7373003

(5Z)-5-[(3-methoxyanilino)methylidene]-1-methyl-2-sulfanylidene-1,3-diazinane-4,6-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2320
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 5415828
SMILES CN1C(=O)C(=CNC2=CC(=CC=C2)OC)C(=O)NC1=S
Standardized SMILES COc1cccc(NC=C2C(=O)NC(=S)N(C)C2=O)c1
Molecular weight 291.3256
ALogP 1.29
H-bond donor count 2
H-bond acceptor count 5
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 1.72
% growth inhibition (Hom. pool) 3.18


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5415828
Download HIP data (tab-delimited text)  (excel)
Gene:CCT7(YJL111W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC60(YPL160W)|FD-Score:4.72|P-value:1.15E-6|Clearance:0.29||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:MED4(YOR174W)|FD-Score:3.57|P-value:1.82E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MED6(YHR058C)|FD-Score:-3.79|P-value:7.45E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MED8(YBR193C)|FD-Score:-3.78|P-value:7.85E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOB1(YIL106W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.07||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR10(YOR160W)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.19||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP19(YGR251W)|FD-Score:-4|P-value:3.23E-5|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NOP4(YPL043W)|FD-Score:-3.82|P-value:6.58E-5|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PDS5(YMR076C)|FD-Score:-3.24|P-value:5.90E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POP4(YBR257W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP24(YMR268C)|FD-Score:5.49|P-value:2.06E-8|Clearance:0.49||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP43(YGL120C)|FD-Score:-4.64|P-value:1.74E-6|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:PUP2(YGR253C)|FD-Score:-4.3|P-value:8.59E-6|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RAD53(YPL153C)|FD-Score:-4.96|P-value:3.58E-7|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.17||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL18A(YOL120C)|FD-Score:5.24|P-value:8.05E-8|Clearance:0.49||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPT3(YDR394W)|FD-Score:3.79|P-value:7.41E-5|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:5.93|P-value:1.47E-9|Clearance:0.49||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC6(YCR052W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC17(YBL050W)|FD-Score:4.38|P-value:5.90E-6|Clearance:0.32||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SEC26(YDR238C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.04||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SIS1(YNL007C)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.06||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:SME1(YOR159C)|FD-Score:-3.15|P-value:8.19E-4|Clearance:0||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:STH1(YIL126W)|FD-Score:4.75|P-value:1.02E-6|Clearance:0.03||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFB2(YPL122C)|FD-Score:4.44|P-value:4.60E-6|Clearance:0.05||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TIM10(YHR005C-A)|FD-Score:3.88|P-value:5.27E-5|Clearance:0.01||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM54(YJL054W)|FD-Score:-3.18|P-value:7.42E-4|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane Gene:CCT7(YJL111W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.13||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CDC60(YPL160W)|FD-Score:4.72|P-value:1.15E-6|Clearance:0.29||SGD DESC:Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA Gene:MED4(YOR174W)|FD-Score:3.57|P-value:1.82E-4|Clearance:0.07||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MED6(YHR058C)|FD-Score:-3.79|P-value:7.45E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress Gene:MED8(YBR193C)|FD-Score:-3.78|P-value:7.85E-5|Clearance:0||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MOB1(YIL106W)|FD-Score:3.87|P-value:5.55E-5|Clearance:0.07||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:MTR10(YOR160W)|FD-Score:4.06|P-value:2.40E-5|Clearance:0.19||SGD DESC:Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOP19(YGR251W)|FD-Score:-4|P-value:3.23E-5|Clearance:0||SGD DESC:Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes Gene:NOP4(YPL043W)|FD-Score:-3.82|P-value:6.58E-5|Clearance:0||SGD DESC:Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs) Gene:PDS5(YMR076C)|FD-Score:-3.24|P-value:5.90E-4|Clearance:0||SGD DESC:Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis Gene:POP4(YBR257W)|FD-Score:3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP24(YMR268C)|FD-Score:5.49|P-value:2.06E-8|Clearance:0.49||SGD DESC:Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling Gene:PRP43(YGL120C)|FD-Score:-4.64|P-value:1.74E-6|Clearance:0||SGD DESC:RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome Gene:PUP2(YGR253C)|FD-Score:-4.3|P-value:8.59E-6|Clearance:0||SGD DESC:Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Gene:RAD53(YPL153C)|FD-Score:-4.96|P-value:3.58E-7|Clearance:0||SGD DESC:Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication Gene:RHO1(YPR165W)|FD-Score:3.23|P-value:6.25E-4|Clearance:0.17||SGD DESC:GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p) Gene:RPL18A(YOL120C)|FD-Score:5.24|P-value:8.05E-8|Clearance:0.49||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPN6(YDL097C)|FD-Score:-3.2|P-value:6.86E-4|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPT3(YDR394W)|FD-Score:3.79|P-value:7.41E-5|Clearance:0.23||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:5.93|P-value:1.47E-9|Clearance:0.49||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RSC6(YCR052W)|FD-Score:3.49|P-value:2.40E-4|Clearance:0.03||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:SEC17(YBL050W)|FD-Score:4.38|P-value:5.90E-6|Clearance:0.32||SGD DESC:Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP Gene:SEC26(YDR238C)|FD-Score:3.4|P-value:3.40E-4|Clearance:0.04||SGD DESC:Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP) Gene:SIS1(YNL007C)|FD-Score:3.46|P-value:2.74E-4|Clearance:0.06||SGD DESC:Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress Gene:SME1(YOR159C)|FD-Score:-3.15|P-value:8.19E-4|Clearance:0||SGD DESC:Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E Gene:STH1(YIL126W)|FD-Score:4.75|P-value:1.02E-6|Clearance:0.03||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TFB2(YPL122C)|FD-Score:4.44|P-value:4.60E-6|Clearance:0.05||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:TIM10(YHR005C-A)|FD-Score:3.88|P-value:5.27E-5|Clearance:0.01||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TIM54(YJL054W)|FD-Score:-3.18|P-value:7.42E-4|Clearance:0||SGD DESC:Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5415828
Download HOP data (tab-delimited text)  (excel)
Gene:ADH2(YMR303C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AFG3(YER017C)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APN2(YBL019W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII Gene:ARF2(YDL137W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:BEM1(YBR200W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BLS1(YLR408C_p)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNA4(YBL098W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CAP2(YIL034C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COQ3(YOL096C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:COX10(YPL172C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CUE2(YKL090W)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination Gene:CUS2(YNL286W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DAS2(YDR020C_p)|FD-Score:6.22|P-value:2.45E-10||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DRS2(YAL026C)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSE3(YOR264W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EFT1(YOR133W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ERV2(YPR037C)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:ESBP6(YNL125C)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FUS2(YMR232W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAS1(YMR307W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GIN4(YDR507C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:-3.73|P-value:9.51E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLR1(YPL091W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GUF1(YLR289W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HAA1(YPR008W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HBT1(YDL223C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IAH1(YOR126C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:INO4(YOL108C)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LHS1(YKL073W)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOH1(YJL038C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MCM21(YDR318W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM30(YLR368W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MHF2(YDL160C-A)|FD-Score:5.47|P-value:2.22E-8||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MPM1(YJL066C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRP51(YPL118W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MUM3(YOR298W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NAB6(YML117W)|FD-Score:-6.57|P-value:2.51E-11||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NUM1(YDR150W)|FD-Score:4.33|P-value:7.46E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PCK1(YKR097W