7971431

(2Z)-2-(thiophen-3-ylmethylidene)-1-azabicyclo[2.2.2]octan-3-ol

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2294
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 5741572
SMILES C1CN2CCC1C(C2=CC3=CSC=C3)O
Standardized SMILES OC1C2CCN(CC2)C1=Cc3ccsc3
Molecular weight 221.3186
ALogP 1.52
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.46
% growth inhibition (Hom. pool) 2.26


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5741572
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:3.36|P-value:3.97E-4|Clearance:0.15||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CAK1(YFL029C)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.2||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:HEM15(YOR176W)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.05||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HRP1(YOL123W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:IQG1(YPL242C)|FD-Score:-3.99|P-value:3.34E-5|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:RIO1(YOR119C)|FD-Score:4.67|P-value:1.47E-6|Clearance:0.67||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPT4(YOR259C)|FD-Score:4.95|P-value:3.71E-7|Clearance:0.67||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRB1(YMR131C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.67||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC5(YDR166C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.08||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:TIF6(YPR016C)|FD-Score:4.86|P-value:6.01E-7|Clearance:0.67||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIP20(YGL145W)|FD-Score:4.85|P-value:6.02E-7|Clearance:0.67||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p Gene:APC5(YOR249C)|FD-Score:3.36|P-value:3.97E-4|Clearance:0.15||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CAK1(YFL029C)|FD-Score:3.15|P-value:8.12E-4|Clearance:0.2||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:HEM15(YOR176W)|FD-Score:3.2|P-value:6.86E-4|Clearance:0.05||SGD DESC:Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway Gene:HRP1(YOL123W)|FD-Score:-4|P-value:3.17E-5|Clearance:0||SGD DESC:Subunit of cleavage factor I, a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences Gene:IQG1(YPL242C)|FD-Score:-3.99|P-value:3.34E-5|Clearance:0||SGD DESC:Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MCM2(YBL023C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:RIO1(YOR119C)|FD-Score:4.67|P-value:1.47E-6|Clearance:0.67||SGD DESC:Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA Gene:RPT4(YOR259C)|FD-Score:4.95|P-value:3.71E-7|Clearance:0.67||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRB1(YMR131C)|FD-Score:4.11|P-value:2.00E-5|Clearance:0.67||SGD DESC:Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p Gene:SEC5(YDR166C)|FD-Score:3.43|P-value:2.98E-4|Clearance:0.08||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:TIF6(YPR016C)|FD-Score:4.86|P-value:6.01E-7|Clearance:0.67||SGD DESC:Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits Gene:TIP20(YGL145W)|FD-Score:4.85|P-value:6.02E-7|Clearance:0.67||SGD DESC:Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5741572
Download HOP data (tab-delimited text)  (excel)
Gene:AIM13(YFR011C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APJ1(YNL077W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ASG1(YIL130W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATP12(YJL180C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BFA1(YJR053W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CDC26(YFR036W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLC1(YGR167W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CRN1(YLR429W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CSG2(YBR036C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIT1(YDR403W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM10(YEL030W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP52(YER004W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAL1(YBR020W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GAL11(YOL051W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDB1(YPR184W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIR2(YDR152W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLR1(YPL091W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HOP2(YGL033W)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ISU1(YPL135W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KIN2(YLR096W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MAM33(YIL070C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MMS1(YPR164W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MOT2(YER068W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPS28(YDR337W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN2(YMR037C)|FD-Score:-7.3|P-value:1.41E-13||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTL1(YGR023W)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:MUP3(YHL036W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NUM1(YDR150W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OAR1(YKL055C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPI10(YOL032W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:POC4(YPL144W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PRY2(YKR013W)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD27(YKL