7706453

1-[(3-methylphenyl)methoxy]-4-oxidoquinoxalin-4-ium-2-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2312
Screen concentration 155.7 μM
Source Chembridge (Fragment library)
PubChem CID 976098
SMILES CC1=CC(=CC=C1)CON2C3=CC=CC=C3[N+](=CC2=O)[O-]
Standardized SMILES Cc1cccc(CON2C(=O)C=[N+]([O-])c3ccccc23)c1
Molecular weight 282.294
ALogP 1.95
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 11.06
% growth inhibition (Hom. pool) 6.95


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 976098
Download HIP data (tab-delimited text)  (excel)
Gene:ALA1(YOR335C)|FD-Score:4.36|P-value:6.62E-6|Clearance:0.16||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET4(YJL031C)|FD-Score:5.03|P-value:2.40E-7|Clearance:0.48||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CNS1(YBR155W)|FD-Score:3.99|P-value:3.27E-5|Clearance:0.01||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:CWC2(YDL209C)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DPB11(YJL090C)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.1||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ERG9(YHR190W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.08||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:ESA1(YOR244W)|FD-Score:3.98|P-value:3.42E-5|Clearance:0.06||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GLC7(YER133W)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.03||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.01||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HYP2(YEL034W)|FD-Score:4.09|P-value:2.17E-5|Clearance:0.06||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MCD4(YKL165C)|FD-Score:9.68|P-value:1.92E-22|Clearance:4.64||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NET1(YJL076W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.09||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NNF1(YJR112W)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.01||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOC2(YOR206W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.15||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:ORC1(YML065W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.08||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PMI40(YER003C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.05||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:POP8(YBL018C)|FD-Score:-3.84|P-value:6.09E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP39(YML046W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.14||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RER2(YBR002C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.01||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIX1(YHR197W)|FD-Score:-4.1|P-value:2.05E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:SEC13(YLR208W)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.09||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SME1(YOR159C)|FD-Score:4.51|P-value:3.29E-6|Clearance:0.15||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SNU71(YGR013W)|FD-Score:-3.94|P-value:4.02E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSL1(YLR005W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.01||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TAD2(YJL035C)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.05||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TIF34(YMR146C)|FD-Score:-3.72|P-value:9.94E-5|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YLR140W(YLR140W_d)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:ALA1(YOR335C)|FD-Score:4.36|P-value:6.62E-6|Clearance:0.16||SGD DESC:Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog Gene:BET4(YJL031C)|FD-Score:5.03|P-value:2.40E-7|Clearance:0.48||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CNS1(YBR155W)|FD-Score:3.99|P-value:3.27E-5|Clearance:0.01||SGD DESC:TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion Gene:CWC2(YDL209C)|FD-Score:3.15|P-value:8.21E-4|Clearance:0.09||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2 Gene:DPB11(YJL090C)|FD-Score:4.19|P-value:1.39E-5|Clearance:0.1||SGD DESC:DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress Gene:ERG9(YHR190W)|FD-Score:3.7|P-value:1.06E-4|Clearance:0.08||SGD DESC:Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway Gene:ESA1(YOR244W)|FD-Score:3.98|P-value:3.42E-5|Clearance:0.06||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:GLC7(YER133W)|FD-Score:4.03|P-value:2.83E-5|Clearance:0.03||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:HRR25(YPL204W)|FD-Score:3.25|P-value:5.81E-4|Clearance:0.01||SGD DESC:Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) Gene:HYP2(YEL034W)|FD-Score:4.09|P-value:2.17E-5|Clearance:0.06||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:MCD4(YKL165C)|FD-Score:9.68|P-value:1.92E-22|Clearance:4.64||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:NET1(YJL076W)|FD-Score:3.39|P-value:3.46E-4|Clearance:0.09||SGD DESC:Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication Gene:NNF1(YJR112W)|FD-Score:3.99|P-value:3.35E-5|Clearance:0.01||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NOC2(YOR206W)|FD-Score:3.85|P-value:5.86E-5|Clearance:0.15||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-3.52|P-value:2.15E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:ORC1(YML065W)|FD-Score:3.61|P-value:1.54E-4|Clearance:0.08||SGD