benomyl

methyl N-[1-(butylcarbamoyl)benzimidazol-2-yl]carbamate


Established Yeast Drug Target : TUB1 TUB2

Benomyl is a fungal microtubule inhibitor often used in molecular biology to study the cell cycle in yeast.

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 

PubChem MeSH terms: Anthelmintics;Fungicides, Industrial;Tubulin Modulators



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_229
Screen concentration 22.9 μM
Source Miscellaneous
PubChem CID 28780
SMILES CCCCNC(=O)N1C2=CC=CC=C2N=C1NC(=O)OC
Standardized SMILES CCCCNC(=O)n1c(NC(=O)OC)nc2ccccc12
Molecular weight 290.3177
ALogP 2.97
H-bond donor count 2
H-bond acceptor count 4
Response signature tubulin folding & SWR complex

Pool Growth Kinetics
% growth inhibition (Het. pool) 4.04
% growth inhibition (Hom. pool) -1.59


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 28780
Download HIP data (tab-delimited text)  (excel)
Gene:ALG2(YGL065C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.02||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CCT2(YIL142W)|FD-Score:10.1|P-value:3.88E-24|Clearance:1.64||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:9.92|P-value:1.78E-23|Clearance:1.64||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:8.19|P-value:1.32E-16|Clearance:0.68||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:5.52|P-value:1.73E-8|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:5.97|P-value:1.19E-9|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:7.5|P-value:3.08E-14|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC2(YDL209C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.27||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG10(YPL028W)|FD-Score:3.22|P-value:6.46E-4|Clearance:0.13||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MAS1(YLR163C)|FD-Score:7.31|P-value:1.38E-13|Clearance:0.95||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MPS3(YJL019W)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.03||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOB1(YOR056C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.01||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NUP159(YIL115C)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.15||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRP28(YDR243C)|FD-Score:5.11|P-value:1.63E-7|Clearance:1.39||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:QNS1(YHR074W)|FD-Score:5.85|P-value:2.51E-9|Clearance:0.33||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPM2(YML091C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:SMD3(YLR147C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:TCP1(YDR212W)|FD-Score:9.82|P-value:4.41E-23|Clearance:1.64||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TUB2(YFL037W)|FD-Score:6.35|P-value:1.07E-10|Clearance:0.38||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YDR187C(YDR187C_d)|FD-Score:11.4|P-value:3.40E-30|Clearance:1.64||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR190C(YGR190C_d)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W Gene:ALG2(YGL065C)|FD-Score:3.65|P-value:1.29E-4|Clearance:0.02||SGD DESC:Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol Gene:CCT2(YIL142W)|FD-Score:10.1|P-value:3.88E-24|Clearance:1.64||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:9.92|P-value:1.78E-23|Clearance:1.64||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT5(YJR064W)|FD-Score:8.19|P-value:1.32E-16|Clearance:0.68||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT6(YDR188W)|FD-Score:5.52|P-value:1.73E-8|Clearance:0.41||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif Gene:CCT7(YJL111W)|FD-Score:5.97|P-value:1.19E-9|Clearance:0.12||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:7.5|P-value:3.08E-14|Clearance:0.2||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CWC2(YDL209C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.27||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:ERG10(YPL028W)|FD-Score:3.22|P-value:6.46E-4|Clearance:0.13||SGD DESC:Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase), cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis Gene:MAS1(YLR163C)|FD-Score:7.31|P-value:1.38E-13|Clearance:0.95||SGD DESC:Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins Gene:MPS3(YJL019W)|FD-Score:3.25|P-value:5.85E-4|Clearance:0.03||SGD DESC:Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p