b-estradiol

13-methyl-6,7,8,9,11,12,14,15,16,17-decahydrocyclopenta[a]phenanthrene-3,17-diol




Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_231
Screen concentration 524.0 μM
Source Miscellaneous
PubChem CID 450
SMILES CC12CCC3C(C1CCC2O)CCC4=C3C=CC(=C4)O
Standardized SMILES CC12CCC3C(CCc4cc(O)ccc34)C1CCC2O
Molecular weight 272.382
ALogP 3.84
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.28
% growth inhibition (Hom. pool) 6.29


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 450
Download HIP data (tab-delimited text)  (excel)
Gene:NOP56(YLR197W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.22||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PSF3(YOL146W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPL17A(YKL180W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.22||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRN11(YML043C)|FD-Score:-3.11|P-value:9.51E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC11(YIR022W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.22||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SPP381(YBR152W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.15||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAF12(YDR145W)|FD-Score:3.8|P-value:7.19E-5|Clearance:0.22||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YGR114C(YGR114C_d)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:NOP56(YLR197W)|FD-Score:3.37|P-value:3.71E-4|Clearance:0.22||SGD DESC:Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects Gene:PSF3(YOL146W)|FD-Score:-3.12|P-value:9.16E-4|Clearance:0||SGD DESC:Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery Gene:RPL17A(YKL180W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.22||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRN11(YML043C)|FD-Score:-3.11|P-value:9.51E-4|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC11(YIR022W)|FD-Score:3.62|P-value:1.50E-4|Clearance:0.22||SGD DESC:18kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum Gene:SPP381(YBR152W)|FD-Score:3.12|P-value:9.13E-4|Clearance:0.15||SGD DESC:mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p Gene:TAF12(YDR145W)|FD-Score:3.8|P-value:7.19E-5|Clearance:0.22||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:YGR114C(YGR114C_d)|FD-Score:3.34|P-value:4.18E-4|Clearance:0.22||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 450
Download HOP data (tab-delimited text)  (excel)
Gene:ADR1(YDR216W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ATP14(YLR295C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CUP2(YGL166W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DPH2(YKL191W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:ECM2(YBR065C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM7(YLR443W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FAR11(YNL127W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GLY1(YEL046C)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:IMP2(YMR035W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:MAM1(YER106W)|FD-Score:4.76|P-value:9.90E-7||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MCX1(YBR227C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MNN1(YER001W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MSC6(YOR354C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PCL8(YPL219W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PMS1(YNL082W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUG1(YER185W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:SAC1(YKL212W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP155(YFR040W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SBP1(YHL034C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SOP4(YJL192C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRF1(YDL133W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYG1(YIL047C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TAL1(YLR354C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:VBA3(YCL069W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VID22(YLR373C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YEA4(YEL004W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YER152C(YER152C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YJL150W(YJL150W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMR103C(YMR103C_d)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR109W(YPR109W_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPT53(YNL093W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ADR1(YDR216W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Carbon source-responsive zinc-finger transcription factor, required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization Gene:ATP14(YLR295C)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress Gene:CUP2(YGL166W)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DAL80(YKR034W)|FD-Score:4.14|P-value:1.73E-5||SGD DESC:Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication Gene:DPH2(YKL191W)|FD-Score:-3.11|P-value:9.27E-4||SGD DESC:Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p Gene:ECM2(YBR065C)|FD-Score:-3.61|P-value:1.52E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ECM7(YLR443W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency Gene:FAR11(YNL127W)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GLY1(YEL046C)|FD-Score:4.96|P-value:3.58E-7||SGD DESC:Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis Gene:IMP2(YMR035W)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p Gene:MAM1(YER106W)|FD-Score:4.76|P-value:9.90E-7||SGD DESC:Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle Gene:MCX1(YBR227C)|FD-Score:3.27|P-value:5.47E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MNN1(YER001W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family Gene:MSC6(YOR354C)|FD-Score:3.13|P-value:8.65E-4||SGD DESC:Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PCL8(YPL219W)|FD-Score:3.16|P-value:7.93E-4||SGD DESC:Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation Gene:PMS1(YNL082W)|FD-Score:-3.24|P-value:6.05E-4||SGD DESC:ATP-binding protein required for mismatch repair in mitosis and meiosis; functions as a heterodimer with Mlh1p, binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL Gene:PUG1(YER185W)|FD-Score:-3.26|P-value:5.56E-4||SGD DESC:Plasma membrane protein with roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins Gene:SAC1(YKL212W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SAP155(YFR040W)|FD-Score:3.18|P-value:7.29E-4||SGD DESC:Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress Gene:SBP1(YHL034C)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SOP4(YJL192C)|FD-Score:3.27|P-value:5.38E-4||SGD DESC:ER-membrane protein; suppressor of pma1-7, deletion of SOP4 slows down the export of wild-type Pma1p and Pma1-7 from the ER Gene:SRF1(YDL133W)|FD-Score:3.92|P-value:4.45E-5||SGD DESC:Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients Gene:SSD1(YDR293C)|FD-Score:3.24|P-value:5.88E-4||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SYG1(YIL047C)|FD-Score:3.36|P-value:3.95E-4||SGD DESC:Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency Gene:TAL1(YLR354C)|FD-Score:3.56|P-value:1.87E-4||SGD DESC:Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate Gene:VBA3(YCL069W)|FD-Score:4.65|P-value:1.66E-6||SGD DESC:Permease of basic amino acids in the vacuolar membrane Gene:VID22(YLR373C)|FD-Score:3.28|P-value:5.22E-4||SGD DESC:Glycosylated integral membrane protein localized to the plasma membrane; plays a role in fructose-1,6-bisphosphatase (FBPase) degradation; involved in FBPase transport from the cytosol to Vid (vacuole import and degradation) vesicles Gene:YEA4(YEL004W)|FD-Score:-3.37|P-value:3.70E-4||SGD DESC:Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER Gene:YER152C(YER152C)|FD-Score:3.92|P-value:4.49E-5||SGD DESC:Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene Gene:YJL150W(YJL150W_d)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKR075C(YKR075C_p)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Protein of unknown function; similar to YOR062Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS Gene:YMR103C(YMR103C_d)|FD-Score:-3.79|P-value:7.45E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR109W(YPR109W_p)|FD-Score:3.31|P-value:4.63E-4||SGD DESC:Predicted membrane protein; dipoid deletion strain has high budding index Gene:YPT53(YNL093W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR145W3.807.19E-50.22TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YIR022W3.621.50E-40.22SEC1118kDa catalytic subunit of the Signal Peptidase Complex (SPC; Spc1p, Spc2p, Spc3p, and Sec11p) which cleaves the signal sequence of proteins targeted to the endoplasmic reticulum
YKL180W3.462.68E-40.22RPL17ARibosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
YLR197W3.373.71E-40.22NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YGR114C_d3.344.18E-40.22YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YBR152W3.129.13E-40.15SPP381mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
YGL201C2.960.001520.04MCM6Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
YBL105C2.930.001710.07PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YGR280C2.860.002130.11PXR1Essential protein involved in rRNA and snoRNA maturation; competes with TLC1 RNA for binding to Est2p, suggesting a role in negative regulation of telomerase; human homolog inhibits telomerase; contains a G-patch RNA interacting domain
YKL165C2.740.003030.02MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YDR045C2.720.003220.08RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YGL112C2.650.004050.05TAF6Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
YMR290W-A_d2.600.004700.00YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YMR117C2.590.004740.02SPC24Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
YPR162C2.570.005020.01ORC4Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YEL046C4.963.58E-7GLY1Threonine aldolase, catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
YER106W4.769.90E-7MAM1Monopolin, kinetochore associated protein involved in chromosome attachment to meiotic spindle
YNL093W4.681.46E-6YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YCL069W4.651.66E-6VBA3Permease of basic amino acids in the vacuolar membrane
YKR034W4.141.73E-5DAL80Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
YLR295C3.953.97E-5ATP14Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
YNL127W3.934.26E-5FAR11Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2
YDL133W3.924.45E-5SRF1Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
YER152C3.924.49E-5YER152CProtein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
YHL034C3.601.57E-4SBP1Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11
YLR354C3.561.87E-4TAL1Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate
YKL212W3.462.67E-4SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YLR443W3.452.80E-4ECM7Non-essential putative integral membrane protein with a role in calcium uptake; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
YER001W3.383.57E-4MNN1Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
YIL047C3.363.95E-4SYG1Plasma membrane protein of unknown function; truncation and overexpression suppresses lethality of G-alpha protein deficiency

GO enrichment analysis for SGTC_231
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0872.39E-11SGTC_31719103192 49.5 μMChembridge (Drug-like library)130184340.0684932
0.0714.18E-8SGTC_30749116018 49.5 μMChembridge (Drug-like library)170273030.075RNA processing & uracil transport
0.0715.71E-8SGTC_6653914-0008 307.0 μMChemDiv (Drug-like library)10695490.0833333
0.0672.86E-7SGTC_21345324836 1.5 μMChembridge (Fragment library)6992720.0645161
0.0656.58E-7SGTC_1887st057648 70.8 μMTimTec (Natural product derivative library)6886720.0704225
0.0657.00E-7SGTC_23047443016 91.4 μMChembridge (Fragment library)9398540.0704225
0.0647.14E-7SGTC_230MPP+ 141.7 μMMiscellaneous394840.0754717
0.0647.21E-7SGTC_2734clemastine 41.6 μMMiscellaneous269870.0933333ERG2
0.0631.22E-6SGTC_397arbidol 50.0 μMMiscellaneous1314100.0777778cell wall
0.0622.00E-6SGTC_22997976368 114.9 μMChembridge (Fragment library)29797180.0757576
0.0622.17E-6SGTC_233rapamycin 362.0 pMMiscellaneous52846160.0676692
0.0613.11E-6SGTC_28959054447 58.4 μMChembridge (Drug-like library)26974500.0547945
0.0604.03E-6SGTC_25992',3-dihydroxy-4,4',6'-trimethoxychalcone 100.0 μMMicrosource (Natural product library)53790710.0972222
0.0604.62E-6SGTC_7070865-0008 204.0 μMChemDiv (Drug-like library)7230430.0461538DNA damage response
0.0596.27E-6SGTC_1993st073995 70.8 μMTimTec (Natural product derivative library)30983940.116883

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_406estrone100 μM0.625870Miscellaneous270.366083.93812
SGTC_1519esterone74 μM0.62698TimTec (Pure natural product library)270.366083.93812DNA intercalators
SGTC_417st075190100 μM0.603448222757TimTec (Pure natural product library)376.488025.51213
SGTC_1767st04499270.3 μM0.5849069600693TimTec (Natural product derivative library)284.395963.50123
SGTC_1587estradiol valerate56.1 μM0.58333313791TimTec (Pure natural product library)356.498385.79613
SGTC_416estradiol valerate100 μM0.58333313791TimTec (Pure natural product library)356.498385.79613
SGTC_1974st06944826.9 μM0.3684216323587TimTec (Natural product derivative library)305.454943.36923
SGTC_2678equilin100 μM0.338713247Microsource (Natural product library)268.35023.68812
SGTC_25303-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene92.62 μM0.2586216708760Microsource (Natural product library)216.31873.35611
SGTC_1572epiandrosterone68.9 μM0.258065441302TimTec (Pure natural product library)290.44033.58812
SGTC_2540epiandrosterone100 μM0.258065441302TimTec (Pure natural product library)290.44033.58812
SGTC_2584androsterone48.74 μM0.2580652754130Microsource (Natural product library)290.44033.58812cell wall