7945142

N-(3-carbamoylthiophen-2-yl)pyridine-3-carboxamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2285
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2970608
SMILES C1=CC(=CN=C1)C(=O)NC2=C(C=CS2)C(=O)N
Standardized SMILES NC(=O)c1ccsc1NC(=O)c2cccnc2
Molecular weight 247.2731
ALogP 0.25
H-bond donor count 2
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.99
% growth inhibition (Hom. pool) 1.03


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2970608
Download HIP data (tab-delimited text)  (excel)
Gene:ACT1(YFL039C)|FD-Score:-3.38|P-value:3.59E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BBP1(YPL255W)|FD-Score:-3.15|P-value:8.07E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:CBF5(YLR175W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.06||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT8(YJL008C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.05||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG8(YMR220W)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FCP1(YMR277W)|FD-Score:5.66|P-value:7.41E-9|Clearance:1.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HEM2(YGL040C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.16||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HRR25(YPL204W)|FD-Score:-4.67|P-value:1.54E-6|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HYP2(YEL034W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IPL1(YPL209C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.17||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:MSL5(YLR116W)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NAR1(YNL240C)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.15||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NCP1(YHR042W)|FD-Score:7.17|P-value:3.85E-13|Clearance:0.99||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NUP49(YGL172W)|FD-Score:3.77|P-value:8.09E-5|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRE10(YOR362C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PSF1(YDR013W)|FD-Score:10.1|P-value:3.11E-24|Clearance:2.61||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RAT1(YOR048C)|FD-Score:-4.84|P-value:6.43E-7|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLP24(YLR009W)|FD-Score:4.57|P-value:2.47E-6|Clearance:0.48||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RNA1(YMR235C)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.02||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPL15A(YLR029C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL3(YOR063W)|FD-Score:4.63|P-value:1.83E-6|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:SPC110(YDR356W)|FD-Score:-4.69|P-value:1.39E-6|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TAF10(YDR167W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TBF1(YPL128C)|FD-Score:-4.28|P-value:9.20E-6|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:THS1(YIL078W)|FD-Score:7.47|P-value:3.90E-14|Clearance:0.31||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TRS120(YDR407C)|FD-Score:6.18|P-value:3.19E-10|Clearance:0.52||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC9(YDL064W)|FD-Score:-3.41|P-value:3.24E-4|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YJR141W(YJR141W_p)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.03||SGD DESC:Essential protein of unknown function Gene:YOR218C(YOR218C_d)|FD-Score:3.94|P-value:4.01E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:ACT1(YFL039C)|FD-Score:-3.38|P-value:3.59E-4|Clearance:0||SGD DESC:Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functions Gene:BBP1(YPL255W)|FD-Score:-3.15|P-value:8.07E-4|Clearance:0||SGD DESC:Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periphery; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p Gene:CBF5(YLR175W)|FD-Score:3.24|P-value:5.98E-4|Clearance:0.06||SGD DESC:Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita Gene:CCT8(YJL008C)|FD-Score:3.18|P-value:7.49E-4|Clearance:0.05||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.98E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ERG8(YMR220W)|FD-Score:-3.43|P-value:3.05E-4|Clearance:0||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:FCP1(YMR277W)|FD-Score:5.66|P-value:7.41E-9|Clearance:1.03||SGD DESC:Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p) Gene:HEM2(YGL040C)|FD-Score:3.62|P-value:1.47E-4|Clearance:0.16||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HRR25(YPL204W)|FD-Score:-4.67|P-value:1.54E-6|Clearance:0||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HYP2(YEL034W)|FD-Score:-4.22|P-value:1.21E-5|Clearance:0||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IPL1(YPL209C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.17||SGD DESC:Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest Gene:MSL5(YLR116W)|FD-Score:-3.26|P-value:5.50E-4|Clearance:0||SGD DESC:Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA Gene:NAR1(YNL240C)|FD-Score:4.09|P-value:2.16E-5|Clearance:0.15||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:NCP1(YHR042W)|FD-Score:7.17|P-value:3.85E-13|Clearance:0.99||SGD DESC:NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p Gene:NUP49(YGL172W)|FD-Score:3.77|P-value:8.09E-5|Clearance:0.07||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p) Gene:PRE10(YOR362C)|FD-Score:-3.57|P-value:1.80E-4|Clearance:0||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PSF1(YDR013W)|FD-Score:10.1|P-value:3.11E-24|Clearance:2.61||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RAT1(YOR048C)|FD-Score:-4.84|P-value:6.43E-7|Clearance:0||SGD