Gene:NOB1(YOR056C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.01||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NUP159(YIL115C)|FD-Score:-3.53|P-value:2.08E-4|Clearance:0||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p) Gene:PIS1(YPR113W)|FD-Score:3.09|P-value:9.99E-4|Clearance:0.15||SGD DESC:Phosphatidylinositol synthase, required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins Gene:PRP28(YDR243C)|FD-Score:5.11|P-value:1.63E-7|Clearance:1.39||SGD DESC:RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site Gene:QNS1(YHR074W)|FD-Score:5.85|P-value:2.51E-9|Clearance:0.33||SGD DESC:Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide Gene:RPM2(YML091C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus Gene:SMD3(YLR147C)|FD-Score:3.72|P-value:1.01E-4|Clearance:0.06||SGD DESC:Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3 Gene:TCP1(YDR212W)|FD-Score:9.82|P-value:4.41E-23|Clearance:1.64||SGD DESC:Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein Gene:TUB2(YFL037W)|FD-Score:6.35|P-value:1.07E-10|Clearance:0.38||SGD DESC:Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria Gene:YDR187C(YDR187C_d)|FD-Score:11.4|P-value:3.40E-30|Clearance:1.64||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YGR190C(YGR190C_d)|FD-Score:3.36|P-value:3.95E-4|Clearance:0.11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 28780
Download HOP data (tab-delimited text)  (excel)
Gene:ADH5(YBR145W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALF1(YNL148C)|FD-Score:6.08|P-value:5.95E-10||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP6(YLR085C)|FD-Score:16|P-value:1.33E-57||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BIK1(YCL029C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CBC2(YPL178W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CIN1(YOR349W)|FD-Score:18.6|P-value:3.73E-77||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:12.6|P-value:7.92E-37||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CIN4(YMR138W)|FD-Score:12|P-value:3.04E-33||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN2(YPL256C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMK2(YOL016C)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DIP5(YPL265W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:ERV25(YML012W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:ETR1(YBR026C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP30(YPL103C)|FD-Score:4.76|P-value:9.67E-7||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUS2(YMR232W)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GBP2(YCL011C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GIS3(YLR094C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein of unknown function Gene:HTZ1(YOL012C)|FD-Score:8.36|P-value:3.16E-17||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICS3(YJL077C)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:LAS21(YJL062W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MBA1(YBR185C)|FD-Score:6.16|P-value:3.53E-10||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCM21(YDR318W)|FD-Score:4.31|P-value:8.33E-6||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MCM22(YJR135C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MLH3(YPL164C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRS3(YJL133W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OYE3(YPL171C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAC2(YER007W)|FD-Score:46.4|P-value:0||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAM1(YDR251W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PAN2(YGL094C)|FD-Score:5.91|P-value:1.76E-9||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN3(YKL025C)|FD-Score:8.63|P-value:3.03E-18||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDC5(YLR134W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDC6(YGR087C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PPZ2(YDR436W)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:REF2(YDR195W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RFU1(YLR073C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:ROM1(YGR070W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:SAK1(YER129W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCD6(YPR129W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SEC28(YIL076W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEE1(YIL064W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIL1(YOL031C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SPC1(YJR010C-A