DESC:Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity Gene:PMI40(YER003C)|FD-Score:3.92|P-value:4.48E-5|Clearance:0.05||SGD DESC:Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation Gene:POP8(YBL018C)|FD-Score:-3.84|P-value:6.09E-5|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:PRP39(YML046W)|FD-Score:3.53|P-value:2.09E-4|Clearance:0.14||SGD DESC:U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats Gene:RER2(YBR002C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.01||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIX1(YHR197W)|FD-Score:-4.1|P-value:2.05E-5|Clearance:0||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene Gene:SEC13(YLR208W)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.09||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:SME1(YOR159C)|FD-Score:4.51|P-value:3.29E-6|Clearance:0.15||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:SNU71(YGR013W)|FD-Score:-3.94|P-value:4.02E-5|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart Gene:SSL1(YLR005W)|FD-Score:3.86|P-value:5.56E-5|Clearance:0.01||SGD DESC:Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p Gene:TAD2(YJL035C)|FD-Score:4.56|P-value:2.61E-6|Clearance:0.05||SGD DESC:Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs Gene:TIF34(YMR146C)|FD-Score:-3.72|P-value:9.94E-5|Clearance:0||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:YLR140W(YLR140W_d)|FD-Score:3.31|P-value:4.71E-4|Clearance:0.06||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 976098
Download HOP data (tab-delimited text)  (excel)
Gene:ADP1(YCR011C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:ARO1(YDR127W)|FD-Score:10.9|P-value:4.92E-28||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP15(YPL271W)|FD-Score:-4.49|P-value:3.52E-6||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AUA1(YFL010W-A)|FD-Score:-7.23|P-value:2.47E-13||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BNI1(YNL271C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CRH1(YGR189C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CSF1(YLR087C)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELC1(YPL046C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG5(YMR015C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:EXO5(YBR163W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FLO1(YAR050W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRA1(YLL029W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GCY1(YOR120W)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HGH1(YGR187C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:ICY2(YPL250C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LGE1(YPL055C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LST7(YGR057C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAL31(YBR298C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MNT4(YNR059W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP20(YDR405W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MUP3(YHL036W)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAB6(YML117W)|FD-Score:-4.99|P-value:3.05E-7||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NBP2(YDR162C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NOP6(YDL213C)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PDR1(YGL013C)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR18(YNR070W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PES4(YFR023W)|FD-Score:5.28|P-value:6.63E-8||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET123(YOR158W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET191(YJR034W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHO23(YNL097C)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PRC1(YMR297W)|FD-Score:-10.5|P-value:3.70E-26||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO1(YDR300C)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REG1(YDR028C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFX1(YLR176C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM9(YMR063W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL27B(YDR471W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:18.7|P-value:4.90E-78||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAE2(YGL175C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SBH1(YER087C-B)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCS7(YMR272C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SIP3(YNL257C)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIT4(YDL047W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKY1(YMR216C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNZ2(YNL333W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SOD1(YJR104C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOL3(YHR163W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPG3(YDR504C)|FD-Score:8.5|P-value:9.85E-18||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPS100(YHR139C)|FD-Score:-4.29|P-value:8.88E-6||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRC1(YML034W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWI4(YER111C)|FD-Score:-4.33|P-value:7.33E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDH2(YJR009C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI72(YOR192C)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOM71(YHR117W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:13.7|P-value:4.23E-43||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.3|P-value:3.62E-35||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.45|P-value:1.44E-17||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TVP18(YMR071C)|FD-Score:5.01|P-value:2.68E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC5(YDR059C)|FD-Score:-4.9|P-value:4.79E-7||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VHR2(YER064C_p)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VHS2(YIL135C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YBL083C(YBL083C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL002C(YCL002C_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.34|P-value:1.08E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR282C(YDR282C_p)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR444W(YDR444W_p)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YER137C(YER137C_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YGL081W(YGL081W_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YHC3(YJL059W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKU80(YMR106C)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YME2(YMR302C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML122C(YML122C_d)|FD-Score:5.08|P-value:1.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function Gene:YNR048W(YNR048W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YNR071C(YNR071C_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR105W(YOR105W_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR225W(YOR225W_d)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSP2(YDR326C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication Gene:ADP1(YCR011C)|FD-Score:3.69|P-value:1.12E-4||SGD DESC:Putative ATP-dependent permease of the ABC transporter family of proteins Gene:ARO1(YDR127W)|FD-Score:10.9|P-value:4.92E-28||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ATP15(YPL271W)|FD-Score:-4.49|P-value:3.52E-6||SGD DESC:Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated Gene:ATP3(YBR039W)|FD-Score:4.94|P-value:3.97E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AUA1(YFL010W-A)|FD-Score:-7.23|P-value:2.47E-13||SGD DESC:Protein required for the negative regulation by ammonia of Gap1p, which is a general amino acid permease Gene:BNI1(YNL271C)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BUD14(YAR014C)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; inhibitor of the actin assembly factor Bnr1p (formin); diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress Gene:CRH1(YGR189C)|FD-Score:-3.59|P-value:1.64E-4||SGD DESC:Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress Gene:CSF1(YLR087C)|FD-Score:6.93|P-value:2.10E-12||SGD DESC:Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:DAL81(YIR023W)|FD-Score:4.71|P-value:1.26E-6||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:ELC1(YPL046C)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair Gene:ERG5(YMR015C)|FD-Score:4.6|P-value:2.15E-6||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:EXO5(YBR163W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:Mitochondrial 5'-3' exonuclease and sliding exonuclease, required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2 Gene:FLO1(YAR050W)|FD-Score:-3.63|P-value:1.41E-4||SGD DESC:Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p Gene:FRA1(YLL029W)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GCY1(YOR120W)|FD-Score:5.01|P-value:2.69E-7||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GLE2(YER107C)|FD-Score:3.45|P-value:2.83E-4||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HGH1(YGR187C)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Nonessential protein of unknown function; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress Gene:ICY2(YPL250C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LGE1(YPL055C)|FD-Score:3.53|P-value:2.07E-4||SGD DESC:Protein of unknown function; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division Gene:LST7(YGR057C)|FD-Score:3.16|P-value:8.02E-4||SGD DESC:Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface Gene:MAL31(YBR298C)|FD-Score:3.89|P-value:5.01E-5||SGD DESC:Maltose permease, high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C Gene:MNT4(YNR059W)|FD-Score:4.15|P-value:1.65E-5||SGD DESC:Putative alpha-1,3-mannosyltransferase, not required for protein O-glycosylation Gene:MRP20(YDR405W)|FD-Score:3.5|P-value:2.30E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL28(YDR462W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress Gene:MST1(YKL194C)|FD-Score:3.32|P-value:4.54E-4||SGD DESC:Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro Gene:MUP3(YHL036W)|FD-Score:-3.1|P-value:9.56E-4||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAB6(YML117W)|FD-Score:-4.99|P-value:3.05E-7||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NBP2(YDR162C)|FD-Score:3.29|P-value:4.95E-4||SGD DESC:Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM) Gene:NOP6(YDL213C)|FD-Score:4.89|P-value:5.09E-7||SGD