113C)|FD-Score:7.79|P-value:3.35E-15||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RFU1(YLR073C)|FD-Score:-3.96|P-value:3.67E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL31A(YDL075W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSC1(YGR056W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SKI7(YOR076C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLZ1(YNL196C_p)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SPG5(YMR191W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRL1(YOR247W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDH2(YJR009C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TOM5(YPR133W-A)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TSL1(YML100W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:URA10(YMR271C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VID22(YLR373C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS1(YKR001C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YAR028W(YAR028W_p)|FD-Score:-5.01|P-value:2.68E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR284W(YBR284W_p)|FD-Score:5.29|P-value:6.18E-8||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR043C(YCR043C_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YEL1(YBL060W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER134C(YER134C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER135C(YER135C_d)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR020W(YFR020W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR273C(YGR273C_p)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJL022W(YJL022W_d)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKR033C(YKR033C_d)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL058W(YLL058W_p)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function Gene:YMR114C(YMR114C)|FD-Score:-6.24|P-value:2.20E-10||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YNR021W(YNR021W_p)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOR111W(YOR111W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Putative protein of unknown function Gene:YPL034W(YPL034W_p)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR089W(YPR089W_p)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p Gene:AIM13(YFR011C)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Mitochondrial protein peripheral to the inner membrane; component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:APJ1(YNL077W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Chaperone with a role in SUMO-mediated protein degradation; member of the DnaJ-like family; conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress Gene:ASG1(YIL130W)|FD-Score:3.13|P-value:8.79E-4||SGD DESC:Zinc cluster protein proposed to function as a transcriptional regulator involved in the stress response; null mutants have a respiratory deficiency, calcofluor white sensitivity and slightly increased cycloheximide resistance Gene:ATP12(YJL180C)|FD-Score:3.11|P-value:9.41E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:BFA1(YJR053W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CDC26(YFR036W)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CLC1(YGR167W)|FD-Score:-3.09|P-value:9.85E-4||SGD DESC:Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W Gene:CRN1(YLR429W)|FD-Score:-3.5|P-value:2.32E-4||SGD DESC:Coronin, cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly Gene:CSG2(YBR036C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress Gene:DIA1(YMR316W)|FD-Score:-3.14|P-value:8.58E-4||SGD DESC:Protein of unknown function, involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:DIT1(YDR403W)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:ECM10(YEL030W)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication Gene:FMP21(YBR269C_p)|FD-Score:3.28|P-value:5.23E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP52(YER004W)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:GAL1(YBR020W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication Gene:GAL11(YOL051W)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GDB1(YPR184W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress Gene:GIR2(YDR152W)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Highly-acidic cytoplasmic RWD domain-containing protein of unknown function; forms a complex with Rbg2p; interacts with Rbg1p and Gcn1p; associates with translating ribosomes; putative intrinsically unstructured protein Gene:GLR1(YPL091W)|FD-Score:-3.13|P-value:8.64E-4||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GSF2(YML048W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:ER localized integral membrane protein that may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression Gene:HOP2(YGL033W)|FD-Score:5.68|P-value:6.62E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:ISU1(YPL135W)|FD-Score:3.21|P-value:6.58E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KIN2(YLR096W)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p Gene:MAM33(YIL070C)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:Acidic protein of the mitochondrial matrix involved in oxidative phosphorylation; related to the human complement receptor gC1q-R Gene:MMS1(YPR164W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MOT2(YER068W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications Gene:MRPS28(YDR337W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSN2(YMR037C)|FD-Score:-7.3|P-value:1.41E-13||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MTC7(YEL033W_p)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant Gene:MTL1(YGR023W)|FD-Score:-4.4|P-value:5.51E-6||SGD DESC:Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p Gene:MUP3(YHL036W)|FD-Score:-3.12|P-value:9.02E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NUM1(YDR150W)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OAR1(YKL055C)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p Gene:OPI10(YOL032W)|FD-Score:-3.67|P-value:1.22E-4||SGD DESC:Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress Gene:POC4(YPL144W)|FD-Score:-4.01|P-value:3.00E-5||SGD DESC:Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions Gene:PRY2(YKR013W)|FD-Score:4.35|P-value:6.95E-6||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:PTH1(YHR189W)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:One of two (see also PTH2) mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium Gene:RAD27(YKL113C)|FD-Score:7.79|P-value:3.35E-15||SGD DESC:5' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family Gene:RFU1(YLR073C)|FD-Score:-3.96|P-value:3.67E-5||SGD DESC:Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes Gene:RPL31A(YDL075W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication Gene:RPL7B(YPL198W)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication Gene:RRD1(YIL153W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:RSC1(YGR056W)|FD-Score:-3.75|P-value:8.69E-5||SGD DESC:Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook Gene:SKI7(YOR076C)|FD-Score:-3.13|P-value:8.70E-4||SGD DESC:Coupling protein that mediates interactions between the Ski complex and the cytoplasmic exosome during 3'-5' RNA degradation; eRF3-like domain targets nonstop mRNA for degradation; null mutants have superkiller phenotype Gene:SLZ1(YNL196C_p)|FD-Score:-3.15|P-value:8.06E-4||SGD DESC:Sporulation-specific protein with a leucine zipper motif Gene:SPG5(YMR191W)|FD-Score:3.11|P-value:9.31E-4||SGD DESC:Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRL1(YOR247W)|FD-Score:3.6|P-value:1.59E-4||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:SVL3(YPL032C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:TDH2(YJR009C)|FD-Score:-3.16|P-value:8.00E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:TOM5(YPR133W-A)|FD-Score:3.43|P-value:3.03E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore Gene:TSL1(YML100W)|FD-Score:4.2|P-value:1.32E-5||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TUS1(YLR425W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) that modulate Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate Gene:URA10(YMR271C)|FD-Score:-3.31|P-value:4.59E-4||SGD DESC:Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication Gene:VID22(YLR373C)|FD-Score:3.23|P-value:6.18E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:VPS1(YKR001C)|FD-Score:3.9|P-value:4.75E-5||SGD DESC:Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis Gene:YAR028W(YAR028W_p)|FD-Score:-5.01|P-value:2.68E-7||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YBR284W(YBR284W_p)|FD-Score:5.29|P-value:6.18E-8||SGD DESC:Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication Gene:YCR043C(YCR043C_p)|FD-Score:4.03|P-value:2.75E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YCR087W(YCR087W_d)|FD-Score:5.17|P-value:1.17E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene Gene:YEL1(YBL060W)|FD-Score:-3.13|P-value:8.71E-4||SGD DESC:Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip Gene:YER087C-A(YER087C-A_d)|FD-Score:-3.4|P-value:3.41E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER134C(YER134C)|FD-Score:-3.26|P-value:5.61E-4||SGD DESC:Magnesium-dependent acid phosphatase, member of the haloacid dehalogenase superfamily; non-essential gene Gene:YER135C(YER135C_d)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein; YER135C is not an essential gene Gene:YFR020W(YFR020W_p)|FD-Score:3.18|P-value:7.46E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGR273C(YGR273C_p)|FD-Score:-3.88|P-value:5.19E-5||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHC3(YJL059W)|FD-Score:-3.22|P-value:6.52E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJL022W(YJL022W_d)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130 Gene:YKR033C(YKR033C_d)|FD-Score:-3.11|P-value:9.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL058W(YLL058W_p)|FD-Score:3.8|P-value:7.14E-5||SGD DESC:Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene Gene:YLR406C-A(YLR406C-A_p)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Putative protein of unknown function Gene:YMR114C(YMR114C)|FD-Score:-6.24|P-value:2.20E-10||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene Gene:YNR021W(YNR021W_p)|FD-Score:-3.87|P-value:5.36E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene Gene:YNR061C(YNR061C_p)|FD-Score:3.15|P-value:8.19E-4||SGD DESC:Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress Gene:YOR111W(YOR111W_p)|FD-Score:4.68|P-value:1.44E-6||SGD DESC:Putative protein of unknown function Gene:YPL034W(YPL034W_p)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Putative protein of unknown function; YPL034W is not essential gene Gene:YPR089W(YPR089W_p)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR259C4.953.71E-70.67RPT4One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization
YPR016C4.866.01E-70.67TIF6Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
YGL145W4.856.02E-70.67TIP20Peripheral membrane protein required for fusion of COPI vesicles with the ER; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
YOR119C4.671.47E-60.67RIO1Essential serine kinase involved in cell cycle progression and processing of the 20S pre-rRNA into mature 18S rRNA
YMR131C4.112.00E-50.67RRB1Essential nuclear protein involved in early steps of ribosome biogenesis; physically interacts with the ribosomal protein Rpl3p
YDR166C3.432.98E-40.08SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YOR249C3.363.97E-40.15APC5Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
YBL023C3.206.81E-40.00MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YOR176W3.206.86E-40.05HEM15Ferrochelatase, a mitochondrial inner membrane protein, catalyzes the insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway
YFL029C3.158.12E-40.20CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YDR167W2.950.001570.05TAF10Subunit (145 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
YJL109C2.910.001820.03UTP10Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
YGL247W2.870.002030.03BRR6Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
YOL010W2.840.002240.08RCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
YML025C2.770.002830.00YML6Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL113C7.793.35E-15RAD275' to 3' exonuclease, 5' flap endonuclease, required for Okazaki fragment processing and maturation as well as for long-patch base-excision repair; member of the S. pombe RAD2/FEN1 family
YGL033W5.686.62E-9HOP2Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
YPR164W5.571.24E-8MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YBR284W_p5.296.18E-8YBR284W_pPutative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
YCR087W_d5.171.17E-7YCR087W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
YOR111W_p4.681.44E-6YOR111W_pPutative protein of unknown function
YPL034W_p4.395.65E-6YPL034W_pPutative protein of unknown function; YPL034W is not essential gene
YKR013W4.356.95E-6PRY2Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication
YEL033W_p4.241.13E-5MTC7_pPredicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
YKL055C4.201.31E-5OAR1Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase, may comprise a type II mitochondrial fatty acid synthase along with Mct1p
YML100W4.201.32E-5TSL1Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
YCR043C_p4.032.75E-5YCR043C_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
YKR001C3.904.75E-5VPS1Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
YLR096W3.865.74E-5KIN2Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p
YLL058W_p3.807.14E-5YLL058W_pPutative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene

GO enrichment analysis for SGTC_2294
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0756.30E-9SGTC_7874152-0075 138.0 μMChemDiv (Drug-like library)95665390.0769231
0.0648.18E-7SGTC_15074075-3230 155.0 μMChemDiv (Drug-like library)6597710.0724638
0.0622.15E-6SGTC_21055350118 200.0 μMChembridge (Fragment library)21333130.0983607
0.0595.04E-6SGTC_1997st073024 93.8 μMTimTec (Natural product derivative library)6862680.0491803
0.0571.23E-5SGTC_22937624732 200.0 μMChembridge (Fragment library)8944530.0454545
0.0571.24E-5SGTC_990109-0120 622.8 μMChemDiv (Drug-like library)7594140.0491803
0.0561.72E-5SGTC_348k064-0012 10.7 μMChemDiv (Drug-like library)50663230.04761960S ribosome export
0.0552.19E-5SGTC_30359091303 49.5 μMChembridge (Drug-like library)252370020.0952381
0.0542.87E-5SGTC_31699102970 49.5 μMChembridge (Drug-like library)170278390.0615385RPP1 & pyrimidine depletion
0.0543.63E-5SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.0735294RNA pol III & RNase P/MRP
0.0534.62E-5SGTC_14294049-0205 9.5 μMChemDiv (Drug-like library)227744780.047619
0.0519.51E-5SGTC_2751anetholtrithion 17.2 μMMiscellaneous21940.0535714
0.0501.41E-4SGTC_1555citronellol 128.0 μMTimTec (Pure natural product library)88420.0377358
0.0491.47E-4SGTC_20165121758 140.0 μMChembridge (Fragment library)27952030.08redox potentiating
0.0491.49E-4SGTC_14344073-0095 163.0 μMChemDiv (Drug-like library)241337450.0506329

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23379009472200 μM0.5454555742383Chembridge (Fragment library)229.317462.34812
SGTC_2165572719732.14 μM0.31251361712Chembridge (Fragment library)211.26081.60414
SGTC_21906049624200 μM0.2452832265554Chembridge (Fragment library)229.27441.86313
SGTC_1712st03228853.4 μM0.2181822883011TimTec (Natural product derivative library)374.348284.05314calcium & mitochondrial duress
SGTC_21896047310154.43 μM0.210526711535Chembridge (Fragment library)243.300982.08903RPP1 & pyrimidine depletion
SGTC_3133474-00405.03 μM0.1607141043375ChemDiv (Drug-like library)356.3335.00603NEO1-PIK1
SGTC_11313454-312832.3 μM0.15873710982ChemDiv (Drug-like library)267.328983.1104
SGTC_2795778001054.55 μM0.15873953673Chembridge (Drug-like library)312.3812434.15113
SGTC_11473809-104512.3 μM0.156863325925ChemDiv (Drug-like library)293.402723.42322
SGTC_12810884-003111.8 μM0.1525421548255ChemDiv (Drug-like library)366.498045.21202fatty acid desaturase (OLE1)