)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SWC3(YAL011W)|FD-Score:15.1|P-value:7.57E-52||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:12.6|P-value:8.27E-37||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:8.88|P-value:3.35E-19||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TGL3(YMR313C)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TRP4(YDR354W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:20.8|P-value:3.24E-96||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS71(YML041C)|FD-Score:20.7|P-value:4.17E-95||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:10.7|P-value:5.83E-27||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAF9(YNL107W)|FD-Score:7.95|P-value:8.97E-16||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR071W(YBR071W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCR064C(YCR064C_d)|FD-Score:6.6|P-value:2.12E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR249C(YDR249C_p)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR124W(YLR124W_d)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL296W(YNL296W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:ADH5(YBR145W)|FD-Score:3.15|P-value:8.05E-4||SGD DESC:Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication Gene:AHC2(YCR082W)|FD-Score:4.08|P-value:2.25E-5||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ALF1(YNL148C)|FD-Score:6.08|P-value:5.95E-10||SGD DESC:Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance Gene:ARP6(YLR085C)|FD-Score:16|P-value:1.33E-57||SGD DESC:Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:BIK1(YCL029C)|FD-Score:5.44|P-value:2.60E-8||SGD DESC:Microtubule-associated protein, component of the interface between microtubules and kinetochore, involved in sister chromatid separation; essential in polyploid cells but not in haploid or diploid cells; ortholog of mammalian CLIP-170 Gene:CBC2(YPL178W)|FD-Score:3.34|P-value:4.24E-4||SGD DESC:Small subunit of the heterodimeric cap binding complex that also contains Sto1p, component of the spliceosomal commitment complex; interacts with Npl3p, possibly to package mRNA for export from the nucleus; contains an RNA-binding motif Gene:CIN1(YOR349W)|FD-Score:18.6|P-value:3.73E-77||SGD DESC:Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl Gene:CIN2(YPL241C)|FD-Score:12.6|P-value:7.92E-37||SGD DESC:GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2 Gene:CIN4(YMR138W)|FD-Score:12|P-value:3.04E-33||SGD DESC:GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog Gene:CLN2(YPL256C)|FD-Score:3.23|P-value:6.24E-4||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMK2(YOL016C)|FD-Score:4.35|P-value:6.77E-6||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication Gene:DIP5(YPL265W)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress Gene:ERV25(YML012W)|FD-Score:-3.1|P-value:9.53E-4||SGD DESC:Protein that forms a heterotrimeric complex with Erp1, Erp2p, and Emp24, member of the p24 family involved in endoplasmic reticulum to Golgi transport Gene:ETR1(YBR026C)|FD-Score:3.24|P-value:5.97E-4||SGD DESC:2-enoyl thioester reductase, member of the medium chain dehydrogenase/reductase family; localized to in mitochondria, where it has a probable role in fatty acid synthesis Gene:FMP30(YPL103C)|FD-Score:4.76|P-value:9.67E-7||SGD DESC:Mitochondrial inner membrane protein with a role in maintaining mitochondrial morphology and normal cardiolipin levels; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D Gene:FUS2(YMR232W)|FD-Score:3.81|P-value:6.97E-5||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GBP2(YCL011C)|FD-Score:3.2|P-value:6.76E-4||SGD DESC:Poly(A+) RNA-binding protein; involved in the export of mRNAs from the nucleus to the cytoplasm; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; GBP2 has a paralog, HRB1, that arose from the whole genome duplication Gene:GIS3(YLR094C)|FD-Score:4.26|P-value:1.03E-5||SGD DESC:Protein of unknown function Gene:HTZ1(YOL012C)|FD-Score:8.36|P-value:3.16E-17||SGD DESC:Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin Gene:ICS3(YJL077C)|FD-Score:5.08|P-value:1.87E-7||SGD DESC:Protein of unknown function; may play a role in vacuolar sorting, null mutants are hypersensitive to sortin2 Gene:LAS21(YJL062W)|FD-Score:-3.15|P-value:8.15E-4||SGD DESC:Integral plasma membrane protein involved in the synthesis of the glycosylphosphatidylinositol (GPI) core structure; mutations affect cell wall integrity