DESC:rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes Gene:PDR1(YGL013C)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR18(YNR070W_p)|FD-Score:3.49|P-value:2.46E-4||SGD DESC:Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PES4(YFR023W)|FD-Score:5.28|P-value:6.63E-8||SGD DESC:Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication Gene:PET123(YOR158W)|FD-Score:3.47|P-value:2.59E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator Gene:PET191(YJR034W)|FD-Score:-3.45|P-value:2.78E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHO23(YNL097C)|FD-Score:4.48|P-value:3.70E-6||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PRC1(YMR297W)|FD-Score:-10.5|P-value:3.70E-26||SGD DESC:Vacuolar carboxypeptidase Y (proteinase C; CPY), broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in the vacuole; member of the serine carboxypeptidase family Gene:PRO1(YDR300C)|FD-Score:-3.39|P-value:3.43E-4||SGD DESC:Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:3.18|P-value:7.33E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-3.82|P-value:6.54E-5||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:REG1(YDR028C)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:RFX1(YLR176C)|FD-Score:3.22|P-value:6.52E-4||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RIM9(YMR063W)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI; Gene:RPL27B(YDR471W)|FD-Score:-4.19|P-value:1.37E-5||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:18.7|P-value:4.90E-78||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAE2(YGL175C)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents Gene:SBH1(YER087C-B)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SCS7(YMR272C)|FD-Score:3.32|P-value:4.52E-4||SGD DESC:Sphingolipid alpha-hydroxylase, functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth Gene:SIP3(YNL257C)|FD-Score:-3.11|P-value:9.24E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SIT4(YDL047W)|FD-Score:-4.18|P-value:1.45E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SKY1(YMR216C)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNZ2(YNL333W)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:SOD1(YJR104C)|FD-Score:3.51|P-value:2.25E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOL3(YHR163W)|FD-Score:3.3|P-value:4.87E-4||SGD DESC:6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication Gene:SPF1(YEL031W)|FD-Score:4.4|P-value:5.42E-6||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPG3(YDR504C)|FD-Score:8.5|P-value:9.85E-18||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SPS100(YHR139C)|FD-Score:-4.29|P-value:8.88E-6||SGD DESC:Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin Gene:SRC1(YML034W)|FD-Score:4.12|P-value:1.91E-5||SGD DESC:Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance Gene:SSD1(YDR293C)|FD-Score:4.66|P-value:1.58E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWI4(YER111C)|FD-Score:-4.33|P-value:7.33E-6||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:TDH2(YJR009C)|FD-Score:-3.11|P-value:9.51E-4||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; TDH2 has a paralog, TDH3, that arose from the whole genome duplication Gene:THI72(YOR192C)|FD-Score:-3.86|P-value:5.77E-5||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TOM71(YHR117W)|FD-Score:3.3|P-value:4.90E-4||SGD DESC:Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication Gene:TRP1(YDR007W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:13.7|P-value:4.23E-43||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:12.3|P-value:3.62E-35||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:8.45|P-value:1.44E-17||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.24|P-value:6.06E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TVP18(YMR071C)|FD-Score:5.01|P-value:2.68E-7||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments Gene:UBC5(YDR059C)|FD-Score:-4.9|P-value:4.79E-7||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:VHR2(YER064C_p)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:VHS2(YIL135C)|FD-Score:4.06|P-value:2.43E-5||SGD DESC:Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p Gene:YBL083C(YBL083C_d)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3 Gene:YCL002C(YCL002C_p)|FD-Score:3.83|P-value:6.48E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:7.34|P-value:1.08E-13||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR282C(YDR282C_p)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation Gene:YDR444W(YDR444W_p)|FD-Score:-4.16|P-value:1.61E-5||SGD DESC:Putative hydrolase acting on ester bonds Gene:YER137C(YER137C_p)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function Gene:YGL081W(YGL081W_p)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis Gene:YHC3(YJL059W)|FD-Score:3.29|P-value:5.08E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YKU80(YMR106C)|FD-Score:-4.31|P-value:8.10E-6||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YME2(YMR302C)|FD-Score:3.41|P-value:3.20E-4||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML122C(YML122C_d)|FD-Score:5.08|P-value:1.85E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YMR247W-A(YMR247W-A_p)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function Gene:YNR048W(YNR048W)|FD-Score:-3.12|P-value:9.05E-4||SGD