| Deletion Strain | FD score | P-value | Clearance | Gene | Gene Description |
|---|
| YHR088W | 8.04 | 4.34E-16 | 2.00 | RPF1 | Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA |
| YML069W | 6.04 | 7.65E-10 | 0.38 | POB3 | Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress |
| YBL074C | 5.66 | 7.66E-9 | 0.67 | AAR2 | Component of the U5 snRNP, required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron |
| YLR440C | 4.98 | 3.10E-7 | 0.57 | SEC39 | Component of the Dsl1p tethering complex that interacts with ER SNAREs Sec20p and Use1p; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope |
| YDR236C | 4.42 | 5.01E-6 | 0.07 | FMN1 | Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane |
| YOR260W | 4.35 | 6.83E-6 | 0.18 | GCD1 | Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression |
| YIL083C | 4.17 | 1.52E-5 | 0.06 | CAB2 | Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity) |
| YDR454C | 4.11 | 2.01E-5 | 0.08 | GUK1 | Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins |
| YNL245C | 4.02 | 2.85E-5 | 0.03 | CWC25 | Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p |
| YLR275W | 4.00 | 3.22E-5 | 0.21 | SMD2 | Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 |
| YFL024C | 3.78 | 7.79E-5 | 4.18E-4 | EPL1 | Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb |
| YPL237W | 3.78 | 7.81E-5 | 0.10 | SUI3 | Beta subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding |
| YKL083W_d | 3.68 | 1.18E-4 | 0.01 | YKL083W_d | Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14 |
| YDR468C | 3.67 | 1.20E-4 | 0.16 | TLG1 | Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p |
| YGR098C | 3.51 | 2.21E-4 | 0.03 | ESP1 | Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress |
