7971656

9-(furan-2-yl)-8,9,10,11-tetrahydrobenzo[b][1,4]benzodiazepin-7-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2296
Screen concentration 126.5 μM
Source Chembridge (Fragment library)
PubChem CID 666570
SMILES C1C(CC(=O)C2=C1NC3=CC=CC=C3N=C2)C4=CC=CO4
Standardized SMILES O=C1CC(CC2=Nc3ccccc3NC=C12)c4occc4
Molecular weight 278.3053
ALogP 2.33
H-bond donor count 1
H-bond acceptor count 4
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.43
% growth inhibition (Hom. pool) 11.28


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 666570
Download HIP data (tab-delimited text)  (excel)
Gene:BUR6(YER159C)|FD-Score:-5.14|P-value:1.36E-7|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC12(YHR107C)|FD-Score:-4.44|P-value:4.44E-6|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.01||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CIA2(YHR122W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:EPL1(YFL024C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.14||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERG29(YMR134W_p)|FD-Score:-3.11|P-value:9.48E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:LSG1(YGL099W)|FD-Score:5.39|P-value:3.59E-8|Clearance:0.07||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD4(YKL165C)|FD-Score:-3.81|P-value:6.85E-5|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NMT1(YLR195C)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.16||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:RLI1(YDR091C)|FD-Score:-3.76|P-value:8.47E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPP1(YHR062C)|FD-Score:10.2|P-value:1.35E-24|Clearance:3.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT2(YDL007W)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT4(YOR259C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP4(YHR069C)|FD-Score:-3.09|P-value:9.86E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP9(YPR137W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC13(YLR208W)|FD-Score:6.7|P-value:1.07E-11|Clearance:1.31||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:6.84|P-value:3.96E-12|Clearance:0.14||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:-3.32|P-value:4.58E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SNM1(YDR478W)|FD-Score:3.9|P-value:4.90E-5|Clearance:0.02||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPN1(YPR133C)|FD-Score:5.31|P-value:5.41E-8|Clearance:0.27||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP72(YPL210C)|FD-Score:-4.28|P-value:9.28E-6|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TBF1(YPL128C)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.53||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UTP11(YKL099C)|FD-Score:5.04|P-value:2.28E-7|Clearance:1.15||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:-4.03|P-value:2.83E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:BUR6(YER159C)|FD-Score:-5.14|P-value:1.36E-7|Clearance:0||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:CDC12(YHR107C)|FD-Score:-4.44|P-value:4.44E-6|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CEP3(YMR168C)|FD-Score:3.87|P-value:5.34E-5|Clearance:0.01||SGD DESC:Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain Gene:CIA2(YHR122W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B Gene:EPL1(YFL024C)|FD-Score:3.17|P-value:7.61E-4|Clearance:0.14||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERG29(YMR134W_p)|FD-Score:-3.11|P-value:9.48E-4|Clearance:0||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:LSG1(YGL099W)|FD-Score:5.39|P-value:3.59E-8|Clearance:0.07||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:MCD4(YKL165C)|FD-Score:-3.81|P-value:6.85E-5|Clearance:0||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NMT1(YLR195C)|FD-Score:3.33|P-value:4.29E-4|Clearance:0.16||SGD DESC:N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction Gene:RLI1(YDR091C)|FD-Score:-3.76|P-value:8.47E-5|Clearance:0||SGD DESC:Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species Gene:RPP1(YHR062C)|FD-Score:10.2|P-value:1.35E-24|Clearance:3.33||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPT2(YDL007W)|FD-Score:-3.21|P-value:6.62E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle Gene:RPT4(YOR259C)|FD-Score:-3.39|P-value:3.51E-4|Clearance:0||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in degradation of ubiquitinated substrates; contributes preferentially to ERAD; required for spindle pole body duplication; mainly nuclear localization Gene:RRP4(YHR069C)|FD-Score:-3.09|P-value:9.86E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:RRP9(YPR137W)|FD-Score:-3.69|P-value:1.11E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, associated with U3 snRNP; component of small ribosomal subunit (SSU) processosome; ortholog of the human U3-55k protein Gene:SEC13(YLR208W)|FD-Score:6.7|P-value:1.07E-11|Clearance:1.31||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SEC31(YDL195W)|FD-Score:6.84|P-value:3.96E-12|Clearance:0.14||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SMC4(YLR086W)|FD-Score:-3.32|P-value:4.58E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate Gene:SNM1(YDR478W)|FD-Score:3.9|P-value:4.90E-5|Clearance:0.02||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPN1(YPR133C)|FD-Score:5.31|P-value:5.41E-8|Clearance:0.27||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP72(YPL210C)|FD-Score:-4.28|P-value:9.28E-6|Clearance:0||SGD DESC:Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane Gene:TBF1(YPL128C)|FD-Score:3.87|P-value:5.53E-5|Clearance:0.53||SGD DESC:Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation Gene:UTP11(YKL099C)|FD-Score:5.04|P-value:2.28E-7|Clearance:1.15||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:YDL196W(YDL196W_d)|FD-Score:-4.03|P-value:2.83E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 666570
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AIM17(YHL021C)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM18(YHR198C)|FD-Score:6.86|P-value:3.50E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARC18(YLR370C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVL9(YLR114C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BUD31(YCR063W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BUR2(YLR226W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CCS1(YMR038C)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLU1(YMR012W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COT1(YOR316C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX18(YGR062C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CRZ1(YNL027W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:DAL81(YIR023W)|FD-Score:9.51|P-value:9.37E-22||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DID4(YKL002W)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOM34(YNL001W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM19(YLR390W)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFG1(YGR271C-A)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EMC5(YIL027C)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EXG2(YDR261C)|FD-Score:6.72|P-value:8.91E-12||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FMP43(YGR243W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GID8(YMR135C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GIT1(YCR098C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLR1(YPL091W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPR1(YDL035C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAL9(YOL089C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HAP4(YKL109W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HIT1(YJR055W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOP2(YGL033W)|FD-Score:-5.84|P-value:2.54E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IFM1(YOL023W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IMO32(YGR031W)|FD-Score:-5.03|P-value:2.50E-7||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:JEN1(YKL217W)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KAP120(YPL125W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KTR6(YPL053C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MCR1(YKL150W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MED1(YPR070W)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET32(YDR253C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MHF1(YOL086W-A)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MPD2(YOL088C)|FD-Score:6.47|P-value:4.88E-11||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRP1(YDR347W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP21(YBL090W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPS28(YDR337W)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTG1(YMR097C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MZM1(YDR493W)|FD-Score:7.13|P-value:4.97E-13||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NOP13(YNL175C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NUM1(YDR150W)|FD-Score:5.38|P-value:3.73E-8||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP2(YLR335W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PEX32(YBR168W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO84(YML123C)|FD-Score:5.82|P-value:3.03E-9||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PMT2(YAL023C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRO2(YOR323C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REG1(YDR028C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RMD5(YDR255C)|FD-Score:4|P-value:3.21E-5||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:ROM2(YLR371W)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPB9(YGL070C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPI1(YIL119C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL13B(YMR142C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RSR1(YGR152C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS5(YOR213C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SEH1(YGL100W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLI1(YGR212W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM6(YBR266C_d)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLS1(YLR139C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SRL3(YKR091W)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:STL1(YDR536W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TDH1(YJL052W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TMA23(YMR269W)|FD-Score:4.57|P-value:2.49E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPN1(YGL186C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:13.7|P-value:4.17E-43||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:20.5|P-value:2.74E-93||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:12.1|P-value:4.25E-34||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSL1(YML100W)|FD-Score:-6.25|P-value:2.08E-10||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TSR2(YLR435W)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBC13(YDR092W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UMP1(YBR173C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:URA2(YJL130C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VAM6(YDL077C)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VID24(YBR105C)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA5(YKL080W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YAF9(YNL107W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YDR008C(YDR008C_d)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR194W-A(YDR194W-A_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFT2(YDR319C_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL214W(YGL214W_d)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR064W(YGR064W_d)|FD-Score:6.72|P-value:8.83E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL086C(YIL086C_d)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL100W(YIL100W_d)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YLL020C(YLL020C_d)|FD-Score:4.38|P-value:5.