DESC:Protein that interacts with phospholipid translocase (flippase) Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication Gene:YNR071C(YNR071C_p)|FD-Score:3.11|P-value:9.21E-4||SGD DESC:Putative aldose 1-epimerase Gene:YOR105W(YOR105W_p)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YOR225W(YOR225W_d)|FD-Score:-3.41|P-value:3.23E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YSP2(YDR326C)|FD-Score:4.96|P-value:3.51E-7||SGD DESC:Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication Gene:ZRC1(YMR243C)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKL165C9.681.92E-224.64MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YJL031C5.032.40E-70.48BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YJL035C4.562.61E-60.05TAD2Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
YOR159C4.513.29E-60.15SME1Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
YOR335C4.366.62E-60.16ALA1Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
YJL090C4.191.39E-50.10DPB11DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
YEL034W4.092.17E-50.06HYP2Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
YER133W4.032.83E-50.03GLC7Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest
YBR155W3.993.27E-50.01CNS1TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
YJR112W3.993.35E-50.01NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YOR244W3.983.42E-50.06ESA1Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy
YER003C3.924.48E-50.05PMI40Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
YLR005W3.865.56E-50.01SSL1Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p
YOR206W3.855.86E-50.15NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YHR190W3.701.06E-40.08ERG9Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL020C18.704.90E-78RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YER090W13.704.23E-43TRP2Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
YKL211C12.303.62E-35TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YDR127W10.904.92E-28ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YDR504C8.509.85E-18SPG3Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
YDR354W8.451.44E-17TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YDR008C_d7.341.08E-13YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR087C6.932.10E-12CSF1Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YGL013C5.571.28E-8PDR1Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
YFR023W5.286.63E-8PES4Poly(A) binding protein, suppressor of DNA polymerase epsilon mutation; PES4 has a paralog, MIP6, that arose from the whole genome duplication
YML122C_d5.081.85E-7YML122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR071C5.012.68E-7TVP18Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
YOR120W5.012.69E-7GCY1Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
YDR326C4.963.51E-7YSP2Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
YBR039W4.943.97E-7ATP3Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis

GO enrichment analysis for SGTC_2312
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4052.85E-232SGTC_21976588183 93.1 μMChembridge (Fragment library)8841360.136986
0.3683.55E-189SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0326087
0.3052.04E-127SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.03125
0.3044.10E-126SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.103448plasma membrane duress
0.3004.25E-123SGTC_24725377652 49.4 μMMiscellaneous16109030.115942TRP & mitochondrial translation
0.2977.04E-121SGTC_22547938975 95.2 μMChembridge (Fragment library)29692770.1
0.2963.61E-120SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0985916
0.2961.21E-119SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0491803
0.2903.93E-115SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0952381
0.2901.33E-114SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.0921053
0.2901.68E-114SGTC_505gw-5074 63.9 μMICCB bioactive library59242080.0810811
0.2878.59E-113SGTC_2752diclazuril 33.1 μMMiscellaneous4563890.0886076
0.2794.96E-106SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)30151890.0151515
0.2781.39E-105SGTC_1699st031318 29.1 μMTimTec (Natural product derivative library)53406580.114286
0.2782.52E-105SGTC_18825615643 20.0 μMMiscellaneous22530750.121951TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3238913347049.47 μM0.2807023534688Chembridge (Drug-like library)275.365983.29613
SGTC_3255913907249.47 μM0.27419417646060Chembridge (Drug-like library)309.811043.48213
SGTC_2897904859812.99 μM0.2656256467404Chembridge (Drug-like library)283.321782.99113
SGTC_3051909377849.47 μM0.26470617217378Chembridge (Drug-like library)338.443343.5903
SGTC_2961908329032.55 μM0.26229517741490Chembridge (Drug-like library)295.784463.47513
SGTC_11574092-039427.6 μM0.260873659885ChemDiv (Drug-like library)358.861823.7703
SGTC_1456k015-001213 μM0.2459022989616ChemDiv (Drug-like library)363.149873.03802
SGTC_1686st019653115.5 μM0.24074197252TimTec (Natural product derivative library)173.211141.90401mitochondrial processes
SGTC_3049909222349.47 μM0.2388068623917Chembridge (Drug-like library)328.835843.93502
SGTC_13853091-483414.1 μM0.2380954072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion