5986382

2-(1-anilinoethylidene)cyclohexane-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2317
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 877883
SMILES CC(=C1C(=O)CCCC1=O)NC2=CC=CC=C2
Standardized SMILES CC(=Nc1ccccc1)C2=C(O)CCCC2=O
Molecular weight 229.2744
ALogP 2.08
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.23
% growth inhibition (Hom. pool) 5.41


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 877883
Download HIP data (tab-delimited text)  (excel)
Gene:ATM1(YMR301C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.09||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:HEM3(YDL205C)|FD-Score:-3.74|P-value:9.07E-5|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HRT1(YOL133W)|FD-Score:3.82|P-value:6.60E-5|Clearance:0.14||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MES1(YGR264C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.09||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MYO1(YHR023W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NOB1(YOR056C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.07||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PRE7(YBL041W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.14||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RCL1(YOL010W)|FD-Score:4.3|P-value:8.54E-6|Clearance:0.32||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RPC17(YJL011C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.16||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RVB2(YPL235W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.02||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC8(YPR055W)|FD-Score:-4.63|P-value:1.85E-6|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TRE2(YOR256C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.06||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:ULP2(YIL031W)|FD-Score:4.39|P-value:5.54E-6|Clearance:0.32||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YBR190W(YBR190W_d)|FD-Score:-3.21|P-value:6.73E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W Gene:ATM1(YMR301C)|FD-Score:3.5|P-value:2.35E-4|Clearance:0.09||SGD DESC:Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol Gene:HEM3(YDL205C)|FD-Score:-3.74|P-value:9.07E-5|Clearance:0||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HRT1(YOL133W)|FD-Score:3.82|P-value:6.60E-5|Clearance:0.14||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:MES1(YGR264C)|FD-Score:3.59|P-value:1.65E-4|Clearance:0.09||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MYO1(YHR023W)|FD-Score:-3.71|P-value:1.02E-4|Clearance:0||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NOB1(YOR056C)|FD-Score:3.66|P-value:1.26E-4|Clearance:0.07||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:NSP1(YJL041W)|FD-Score:3.35|P-value:3.98E-4|Clearance:0.2||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress Gene:PRE7(YBL041W)|FD-Score:3.15|P-value:8.16E-4|Clearance:0.14||SGD DESC:Beta 6 subunit of the 20S proteasome Gene:RCL1(YOL010W)|FD-Score:4.3|P-value:8.54E-6|Clearance:0.32||SGD DESC:Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected Gene:RPC17(YJL011C)|FD-Score:-3.11|P-value:9.45E-4|Clearance:0||SGD DESC:RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress Gene:RPO31(YOR116C)|FD-Score:3.98|P-value:3.48E-5|Clearance:0.16||SGD DESC:RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21 Gene:RVB2(YPL235W)|FD-Score:3.68|P-value:1.15E-4|Clearance:0.02||SGD DESC:ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly Gene:SEC8(YPR055W)|FD-Score:-4.63|P-value:1.85E-6|Clearance:0||SGD DESC:Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress Gene:TRE2(YOR256C)|FD-Score:3.41|P-value:3.26E-4|Clearance:0.06||SGD DESC:Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication Gene:ULP2(YIL031W)|FD-Score:4.39|P-value:5.54E-6|Clearance:0.32||SGD DESC:Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate Gene:YBR190W(YBR190W_d)|FD-Score:-3.21|P-value:6.73E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 877883
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ADY3(YDL239C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:APA1(YCL050C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ARG7(YMR062C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ASF1(YJL115W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG10(YLL042C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BCK1(YJL095W)|FD-Score:-4.8|P-value:7.88E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCR4(YAL021C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COA1(YIL157C)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:DAL5(YJR152W)|FD-Score:4.27|P-value:9.85E-6||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DCS1(YLR270W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIN7(YDR263C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:FDC1(YDR539W)|FD-Score:-3.78|P-value