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML122C(YML122C_d)|FD-Score:5.2|P-value:9.98E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:6.54|P-value:3.01E-11||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL118C(YOL118C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Putative protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR072W(YOR072W_d)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL205C(YPL205C_d)|FD-Score:5.25|P-value:7.67E-8||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPT11(YNL304W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YUR1(YJL139C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication Gene:ADH2(YMR303C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AIM17(YHL021C)|FD-Score:-3.82|P-value:6.68E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM18(YHR198C)|FD-Score:6.86|P-value:3.50E-12||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALG6(YOR002W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease Gene:ARC18(YLR370C)|FD-Score:-3.22|P-value:6.37E-4||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARO1(YDR127W)|FD-Score:6.17|P-value:3.44E-10||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO3(YDR035W)|FD-Score:4.53|P-value:2.99E-6||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:AVL9(YLR114C)|FD-Score:4.13|P-value:1.82E-5||SGD DESC:Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:BUD31(YCR063W)|FD-Score:-4.04|P-value:2.71E-5||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:BUR2(YLR226W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Cyclin for the Sgv1p (Bur1p) protein kinase; Sgv1p and Bur2p comprise a CDK-cyclin complex involved in transcriptional regulation through its phosphorylation of the carboxy-terminal domain of the largest subunit of RNA polymerase II Gene:CCS1(YMR038C)|FD-Score:5.49|P-value:2.03E-8||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CLU1(YMR012W)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:COT1(YOR316C)|FD-Score:3.33|P-value:4.36E-4||SGD DESC:Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistance to cobalt and rhodium; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication Gene:COX18(YGR062C)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Mitochondrial integral inner membrane protein required for membrane insertion of C-terminus of Cox2p; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC Gene:CRZ1(YNL027W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress Gene:DAL81(YIR023W)|FD-Score:9.51|P-value:9.37E-22||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DCS1(YLR270W)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DID4(YKL002W)|FD-Score:5.25|P-value:7.55E-8||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:DOM34(YNL001W)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication Gene:DSE2(YHR143W)|FD-Score:3.22|P-value:6.41E-4||SGD DESC:Daughter cell-specific secreted protein with similarity to glucanases, degrades cell wall from the daughter side causing daughter to separate from mother; expression is repressed by cAMP Gene:ECM19(YLR390W)|FD-Score:4.46|P-value:4.07E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:EFG1(YGR271C-A)|FD-Score:5.94|P-value:1.42E-9||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EMC5(YIL027C)|FD-Score:5.84|P-value:2.66E-9||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:EXG2(YDR261C)|FD-Score:6.72|P-value:8.91E-12||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FMP43(YGR243W)|FD-Score:3.23|P-value:6.15E-4||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FUI1(YBL042C)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress Gene:FYV1(YDR024W_d)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin Gene:GCN3(YKR026C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GID8(YMR135C)|FD-Score:3.54|P-value:2.00E-4||SGD DESC:Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START Gene:GIT1(YCR098C)|FD-Score:-3.13|P-value:8.82E-4||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GLR1(YPL091W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GPR1(YDL035C)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:HAL9(YOL089C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication Gene:HAP4(YKL109W)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HIT1(YJR055W)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HOP2(YGL033W)|FD-Score:-5.84|P-value:2.54E-9||SGD DESC:Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair Gene:IFM1(YOL023W)|FD-Score:3.99|P-value:3.32E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IMO32(YGR031W)|FD-Score:-5.03|P-value:2.50E-7||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:JEN1(YKL217W)|FD-Score:-3.85|P-value:5.95E-5||SGD DESC:Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose Gene:KAP120(YPL125W)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p Gene:KTR6(YPL053C)|FD-Score:3.27|P-value:5.43E-4||SGD DESC:Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MCR1(YKL150W)|FD-Score:-3.63|P-value:1.40E-4||SGD DESC:Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis Gene:MED1(YPR070W)|FD-Score:-3.32|P-value:4.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MET32(YDR253C)|FD-Score:-3.49|P-value:2.42E-4||SGD DESC:Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication Gene:MHF1(YOL086W-A)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1 Gene:MPD2(YOL088C)|FD-Score:6.47|P-value:4.88E-11||SGD DESC:Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p Gene:MRP1(YDR347W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein Gene:MRP21(YBL090W)|FD-Score:3.12|P-value:9.13E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPS28(YDR337W)|FD-Score:4.39|P-value:5.64E-6||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:5.91|P-value:1.69E-9||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:MTG1(YMR097C)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Putative GTPase peripheral to the mitochondrial inner membrane, essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals Gene:MZM1(YDR493W)|FD-Score:7.13|P-value:4.97E-13||SGD