7945887

methyl 2-(propanoylamino)thiophene-3-carboxylate

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2287
Screen concentration 168.8 μM
Source Chembridge (Fragment library)
PubChem CID 1245853
SMILES CCC(=O)NC1=C(C=CS1)C(=O)OC
Standardized SMILES CCC(=O)Nc1sccc1C(=O)OC
Molecular weight 213.2535
ALogP 1.26
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 16.35
% growth inhibition (Hom. pool) 6.17


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1245853
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:HIP1(YGR191W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.01||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:MDN1(YLR106C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NHP2(YDL208W)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.39||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:POL1(YNL102W)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PSF1(YDR013W)|FD-Score:-7.49|P-value:3.43E-14|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIB3(YDR487C)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:TFC8(YPL007C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.16||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TRS130(YMR218C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:YGR190C(YGR190C_d)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YLR140W(YLR140W_d)|FD-Score:3.73|P-value:9.49E-5|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:AFG2(YLR397C)|FD-Score:-3.11|P-value:9.35E-4|Clearance:0||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:HIP1(YGR191W)|FD-Score:3.25|P-value:5.83E-4|Clearance:0.01||SGD DESC:High-affinity histidine permease, also involved in the transport of manganese ions Gene:MDN1(YLR106C)|FD-Score:-3.39|P-value:3.52E-4|Clearance:0||SGD DESC:Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus Gene:NHP2(YDL208W)|FD-Score:4.12|P-value:1.91E-5|Clearance:0.39||SGD DESC:Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing Gene:POL1(YNL102W)|FD-Score:-3.84|P-value:6.10E-5|Clearance:0||SGD DESC:Catalytic subunit of the DNA polymerase I alpha-primase complex, required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis Gene:PSF1(YDR013W)|FD-Score:-7.49|P-value:3.43E-14|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RIB3(YDR487C)|FD-Score:-3.43|P-value:2.97E-4|Clearance:0||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:TFC8(YPL007C)|FD-Score:3.24|P-value:6.04E-4|Clearance:0.16||SGD DESC:One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90 Gene:TRS130(YMR218C)|FD-Score:-3.29|P-value:5.07E-4|Clearance:0||SGD DESC:One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy Gene:YGR190C(YGR190C_d)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.26||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:YLR140W(YLR140W_d)|FD-Score:3.73|P-value:9.49E-5|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1245853
Download HOP data (tab-delimited text)  (excel)
Gene:ACN9(YDR511W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AIM46(YHR199C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ATG1(YGL180W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:AVT2(YEL064C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BMH2(YDR099W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNA6(YFR047C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD28(YLR062C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIS3(YJL158C)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:COX16(YJL003W)|FD-Score:-3.89|P-value:5.00E-5||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:ERV15(YBR210W)|FD-Score:-3.77|P-value:8.18E-5||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FMP33(YJL161W_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDH1(YOR375C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GPG1(YGL121C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:GTS1(YGL181W)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GTT1(YIR038C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GYP5(YPL249C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HAL1(YPR005C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:INO1(YJL153C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:LSM6(YDR378C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRPL33(YMR286W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OAR1(YKL055C)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PFK27(YOL136C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PHO88(YBR106W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMP2(YEL017C-A)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PMT5(YDL093W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PPT2(YPL148C)|FD-Score:6.82|P-value:4.53E-12||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PTC7(YHR076W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RPS22B(YLR367W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTT102(YGR275W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SCW11(YGL028C)|FD-Score:5.12|P-value:1.50E-7||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SFC1(YJR095W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SPP1(YPL138C)|FD-Score:-6.17|P-value:3.42E-10||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:STM1(YLR150W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TMA20(YER007C-A)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR015C(YCR015C_p)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function Gene:YDR222W(YDR222W_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR401W(YDR401W_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEA4(YEL004W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YER038W-A(YER038W-A_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER091C-A(YER091C-A_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR226C(YGR226C_d)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YHL037C(YHL037C_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL027C(YJL027C_p)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function Gene:YJL215C(YJL215C_d)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.53|P-value:2.88E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR124W(YLR124W_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR003C(YPR003C_p)|FD-Score:-3.73|P-value:9.40E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZEO1(YOL109W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria Gene:ACN9(YDR511W)|FD-Score:-3.24|P-value:6.07E-4||SGD DESC:Protein of the mitochondrial intermembrane space, required for acetate utilization and gluconeogenesis; has orthologs in higher eukaryotes Gene:AIM46(YHR199C)|FD-Score:3.33|P-value:4.37E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ATG1(YGL180W)|FD-Score:3.33|P-value:4.33E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:AVT2(YEL064C)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BMH2(YDR099W)|FD-Score:3.34|P-value:4.26E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNA6(YFR047C)|FD-Score:3.31|P-value:4.68E-4||SGD DESC:Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p Gene:BUD28(YLR062C_d)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CIS3(YJL158C)|FD-Score:4.01|P-value:2.97E-5||SGD DESC:Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family Gene:COX16(YJL003W)|FD-Score:-3.89|P-value:5.00E-5||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:ERV15(YBR210W)|FD-Score:-3.77|P-value:8.18E-5||SGD DESC:Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication Gene:FMP33(YJL161W_p)|FD-Score:3.25|P-value:5.86E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GDH1(YOR375C)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GPG1(YGL121C)|FD-Score:-3.19|P-value:7.15E-4||SGD DESC:Proposed gamma subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing Gene:GTS1(YGL181W)|FD-Score:4.87|P-value:5.65E-7||SGD DESC:Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations Gene:GTT1(YIR038C)|FD-Score:4.12|P-value:1.88E-5||SGD DESC:ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p Gene:GYP5(YPL249C)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:HAL1(YPR005C)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p Gene:INO1(YJL153C)|FD-Score:3.22|P-value:6.40E-4||SGD DESC:Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element Gene:LSM6(YDR378C)|FD-Score:-3.23|P-value:6.17E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA Gene:MRPL33(YMR286W)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:OAR1(YKL055C)|FD-Score:-4.62|P-value:1.88E-6||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:PFK27(YOL136C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:6-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A Gene:PHO88(YBR106W)|FD-Score:3.26|P-value:5.60E-4||SGD DESC:Probable membrane protein, involved in phosphate transport; pho88 pho86 double null mutant exhibits enhanced synthesis of repressible acid phosphatase at high inorganic phosphate concentrations Gene:PMP2(YEL017C-A)|FD-Score:3.23|P-value:6.28E-4||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PMT5(YDL093W)|FD-Score:3.34|P-value:4.20E-4||SGD DESC:Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals Gene:PPT2(YPL148C)|FD-Score:6.82|P-value:4.53E-12||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:PTC7(YHR076W)|FD-Score:-3.26|P-value:5.54E-4||SGD DESC:Type 2C protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope Gene:RPS22B(YLR367W)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication Gene:RSM19(YNR037C)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S19 ribosomal protein Gene:RTT102(YGR275W)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress Gene:SCS7(YMR272C)|FD-Score:-3.51|P-value:2.24E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SCW11(YGL028C)|FD-Score:5.12|P-value:1.50E-7||SGD DESC:Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p Gene:SFC1(YJR095W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SPP1(YPL138C)|FD-Score:-6.17|P-value:3.42E-10||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:STM1(YLR150W)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery Gene:TMA20(YER007C-A)|FD-Score:-3.36|P-value:3.87E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress Gene:YAL067W-A(YAL067W-A_p)|FD-Score:-3.5|P-value:2.29E-4||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YCR015C(YCR015C_p)|FD-Score:-3.8|P-value:7.28E-5||SGD DESC:Putative protein of unknown function; YCR015C is not an essential gene Gene:YCR100C(YCR100C_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function Gene:YDR222W(YDR222W_p)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YDR401W(YDR401W_d)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEA4(YEL004W)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YER038W-A(YER038W-A_d)|FD-Score:4.01|P-value:3.08E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria Gene:YER091C-A(YER091C-A_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR226C(YGR226C_d)|FD-Score:4.51|P-value:3.27E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W Gene:YHL037C(YHL037C_d)|FD-Score:-3.17|P-value:7.56E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIP4(YGL198W)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport Gene:YJL027C(YJL027C_p)|FD-Score:-3.27|P-value:5.47E-4||SGD DESC:Putative protein of unknown function Gene:YJL215C(YJL215C_d)|FD-Score:-3.38|P-value:3.63E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YKL068W-A(YKL068W-A_p)|FD-Score:4.53|P-value:2.88E-6||SGD DESC:Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i> Gene:YLR124W(YLR124W_d)|FD-Score:-3.38|P-value:3.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR003C(YPR003C_p)|FD-Score:-3.73|P-value:9.40E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR197C(YPR197C_d)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ZEO1(YOL109W)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL208W4.121.91E-50.39NHP2Nuclear protein related to mammalian high mobility group (HMG) proteins, essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
YLR140W_d3.739.49E-50.22YLR140W_dDubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
YGR190C_d3.512.26E-40.26YGR190C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
YGR191W3.255.83E-40.01HIP1High-affinity histidine permease, also involved in the transport of manganese ions
YPL007C3.246.04E-40.16TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90
YOR353C3.080.001050.22SOG2Key component of the RAM signaling network, required for proper cell morphogenesis and cell separation after mitosis
YLL003W2.860.002140.01SFI1Centrin (Cdc31p)-binding protein required for spindle pole body (SPB) duplication, localizes to the half-bridge of the SPB, required for progression through G(2)-M transition, has similarity to Xenopus laevis XCAP-C
YDR361C2.850.002210.09BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YBR152W2.760.002910.03SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YDR396W_d2.730.003150.03YDR396W_dDubious open reading frame unlikely to encode a functional protein; extensively overlaps essential NCB2 gene encoding the beta subunit of the NC2 dimeric histone-fold complex
YMR239C2.700.003450.02RNT1Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
YER082C2.680.003630.02UTP7Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
YDL031W2.670.003830.04DBP10Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
YKL042W2.630.004330.06SPC42Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
YLR379W_d2.560.005180.06YLR379W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL148C6.824.53E-12PPT2Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
YGL028C5.121.50E-7SCW11Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
YGL181W4.875.65E-7GTS1Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
YKL068W-A_p4.532.88E-6YKL068W-A_pPutative protein of unknown function; identified by homology to Ashbya gossypii
YGR226C_d4.513.27E-6YGR226C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
YIR038C4.121.88E-5GTT1ER associated glutathione S-transferase capable of homodimerization; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
YJL158C4.012.97E-5CIS3Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
YER038W-A_d4.013.08E-5YER038W-A_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 99% of ORF overlaps the verified gene HVG1; protein product detected in mitochondria
YOL136C3.993.37E-5PFK276-phosphofructo-2-kinase, catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
YOL109W3.701.08E-4ZEO1Peripheral membrane protein of the plasma membrane that interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
YJR095W3.691.13E-4SFC1Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization
YLR367W3.601.57E-4RPS22BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
YLR062C_d3.561.87E-4BUD28_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YDL093W3.344.20E-4PMT5Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals
YDR099W3.344.26E-4BMH214-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress

GO enrichment analysis for SGTC_2287
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1172.13E-19SGTC_22807785178 200.0 μMChembridge (Fragment library)29543280.129032
0.1094.15E-17SGTC_22817943571 200.0 μMChembridge (Fragment library)12440240.189655
0.0831.87E-10SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.106061
0.0791.10E-9SGTC_25422-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 100.0 μMMicrosource (Natural product library)50247450.0166667
0.0741.28E-8SGTC_24045161160 200.0 μMMiscellaneous28315430.101449
0.0715.77E-8SGTC_22847945075 39.2 μMChembridge (Fragment library)12454020.183333mitochondrial processes
0.0681.90E-7SGTC_22226885208 172.1 μMChembridge (Fragment library)6095140.0862069
0.0595.87E-6SGTC_1648st011736 69.8 μMTimTec (Natural product derivative library)28555140.0588235PDR1
0.0543.15E-5SGTC_6470140-0236 81.0 μMChemDiv (Drug-like library)9908370.1
0.0543.62E-5SGTC_1628st004252 77.7 μMTimTec (Natural product derivative library)6929680.112903
0.0534.19E-5SGTC_24275545055 42.8 μMMiscellaneous53728580.0819672
0.0534.59E-5SGTC_31459085516 49.5 μMChembridge (Drug-like library)252361950.147541fatty acid desaturase (OLE1)
0.0535.03E-5SGTC_1722st034304 3.7 μMTimTec (Natural product derivative library)40361480.0727273mitochondrial processes
0.0535.04E-5SGTC_289k017-0060 526.0 μMChemDiv (Drug-like library)55129090.0769231
0.0525.48E-5SGTC_31029121669 49.5 μMChembridge (Drug-like library)419050720.09375

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22199098279200 μM0.377778902349Chembridge (Fragment library)213.253540.73714
SGTC_2274794709446.08 μM0.3695652971028Chembridge (Fragment library)246.2851.40323
SGTC_22647948757110.76 μM0.3617022971411Chembridge (Fragment library)252.312721.35624
SGTC_3206911435049.47 μM0.3617087243Chembridge (Drug-like library)283.321783.53513
SGTC_3193911193149.47 μM0.35294117087244Chembridge (Drug-like library)348.191263.79713
SGTC_3194911190349.47 μM0.35294117087251Chembridge (Drug-like library)303.740263.71313
SGTC_22637948595193.29 μM0.342971371Chembridge (Fragment library)236.247120.79824
SGTC_2796794963445.45 μM0.3333332971588Chembridge (Drug-like library)366.433523.11515
SGTC_22857945142200 μM0.3269232970608Chembridge (Fragment library)247.273060.25224
SGTC_9861493-048384 μM0.3240079ChemDiv (Drug-like library)256.256681.31914