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL6(YER059W)|FD-Score:-4.49|P-value:3.63E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PEA2(YER149C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHO86(YJL117W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PLB3(YOL011W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PNP1(YLR209C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPM1(YDR435C)|FD-Score:-4.54|P-value:2.77E-6||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RAD4(YER162C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:REV3(YPL167C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPL21A(YBR191W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RSA3(YLR221C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RXT2(YBR095C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SET5(YHR207C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFM1(YOR021C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SNX3(YOR357C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOH1(YGL127C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSF1(YHR066W)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SXM1(YDR395W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYT1(YPR095C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TDA3(YHR009C_p)|FD-Score:6.01|P-value:9.24E-10||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIP41(YPR040W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOS2(YGR221C)|FD-Score:-7.27|P-value:1.85E-13||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRK1(YJL129C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TSA1(YML028W)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TVP38(YKR088C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UPS3(YDR185C)|FD-Score:6.88|P-value:2.90E-12||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YAP1801(YHR161C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR134W(YBR134W_d)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR007C(YCR007C_p)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YDR535C(YDR535C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL045C(YEL045C_d)|FD-Score:4.29|P-value:8.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Putative protein of unknown function Gene:YGR035C(YGR035C_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YIL060W(YIL060W_p)|FD-Score:4.4|P-value:5.52E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL163C(YIL163C_p)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL106C-A(YKL106C-A_p)|FD-Score:-4.38|P-value:5.89E-6||SGD DESC:Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi Gene:YLR211C(YLR211C_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR352W(YLR352W_p)|FD-Score:-5.04|P-value:2.28E-7||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:-3.88|P-value:5.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL303W(YNL303W_d)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL036W(YOL036W_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL062W(YPL062W_d)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPT53(YNL093W)|FD-Score:8.18|P-value:1.37E-16||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication Gene:ADH2(YMR303C)|FD-Score:4.46|P-value:4.03E-6||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AFG3(YER017C)|FD-Score:-3.25|P-value:5.67E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:AFT1(YGL071W)|FD-Score:-4.02|P-value:2.97E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:APN2(YBL019W)|FD-Score:-3.84|P-value:6.16E-5||SGD DESC:Class II abasic (AP) endonuclease involved in repair of DNA damage; homolog of human HAP1 and E. coli exoIII Gene:ARF2(YDL137W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication Gene:BEM1(YBR200W)|FD-Score:-4.34|P-value:7.12E-6||SGD DESC:Protein containing SH3-domains, involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p Gene:BLS1(YLR408C_p)|FD-Score:-3.67|P-value:1.23E-4||SGD DESC:Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene Gene:BNA4(YBL098W)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Kynurenine 3-mono oxygenase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; putative therapeutic target for Huntington disease Gene:CAP2(YIL034C)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:COQ3(YOL096C)|FD-Score:-3.1|P-value:9.69E-4||SGD DESC:O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein Gene:COX10(YPL172C)|FD-Score:4.64|P-value:1.77E-6||SGD DESC:Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders Gene:CUE2(YKL090W)|FD-Score:-3.28|P-value:5.18E-4||SGD DESC:Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination Gene:CUS2(YNL286W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Protein that binds to U2 snRNA and Prp11p, may be involved in U2 snRNA folding; contains two RNA recognition motifs (RRMs) Gene:DAS2(YDR020C_p)|FD-Score:6.22|P-value:2.45E-10||SGD DESC:Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases Gene:DRS2(YAL026C)|FD-Score:3.75|P-value:8.68E-5||SGD DESC:Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease Gene:DSE3(YOR264W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EFT1(YOR133W)|FD-Score:-3.22|P-value:6.36E-4||SGD DESC:Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication Gene:ERV2(YPR037C)|FD-Score:-4.03|P-value:2.77E-5||SGD DESC:Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER Gene:ESBP6(YNL125C)|FD-Score:4.64|P-value:1.78E-6||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FUS2(YMR232W)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Cytoplasmic protein localized to the shmoo tip; required for the alignment of parental nuclei before nuclear fusion during mating Gene:GAS1(YMR307W)|FD-Score:3.21|P-value:6.72E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GIN4(YDR507C)|FD-Score:3.14|P-value:8.49E-4||SGD DESC:Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication Gene:GIS2(YNL255C)|FD-Score:-3.73|P-value:9.51E-5||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GLR1(YPL091W)|FD-Score:4.07|P-value:2.38E-5||SGD DESC:Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress Gene:GUF1(YLR289W)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor Gene:HAA1(YPR008W)|FD-Score:-3.7|P-value:1.09E-4||SGD DESC:Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress Gene:HBT1(YDL223C)|FD-Score:3.09|P-value:9.94E-4||SGD DESC:Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis Gene:IAH1(YOR126C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Isoamyl acetate-hydrolyzing esterase, required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing Gene:INO4(YOL108C)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain Gene:LHS1(YKL073W)|FD-Score:4.45|P-value:4.38E-6||SGD DESC:Molecular chaperone of the endoplasmic reticulum lumen, involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway Gene:LOH1(YJL038C)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1 Gene:MCM21(YDR318W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MDM30(YLR368W)|FD-Score:-3.77|P-value:8.05E-5||SGD DESC:F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains Gene:MHF2(YDL160C-A)|FD-Score:5.47|P-value:2.22E-8||SGD DESC:Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2 Gene:MPM1(YJL066C)|FD-Score:3.12|P-value:9.15E-4||SGD DESC:Mitochondrial intermembrane space protein of unknown function Gene:MRP51(YPL118W)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MUM3(YOR298W)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein of unknown function involved in the organization of the outer spore wall layers; has similarity to the tafazzins superfamily of acyltransferases Gene:NAB6(YML117W)|FD-Score:-6.57|P-value:2.51E-11||SGD DESC:Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP Gene:NUM1(YDR150W)|FD-Score:4.33|P-value:7.46E-6||SGD DESC:Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex Gene:OMS1(YDR316W)|FD-Score:-3.36|P-value:3.83E-4||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PCK1(YKR097W)|FD-Score:3.3|P-value:4.77E-4||SGD DESC:Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol Gene:PCL6(YER059W)|FD-Score:-4.49|P-value:3.63E-6||SGD DESC:Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding Gene:PEA2(YER149C)|FD-Score:3.12|P-value:9.19E-4||SGD DESC:Coiled-coil polarisome protein required for polarized morphogenesis, cell fusion, and low affinity Ca2+ influx; forms polarisome complex with Bni1p, Bud6p, and Spa2p; localizes to sites of polarized growth Gene:PHO86(YJL117W)|FD-Score:3.65|P-value:1.31E-4||SGD DESC:Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress Gene:PLB3(YOL011W)|FD-Score:3.23|P-value:6.13E-4||SGD DESC:Phospholipase B (lysophospholipase) involved in phospholipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro Gene:PNP1(YLR209C)|FD-Score:3.86|P-value:5.64E-5||SGD DESC:Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway Gene:PPM1(YDR435C)|FD-Score:-4.54|P-value:2.77E-6||SGD DESC:Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits Gene:RAD4(YER162C)|FD-Score:-3.17|P-value:7.66E-4||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:REV3(YPL167C)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair Gene:RPL21A(YBR191W)|FD-Score:3.48|P-value:2.52E-4||SGD DESC:Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication Gene:RPS10B(YMR230W)|FD-Score:4.68|P-value:1.46E-6||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RSA3(YLR221C)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RXT2(YBR095C)|FD-Score:3.75|P-value:8.97E-5||SGD DESC:Subunit of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth Gene:SAC1(YKL212W)|FD-Score:5.13|P-value:1.42E-7||SGD DESC:Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism Gene:SET5(YHR207C)|FD-Score:4.58|P-value:2.29E-6||SGD DESC:Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus Gene:SFM1(YOR021C)|FD-Score:-4.05|P-value:2.57E-5||SGD DESC:SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism Gene:SNX3(YOR357C)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p Gene:SOH1(YGL127C)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits Gene:SSF1(YHR066W)|FD-Score:3.75|P-value:8.70E-5||SGD DESC:Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication Gene:SSO1(YPL232W)|FD-Score:4.3|P-value:8.49E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:SXM1(YDR395W)|FD-Score:-3.2|P-value:6.89E-4||SGD DESC:Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1 Gene:SYT1(YPR095C)|FD-Score:-3.37|P-value:3.82E-4||SGD DESC:Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain Gene:TDA3(YHR009C_p)|FD-Score:6.01|P-value:9.24E-10||SGD DESC:Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1 Gene:TIP41(YPR040W)|FD-Score:3.37|P-value:3.82E-4||SGD DESC:Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress Gene:TOS2(YGR221C)|FD-Score:-7.27|P-value:1.85E-13||SGD DESC:Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p Gene:TRK1(YJL129C)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication Gene:TSA1(YML028W)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Thioredoxin peroxidase; acts as both a ribosome-associated and free cytoplasmic antioxidant; self-associates to form a high-molecular weight chaperone complex under oxidative stress; deletion results in mutator phenotype; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:TVP38(YKR088C)|FD-Score:3.34|P-value:4.19E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern Gene:UPS3(YDR185C)|FD-Score:6.88|P-value:2.90E-12||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:YAP1801(YHR161C)|FD-Score:3.2|P-value:6.96E-4||SGD DESC:Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication Gene:YBR064W(YBR064W_d)|FD-Score:-3.91|P-value:4.53E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the uncharacterized ORF YBR063C Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR134W(YBR134W_d)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YCR007C(YCR007C_p)|FD-Score:-3.54|P-value:1.96E-4||SGD DESC:Putative integral membrane protein, member of DUP240 gene family; YCR007C is not an essential gene Gene:YDR524C-B(YDR524C-B_p)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative protein of unknown function; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication Gene:YDR535C(YDR535C_d)|FD-Score:3.33|P-value:4.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEL045C(YEL045C_d)|FD-Score:4.29|P-value:8.80E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YGL188C-A(YGL188C-A_p)|FD-Score:4.62|P-value:1.96E-6||SGD DESC:Putative protein of unknown function Gene:YGR035C(YGR035C_p)|FD-Score:-3.71|P-value:1.04E-4||SGD DESC:Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance Gene:YIL060W(YIL060W_p)|FD-Score:4.4|P-value:5.52E-6||SGD DESC:Putative protein of unknown function; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene Gene:YIL163C(YIL163C_p)|FD-Score:6.24|P-value:2.17E-10||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YKL106C-A(YKL106C-A_p)|FD-Score:-4.38|P-value:5.89E-6||SGD DESC:Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi Gene:YLR211C(YLR211C_p)|FD-Score:-5.16|P-value:1.21E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR211C is not an essential gene; ORF contains an intron Gene:YLR352W(YLR352W_p)|FD-Score:-5.04|P-value:2.28E-7||SGD DESC:Putative protein of unknown function with similarity to F-box proteins; interacts with Skp1p and Cdc53p; YLR352W is not an essential gene Gene:YMR158W-B(YMR158W-B_d)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C Gene:YMR242W-A(YMR242W-A_p)|FD-Score:4.14|P-value:1.74E-5||SGD DESC:Putative protein of unknown function Gene:YNL120C(YNL120C_d)|FD-Score:-3.88|P-value:5.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene Gene:YNL303W(YNL303W_d)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOL036W(YOL036W_p)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YOR072W(YOR072W_d)|FD-Score:3.8|P-value:7.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YPL062W(YPL062W_d)|FD-Score:3.97|P-value:3.60E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation Gene:YPT53(YNL093W)|FD-Score:8.18|P-value:1.37E-16||SGD DESC:Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YOR117W5.931.47E-90.49RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YMR268C5.492.06E-80.49PRP24Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling
YOL120C5.248.05E-80.49RPL18ARibosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
