9001819

5-chloro-1-ethylindole-2,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2336
Screen concentration 198.7 μM
Source Chembridge (Fragment library)
PubChem CID 2989602
SMILES CCN1C2=C(C=C(C=C2)Cl)C(=O)C1=O
Standardized SMILES CCN1C(=O)C(=O)c2cc(Cl)ccc12
Molecular weight 209.629
ALogP 1.89
H-bond donor count 0
H-bond acceptor count 2
Response signature heme biosynthesis & mitochondrial translocase

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.26
% growth inhibition (Hom. pool) 7.99


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2989602
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:7.86|P-value:1.91E-15|Clearance:0.77||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.38|P-value:3.73E-8|Clearance:0.29||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:9.55|P-value:6.33E-22|Clearance:0.25||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC27(YBL084C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.08||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC36(YDL165W)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:-4.62|P-value:1.94E-6|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:7.93|P-value:1.06E-15|Clearance:0.02||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CMD1(YBR109C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.01||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:CRM1(YGR218W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.18||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DBP2(YNL112W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.04||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG29(YMR134W_p)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FOL2(YGR267C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.01||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GCD2(YGR083C)|FD-Score:-3.27|P-value:5.38E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GDI1(YER136W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.03||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI8(YDR331W)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GUK1(YDR454C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.06||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:HEM12(YDR047W)|FD-Score:20|P-value:3.32E-89|Clearance:4.29||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM2(YGL040C)|FD-Score:5.77|P-value:3.93E-9|Clearance:0.39||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:16.4|P-value:7.14E-61|Clearance:4.29||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:-5.11|P-value:1.65E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCD4(YKL165C)|FD-Score:10.3|P-value:3.76E-25|Clearance:0.74||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MIA40(YKL195W)|FD-Score:8.45|P-value:1.48E-17|Clearance:0.51||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MOB1(YIL106W)|FD-Score:4.43|P-value:4.74E-6|Clearance:0.07||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOC2(YOR206W)|FD-Score:-4.94|P-value:3.97E-7|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP2(YNL061W)|FD-Score:-4|P-value:3.16E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP58(YOR310C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:11|P-value:1.37E-28|Clearance:0.7||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NOP9(YJL010C)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PKC1(YBL105C)|FD-Score:11.2|P-value:3.36E-29|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RER2(YBR002C)|FD-Score:10.3|P-value:2.69E-25|Clearance:0.03||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIM2(YBR192W)|FD-Score:6.6|P-value:2.02E-11|Clearance:0.12||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:5.06|P-value:2.11E-7|Clearance:0.29||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:3.78|P-value:7.98E-5|Clearance:0.01||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:-6.07|P-value:6.35E-10|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL25(YOL127W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN2(YIL075C)|FD-Score:5.08|P-value:1.84E-7|Clearance:0||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:17.8|P-value:6.09E-71|Clearance:4.29||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:4.49|P-value:3.61E-6|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-6.26|P-value:1.97E-10|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPT3(YDR394W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.02||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:9.29|P-value:7.56E-21|Clearance:0.55||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:6.48|P-value:4.53E-11|Clearance:0.6||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC3(YDR303C)|FD-Score:7.91|P-value:1.29E-15|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSC4(YKR008W)|FD-Score:4.11|P-value:1.95E-5|Clearance:0.29||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC58(YLR033W)|FD-Score:15.4|P-value:4.27E-54|Clearance:4.29||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:5.88|P-value:2.01E-9|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:7.93|P-value:1.06E-15|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.08|P-value:1.85E-7|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC7(YDR170C)|FD-Score:3.72|P-value:9.83E-5|Clearance:0.09||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SFH1(YLR321C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.12||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SQT1(YIR012W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.05||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SSC1(YJR045C)|FD-Score:-3.81|P-value:6.88E-5|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:STH1(YIL126W)|FD-Score:9.3|P-value:6.80E-21|Clearance:0.01||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF8(YML114C)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFC1(YBR123C)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.06||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF35(YDR429C)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOM22(YNL131W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:8.74|P-value:1.12E-18|Clearance:0.3||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRZ1(YKR079C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.01||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:VTI1(YMR197C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.05||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:16.1|P-value:2.74E-58|Clearance:4.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:7.09|P-value:6.86E-13|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR198C(YLR198C_d)|FD-Score:-3.11|P-value:9.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YLR317W(YLR317W_d)|FD-Score:4.76|P-value:9.45E-7|Clearance:0.24||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:ARP7(YPR034W)|FD-Score:7.86|P-value:1.91E-15|Clearance:0.77||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:ARP9(YMR033W)|FD-Score:5.38|P-value:3.73E-8|Clearance:0.29||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:BET4(YJL031C)|FD-Score:9.55|P-value:6.33E-22|Clearance:0.25||SGD DESC:Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p Gene:CDC20(YGL116W)|FD-Score:-3.86|P-value:5.59E-5|Clearance:0||SGD DESC:Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress Gene:CDC27(YBL084C)|FD-Score:3.2|P-value:6.81E-4|Clearance:0.08||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CDC36(YDL165W)|FD-Score:-3.56|P-value:1.86E-4|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC39(YCR093W)|FD-Score:-4.62|P-value:1.94E-6|Clearance:0||SGD DESC:Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor Gene:CDC48(YDL126C)|FD-Score:7.93|P-value:1.06E-15|Clearance:0.02||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:CMD1(YBR109C)|FD-Score:4.15|P-value:1.64E-5|Clearance:0.01||SGD DESC:Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin Gene:CRM1(YGR218W)|FD-Score:4.36|P-value:6.51E-6|Clearance:0.18||SGD DESC:Major karyopherin, involved in export of proteins, RNAs, and ribosomal subunits from the nucleus; exportin Gene:DBP2(YNL112W)|FD-Score:3.77|P-value:8.32E-5|Clearance:0.04||SGD DESC:ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites Gene:ERG29(YMR134W_p)|FD-Score:3.28|P-value:5.14E-4|Clearance:0.08||SGD DESC:Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes Gene:FOL2(YGR267C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.01||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GCD2(YGR083C)|FD-Score:-3.27|P-value:5.38E-4|Clearance:0||SGD DESC:Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:GDI1(YER136W)|FD-Score:3.51|P-value:2.22E-4|Clearance:0.03||SGD DESC:GDP dissociation inhibitor, regulates vesicle traffic in secretory pathways by regulating the dissociation of GDP from the Sec4/Ypt/rab family of GTP binding proteins Gene:GPI8(YDR331W)|FD-Score:-4.63|P-value:1.82E-6|Clearance:0||SGD DESC:ER membrane glycoprotein subunit of the glycosylphosphatidylinositol transamidase complex that adds glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; human PIG-K protein is a functional homolog Gene:GUK1(YDR454C)|FD-Score:3.48|P-value:2.51E-4|Clearance:0.06||SGD DESC:Guanylate kinase, converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins Gene:HEM12(YDR047W)|FD-Score:20|P-value:3.32E-89|Clearance:4.29||SGD DESC:Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda Gene:HEM2(YGL040C)|FD-Score:5.77|P-value:3.93E-9|Clearance:0.39||SGD DESC:Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus Gene:HEM3(YDL205C)|FD-Score:16.4|P-value:7.14E-61|Clearance:4.29||SGD DESC:Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme Gene:HYP2(YEL034W)|FD-Score:3.42|P-value:3.14E-4|Clearance:0.05||SGD DESC:Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication Gene:IRA1(YBR140C)|FD-Score:-5.11|P-value:1.65E-7|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:MCD4(YKL165C)|FD-Score:10.3|P-value:3.76E-25|Clearance:0.74||SGD DESC:Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes Gene:MCM2(YBL023C)|FD-Score:-4.19|P-value:1.42E-5|Clearance:0||SGD DESC:Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress Gene:MIA40(YKL195W)|FD-Score:8.45|P-value:1.48E-17|Clearance:0.51||SGD DESC:Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p Gene:MOB1(YIL106W)|FD-Score:4.43|P-value:4.74E-6|Clearance:0.07||SGD DESC:Component of the mitotic exit network; associates with and is required for the activation and Cdc15p-dependent phosphorylation of the Dbf2p kinase; required for cytokinesis and cell separation; component of the CCR4 transcriptional complex; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress Gene:NOC2(YOR206W)|FD-Score:-4.94|P-value:3.97E-7|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOG1(YPL093W)|FD-Score:-3.26|P-value:5.58E-4|Clearance:0||SGD DESC:Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins Gene:NOP2(YNL061W)|FD-Score:-4|P-value:3.16E-5|Clearance:0||SGD DESC:Probable RNA m(5)C methyltransferase, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles Gene:NOP58(YOR310C)|FD-Score:-3.7|P-value:1.06E-4|Clearance:0||SGD DESC:Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA Gene:NOP8(YOL144W)|FD-Score:11|P-value:1.37E-28|Clearance:0.7||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:NOP9(YJL010C)|FD-Score:4.52|P-value:3.07E-6|Clearance:0.01||SGD DESC:Essential subunit of U3-containing 90S preribosome involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain Gene:PKC1(YBL105C)|FD-Score:11.2|P-value:3.36E-29|Clearance:0.13||SGD