1(YGL133W)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:LIP5(YOR196C)|FD-Score:3.41|P-value:3.26E-4||SGD DESC:Protein involved in biosynthesis of the coenzyme lipoic acid, has similarity to E. coli lipoic acid synthase Gene:MCM21(YDR318W)|FD-Score:3.24|P-value:6.05E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MEH1(YKR007W)|FD-Score:4.86|P-value:5.84E-7||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MTC1(YJL123C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1 Gene:PMT7(YDR307W_p)|FD-Score:3.19|P-value:7.11E-4||SGD DESC:Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation Gene:RAD17(YOR368W)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Checkpoint protein, involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins Gene:RCE1(YMR274C)|FD-Score:-3.14|P-value:8.40E-4||SGD DESC:Type II CAAX prenyl protease involved in the proteolysis and maturation of Ras and the a-factor mating pheromone Gene:RCK2(YLR248W)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RMD9(YGL107C)|FD-Score:3.23|P-value:6.17E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPA34(YJL148W)|FD-Score:-3.19|P-value:7.22E-4||SGD DESC:RNA polymerase I subunit A34.5 Gene:SED1(YDR077W)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites Gene:SLM4(YBR077C)|FD-Score:8.59|P-value:4.43E-18||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SNT309(YPR101W)|FD-Score:-3.52|P-value:2.18E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p Gene:SSM4(YIL030C)|FD-Score:4.55|P-value:2.74E-6||SGD DESC:Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation Gene:TCB1(YOR086C)|FD-Score:3.49|P-value:2.39E-4||SGD DESC:Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact Gene:TCO89(YPL180W)|FD-Score:7.33|P-value:1.18E-13||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TIF2(YJL138C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TRM11(YOL124C)|FD-Score:-3.74|P-value:9.31E-5||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:VAB2(YEL005C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:YBR032W(YBR032W_d)|FD-Score:3.25|P-value:5.83E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YDR003W-A(YDR003W-A_p)|FD-Score:3.98|P-value:3.49E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YDR336W(YDR336W_p)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Putative protein of unknown function; sumoylated under stress conditions in a genome wide study; YDR336W is not an essential gene Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.83|P-value:6.47E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YFL012W(YFL012W_p)|FD-Score:3.79|P-value:7.56E-5||SGD DESC:Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin Gene:YGL010W(YGL010W_p)|FD-Score:3.24|P-value:6.04E-4||SGD DESC:Putative protein of unknown function; YGL010W is not an essential gene Gene:YIL025C(YIL025C_d)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YNL089C(YNL089C_d)|FD-Score:3.86|P-value:5.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins Gene:YOR314W(YOR314W_d)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPK1(YKL126W)|FD-Score:3.1|P-value:9.81E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL257W(YPL257W_p)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene Gene:YPS3(YLR121C)|FD-Score:3.46|P-value:2.73E-4||SGD DESC:Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:YRR1(YOR162C)|FD-Score:-3.19|P-value:7.20E-4||SGD DESC:Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication Gene:ZIP1(YDR285W)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR187C_d5.472.26E-80.99YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YHR069C4.483.75E-60.50RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YPR019W3.983.47E-50.05MCM4Essential helicase component of heterohexameric MCM2-7 complexes which bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
YGR147C3.934.26E-50.08NAT2Protein with an apparent role in acetylation of N-terminal methionine residues
YBR196C3.855.94E-50.73PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YGR190C_d3.128.97E-40.25YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YDR361C2.880.002010.10BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDL152W_d2.770.002799.08E-5YDL152W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
YOR319W2.770.002790.00HSH49U2-snRNP associated splicing factor with similarity to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
YOL097C2.770.002810.14WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YOL034W2.630.004310.23SMC5Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
YIL118W2.400.008210.05RHO3Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
YPL093W2.350.009470.15NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YPL044C_d2.190.014100.01YPL044C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
YNL114C_d2.190.014400.01YNL114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YML121W11.002.41E-28GTR1Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
YBR077C8.594.43E-18SLM4Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
YPL180W7.331.18E-13TCO89Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
YGR163W6.341.15E-10GTR2Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
YJL077C4.973.43E-7ICS3Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2
YKR007W4.865.84E-7MEH1Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
YIL030C4.552.74E-6SSM4Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
YDR285W4.072.37E-5ZIP1Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; potential Cdc28p substrate
YDR003W-A_p3.983.49E-5YDR003W-A_pPutative protein of unknown function; identified by expression profiling and mass spectrometry
YNL089C_d3.865.61E-5YNL089C_dDubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins
YDR077W3.865.65E-5SED1Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites
YFL012W_p3.797.56E-5YFL012W_pPutative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin
YHR100C3.739.62E-5GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YLR131C3.551.95E-4ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YDR214W3.502.35E-4AHA1Co-chaperone that binds to Hsp82p and activates its ATPase activity; similar to Hch1p; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress

GO enrichment analysis for SGTC_228
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.2128.79E-61SGTC_21775811945 192.0 μMChembridge (Fragment library)53434750.132075
0.1803.20E-44SGTC_560957-0365 83.2 μMChemDiv (Drug-like library)67523600.0526316Golgi
0.1778.71E-43SGTC_1844rapamycin 4.0 nMMiscellaneous52846160.057377
0.1738.47E-41SGTC_1877wortmannin 25.0 μMICCB bioactive library3121450.0595238
0.1716.03E-40SGTC_3111315-0136 58.0 μMChemDiv (Drug-like library)67592070.0434783
0.1498.30E-31SGTC_1734st037168 69.4 μMTimTec (Natural product derivative library)7089070.510638
0.1369.63E-26SGTC_20265148700 152.0 μMChembridge (Fragment library)798380.333333
0.1331.18E-24SGTC_166k072-0058 166.4 μMChemDiv (Drug-like library)58462290.046875DNA intercalators
0.1292.98E-23SGTC_16014'-hydroxyflavanone 55.5 μMTimTec (Pure natural product library)1655060.0727273RPP1 & pyrimidine depletion
0.1187.35E-20SGTC_21275253358 187.9 μMChembridge (Fragment library)206120.0666667
0.1159.64E-19SGTC_2679rapamycin 1.0 nMMicrosource (Natural product library)52846160.057377
0.1119.76E-18SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0943396TSC3-RPN4
0.1102.91E-17SGTC_170960-0276 49.8 μMChemDiv (Drug-like library)68211140.0487805
0.1062.86E-16SGTC_5741315-0397 40.0 μMChemDiv (Drug-like library)67942770.0506329
0.1063.74E-16SGTC_780336-0123 156.8 μMChemDiv (Drug-like library)67658960.0434783

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_1675st01848524.5 μM0.5853663323024TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_23387999974200 μM0.55315925Chembridge (Fragment library)234.254460.96303
SGTC_1676st01848923.4 μM0.5365853696235TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_1734st03716869.4 μM0.510638708907TimTec (Natural product derivative library)288.2770231.68904
SGTC_1743st03760646.38 μM0.488889590142TimTec (Natural product derivative library)326.392882.88403
SGTC_1736st03677451 μM0.3958331272247TimTec (Natural product derivative library)392.47414.17904
SGTC_23759072683200 μM0.39130416798029Chembridge (Fragment library)261.27980.54703mitochondrial processes
SGTC_2348902254333.42 μM0.3777783162678Chembridge (Fragment library)275.306380.8303mitochondrial processes
SGTC_1740st03828613.22 μM0.369565716786TimTec (Natural product derivative library)266.319461.29213
SGTC_1748st03745553.6 μM0.352941580690TimTec (Natural product derivative library)339.176683.02403