:7.80E-5||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FRE7(YOL152W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GIS2(YNL255C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HMI1(YOL095C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IFM1(YOL023W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IME4(YGL192W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ITC1(YGL133W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KRE2(YDR483W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LIA1(YJR070C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS2(YGR235C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRPL10(YNL284C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NAM2(YLR382C)|FD-Score:-4.28|P-value:9.25E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NAM8(YHR086W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:OSH3(YHR073W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCI8(YIL071C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEX17(YNL214W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHM8(YER037W)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRM4(YPL156C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RAD1(YPL022W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD16(YBR114W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RBG1(YAL036C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPL17B(YJL177W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:SRL1(YOR247W)|FD-Score:6.77|P-value:6.42E-12||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:STE13(YOR219C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:UBC5(YDR059C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:WTM1(YOR230W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR027C(YBR027C_d)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR090C(YCR090C_p)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR199W(YDR199W_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YER188W(YER188W_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YJR003C(YJR003C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR122C(YLR122C_d)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR455W(YLR455W_p)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML020W(YML020W_p)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Putative protein of unknown function Gene:YML037C(YML037C_p)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNR040W(YNR040W_p)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL099C(YOL099C_d)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOR024W(YOR024W_d)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR059C(YPR059C_d)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR123C(YPR123C_d)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS5(YGL259W_p)|FD-Score:-3.75|P-value:8.68E-5||SGD DESC:Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p Gene:YTA6(YPL074W)|FD-Score:-4.92|P-value:4.43E-7||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:ABP140(YOR239W)|FD-Score:3.1|P-value:9.84E-4||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ADY3(YDL239C)|FD-Score:3.64|P-value:1.34E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:APA1(YCL050C)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication Gene:ARG7(YMR062C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine Gene:ASF1(YJL115W)|FD-Score:-3.8|P-value:7.27E-5||SGD DESC:Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition Gene:ATG10(YLL042C)|FD-Score:3.78|P-value:7.94E-5||SGD DESC:Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy Gene:BCK1(YJL095W)|FD-Score:-4.8|P-value:7.88E-7||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:CCR4(YAL021C)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:COA1(YIL157C)|FD-Score:-4.06|P-value:2.42E-5||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:DAL5(YJR152W)|FD-Score:4.27|P-value:9.85E-6||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DCS1(YLR270W)|FD-Score:-3.67|P-value:1.24E-4||SGD DESC:Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress Gene:DGR2(YKL121W_p)|FD-Score:4.45|P-value:4.28E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIN7(YDR263C)|FD-Score:3.13|P-value:8.76E-4||SGD DESC:Mitochondrial nuclease functioning in DNA repair and replication, modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination Gene:FDC1(YDR539W)|FD-Score:-3.78|P-value:7.80E-5||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FRE7(YOL152W)|FD-Score:3.15|P-value:8.06E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GIS2(YNL255C)|FD-Score:3.12|P-value:9.00E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:HMI1(YOL095C)|FD-Score:-3.16|P-value:7.89E-4||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:IFM1(YOL023W)|FD-Score:3.75|P-value:8.92E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IME4(YGL192W)|FD-Score:4.01|P-value:3.03E-5||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:ITC1(YGL133W)|FD-Score:-4.13|P-value:1.83E-5||SGD