DESC:Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC) Gene:RER2(YBR002C)|FD-Score:10.3|P-value:2.69E-25|Clearance:0.03||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RIM2(YBR192W)|FD-Score:6.6|P-value:2.02E-11|Clearance:0.12||SGD DESC:Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family Gene:RPB3(YIL021W)|FD-Score:5.06|P-value:2.11E-7|Clearance:0.29||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPB7(YDR404C)|FD-Score:3.78|P-value:7.98E-5|Clearance:0.01||SGD DESC:RNA polymerase II subunit B16; forms two subunit dissociable complex with Rpb4p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation Gene:RPC53(YDL150W)|FD-Score:-6.07|P-value:6.35E-10|Clearance:0||SGD DESC:RNA polymerase III subunit C53 Gene:RPL25(YOL127W)|FD-Score:-3.55|P-value:1.89E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L25; primary rRNA-binding ribosomal protein component of large ribosomal subunit; binds to 25S rRNA via a conserved C-terminal motif; homologous to mammalian ribosomal protein L23A and bacterial L23 Gene:RPN2(YIL075C)|FD-Score:5.08|P-value:1.84E-7|Clearance:0||SGD DESC:Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress Gene:RPN3(YER021W)|FD-Score:17.8|P-value:6.09E-71|Clearance:4.29||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RPN5(YDL147W)|FD-Score:4.49|P-value:3.61E-6|Clearance:0.06||SGD DESC:Subunit of the COP9 signalosome (CSN) and non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein Gene:RPN6(YDL097C)|FD-Score:4.52|P-value:3.16E-6|Clearance:0.03||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RPS13(YDR064W)|FD-Score:-6.26|P-value:1.97E-10|Clearance:0||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15 Gene:RPT3(YDR394W)|FD-Score:4.18|P-value:1.47E-5|Clearance:0.02||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:9.29|P-value:7.56E-21|Clearance:0.55||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RPT6(YGL048C)|FD-Score:6.48|P-value:4.53E-11|Clearance:0.6||SGD DESC:ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress Gene:RSC3(YDR303C)|FD-Score:7.91|P-value:1.29E-15|Clearance:0.05||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; highly similar to Rsc30p Gene:RSC4(YKR008W)|FD-Score:4.11|P-value:1.95E-5|Clearance:0.29||SGD DESC:Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p Gene:RSC58(YLR033W)|FD-Score:15.4|P-value:4.27E-54|Clearance:4.29||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC6(YCR052W)|FD-Score:5.88|P-value:2.01E-9|Clearance:0.11||SGD DESC:Component of the RSC chromatin remodeling complex; essential for mitotic growth; homolog of SWI/SNF subunit Swp73p Gene:RSC8(YFR037C)|FD-Score:7.93|P-value:1.06E-15|Clearance:0||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:5.08|P-value:1.85E-7|Clearance:0.02||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:SEC7(YDR170C)|FD-Score:3.72|P-value:9.83E-5|Clearance:0.09||SGD DESC:Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles Gene:SFH1(YLR321C)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.12||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:SQT1(YIR012W)|FD-Score:3.36|P-value:3.93E-4|Clearance:0.05||SGD DESC:Protein involved in 60S ribosomal subunit assembly or modification; contains multiple WD repeats; interacts with Qsr1p in a two-hybrid assay; protein abundance increases in response to DNA replication stress Gene:SSC1(YJR045C)|FD-Score:-3.81|P-value:6.88E-5|Clearance:0||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:STH1(YIL126W)|FD-Score:9.3|P-value:6.80E-21|Clearance:0.01||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TAF8(YML114C)|FD-Score:-3.24|P-value:5.97E-4|Clearance:0||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:TFC1(YBR123C)|FD-Score:3.12|P-value:8.91E-4|Clearance:0.06||SGD DESC:One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; human homolog is TFIIIC-63 Gene:TIF35(YDR429C)|FD-Score:-3.92|P-value:4.45E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:TIM10(YHR005C-A)|FD-Score:-3.13|P-value:8.63E-4|Clearance:0||SGD DESC:Essential protein of the mitochondrial intermembrane space, forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane Gene:TOM22(YNL131W)|FD-Score:3.31|P-value:4.72E-4|Clearance:0.02||SGD DESC:Component of the TOM (translocase of outer mitochondrial membrane) complex responsible for initial import of mitochondrially directed proteins; acts as a receptor for precursor proteins and mediates interaction between TOM and TIM complexes Gene:TOM40(YMR203W)|FD-Score:8.74|P-value:1.12E-18|Clearance:0.3||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore Gene:TRZ1(YKR079C)|FD-Score:4.12|P-value:1.88E-5|Clearance:0.01||SGD DESC:tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2 Gene:VTI1(YMR197C)|FD-Score:3.83|P-value:6.46E-5|Clearance:0.05||SGD DESC:Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways Gene:YDL152W(YDL152W_d)|FD-Score:-4.16|P-value:1.57E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome Gene:YDL196W(YDL196W_d)|FD-Score:16.1|P-value:2.74E-58|Clearance:4.29||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YJL086C(YJL086C_d)|FD-Score:7.09|P-value:6.86E-13|Clearance:0.48||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL085W/EXO70 and YJL087C/TRL1 Gene:YLR198C(YLR198C_d)|FD-Score:-3.11|P-value:9.20E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:YLR317W(YLR317W_d)|FD-Score:4.76|P-value:9.45E-7|Clearance:0.24||SGD DESC:Dubious open reading frame; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:4.14|P-value:1.72E-5|Clearance:0.02||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2989602