DESC:Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snRNA processing as well as poly (A+) dependent and independent mRNA transcription termination Gene:RLP24(YLR009W)|FD-Score:4.57|P-value:2.47E-6|Clearance:0.48||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RNA1(YMR235C)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.02||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:RPL15A(YLR029C)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.08||SGD DESC:Ribosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication Gene:RPL3(YOR063W)|FD-Score:4.63|P-value:1.83E-6|Clearance:0.06||SGD DESC:Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus Gene:SPC110(YDR356W)|FD-Score:-4.69|P-value:1.39E-6|Clearance:0||SGD DESC:Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner Gene:TAF10(YDR167W)|FD-Score:3.12|P-value:8.99E-4|Clearance:0.03||SGD DESC:Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification Gene:TBF1(YPL128C)|FD-Score:-4.28|P-value:9.20E-6|Clearance:0||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:THS1(YIL078W)|FD-Score:7.47|P-value:3.90E-14|Clearance:0.31||SGD DESC:Threonyl-tRNA synthetase, essential cytoplasmic protein Gene:TRS120(YDR407C)|FD-Score:6.18|P-value:3.19E-10|Clearance:0.52||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic Gene:UBC9(YDL064W)|FD-Score:-3.41|P-value:3.24E-4|Clearance:0||SGD DESC:SUMO-conjugating enzyme involved in the Smt3p conjugation pathway; nuclear protein required for S- and M-phase cyclin degradation and mitotic control; involved in proteolysis mediated by the anaphase-promoting complex cyclosome (APCC) Gene:YJR141W(YJR141W_p)|FD-Score:3.27|P-value:5.45E-4|Clearance:0.03||SGD DESC:Essential protein of unknown function Gene:YOR218C(YOR218C_d)|FD-Score:3.94|P-value:4.01E-5|Clearance:0.17||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2970608
Download HOP data (tab-delimited text)  (excel)
Gene:AEP2(YMR282C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM17(YHL021C)|FD-Score:4.3|P-value:8.43E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APE4(YHR113W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APL1(YJR005W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BSP1(YPR171W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CHS6(YJL099W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CNN1(YFR046C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COX13(YGL191W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:CST26(YBR042C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DPB3(YBR278W)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EAF6(YJR082C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM2(YBR065C)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM32(YER176W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERG5(YMR015C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERI1(YPL096C-A)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FCY21(YER060W)|FD-Score:-6.07|P-value:6.43E-10||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FSF1(YOR271C_p)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GPM3(YOL056W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HXK1(YFR053C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC14(YOR135C_d)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:LRP1(YHR081W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MDS3(YGL197W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP6(YHR015W)|FD-Score:4.7|P-value:1.27E-6||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRPL32(YCR003W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSY1(YPL097W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NOP13(YNL175C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NOP16(YER002W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:OAR1(YKL055C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPT2(YPR194C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAU4(YLR461W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PET123(YOR158W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO3(YBR092C)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PNP1(YLR209C)|FD-Score:6.49|P-value:4.22E-11||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:POC4(YPL144W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PSO2(YMR137C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUS7(YOR243C)|FD-Score:4.3|P-value:8.52E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QDR2(YIL121W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPL7B(YPL198W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:SEG1(YMR086W)|FD-Score:5.92|P-value:1.61E-9||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SEM1(YDR363W-A)|FD-Score:-5.19|P-value:1.04E-7||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGO1(YOR073W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SOL1(YNR034W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SOV1(YMR066W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPG1(YGR236C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPT4(YGR063C)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSH1(YBR283C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SYH1(YPL105C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TEF4(YKL081W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:THI4(YGR144W)|FD-