7980721

2-[(3-methylsulfanyl-1,2,4-thiadiazol-5-yl)sulfanyl]-1-pyrrolidin-1-ylethanone

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2331
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 2981221
SMILES CSC1=NSC(=N1)SCC(=O)N2CCCC2
Standardized SMILES CSc1nsc(SCC(=O)N2CCCC2)n1
Molecular weight 275.414
ALogP 2.03
H-bond donor count 0
H-bond acceptor count 6
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 18.9
% growth inhibition (Hom. pool) 6.64


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2981221
Download HIP data (tab-delimited text)  (excel)
Gene:ACS2(YLR153C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ALG13(YGL047W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.05||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ARP2(YDL029W)|FD-Score:-3.78|P-value:7.88E-5|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:COF1(YLL050C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.07||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CWC25(YNL245C)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.03||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DIS3(YOL021C)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.08||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DUT1(YBR252W)|FD-Score:6.16|P-value:3.68E-10|Clearance:0.41||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:FAS1(YKL182W)|FD-Score:-4.53|P-value:2.94E-6|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FRS1(YLR060W)|FD-Score:4.34|P-value:7.06E-6|Clearance:0.06||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:LCB2(YDR062W)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MOB1(YIL106W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.17||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NAN1(YPL126W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.13||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NDD1(YOR372C)|FD-Score:-4.38|P-value:6.05E-6|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NIP1(YMR309C)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.18||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:ORC5(YNL261W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:OST2(YOR103C)|FD-Score:-4.46|P-value:4.11E-6|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:6.4|P-value:7.74E-11|Clearance:0.22||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE5(YMR314W)|FD-Score:3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP5(YBR237W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.12||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPA43(YOR340C)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC82(YPR190C)|FD-Score:4.28|P-value:9.42E-6|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPS13(YDR064W)|FD-Score:7.56|P-value:2.02E-14|Clearance:1.16||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS5(YJR123W)|FD-Score:5|P-value:2.88E-7|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC31(YDL195W)|FD-Score:-4.16|P-value:1.62E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC39(YLR440C)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.14||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SGV1(YPR161C)|FD-Score:-3.76|P-value:8.52E-5|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMD2(YLR275W)|FD-Score:5.75|P-value:4.42E-9|Clearance:0.48||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPC34(YKR037C)|FD-Score:-3.2|P-value:6.97E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:YKL083W(YKL083W_d)|FD-Score:6.18|P-value:3.22E-10|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR140W(YLR140W_d)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YOR146W(YOR146W_d)|FD-Score:5.28|P-value:6.63E-8|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C Gene:ACS2(YLR153C)|FD-Score:-3.18|P-value:7.32E-4|Clearance:0||SGD DESC:Acetyl-coA synthetase isoform which, along with Acs1p, is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions Gene:ALG13(YGL047W)|FD-Score:3.29|P-value:4.98E-4|Clearance:0.05||SGD DESC:Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress Gene:ARP2(YDL029W)|FD-Score:-3.78|P-value:7.88E-5|Clearance:0||SGD DESC:Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity Gene:COF1(YLL050C)|FD-Score:3.12|P-value:8.92E-4|Clearance:0.07||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:CWC25(YNL245C)|FD-Score:3.77|P-value:8.31E-5|Clearance:0.03||SGD DESC:Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p Gene:DIS3(YOL021C)|FD-Score:3.24|P-value:6.01E-4|Clearance:0.08||SGD DESC:Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3; protein abundance increases in response to DNA replication stress Gene:DUT1(YBR252W)|FD-Score:6.16|P-value:3.68E-10|Clearance:0.41||SGD DESC:deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate Gene:FAS1(YKL182W)|FD-Score:-4.53|P-value:2.94E-6|Clearance:0||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FRS1(YLR060W)|FD-Score:4.34|P-value:7.06E-6|Clearance:0.06||SGD DESC:Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control Gene:LCB2(YDR062W)|FD-Score:-3.23|P-value:6.15E-4|Clearance:0||SGD