DESC:Subunit of the ATP-dependent Isw2p-Itc1p chromatin remodeling complex, required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex Gene:KRE2(YDR483W)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication Gene:LCB4(YOR171C)|FD-Score:-3.87|P-value:5.45E-5||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LIA1(YJR070C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MNN2(YBR015C)|FD-Score:3.13|P-value:8.82E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOS2(YGR235C)|FD-Score:3.72|P-value:9.79E-5||SGD DESC:Mitochondrial inner membrane protein; non-essential component of the mitochondrial inner membrane organizing system (MINOS, MitOS, or MICOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture Gene:MRPL10(YNL284C)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels Gene:NAM2(YLR382C)|FD-Score:-4.28|P-value:9.25E-6||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:NAM8(YHR086W)|FD-Score:-3.44|P-value:2.95E-4||SGD DESC:RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function Gene:OSH3(YHR073W)|FD-Score:-3.49|P-value:2.40E-4||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:PCI8(YIL071C)|FD-Score:4.58|P-value:2.36E-6||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEX17(YNL214W)|FD-Score:-3.87|P-value:5.34E-5||SGD DESC:Peroxisomal membrane peroxin and subunit of the docking complex that facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis Gene:PHM8(YER037W)|FD-Score:4.67|P-value:1.54E-6||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PRM4(YPL156C)|FD-Score:-3.55|P-value:1.89E-4||SGD DESC:Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift Gene:RAD1(YPL022W)|FD-Score:4.33|P-value:7.49E-6||SGD DESC:Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein Gene:RAD16(YBR114W)|FD-Score:3.19|P-value:7.23E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RBG1(YAL036C)|FD-Score:3.19|P-value:7.17E-4||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RPL17B(YJL177W)|FD-Score:-3.49|P-value:2.45E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:SRL1(YOR247W)|FD-Score:6.77|P-value:6.42E-12||SGD DESC:Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication Gene:STE13(YOR219C)|FD-Score:3.53|P-value:2.05E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:UBC5(YDR059C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication Gene:WTM1(YOR230W)|FD-Score:-3.11|P-value:9.29E-4||SGD DESC:Transcriptional modulator involved in regulation of meiosis, silencing, and expression of RNR genes; required for nuclear localization of the ribonucleotide reductase small subunit Rnr2p and Rnr4p; contains WD repeats Gene:YBR027C(YBR027C_d)|FD-Score:4.99|P-value:3.08E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YCL001W-A(YCL001W-A_p)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YCR090C(YCR090C_p)|FD-Score:-3.23|P-value:6.22E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene Gene:YDR199W(YDR199W_d)|FD-Score:3.44|P-value:2.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity Gene:YER188W(YER188W_d)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YJR003C(YJR003C_p)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis Gene:YKL033W-A(YKL033W-A_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; similar to uncharacterized proteins from other fungi Gene:YLR122C(YLR122C_d)|FD-Score:4.09|P-value:2.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR455W(YLR455W_p)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); protein abundance increases in response to DNA replication stress Gene:YML020W(YML020W_p)|FD-Score:4.34|P-value:7.23E-6||SGD DESC:Putative protein of unknown function Gene:YML037C(YML037C_p)|FD-Score:4.18|P-value:1.44E-5||SGD DESC:Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene Gene:YNR040W(YNR040W_p)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOL099C(YOL099C_d)|FD-Score:3.89|P-value:5.09E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes Gene:YOR024W(YOR024W_d)|FD-Score:4.69|P-value:1.39E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPR059C(YPR059C_d)|FD-Score:4.78|P-value:8.73E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W Gene:YPR123C(YPR123C_d)|FD-Score:3.16|P-value:8.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPS5(YGL259W_p)|FD-Score:-3.75|P-value:8.68E-5||SGD DESC:Protein with similarity to GPI-anchored aspartic proteases such as Yap1p and Yap3p Gene:YTA6(YPL074W)|FD-Score:-4.92|P-value:4.43E-7||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YIL031W4.395.54E-60.32ULP2Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins, plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate
YOL010W4.308.54E-60.32RCL1Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
YOR116C3.983.48E-50.15RPO31RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
YOL133W3.826.60E-50.14HRT1RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF
YPL235W3.681.15E-40.02RVB2ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
YOR056C3.661.26E-40.07NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YGR264C3.591.65E-40.09MES1Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
YMR301C3.502.35E-40.09ATM1Mitochondrial inner membrane ATP-binding cassette (ABC) transporter, exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol
YOR256C3.413.26E-40.06TRE2Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
YJL041W3.353.98E-40.20NSP1FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier; found in stable complex with Nup82p, Gle2p and two other FG-nucleoporins (Nup159p and Nup116p); also found in stable complex with Nic96p and two other FG-nucleoproteins (Nup49p and Nup57p); forms foci at the nuclear periphery upon DNA replication stress
YBL041W3.158.16E-40.14PRE7Beta 6 subunit of the 20S proteasome
YDL016C_d3.010.001320.00YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YDR118W3.000.001330.23APC4Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
YLR116W2.770.002790.07MSL5Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA
YNL039W2.700.003470.08BDP1Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR247W6.776.42E-12SRL1Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
YBR027C_d4.993.08E-7YBR027C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR059C_d4.788.73E-7YPR059C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
YOR024W_d4.691.39E-6YOR024W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YER037W4.671.54E-6PHM8Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p
YIL071C4.582.36E-6PCI8Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
YKL121W_p4.454.28E-6DGR2_pProtein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds
YML020W_p4.347.23E-6YML020W_pPutative protein of unknown function
YPL022W4.337.49E-6RAD1Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
YJR152W4.279.85E-6DAL5Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
YML037C_p4.181.44E-5YML037C_pPutative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
YLR122C_d4.092.16E-5YLR122C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C
YGL192W4.013.03E-5IME4Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids
YDR483W3.963.72E-5KRE2Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
YOL099C_d3.895.09E-5YOL099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes

GO enrichment analysis for SGTC_2317
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0863.94E-11SGTC_1930st074634 34.3 μMTimTec (Natural product derivative library)6842530.150943RNA pol III & RNase P/MRP
0.0806.03E-10SGTC_23286201408 200.0 μMChembridge (Fragment library)28904180.136364
0.0741.21E-8SGTC_13371486-1011 40.3 μMChemDiv (Drug-like library)X13370.220339
0.0706.64E-8SGTC_11493909-7961 23.3 μMChemDiv (Drug-like library)7352610.156863
0.0655.02E-7SGTC_12620737-0114 49.6 μMChemDiv (Drug-like library)7659190.0895522
0.0655.14E-7SGTC_2738aclarubicin 5.5 μMMiscellaneous4514150.0917431RNA pol III & RNase P/MRP
0.0631.28E-6SGTC_6781218-2179 85.9 μMChemDiv (Drug-like library)68200810.114754RNA pol III & RNase P/MRP
0.0621.81E-6SGTC_32209131269 49.5 μMChembridge (Drug-like library)177222060.193548
0.0622.05E-6SGTC_10154130-4525 82.0 μMChemDiv (Drug-like library)13632370.25454560S ribosome export
0.0612.56E-6SGTC_23266164455 177.6 μMChembridge (Fragment library)28887900.181818mitochondrial processes
0.0587.43E-6SGTC_1130099-0221 2.5 μMChemDiv (Drug-like library)892190.09375
0.0571.21E-5SGTC_21715739187 198.1 μMChembridge (Fragment library)22627610.142857copper-dependent oxidative stress
0.0571.24E-5SGTC_24845809818 12.0 μMMiscellaneous28705480.153846
0.0552.04E-5SGTC_2544sphondin 68.5 μMMicrosource (Natural product library)1081040.105263RNA pol III & RNase P/MRP
0.0542.81E-5SGTC_6120302-0167 22.4 μMChemDiv (Drug-like library)2358210.14copper-dependent oxidative stress

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20645220758200 μM0.354167788518Chembridge (Fragment library)263.312221.92615
SGTC_12780874-0585172 μM0.333333766306ChemDiv (Drug-like library)234.360424.3721
SGTC_13621611-0397101 μM0.33333327816ChemDiv (Drug-like library)227.258523.65612
SGTC_21215257820149.64 μM0.325791256Chembridge (Fragment library)204.225180.85313
SGTC_8860866-0150153 μM0.318182271463ChemDiv (Drug-like library)199.248423.14201
SGTC_14334060-00237.6 μM0.3137263840627ChemDiv (Drug-like library)274.29841.01924tubulin folding & SWR complex
SGTC_21806301957200 μM0.3125845462Chembridge (Fragment library)249.285642.09415RSC complex & mRNA processing
SGTC_3232913287449.47 μM0.3111113784940Chembridge (Drug-like library)255.268622.4713RPP1 & pyrimidine depletion
SGTC_2887904478458.44 μM0.36465546Chembridge (Drug-like library)323.4322.98313fatty acid desaturase (OLE1)
SGTC_3203911354349.47 μM0.29787217018012Chembridge (Drug-like library)241.28513.08412