Score:4.81|P-value:7.59E-7||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TIF2(YJL138C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMA20(YER007C-A)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TMA22(YJR014W)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO2(YGR138C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UME1(YPL139C)|FD-Score:-4.67|P-value:1.49E-6||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPS27(YNR006W)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP1802(YGR241C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDR186C(YDR186C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR246W-A(YDR246W-A_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER053C-A(YER053C-A_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YFL015C(YFL015C_d)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGR250C(YGR250C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL213W(YJL213W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL071W(YKL071W_p)|FD-Score:9.89|P-value:2.31E-23||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR149C(YLR149C_p)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR315W(YMR315W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOL029C(YOL029C_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOL097W-A(YOL097W-A_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR385W(YOR385W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR097W(YPR097W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YRB30(YGL164C)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ZEO1(YOL109W)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:AEP2(YMR282C)|FD-Score:3.99|P-value:3.25E-5||SGD DESC:Mitochondrial protein, likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader Gene:AIM17(YHL021C)|FD-Score:4.3|P-value:8.43E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALG5(YPL227C)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:UDP-glucose:dolichyl-phosphate glucosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum Gene:APE4(YHR113W)|FD-Score:3.18|P-value:7.26E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:APL1(YJR005W)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex Gene:BSP1(YPR171W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Adapter that links synaptojanins Inp52p and Inp53p to the cortical actin cytoskeleton Gene:CHS6(YJL099W)|FD-Score:3.58|P-value:1.70E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CMC4(YMR194C-B)|FD-Score:4.02|P-value:2.95E-5||SGD DESC:Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs Gene:CNN1(YFR046C)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T Gene:COX13(YGL191W)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes Gene:CST26(YBR042C)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication Gene:CUE1(YMR264W)|FD-Score:3.31|P-value:4.59E-4||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DPB3(YBR278W)|FD-Score:-3.73|P-value:9.66E-5||SGD DESC:Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication Gene:EAF6(YJR082C)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:ECM2(YBR065C)|FD-Score:5.78|P-value:3.70E-9||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM32(YER176W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:DNA dependent ATPase/DNA helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes Gene:ERG5(YMR015C)|FD-Score:3.93|P-value:4.18E-5||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:ERI1(YPL096C-A)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Endoplasmic reticulum membrane protein that binds to and inhibits GTP-bound Ras2p at the ER; component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein Gene:FCY21(YER060W)|FD-Score:-6.07|P-value:6.43E-10||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:FSF1(YOR271C_p)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Putative protein, predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis Gene:GPM3(YOL056W)|FD-Score:-3.86|P-value:5.65E-5||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:HXK1(YFR053C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication Gene:IES5(YER092W)|FD-Score:3.43|P-value:3.07E-4||SGD DESC:Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions Gene:IRC14(YOR135C_d)|FD-Score:5.09|P-value:1.82E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:LRP1(YHR081W)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination Gene:MDS3(YGL197W)|FD-Score:3.44|P-value:2.86E-4||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MIP6(YHR015W)|FD-Score:4.7|P-value:1.27E-6||SGD DESC:Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication Gene:MMT2(YPL224C)|FD-Score:3.27|P-value:5.36E-4||SGD DESC:Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p Gene:MRPL32(YCR003W)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MSY1(YPL097W)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Mitochondrial tyrosyl-tRNA synthetase Gene:NOP13(YNL175C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NOP16(YER002W)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis Gene:OAR1(YKL055C)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPT2(YPR194C)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Oligopeptide transporter; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles Gene:PAU4(YLR461W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme Gene:PET123(YOR158W)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PHO3(YBR092C)|FD-Score:-3.76|P-value:8.46E-5||SGD