DESC:Component of serine palmitoyltransferase, responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine Gene:MOB1(YIL106W)|FD-Score:3.59|P-value:1.66E-4|Clearance:0.17||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NAN1(YPL126W)|FD-Score:3.42|P-value:3.09E-4|Clearance:0.13||SGD DESC:U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA Gene:NDD1(YOR372C)|FD-Score:-4.38|P-value:6.05E-6|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:NIP1(YMR309C)|FD-Score:4.52|P-value:3.04E-6|Clearance:0.18||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:ORC5(YNL261W)|FD-Score:4.92|P-value:4.24E-7|Clearance:0.4||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:OST2(YOR103C)|FD-Score:-4.46|P-value:4.11E-6|Clearance:0||SGD DESC:Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins Gene:PBN1(YCL052C)|FD-Score:6.4|P-value:7.74E-11|Clearance:0.22||SGD DESC:Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X Gene:PRE5(YMR314W)|FD-Score:3.73|P-value:9.47E-5|Clearance:0||SGD DESC:Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRP5(YBR237W)|FD-Score:3.89|P-value:5.11E-5|Clearance:0.12||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPA43(YOR340C)|FD-Score:3.16|P-value:7.93E-4|Clearance:0.03||SGD DESC:RNA polymerase I subunit A43 Gene:RPB5(YBR154C)|FD-Score:4.11|P-value:2.02E-5|Clearance:0.22||SGD DESC:RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation Gene:RPC82(YPR190C)|FD-Score:4.28|P-value:9.42E-6|Clearance:0.17||SGD DESC:RNA polymerase III subunit C82 Gene:RPS13(YDR064W)|FD-Score:7.56|P-value:2.02E-14|Clearance:1.16||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPS5(YJR123W)|FD-Score:5|P-value:2.88E-7|Clearance:0.08||SGD DESC:Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7 Gene:SEC31(YDL195W)|FD-Score:-4.16|P-value:1.62E-5|Clearance:0||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC39(YLR440C)|FD-Score:3.73|P-value:9.53E-5|Clearance:0.14||SGD DESC:Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope Gene:SGV1(YPR161C)|FD-Score:-3.76|P-value:8.52E-5|Clearance:0||SGD DESC:Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins Gene:SMD2(YLR275W)|FD-Score:5.75|P-value:4.42E-9|Clearance:0.48||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SPC34(YKR037C)|FD-Score:-3.2|P-value:6.97E-4|Clearance:0||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:YKL083W(YKL083W_d)|FD-Score:6.18|P-value:3.22E-10|Clearance:0.02||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR140W(YLR140W_d)|FD-Score:-4.72|P-value:1.18E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I Gene:YOR146W(YOR146W_d)|FD-Score:5.28|P-value:6.63E-8|Clearance:0.28||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2981221
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABZ2(YMR289W)|FD-Score:-4.97|P-value:3.43E-7||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACE2(YLR131C)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACK1(YDL203C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADH2(YMR303C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AFG1(YEL052W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:AIM6(YDL237W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ATE1(YGL017W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BMH1(YER177W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNI1(YNL271C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRE2(YLR015W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BSC4(YNL269W)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CDC10(YCR002C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CLN2(YPL256C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMK1(YFR014C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CST6(YIL036W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DUS4(YLR405W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ECM31(YBR176W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ECT1(YGR007W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EOS1(YNL080C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FIT1(YDR534C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMC1(YIL098C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GAS1(YMR307W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GAS3(YMR215W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GCN4(YEL009C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEA2(YEL022W)|FD-Score:4.9|P-value:4.85E-7||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAL5(YJL165C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IFM1(YOL023W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IMA1(YGR287C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IME4(YGL192W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC5(YFR038W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU1(YPL135W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KAR4(YCL055W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:LCB5(YLR260W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MBB1(YJL199C_d)|FD-Score:6.77|P-value:6.52E