MPP+

1-methyl-4-phenylpyridin-1-ium

MPP+ (1-methyl-4-phenylpyridinium) is a toxic positively charged molecule and acts by interfering with oxidative phosphorylation in mitochondria, causing depletion of ATP and cell death.

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PubChem MeSH terms: Herbicides



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_230
Screen concentration 141.7 μM
Source Miscellaneous
PubChem CID 39484
SMILES C[N+]1=CC=C(C=C1)C2=CC=CC=C2
Standardized SMILES C[n+]1ccc(cc1)c2ccccc2
Molecular weight 170.2304
ALogP 2.95
H-bond donor count 0
H-bond acceptor count 0
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.91
% growth inhibition (Hom. pool) 0


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 39484
Download HIP data (tab-delimited text)  (excel)
Gene:CDC36(YDL165W)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.02||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CTR86(YCR054C)|FD-Score:5.64|P-value:8.27E-9|Clearance:0.78||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG8(YMR220W)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.15||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LIP1(YMR298W)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:ORC2(YBR060C)|FD-Score:4.73|P-value:1.12E-6|Clearance:0.15||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PGI1(YBR196C)|FD-Score:4.86|P-value:5.77E-7|Clearance:0.13||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RLP24(YLR009W)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.05||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:3.09|P-value:9.94E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.36||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC39(YLR440C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.05||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEN1(YLR430W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SNU56(YDR240C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:TAF12(YDR145W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.34||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UTP25(YIL091C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGR114C(YGR114C_d)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:CDC36(YDL165W)|FD-Score:3.11|P-value:9.36E-4|Clearance:0.02||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CTR86(YCR054C)|FD-Score:5.64|P-value:8.27E-9|Clearance:0.78||SGD DESC:Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress Gene:ERG8(YMR220W)|FD-Score:3.8|P-value:7.25E-5|Clearance:0.15||SGD DESC:Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:LIP1(YMR298W)|FD-Score:-3.27|P-value:5.29E-4|Clearance:0||SGD DESC:Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis Gene:LSG1(YGL099W)|FD-Score:3.88|P-value:5.17E-5|Clearance:0.08||SGD DESC:Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm Gene:ORC2(YBR060C)|FD-Score:4.73|P-value:1.12E-6|Clearance:0.15||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p Gene:PGI1(YBR196C)|FD-Score:4.86|P-value:5.77E-7|Clearance:0.13||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:RLP24(YLR009W)|FD-Score:3.22|P-value:6.48E-4|Clearance:0.05||SGD DESC:Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp Gene:RPP1(YHR062C)|FD-Score:3.09|P-value:9.94E-4|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RRP45(YDR280W)|FD-Score:4.58|P-value:2.35E-6|Clearance:0.36||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress Gene:SEC39(YLR440C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0.05||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SEN1(YLR430W)|FD-Score:-3.22|P-value:6.42E-4|Clearance:0||SGD DESC:Presumed helicase and subunit of the Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3' end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; has a separate role in coordinating DNA replication with transcription, by associating with moving replication forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, which is implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of ALS Gene:SNU56(YDR240C)|FD-Score:3.16|P-value:7.77E-4|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex Gene:TAF12(YDR145W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.34||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:UTP25(YIL091C)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.11||SGD DESC:Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis Gene:YGR114C(YGR114C_d)|FD-Score:3.54|P-value:2.00E-4|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 39484
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABZ2(YMR289W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADE17(YMR120C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APE3(YBR286W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:CDH1(YGL003C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COQ4(YDR204W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CTA1(YDR256C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTM1(YHR109W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CUE1(YMR264W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DIA4(YHR011W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFM1(YHL039W)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:GLY1(YEL046C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HPA2(YPR193C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST1(YOL068C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IRC4(YDR540C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRC7(YFR055W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISU2(YOR226C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:IXR1(YKL032C)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JNM1(YMR294W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MCM22(YJR135C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MRPL38(YKL170W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRS1(YIR021W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NRG2(YBR066C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OTU2(YHL013C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PTC5(YOR090C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:PUG1(YER185W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:PUT2(YHR037W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFX1(YLR176C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM9(YMR063W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL36A(YMR194W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIA1(YOR137C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SNG1(YGR197C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SNQ2(YDR011W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STE20(YHL007C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SYG1(YIL047C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TAT1(YBR069C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TGS1(YPL157W)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRM10(YOL093W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:5.62|P-value:9.53E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VBA3(YCL069W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHC1(YBR235W_p)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAK1(YJL141C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBR032W(YBR032W_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR134C(YDR134C)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YGR107W(YGR107W_d)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL025C(YIL025C_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL118W(YKL118W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR031W(YLR031W_p)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YLR444C(YLR444C_d)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOL107W(YOL107W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR127W(YPR127W)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ZWF1(YNL241C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress Gene:AAT2(YLR027C)|FD-Score:-3.17|P-value:7.64E-4||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:ABZ2(YMR289W)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ADE17(YMR120C)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine Gene:APE3(YBR286W)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Vacuolar aminopeptidase Y, processed to mature form by Prb1p Gene:CDH1(YGL003C)|FD-Score:3.95|P-value:3.93E-5||SGD DESC:Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p Gene:COQ4(YDR204W)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex Gene:CTA1(YDR256C)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Catalase A, breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation Gene:CTM1(YHR109W)|FD-Score:3.14|P-value:8.59E-4||SGD DESC:Cytochrome c lysine methyltransferase, trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth Gene:CUE1(YMR264W)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication Gene:DIA4(YHR011W)|FD-Score:3.17|P-value:7.69E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:EFM1(YHL039W)|FD-Score:5.02|P-value:2.62E-7||SGD DESC:Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:GLY1(YEL046C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:HPA2(YPR193C)|FD-Score:4.84|P-value:6.50E-7||SGD DESC:Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity Gene:HST1(YOL068C)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and mitotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance Gene:IRC4(YDR540C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRC7(YFR055W_p)|FD-Score:3.37|P-value:3.71E-4||SGD DESC:Beta-lyase involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner Gene:ISU2(YOR226C)|FD-Score:-3.34|P-value:4.17E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:IXR1(YKL032C)|FD-Score:4.77|P-value:9.18E-7||SGD DESC:Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b Gene:JNM1(YMR294W)|FD-Score:-3.37|P-value:3.79E-4||SGD DESC:Component of the yeast dynactin complex, consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B Gene:MCM22(YJR135C)|FD-Score:3.42|P-value:3.08E-4||SGD DESC:Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4 Gene:MRPL38(YKL170W)|FD-Score:4.98|P-value:3.21E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRS1(YIR021W)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Protein required for the splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA Gene:NRG2(YBR066C)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:OTU2(YHL013C)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress Gene:PAC2(YER007W)|FD-Score:5.7|P-value:6.00E-9||SGD DESC:Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl Gene:PTC5(YOR090C)|FD-Score:-3.13|P-value:8.80E-4||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p Gene:PUG1(YER185W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:PUT2(YHR037W)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism Gene:RFX1(YLR176C)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM9(YMR063W)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL36A(YMR194W)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication Gene:SAS5(YOR213C)|FD-Score:3.25|P-value:5.70E-4||SGD DESC:Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity Gene:SIA1(YOR137C)|FD-Score:3.56|P-value:1.86E-4||SGD DESC:Protein of unassigned function involved in activation of the Pma1p plasma membrane H+-ATPase by glucose Gene:SNG1(YGR197C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance Gene:SNQ2(YDR011W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species Gene:STE20(YHL007C)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p Gene:SYG1(YIL047C)|FD-Score:3.81|P-value:6.88E-5||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TAT1(YBR069C)|FD-Score:4.13|P-value:1.79E-5||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:TGS1(YPL157W)|FD-Score:4.77|P-value:9.40E-7||SGD DESC:Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs Gene:TRM10(YOL093W)|FD-Score:-3.29|P-value:4.99E-4||SGD DESC:tRNA methyltransferase; methylates the N-1 position of guanosine in tRNAs; protein abundance increases in response to DNA replication stress Gene:TRP4(YDR354W)|FD-Score:5.62|P-value:9.53E-9||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VBA3(YCL069W)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VHC1(YBR235W_p)|FD-Score:4.37|P-value:6.19E-6||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:YAK1(YJL141C)|FD-Score:3.39|P-value:3.43E-4||SGD DESC:Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose Gene:YBR032W(YBR032W_d)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YBR200W-A(YBR200W-A_p)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR134C(YDR134C)|FD-Score:-3.17|P-value:7.71E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YGR107W(YGR107W_d)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR151C(YGR151C_d)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress Gene:YIL025C(YIL025C_d)|FD-Score:3.34|P-value:4.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YJL120W(YJL120W_d)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YKL118W(YKL118W_d)|FD-Score:3.31|P-value:4.74E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YLR031W(YLR031W_p)|FD-Score:3.78|P-value:7.92E-5||SGD DESC:Putative protein of unknown function Gene:YLR419W(YLR419W_p)|FD-Score:3.79|P-value:7.44E-5||SGD DESC:Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene Gene:YLR444C(YLR444C_d)|FD-Score:4.43|P-value:4.79E-6||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR085W(YMR085W_p)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YMR316C-A(YMR316C-A_p)|FD-Score:3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W Gene:YOL013W-B(YOL013W-B_d)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element Gene:YOL107W(YOL107W_p)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YPL038W-A(YPL038W-A_p)|FD-Score:3.11|P-value:9.22E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPR127W(YPR127W)|FD-Score:3.88|P-value:5.31E-5||SGD DESC:Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ZWF1(YNL241C)|FD-Score:3.09|P-value:9.97E-4||SGD DESC:Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YCR054C5.648.27E-90.78CTR86Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
YBR196C4.865.77E-70.13PGI1Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
YBR060C4.731.12E-60.15ORC2Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
YDR280W4.582.35E-60.36RRP45Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
YDR145W4.221.23E-50.34TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YGL099W3.885.17E-50.08LSG1Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
YMR220W3.807.25E-50.15ERG8Phosphomevalonate kinase, an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
YIL091C3.651.30E-40.11UTP25Nucleolar protein required for 35S pre-RNA processing and 40S ribosomal subunit biogenesis
YGR114C_d3.542.00E-40.32YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR009W3.226.48E-40.05RLP24Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
YDR240C3.167.77E-41.03E-4SNU56Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
YLR440C3.167.77E-40.05SEC39Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
YDL165W3.119.36E-40.02CDC36Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
YHR062C3.099.94E-40.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPL094C3.040.001170.05SEC62Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YER007W5.706.00E-9PAC2Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
YDR354W5.629.53E-9TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YHL039W5.022.62E-7EFM1Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
YKL170W4.983.21E-7MRPL38Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
YHR037W4.963.58E-7PUT2Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism
YPR193C4.846.50E-7HPA2Tetrameric histone acetyltransferase with similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity
YKL032C4.779.18E-7IXR1Protein that binds DNA containing intrastrand cross-links formed by cisplatin, contains two HMG (high mobility group box) domains, which confer the ability to bend cisplatin-modified DNA; mediates aerobic transcriptional repression of COX5b
YPL157W4.779.40E-7TGS1Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
YHL007C4.661.60E-6STE20Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
YLR444C_d4.434.79E-6YLR444C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YBR235W_p4.376.19E-6VHC1_pVacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
YMR264W4.251.09E-5CUE1Ubiquitin-binding protein; endoplasmic reticulum membrane protein that recruits the ubiquitin-conjugating enzyme Ubc7p to the ER where it functions in protein degradation; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination; CUE1 has a paralog, CUE4, that arose from the whole genome duplication
YBR069C4.131.79E-5TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YGL003C3.953.93E-5CDH1Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
YMR085W_p3.904.85E-5YMR085W_pPutative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1