DESC:Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin Gene:PNP1(YLR209C)|FD-Score:6.49|P-value:4.22E-11||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:POC4(YPL144W)|FD-Score:-3.3|P-value:4.76E-4||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PSO2(YMR137C)|FD-Score:3.81|P-value:6.87E-5||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:PUS7(YOR243C)|FD-Score:4.3|P-value:8.52E-6||SGD DESC:Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria Gene:QDR2(YIL121W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper Gene:RPL7B(YPL198W)|FD-Score:-3.18|P-value:7.39E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RPL8A(YHL033C)|FD-Score:4.89|P-value:5.08E-7||SGD DESC:Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication Gene:RPS10A(YOR293W)|FD-Score:-3.62|P-value:1.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication Gene:SEG1(YMR086W)|FD-Score:5.92|P-value:1.61E-9||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SEM1(YDR363W-A)|FD-Score:-5.19|P-value:1.04E-7||SGD DESC:Component of lid subcomplex of the 26S proteasome regulatory subunit; involved in mRNA export mediated by the TREX-2 complex (Sac3p-Thp1p); ortholog of human DSS1; protein abundance increases in response to DNA replication stress Gene:SET5(YHR207C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SGO1(YOR073W)|FD-Score:-3.1|P-value:9.67E-4||SGD DESC:Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability Gene:SOL1(YNR034W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SOV1(YMR066W)|FD-Score:-4.08|P-value:2.26E-5||SGD DESC:Mitochondrial protein of unknown function Gene:SPG1(YGR236C)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SPT4(YGR063C)|FD-Score:-3.16|P-value:7.98E-4||SGD DESC:Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions Gene:SSH1(YBR283C)|FD-Score:-3.29|P-value:5.02E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SYH1(YPL105C)|FD-Score:3.76|P-value:8.40E-5||SGD DESC:Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication Gene:TEF4(YKL081W)|FD-Score:3.16|P-value:7.81E-4||SGD DESC:Gamma subunit of translational elongation factor eEF1B, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex Gene:THI4(YGR144W)|FD-Score:4.81|P-value:7.59E-7||SGD DESC:Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents Gene:TIF2(YJL138C)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication Gene:TMA20(YER007C-A)|FD-Score:-4.22|P-value:1.20E-5||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:TMA22(YJR014W)|FD-Score:-4.03|P-value:2.74E-5||SGD DESC:Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress Gene:TOM70(YNL121C)|FD-Score:4.37|P-value:6.26E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPO2(YGR138C)|FD-Score:3.22|P-value:6.34E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication Gene:UBA4(YHR111W)|FD-Score:4.35|P-value:6.84E-6||SGD DESC:Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p Gene:UME1(YPL139C)|FD-Score:-4.67|P-value:1.49E-6||SGD DESC:Negative regulator of meiosis, required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p, Wtm2p Gene:VPS27(YNR006W)|FD-Score:3.2|P-value:6.93E-4||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP1802(YGR241C)|FD-Score:4.14|P-value:1.70E-5||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication Gene:YDR186C(YDR186C)|FD-Score:4.63|P-value:1.81E-6||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR246W-A(YDR246W-A_p)|FD-Score:4.16|P-value:1.62E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YER053C-A(YER053C-A_p)|FD-Score:4.23|P-value:1.15E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress Gene:YER067C-A(YER067C-A_d)|FD-Score:-3.95|P-value:3.88E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.16|P-value:7.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YFL015C(YFL015C_d)|FD-Score:-3.72|P-value:9.79E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene Gene:YGR250C(YGR250C)|FD-Score:3.2|P-value:6.82E-4||SGD DESC:Putative RNA binding protein; localizes to stress granules induced by glucose deprivation; interacts with Rbg1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:YJL213W(YJL213W)|FD-Score:3.38|P-value:3.59E-4||SGD DESC:Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p Gene:YKL071W(YKL071W_p)|FD-Score:9.89|P-value:2.31E-23||SGD DESC:Putative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YLR149C(YLR149C_p)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YMR031W-A(YMR031W-A_d)|FD-Score:-3.5|P-value:2.35E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C Gene:YMR315W(YMR315W)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress Gene:YOL029C(YOL029C_p)|FD-Score:3.31|P-value:4.65E-4||SGD DESC:Putative protein of unknown function; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens Gene:YOL097W-A(YOL097W-A_p)|FD-Score:4.12|P-value:1.90E-5||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR385W(YOR385W_p)|FD-Score:3.47|P-value:2.61E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPL034W(YPL034W_p)|FD-Score:-3.92|P-value:4.51E-5||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR097W(YPR097W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YRB30(YGL164C)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes Gene:ZEO1(YOL109W)|FD-Score:-4.65|P-value:1.70E-6||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR013W10.103.11E-242.61PSF1Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
YIL078W7.473.90E-140.31THS1Threonyl-tRNA synthetase, essential cytoplasmic protein
YHR042W7.173.85E-130.99NCP1NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
YDR407C6.183.19E-100.52TRS120One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YMR277W5.667.41E-91.03FCP1Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
YOR063W4.631.83E-60.06RPL3Ribosomal 60S subunit protein L3; homologous to mammalian ribosomal protein L3 and bacterial L3; involved in the replication and maintenance of killer double stranded RNA virus
YLR009W4.572.47E-60.48RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YNL240C4.092.16E-50.15NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YOR218C_d3.944.01E-50.17YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YGL172W3.778.09E-50.07NUP49FG-nucleoporin component of central core of the nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)
YLR029C3.701.06E-40.08RPL15ARibosomal 60S subunit protein L15A; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15A has a paralog, RPL15B, that arose from the whole genome duplication
YGL040C3.621.47E-40.17HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YMR235C3.462.74E-40.02RNA1GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
YPL209C3.442.95E-40.17IPL1Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest
YJR141W_p3.275.45E-40.03YJR141W_pEssential protein of unknown function

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL071W_p9.892.31E-23YKL071W_pPutative protein of unknown function; expression induced in cells treated with the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YLR209C6.494.22E-11PNP1Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
YMR086W5.921.61E-9SEG1Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes
YBR065C5.783.70E-9ECM2Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
YOR135C_d5.091.82E-7IRC14_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
YHL033C4.895.08E-7RPL8ARibosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication
YGR144W4.817.59E-7THI4Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
YLR461W4.741.06E-6PAU4Member of the seripauperin multigene family encoded mainly in subtelomeric regions; active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
YHR015W4.701.27E-6MIP6Putative RNA-binding protein; interacts with Mex67p, which is a component of the nuclear pore involved in nuclear mRNA export; MIP6 has a paralog, PES4, that arose from the whole genome duplication
YDR186C4.631.81E-6YDR186CPutative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YNL121C4.376.26E-6TOM70Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication
YHR111W4.356.84E-6UBA4Protein that activates Urm1p before its conjugation to proteins (urmylation); also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
YHL021C4.308.43E-6AIM17Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
YOR243C4.308.52E-6PUS7Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); conserved in archaea, vertebrates, and some bacteria
YIL121W4.241.14E-5QDR2Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper

GO enrichment analysis for SGTC_2285
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0621.86E-6SGTC_9322857-0803 154.0 μMChemDiv (Drug-like library)6748980.169492
0.0518.34E-5SGTC_33389144300 39.6 μMChembridge (Drug-like library)49012740.164384Golgi
0.0519.85E-5SGTC_30249090631 49.5 μMChembridge (Drug-like library)252363830.112676
0.0511.01E-4SGTC_14364092-1007 8.3 μMChemDiv (Drug-like library)28811210.056338
0.0491.79E-4SGTC_13641653-0028 37.6 μMChemDiv (Drug-like library)57186520.091954
0.0472.84E-4SGTC_2410aloe-emodin-8-o-glycoside 46.3 μMTimTec (Pure natural product library)140774150.060241mitochondrial processes
0.0472.88E-4SGTC_30099080819 71.4 μMChembridge (Drug-like library)170272160.164179
0.0472.97E-4SGTC_28759039966 71.4 μMChembridge (Drug-like library)64636910.0869565
0.0473.20E-4SGTC_14434112-3303 14.0 μMChemDiv (Drug-like library)7044330.136364
0.0455.38E-4SGTC_1693xanthone 101.9 μMTimTec (Natural product derivative library)70200.1DNA damage response
0.0439.31E-4SGTC_28809042983 58.4 μMChembridge (Drug-like library)64647140.126761fatty acid desaturase (OLE1)
0.0430.00106SGTC_396dithiazanine 1.2 μMMiscellaneous105780.0617284
0.0420.00129SGTC_110438-0307 1.3 μMChemDiv (Drug-like library)40114760.0392157
0.0410.00165SGTC_1553gossypol 38.6 μMTimTec (Pure natural product library)35030.0447761
0.0400.00197SGTC_33059127027 53.8 μMChembridge (Drug-like library)419052950.0657895

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2274794709446.08 μM0.72971028Chembridge (Fragment library)246.2851.40323
SGTC_22647948757110.76 μM0.5681822971411Chembridge (Fragment library)252.312721.35624
SGTC_22637948595193.29 μM0.5319152971371Chembridge (Fragment library)236.247120.79824
SGTC_3022908799349.47 μM0.50909117173644Chembridge (Drug-like library)351.422182.40224
SGTC_3025908789549.47 μM0.48275917173641Chembridge (Drug-like library)365.448762.88924PDR1
SGTC_3034909042149.47 μM0.46428617173761Chembridge (Drug-like library)304.364142.381560S ribosome export
SGTC_3032908942749.47 μM0.44067817173707Chembridge (Drug-like library)318.390722.7291560S ribosome export
SGTC_3151909982149.47 μM0.40983617174189Chembridge (Drug-like library)329.416662.03624
SGTC_2967908953849.85 μM0.4728216Chembridge (Drug-like library)300.663633.0715
SGTC_2800788793581.82 μM0.3928572961002Chembridge (Drug-like library)340.826624.24613RPP1 & pyrimidine depletion