DESC:Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog Gene:NOP13(YNL175C)|FD-Score:3.99|P-value:3.28E-5||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:NST1(YNL091W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1 Gene:NUM1(YDR150W)|FD-Score:5.38|P-value:3.73E-8||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:NUP2(YLR335W)|FD-Score:3.5|P-value:2.35E-4||SGD DESC:Nucleoporin involved in nucleocytoplasmic transport, binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization Gene:PEX32(YBR168W)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p Gene:PHO84(YML123C)|FD-Score:5.82|P-value:3.03E-9||SGD DESC:High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity Gene:PMT2(YAL023C)|FD-Score:4.55|P-value:2.69E-6||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; acts in a complex with Pmt1p, can instead interact with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication Gene:PPM1(YDR435C)|FD-Score:-3.12|P-value:9.07E-4||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:PRO2(YOR323C)|FD-Score:3.18|P-value:7.35E-4||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:REG1(YDR028C)|FD-Score:4.72|P-value:1.19E-6||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RMD5(YDR255C)|FD-Score:4|P-value:3.21E-5||SGD DESC:Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain Gene:ROM2(YLR371W)|FD-Score:5.03|P-value:2.51E-7||SGD DESC:GDP/GTP exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p Gene:RPB9(YGL070C)|FD-Score:4.67|P-value:1.51E-6||SGD DESC:RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription Gene:RPI1(YIL119C)|FD-Score:-3.21|P-value:6.75E-4||SGD DESC:Transcription factor with allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF Gene:RPL13B(YMR142C)|FD-Score:3.39|P-value:3.55E-4||SGD DESC:Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication Gene:RPL19A(YBR084C-A)|FD-Score:3.13|P-value:8.80E-4||SGD DESC:Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:3.25|P-value:5.78E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RSR1(YGR152C)|FD-Score:3.43|P-value:2.99E-4||SGD DESC:GTP-binding protein of the ras superfamily required for bud site selection, morphological changes in response to mating pheromone, and efficient cell fusion; localized to the plasma membrane; significantly similar to mammalian Rap GTPases Gene:SAS5(YOR213C)|FD-Score:-3.61|P-value:1.54E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SEH1(YGL100W)|FD-Score:5.45|P-value:2.54E-8||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SLI1(YGR212W)|FD-Score:3.21|P-value:6.71E-4||SGD DESC:N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin Gene:SLM6(YBR266C_d)|FD-Score:3.5|P-value:2.29E-4||SGD DESC:Protein with a potential role in actin cytoskeleton organization; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase Gene:SLS1(YLR139C)|FD-Score:3.28|P-value:5.11E-4||SGD DESC:Mitochondrial membrane protein that coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery Gene:SRL3(YKR091W)|FD-Score:4.68|P-value:1.47E-6||SGD DESC:Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate Gene:STL1(YDR536W)|FD-Score:3.86|P-value:5.59E-5||SGD DESC:Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock Gene:TDH1(YJL052W)|FD-Score:-4.04|P-value:2.68E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TMA23(YMR269W)|FD-Score:4.57|P-value:2.49E-6||SGD DESC:Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan Gene:TPN1(YGL186C)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TRP1(YDR007W)|FD-Score:3.74|P-value:9.23E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:13.7|P-value:4.17E-43||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:20.5|P-value:2.74E-93||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:12.1|P-value:4.25E-34||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TSL1(YML100W)|FD-Score:-6.25|P-value:2.08E-10||SGD DESC:Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication Gene:TSR2(YLR435W)|FD-Score:3.91|P-value:4.52E-5||SGD DESC:Protein with a potential role in pre-rRNA processing Gene:UBC13(YDR092W)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus Gene:UMP1(YBR173C)|FD-Score:3.16|P-value:7.98E-4||SGD DESC:Short-lived chaperone required for correct maturation of the 20S proteasome; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly Gene:URA2(YJL130C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:VAM6(YDL077C)|FD-Score:4.34|P-value:7.16E-6||SGD DESC:Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p Gene:VID24(YBR105C)|FD-Score:-5.15|P-value:1.29E-7||SGD DESC:GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles Gene:VMA5(YKL080W)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Subunit C of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPS52(YDR484W)|FD-Score:-3.17|P-value:7.60E-4||SGD DESC:Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin Gene:YAF9(YNL107W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of both the NuA4 histone H4 acetyltransferase complex and the SWR1 complex, may function to antagonize silencing near telomeres; interacts directly with Swc4p, has homology to human leukemogenic protein AF9, contains a YEATS domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.7|P-value:1.10E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YDR008C(YDR008C_d)|FD-Score:5.27|P-value:6.92E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR056C(YDR056C_p)|FD-Score:3.54|P-value:1.98E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein Gene:YDR194W-A(YDR194W-A_p)|FD-Score:-3.17|P-value:7.57E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YFT2(YDR319C_p)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YGL214W(YGL214W_d)|FD-Score:3.73|P-value:9.