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MLH2(YLR035C)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNS1(YJR131W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOT3(YMR070W)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRM1(YOR201C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP2(YPR166C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT1(YIL164C)|FD-Score:-4.66|P-value:1.61E-6||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NSG1(YHR133C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OCT1(YKL134C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PAN3(YKL025C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX4(YGR133W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PGD1(YGL025C)|FD-Score:-7.87|P-value:1.71E-15||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHM6(YDR281C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PIF1(YML061C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:-4.76|P-value:9.83E-7||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMC1(YGL006W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:RHO5(YNL180C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RML2(YEL050C)|FD-Score:-8.18|P-value:1.45E-16||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM1(YGR070W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL35A(YDL191W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SAW1(YAL027W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SBH1(YER087C-B)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SER2(YGR208W)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFT2(YBL102W)|FD-Score:-5.97|P-value:1.22E-9||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SIS2(YKR072C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNX4(YJL036W)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPG3(YDR504C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRN2(YLR119W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SWI4(YER111C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYM1(YLR251W)|FD-Score:4.29|P-value:8.97E-6||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TFB5(YDR079C-A)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TRP2(YER090W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.27|P-value:6.90E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBR2(YLR024C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VAC17(YCL063W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAC8(YEL013W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPH1(YOR270C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS55(YJR044C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YDL068W(YDL068W_d)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDL186W(YDL186W_p)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER087C-A(YER087C-A_d)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER186C(YER186C_p)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL028W(YIL028W_d)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR232W(YLR232W_d)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR084W(YMR084W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOL107W(YOL107W_p)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR199W(YOR199W_d)|FD-Score:-4.32|P-value:7.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR076W(YPR076W_d)|FD-Score:5.73|P-value:5.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YRA2(YKL214C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YTA6(YPL074W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:AAD3(YCR107W)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:ABZ2(YMR289W)|FD-Score:-4.97|P-value:3.43E-7||SGD DESC:Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis Gene:ACE2(YLR131C)|FD-Score:3.75|P-value:9.01E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:ACK1(YDL203C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria Gene:ADH2(YMR303C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Glucose-repressible alcohol dehydrogenase II, catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1 Gene:AFG1(YEL052W)|FD-Score:-3.5|P-value:2.36E-4||SGD DESC:Conserved protein that may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain Gene:AIM6(YDL237W)|FD-Score:4.52|P-value:3.09E-6||SGD DESC:Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene Gene:ATE1(YGL017W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway Gene:BMH1(YER177W)|FD-Score:3.66|P-value:1.26E-4||SGD DESC:14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BNI1(YNL271C)|FD-Score:3.37|P-value:3.70E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRE2(YLR015W)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L Gene:BSC4(YNL269W)|FD-Score:4.6|P-value:2.09E-6||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CDC10(YCR002C)|FD-Score:3.16|P-value:7.97E-4||SGD DESC:Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress Gene:CLN2(YPL256C)|FD-Score:4.6|P-value:2.12E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMK1(YFR014C)|FD-Score:3.26|P-value:5.55E-4||SGD DESC:Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication Gene:COQ10(YOL008W)|FD-Score:3.17|P-value:7.73E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CST6(YIL036W)|FD-Score:3.38|P-value:3.64E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor, in ATF/CREB family; mediates transcriptional activation of NCE103 (encoding carbonic anhydrase) in response to low CO2 levels such as in the ambient air; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; CST6 has a paralog, ACA1, that arose from the whole genome duplication Gene:DGR1(YNL130C-A_p)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DUS4(YLR405W)|FD-Score:-3.44|P-value:2.90E-4||SGD DESC:Dihydrouridine synthase, member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p Gene:ECM31(YBR176W)|FD-Score:-3.1|P-value:9.58E-4||SGD DESC:Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate Gene:ECT1(YGR007W)|FD-Score:3.76|P-value:8.37E-5||SGD DESC:Ethanolamine-phosphate cytidylyltransferase, catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases Gene:EOS1(YNL080C)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene Gene:FIT1(YDR534C)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall Gene:FMC1(YIL098C)|FD-Score:-3.13|P-value:8.88E-4||SGD DESC:Mitochondrial matrix protein, required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p Gene:GAS1(YMR307W)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:GAS3(YMR215W)|FD-Score:4.61|P-value:1.98E-6||SGD DESC:Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation Gene:GCN4(YEL009C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GEA2(YEL022W)|FD-Score:4.9|P-value:4.85E-7||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:HAL5(YJL165C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication Gene:IFA38(YBR159W)|FD-Score:-3.25|P-value:5.81E-4||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IFM1(YOL023W)|FD-Score:3.99|P-value:3.35E-5||SGD DESC:Mitochondrial translation initiation factor 2 Gene:IMA1(YGR287C)|FD-Score:3.33|P-value:4.29E-4||SGD DESC:Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; specificity for isomaltose, alpha-methylglucoside, and palatinose; member of the IMA isomaltase family Gene:IME4(YGL192W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Probable mRNA N6-adenosine methyltransferase required for entry into meiosis; transcribed in diploid cells; haploids repress IME4 transcription via production of antisense IME4 transcripts; antisense transcription is repressed in diploids Gene:IRC5(YFR038W)|FD-Score:4.35|P-value:6.94E-6||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:ISU1(YPL135W)|FD-Score:3.34|P-value:4.15E-4||SGD DESC:Conserved protein of the mitochondrial matrix, performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); isu1 isu2 double mutant is inviable Gene:KAR4(YCL055W)|FD-Score:3.69|P-value:1.10E-4||SGD DESC:Transcription factor required for gene regulation in response to pheromones; also required during meiosis; exists in two forms, a slower-migrating form more abundant during vegetative growth and a faster-migrating form induced by pheromone Gene:LCB5(YLR260W)|FD-Score:-4.06|P-value:2.41E-5||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MBB1(YJL199C_d)|FD-Score:6.77|P-value:6.52E-12||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies Gene:MLH2(YLR035C)|FD-Score:3.72|P-value:9.98E-5||SGD DESC:Protein involved in the mismatch repair of certain frameshift intermediates and involved in meiotic recombination; forms a complex with Mlh1p Gene:MNS1(YJR131W)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOT3(YMR070W)|FD-Score:-4.68|P-value:1.43E-6||SGD DESC:Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion Gene:MRM1(YOR201C)|FD-Score:3.65|P-value:1.30E-4||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP2(YPR166C)|FD-Score:-3.36|P-value:3.85E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:NIT1(YIL164C)|FD-Score:-4.66|P-value:1.61E-6||SGD DESC:Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene Gene:NSG1(YHR133C)|FD-Score:3.38|P-value:3.68E-4||SGD DESC:Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress Gene:OCT1(YKL134C)|FD-Score:3.31|P-value:4.58E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:PAN3(YKL025C)|FD-Score:-3.29|P-value:4.94E-4||SGD DESC:Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes Gene:PEX4(YGR133W)|FD-Score:3.35|P-value:4.00E-4||SGD DESC:Peroxisomal ubiquitin conjugating enzyme required for peroxisomal matrix protein import and peroxisome biogenesis Gene:PGD1(YGL025C)|FD-Score:-7.87|P-value:1.71E-15||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:PHM6(YDR281C)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Protein of unknown function, expression is regulated by phosphate levels Gene:PIF1(YML061C)|FD-Score:4.85|P-value:6.17E-7||SGD DESC:DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells Gene:PIH1(YHR034C)|FD-Score:-4.76|P-value:9.83E-7||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMC1(YGL006W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in the presence of elevated concentrations of calcium; similar to mammalian PMCA1a Gene:RHO5(YNL180C)|FD-Score:-3.46|P-value:2.72E-4||SGD DESC:Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, likely involved in protein kinase C (Pkc1p)-dependent signal transduction pathway that controls cell integrity Gene:RML2(YEL050C)|FD-Score:-8.18|P-value:1.45E-16||SGD DESC:Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor Gene:ROM1(YGR070W)|FD-Score:-3.64|P-value:1.37E-4||SGD DESC:GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP Gene:RPL35A(YDL191W)|FD-Score:3.68|P-value:1.18E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPS24B(YIL069C)|FD-Score:3.22|P-value:6.43E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication Gene:RPS28B(YLR264W)|FD-Score:-3.34|P-value:4.15E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication Gene:RPS8A(YBL072C)|FD-Score:3.62|P-value:1.45E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication Gene:SAW1(YAL027W)|FD-Score:4.45|P-value:4.27E-6||SGD DESC:5'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:SBH1(YER087C-B)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Beta subunit of the Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; homologous to Sbh2p Gene:SER2(YGR208W)|FD-Score:4.45|P-value:4.39E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SFT2(YBL102W)|FD-Score:-5.97|P-value:1.22E-9||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SIS2(YKR072C)|FD-Score:3.21|P-value:6.55E-4||SGD DESC:Negative regulatory subunit of protein phosphatase 1 Ppz1p and also a subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex, which catalyzes the third step of coenzyme A biosynthesis Gene:SNX4(YJL036W)|FD-Score:4.65|P-value:1.62E-6||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPG3(YDR504C)|FD-Score:-3.54|P-value:2.00E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources Gene:SRN2(YLR119W)|FD-Score:3.1|P-value:9.60E-4||SGD DESC:Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus Gene:SWI4(YER111C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p Gene:SYM1(YLR251W)|FD-Score:4.29|P-value:8.97E-6||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TFB5(YDR079C-A)|FD-Score:4.38|P-value:5.97E-6||SGD DESC:Component of the RNA polymerase II general transcription and DNA repair factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair Gene:TRP2(YER090W)|FD-Score:4.38|P-value:5.88E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:4.61|P-value:2.01E-6||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:5.27|P-value:6.90E-8||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:UBR2(YLR024C)|FD-Score:3.95|P-value:3.98E-5||SGD DESC:Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex Gene:VAC17(YCL063W)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p Gene:VAC8(YEL013W)|FD-Score:5.89|P-value:1.90E-9||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VPH1(YOR270C)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS55(YJR044C)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YDL068W(YDL068W_d)|FD-Score:3.23|P-value:6.21E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.23|P-value:6.26E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDL186W(YDL186W_p)|FD-Score:-3.25|P-value:5.70E-4||SGD DESC:Putative protein of unknown function; YDL186W is not an essential gene Gene:YDR008C(YDR008C_d)|FD-Score:3.39|P-value:3.45E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER087C-A(YER087C-A_d)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase Gene:YER186C(YER186C_p)|FD-Score:-4.07|P-value:2.38E-5||SGD DESC:Putative protein of unknown function Gene:YGR291C(YGR291C_d)|FD-Score:4.96|P-value:3.52E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YIL028W(YIL028W_d)|FD-Score:-3.3|P-value:4.84E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR232W(YLR232W_d)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5 Gene:YMR084W(YMR084W_p)|FD-Score:-3.17|P-value:7.72E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YOL107W(YOL107W_p)|FD-Score:3.72|P-value:1.02E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR199W(YOR199W_d)|FD-Score:-4.32|P-value:7.90E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR338W(YOR338W_p)|FD-Score:3.25|P-value:5.75E-4||SGD DESC:Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR076W(YPR076W_d)|FD-Score:5.73|P-value:5.06E-9||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPR170C(YPR170C_d)|FD-Score:-3.39|P-value:3.48E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:YRA2(YKL214C)|FD-Score:4.65|P-value:1.64E-6||SGD DESC:Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus Gene:YTA6(YPL074W)|FD-Score:3.1|P-value:9.65E-4||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR064W7.562.02E-141.16RPS13Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15
YCL052C6.407.74E-110.22PBN1Essential component of glycosylphosphatidylinositol-mannosyltransferase I, required for the autocatalytic post-translational processing of the protease B precursor Prb1p, localizes to ER in lumenal orientation; homolog of mammalian PIG-X
YKL083W_d6.183.22E-100.02YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YBR252W6.163.68E-100.41DUT1deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate
YLR275W5.754.42E-90.48SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YOR146W_d5.286.63E-80.28YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YJR123W5.002.88E-70.08RPS5Protein component of the small (40S) ribosomal subunit; least basic of non-acidic ribosomal proteins; phosphorylated in vivo; essential for viability; homologous to mammalian ribosomal protein S5 and bacterial S7
YNL261W4.924.24E-70.40ORC5Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
YMR309C4.523.04E-60.18NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YLR060W4.347.06E-60.06FRS1Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
YPR190C4.289.42E-60.17RPC82RNA polymerase III subunit C82
YBR154C4.112.02E-50.22RPB5RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
YBR237W3.895.11E-50.12PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YNL245C3.778.31E-50.03CWC25Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
YMR314W3.739.47E-50.00PRE5Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJL199C_d6.776.52E-12MBB1_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; protein detected in large-scale protein-protein interaction studies
YEL013W5.891.90E-9VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YPR076W_d5.735.06E-9YPR076W_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YDR354W5.276.90E-8TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YGR291C_d4.963.52E-7YGR291C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YEL022W4.904.85E-7GEA2Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
YML061C4.856.17E-7PIF1DNA helicase; exists in a nuclear form that acts as a catalytic inhibitor of telomerase; and as a mitochondrial form involved in repair and recombination of mitochondrial DNA; mutations affect zinc and iron homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
YJL036W4.651.62E-6SNX4Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
YKL214C4.651.64E-6YRA2Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
YMR215W4.611.98E-6GAS3Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation
YKL211C4.612.01E-6TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNL269W4.602.09E-6BSC4Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p
YPL256C4.602.12E-6CLN2G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)
YDL237W4.523.09E-6AIM6Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene
YAL027W4.454.27E-6SAW15'- and 3'-flap DNA binding protein; stimulates the endonuclease activity of Rad1/Rad10p; involved in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during double-strand break repair via single-strand annealing; green fluorescent protein (GFP)-fusion protein localizes to the nucleus

GO enrichment analysis for SGTC_2331
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1286.25E-23SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)51508590.0943396
0.1286.42E-23SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0571429
0.1271.26E-22SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0810811
0.1193.18E-20SGTC_24885268135 45.7 μMMiscellaneous12703560.030303
0.1171.95E-19SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0595238
0.1164.21E-19SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.0491803
0.1165.07E-19SGTC_22246650123 117.6 μMChembridge (Fragment library)29083700.084507
0.1159.04E-19SGTC_21986589944 200.0 μMChembridge (Fragment library)52902680.0422535
0.1133.67E-18SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.084507
0.1134.31E-18SGTC_1114scopoletin 1.7 μMTimTec (Natural product library)52804600.047619
0.1119.98E-18SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.146667
0.1111.28E-17SGTC_507nimesulide 162.0 μMMiscellaneous44950.0422535
0.1101.79E-17SGTC_15465-methoxyflavanone 78.7 μMTimTec (Pure natural product library)1477950.0588235
0.1102.38E-17SGTC_24865283202 22.4 μMMiscellaneous13777510.03125
0.1102.53E-17SGTC_8440330-0132 15.3 μMChemDiv (Drug-like library)28280790.056338

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_3174910720449.47 μM0.2575763938802Chembridge (Drug-like library)337.86753.91413ERG2
SGTC_11604092-057010.5 μM0.2307694994975ChemDiv (Drug-like library)390.926823.82604plasma membrane duress
SGTC_22957971645200 μM0.2264152978226Chembridge (Fragment library)204.268242.09911
SGTC_3315913639568.55 μM0.22222216876844Chembridge (Drug-like library)423.960043.90928
SGTC_3327914064826.93 μM0.2222228029710Chembridge (Drug-like library)320.47613.9116
SGTC_3043909230349.47 μM0.22033925236084Chembridge (Drug-like library)299.430443.97302
SGTC_3096911746849.47 μM0.21666741316311Chembridge (Drug-like library)247.316121.1614
SGTC_21856113692200 μM0.214286874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_11333473-024750 μM0.212121711311ChemDiv (Drug-like library)308.422343.35625RSC complex & mRNA processing
SGTC_23849036245200 μM0.2105266462766Chembridge (Fragment library)261.362621.83713