Gene:MBA1(YBR185C)|FD-Score:6.16|P-value:3.53E-10||SGD DESC:Membrane-associated mitochondrial ribosome receptor; forms a complex with Mdm38p that may facilitate recruitment of mRNA-specific translational activators to ribosomes; possible role in protein export from the matrix to inner membrane Gene:MCM21(YDR318W)|FD-Score:4.31|P-value:8.33E-6||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MCM22(YJR135C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MLH3(YPL164C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Protein involved in DNA mismatch repair and crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability Gene:MRS3(YJL133W)|FD-Score:3.96|P-value:3.71E-5||SGD DESC:Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication Gene:NUP2(YLR335W)|FD-Score:3.42|P-value:3.13E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:OYE3(YPL171C)|FD-Score:3.66|P-value:1.24E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PAC2(YER007W)|FD-Score:46.4|P-value:0||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PAM1(YDR251W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype Gene:PAN2(YGL094C)|FD-Score:5.91|P-value:1.76E-9||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PAN3(YKL025C)|FD-Score:8.63|P-value:3.03E-18||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PDC5(YLR134W)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PDC6(YGR087C)|FD-Score:3.29|P-value:4.93E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation Gene:PPZ2(YDR436W)|FD-Score:4.39|P-value:5.73E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:REF2(YDR195W)|FD-Score:3.74|P-value:9.06E-5||SGD DESC:RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes Gene:RFU1(YLR073C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:ROM1(YGR070W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:SAK1(YER129W)|FD-Score:3.44|P-value:2.87E-4||SGD DESC:Upstream serine/threonine kinase for the SNF1 complex; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome Gene:SCD6(YPR129W)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress Gene:SEC28(YIL076W)|FD-Score:3.19|P-value:7.03E-4||SGD DESC:Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress Gene:SEE1(YIL064W)|FD-Score:3.53|P-value:2.08E-4||SGD DESC:Probable lysine methyltransferase involved in the dimethylation of eEF1A (Tef1p/Tef2p); sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport Gene:SIL1(YOL031C)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SPC1(YJR010C-A)|FD-Score:3.77|P-value:8.31E-5||SGD DESC:Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress Gene:SWC3(YAL011W)|FD-Score:15.1|P-value:7.57E-52||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWC5(YBR231C)|FD-Score:12.6|P-value:8.27E-37||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:SWR1(YDR334W)|FD-Score:8.88|P-value:3.35E-19||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:TGL3(YMR313C)|FD-Score:5.02|P-value:2.60E-7||SGD DESC:Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation Gene:TRP4(YDR354W)|FD-Score:3.35|P-value:4.04E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUB3(YML124C)|FD-Score:20.8|P-value:3.24E-96||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication Gene:VAC17(YCL063W)|FD-Score:-3.7|P-value:1.06E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VPS71(YML041C)|FD-Score:20.7|P-value:4.17E-95||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS72(YDR485C)|FD-Score:10.7|P-value:5.83E-27||SGD DESC:Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:VPS75(YNL246W)|FD-Score:3.83|P-value:6.40E-5||SGD DESC:NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress Gene:YAF9(YNL107W)|FD-Score:7.95|P-value:8.97E-16||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YBR071W(YBR071W)|FD-Score:3.18|P-value:7.37E-4||SGD DESC:Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking Gene:YCR064C(YCR064C_d)|FD-Score:6.6|P-value:2.12E-11||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR249C(YDR249C_p)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:Putative protein of unknown function Gene:YGL034C(YGL034C_d)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.28|P-value:5.14E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR124W(YLR124W_d)|FD-Score:3.79|P-value:7.64E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YML094C-A(YML094C-A_d)|FD-Score:3.15|P-value:8.16E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YNL296W(YNL296W_d)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog

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Top 15 HET fitness defect scores (HIP)

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Deletion Strain FD score P-value Clearance Gene Gene Description
YDR187C_d11.403.40E-301.64YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YIL142W10.103.88E-241.64CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL014W9.921.78E-231.64CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR212W9.824.41E-231.64TCP1Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
YJR064W8.191.32E-160.68CCT5Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YJL008C7.503.08E-140.20CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR163C7.311.38E-130.95MAS1Smaller subunit of the mitochondrial processing protease (MPP), essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
YFL037W6.351.07E-100.38TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YJL111W5.971.19E-90.12CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YHR074W5.852.51E-90.33QNS1Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide
YDR188W5.521.73E-80.41CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YDR243C5.111.63E-71.39PRP28RNA helicase in the DEAD-box family, involved in RNA isomerization at the 5' splice site
YLR147C3.721.01E-40.06SMD3Core Sm protein Sm D3; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D3
YGL065C3.651.29E-40.02ALG2Mannosyltransferase that catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol
YOR056C3.631.40E-40.01NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress

Top 15 HOM fitness defect scores (HOP)

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Deletion Strain FD score P-value Gene Gene Description
YER007W46.400PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YML124C20.803.24E-96TUB3Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
YML041C20.704.17E-95VPS71Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YOR349W18.603.73E-77CIN1Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
YLR085C16.001.33E-57ARP6Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YAL011W15.107.57E-52SWC3Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
YPL241C12.607.92E-37CIN2GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
YBR231C12.608.27E-37SWC5Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
YMR138W12.003.04E-33CIN4GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
YDR485C10.705.83E-27VPS72Htz1p-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
YDR334W8.883.35E-19SWR1Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
YKL025C8.633.03E-18PAN3Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
YOL012C8.363.16E-17HTZ1Histone variant H2AZ, exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin
YNL107W7.958.97E-16YAF9Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain
YCR064C_d6.602.12E-11YCR064C_dDubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31

GO enrichment analysis for SGTC_229
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.6890SGTC_21745790901 200.0 μMChembridge (Fragment library)7860200.179104tubulin folding & SWR complex
0.6860SGTC_23196033012 152.6 μMChembridge (Fragment library)8472460.166667tubulin folding & SWR complex
0.6170SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.171053tubulin folding & SWR complex
0.5950SGTC_1875nocodazole 6.0 μMMiscellaneous41220.267606tubulin folding & SWR complex
0.5740SGTC_2123807-4682 38.8 μMChemDiv (Drug-like library)31255010.1tubulin folding & SWR complex
0.5430SGTC_13261431-2094 10.8 μMChemDiv (Drug-like library)6162780.177419tubulin folding & SWR complex
0.5210SGTC_2103551-0445 56.8 μMChemDiv (Drug-like library)28651800.148148
0.5040SGTC_21255246583 200.0 μMChembridge (Fragment library)8352670.164179tubulin folding & SWR complex
0.4680SGTC_22006600604 21.9 μMChembridge (Fragment library)29047770.0895522tubulin folding & SWR complex
0.4562.68E-301SGTC_21415621638 7.7 μMChembridge (Fragment library)28562380.0909091tubulin folding & SWR complex
0.4513.14E-293SGTC_5891227-0070 34.1 μMChemDiv (Drug-like library)28413840.0972222
0.4408.37E-278SGTC_14334060-0023 7.6 μMChemDiv (Drug-like library)38406270.169014tubulin folding & SWR complex
0.4091.58E-236SGTC_3753970-0961 9.3 μMChemDiv (Drug-like library)28982220.1tubulin folding & SWR complex
0.4079.76E-235SGTC_1493970-0822 46.1 μMChemDiv (Drug-like library)31142500.1125tubulin folding & SWR complex
0.3939.82E-217SGTC_1643st009866 20.2 μMTimTec (Natural product derivative library)31562730.0833333

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_7223534-0461190 μM0.370968658262ChemDiv (Drug-like library)293.362963.9512PDR1
SGTC_3252913587149.47 μM0.3174617682838Chembridge (Drug-like library)245.27711.51122
SGTC_2785775646372.73 μM0.303032201551Chembridge (Drug-like library)308.334543.33214
SGTC_23459052315157.45 μM0.2881366469265Chembridge (Fragment library)193.24561.48922
SGTC_3288911969036.22 μM0.28787917595618Chembridge (Drug-like library)295.378843.00702
SGTC_2427554505542.75 μM0.2786895372858Miscellaneous250.298463.71113
SGTC_7924534-1957155 μM0.2727271717904ChemDiv (Drug-like library)282.337022.73412
SGTC_21996590217200 μM0.269841703109Chembridge (Fragment library)227.261822.93613mitochondrial processes
SGTC_1875nocodazole6 μM0.2676064122Miscellaneous301.320443.00825tubulin folding & SWR complex
SGTC_3017908068149.47 μM0.26470616451914Chembridge (Drug-like library)307.389543.14702