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C Gene:YGR064W(YGR064W_d)|FD-Score:6.72|P-value:8.83E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C Gene:YIL086C(YIL086C_d)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL100W(YIL100W_d)|FD-Score:-3.97|P-value:3.58E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A Gene:YLL020C(YLL020C_d)|FD-Score:4.38|P-value:5.82E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1 Gene:YML122C(YML122C_d)|FD-Score:5.2|P-value:9.98E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR166C(YMR166C_p)|FD-Score:6.54|P-value:3.01E-11||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR230W-A(YMR230W-A_p)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Putative protein of unknown function Gene:YOL013W-A(YOL013W-A_p)|FD-Score:-3.28|P-value:5.13E-4||SGD DESC:Putative protein of unknown function; identified by SAGE Gene:YOL118C(YOL118C_d)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR012W(YOR012W_p)|FD-Score:-5.1|P-value:1.73E-7||SGD DESC:Putative protein of unknown function Gene:YOR034C-A(YOR034C-A_p)|FD-Score:3.19|P-value:7.07E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOR072W(YOR072W_d)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL205C(YPL205C_d)|FD-Score:5.25|P-value:7.67E-8||SGD DESC:Hypothetical protein; deletion of locus affects telomere length Gene:YPT11(YNL304W)|FD-Score:-3.78|P-value:7.93E-5||SGD DESC:Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells Gene:YUR1(YJL139C)|FD-Score:-4.11|P-value:1.96E-5||SGD DESC:Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C10.201.35E-243.33RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YDL195W6.843.96E-120.14SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YLR208W6.701.07E-111.31SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGL099W5.393.59E-80.07LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YPR133C5.315.41E-80.27SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YKL099C5.042.28E-71.15UTP11Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YDR478W3.904.90E-50.02SNM1Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
YMR168C3.875.34E-50.01CEP3Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
YPL128C3.875.53E-50.53TBF1Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation
YLR195C3.334.29E-40.16NMT1N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
YFL024C3.177.61E-40.14EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb
YDL143W3.030.001220.04CCT4Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YLR106C2.990.001390.01MDN1Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
YJR006W2.980.001420.01POL31Subunit of DNA polymerase delta (polymerase III); essential for cell viability; involved in DNA replication and DNA repair
YLR223C2.970.001480.14IFH1Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKL211C20.502.74E-93TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YER090W13.704.17E-43TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YDR354W12.104.25E-34TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YIR023W9.519.37E-22DAL81Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
YDR493W7.134.97E-13MZM1Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; human LYRM7 is a functional ortholog
YHR198C6.863.50E-12AIM18Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
YGR064W_d6.728.83E-12YGR064W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
YDR261C6.728.91E-12EXG2Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
YMR166C_p6.543.01E-11YMR166C_pPredicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
YOL088C6.474.88E-11MPD2Member of the protein disulfide isomerase (PDI) family, exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
YDR127W6.173.44E-10ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YGR271C-A5.941.42E-9EFG1Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
YHR120W5.911.69E-9MSH1DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
YIL027C5.842.66E-9EMC5Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
YML123C5.823.03E-9PHO84High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity

GO enrichment analysis for SGTC_2296
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.3839.87E-206SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.015625
0.3703.47E-191SGTC_15412',3',6-trimethoxyflavone 64.0 μMTimTec (Pure natural product library)6888010.0921053
0.3562.51E-176SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0675676
0.3512.52E-170SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0434783
0.3494.15E-169SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.179104
0.3364.54E-156SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.0985916
0.3358.94E-155SGTC_20935331342 194.4 μMChembridge (Fragment library)338390.101449
0.3341.95E-153SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.3311.73E-150SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0945946
0.3305.11E-150SGTC_1909st060837 51.5 μMTimTec (Natural product derivative library)6789370.109589
0.3271.42E-147SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0441176
0.3267.76E-146SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.123077
0.3243.42E-144SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.135135
0.3181.95E-138SGTC_24885268135 45.7 μMMiscellaneous12703560.136364
0.3124.00E-133SGTC_8230417-1665 178.0 μMChemDiv (Drug-like library)34298560.0674157

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_23799035213200 μM0.490566646551Chembridge (Fragment library)240.300342.32213
SGTC_235890371574 μM0.2833332388925Chembridge (Fragment library)231.250520.20513
SGTC_10493226-013466 μM0.2380955702385ChemDiv (Drug-like library)239.226163.00304
SGTC_1695st02691434.5 μM0.2236843289191TimTec (Natural product derivative library)380.43855.26814
SGTC_2102537781672.76 μM0.2166671379702Chembridge (Fragment library)195.19521.33714
SGTC_8790831-1030162 μM0.2142861549252ChemDiv (Drug-like library)240.253983.47103
SGTC_22226885208172.14 μM0.213115609514Chembridge (Fragment library)233.26311.43313
SGTC_21725754775163.92 μM0.2096772263357Chembridge (Fragment library)257.327561.72113
SGTC_1520flindersine88 μM0.20312568230TimTec (Pure natural product library)227.258521.70412
SGTC_22206782180200 μM0.203125833803Chembridge (Fragment library)207.18606-0.26124RNA processing & uracil transport