GO enrichment analysis for SGTC_230
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1454.51E-29SGTC_264promethazine 12.5 mMMiscellaneous60140.15DNA intercalators
0.1188.74E-20SGTC_4711-acyl-paf 4.0 μMICCB bioactive library167593670.015873DNA intercalators
0.1031.73E-15SGTC_229benomyl 22.9 μMMiscellaneous287800.109091tubulin folding & SWR complex
0.1016.82E-15SGTC_2727riluzole 51.8 μMMiscellaneous50700.0666667mitochondrial processes
0.0937.02E-13SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.046875
0.0921.36E-12SGTC_286k060-0067 22.9 μMChemDiv (Drug-like library)59850510.107143tubulin folding & SWR complex
0.0911.96E-12SGTC_1444161-2749 112.4 μMChemDiv (Drug-like library)7975010.1DNA intercalators
0.0903.81E-12SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0769231plasma membrane duress
0.0905.43E-12SGTC_407eburnamonine 100.0 μMMiscellaneous31950.101695
0.0895.99E-12SGTC_512prima-1 270.0 μMICCB bioactive library3229680.025DNA intercalators
0.0857.24E-11SGTC_257daunorubicin 18.5 μMMiscellaneous303230.0617284anthracycline transcription coupled DNA repair
0.0841.04E-10SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.175RPP1 & pyrimidine depletion
0.0841.08E-10SGTC_1612st002233 45.4 μMTimTec (Natural product derivative library)10350210.114754
0.0814.05E-10SGTC_6360kpi-0074 126.0 μMChemDiv (Drug-like library)6164340.047619
0.0791.34E-9SGTC_9332914-0560 114.0 μMChemDiv (Drug-like library)4638870.0930233

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_550767-02856.56 μM0.3461543116166ChemDiv (Drug-like library)325.446086.93500
SGTC_3590081-005815.9 μM0.3428573494159ChemDiv (Drug-like library)250.35814.1020060S ribosome export
SGTC_1069diphenylcyclopropenone8.49 μM0.33333365057NIH Clinical Collection206.23933.30101
SGTC_630099-024226.7 μM0.303033898502ChemDiv (Drug-like library)265.372744.0501mitochondrial stress
SGTC_15091326-113180 μM0.30303768313ChemDiv (Drug-like library)239.312283.09402
SGTC_2970986-003224 μM0.32847425ChemDiv (Drug-like library)338.42175.6010160S ribosome export
SGTC_6090986-002686.3 μM0.32848610ChemDiv (Drug-like library)302.38965.17901calcium & mitochondrial duress
SGTC_117lucigenin22.08 μM0.29629665100ChemDiv (Drug-like library)386.487687.35600redox potentiating
SGTC_6010986-002962.9 μM0.29268324747981ChemDiv (Drug-like library)367.259085.44101calcium & mitochondrial duress
SGTC_400327-010319.75 μM0.282051409036ChemDiv (Drug-like library)324.395125.11701