YIL126W4.751.02E-60.03STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YPL160W4.721.15E-60.29CDC60Cytosolic leucyl tRNA synthetase, ligates leucine to the appropriate tRNA
YPL122C4.444.60E-60.05TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YBL050W4.385.90E-60.32SEC17Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; stimulates the ATPase activity of Sec18p; has similarity to mammalian alpha-SNAP
YOR160W4.062.40E-50.19MTR10Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YHR005C-A3.885.27E-50.01TIM10Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
YIL106W3.875.55E-50.07MOB1Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
YDR394W3.797.41E-50.23RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YOR174W3.571.82E-40.07MED4Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
YCR052W3.492.40E-40.04RSC6Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p
YNL007C3.462.74E-40.06SIS1Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
YDR238C3.403.40E-40.04SEC26Essential beta-coat protein of the COPI coatomer, involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL093W8.181.37E-16YPT53Rab family GTPase; similar to Ypt51p and Ypt52p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
YDR185C6.882.90E-12UPS3Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations
YIL163C_p6.242.17E-10YIL163C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YDR020C_p6.222.45E-10DAS2_pPutative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
YHR009C_p6.019.24E-10TDA3_pPutative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
YDL160C-A5.472.22E-8MHF2Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2
YKL212W5.131.42E-7SAC1Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
YMR230W4.681.46E-6RPS10BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication
YPL172C4.641.77E-6COX10Heme A:farnesyltransferase, catalyzes the first step in the conversion of protoheme to the heme A prosthetic group required for cytochrome c oxidase activity; human ortholog is associated with mitochondrial disorders
YNL125C4.641.78E-6ESBP6Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
YGL188C-A_p4.621.96E-6YGL188C-A_pPutative protein of unknown function
YHR207C4.582.29E-6SET5Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
YGL127C4.523.11E-6SOH1Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
YNL303W_d4.473.97E-6YNL303W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR303C4.464.03E-6ADH2Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1

GO enrichment analysis for SGTC_2320
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0881.66E-11SGTC_22287354201 200.0 μMChembridge (Fragment library)7534510.0694444endomembrane recycling
0.0822.82E-10SGTC_2236dapsone 200.0 μMMiscellaneous29550.0727273
0.0707.75E-8SGTC_30179080681 49.5 μMChembridge (Drug-like library)164519140.0759494
0.0631.09E-6SGTC_1836st055642 81.0 μMTimTec (Natural product derivative library)85710.0862069
0.0612.40E-6SGTC_32529135871 49.5 μMChembridge (Drug-like library)176828380.15493
0.0604.17E-6SGTC_23246149169 200.0 μMChembridge (Fragment library)28882310.125
0.0595.45E-6SGTC_1874staurosporine 7.6 μMICCB bioactive library468641930.129032cell wall signaling
0.0595.55E-6SGTC_22587960471 200.0 μMChembridge (Fragment library)29747080.0675676
0.0587.88E-6SGTC_2129girgensonine 121.1 μMChembridge (Fragment library)4426380.0597015heme biosynthesis & mitochondrial translocase
0.0526.05E-5SGTC_21405586273 200.0 μMChembridge (Fragment library)8544210.129032RSC complex & mRNA processing
0.0526.05E-5SGTC_24745321570 43.3 μMMiscellaneous28413840.114286
0.0526.78E-5SGTC_21245246320 200.0 μMChembridge (Fragment library)5793420.107692heme biosynthesis & mitochondrial translocase
0.0526.84E-5SGTC_23186029776 164.0 μMChembridge (Fragment library)8183250.104478
0.0517.87E-5SGTC_231b-estradiol 524.0 μMMiscellaneous4500.0641026
0.0519.79E-5SGTC_28317999036 19.5 μMChembridge (Drug-like library)29882700.148649

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2305744352269.23 μM0.65415886Chembridge (Fragment library)279.2901031.51625
SGTC_7263448-439134.2 μM0.3235291356692ChemDiv (Drug-like library)388.439044.43814
SGTC_2900532852885.29 μM0.322034795359Chembridge (Drug-like library)261.703583.26212Golgi
SGTC_3204911418349.47 μM0.31666717027248Chembridge (Drug-like library)329.2463533.40317
SGTC_2827799384771.43 μM0.31252985692Chembridge (Drug-like library)283.321783.19813
SGTC_2871903851012.99 μM0.31254945164Chembridge (Drug-like library)283.321783.19813
SGTC_2986902244571.43 μM0.3114756459286Chembridge (Drug-like library)285.337663.30813
SGTC_2999906976571.43 μM0.3114752097066Chembridge (Drug-like library)285.337663.30813amide catabolism
SGTC_3180910928749.47 μM0.31147518891271Chembridge (Drug-like library)311.783862.97214
SGTC_3205911348349.47 μM0.31147517018033Chembridge (Drug-like library)271.311083.06813