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AFT1(YGL071W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM11(YER093C-A)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:AIM22(YJL046W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALY1(YKR021W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ANB1(YJR047C)|FD-Score:-5.31|P-value:5.34E-8||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ATG1(YGL180W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATG16(YMR159C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG29(YPL166W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG34(YOL083W)|FD-Score:6.42|P-value:7.02E-11||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:ATG36(YJL185C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP12(YJL180C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP3(YBR039W)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AYR1(YIL124W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BDH2(YAL061W_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BEM4(YPL161C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA3(YJL060W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC5(YNR069C)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD19(YJL188C_d)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCS1(YMR038C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:6.26|P-value:1.94E-10||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLN3(YAL040C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYC7(YEL039C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DID2(YKR035W-A)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DIT1(YDR403W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DSE1(YER124C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSF2(YBR007C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ESL2(YKR096W)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FAU1(YER183C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FET4(YMR319C)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE3(YOR381W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAC1(YOR178C)|FD-Score:6.72|P-value:9.38E-12||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GEA2(YEL022W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:5.74|P-value:4.70E-9||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GTR2(YGR163W)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAP4(YKL109W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HIT1(YJR055W)|FD-Score:-3.73|P-value:9.49E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HTA1(YDR225W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ILM1(YJR118C)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC3(YFR013W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JID1(YPR061C)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LRE1(YCL051W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM7(YNL147W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:5.93|P-value:1.47E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRPL6(YHR147C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST27(YGL051W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:MTD1(YKR080W)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NOP13(YNL175C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:OCT1(YKL134C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OSH3(YHR073W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OSH6(YKR003W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:OSM1(YJR051W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:-4.91|P-value:4.46E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET191(YJR034W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PEX13(YLR191W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGM3(YMR278W)|FD-Score:5.76|P-value:4.11E-9||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIH1(YHR034C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT3(YOR321W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PUS1(YPL212C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PUS6(YGR169C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:RAD18(YCR066W)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD4(YER162C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD52(YML032C)|FD-Score:4.31|P-value:8.14E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RCK2(YLR248W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFX1(YLR176C)|FD-Score:4.32|P-value:7.72E-6||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:ROX3(YBL093C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL13A(YDL082W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-6.25|P-value:2.10E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS23A(YGR118W)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS29B(YDL061C)|FD-Score:4.97|P-value:3.26E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:6.04|P-value:7.51E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRG7(YOR305W)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RRT2(YBR246W)|FD-Score:4.79|P-value:8.34E-7||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAP185(YJL098W)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SER2(YGR208W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SIL1(YOL031C)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIR1(YKR101W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SOD1(YJR104C)|FD-Score:5.78|P-value:3.81E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO21(YOL091W)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPO7(YAL009W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPR6(YER115C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:STE50(YCL032W)|FD-Score:5.42|P-value:2.95E-8||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STP1(YDR463W)|FD-Score:-4.86|P-value:5.76E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA46(YOR091W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRF5(YNL299W)|FD-Score:9.79|P-value:6.29E-23||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TUL1(YKL034W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TYW3(YGL050W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBC4(YBR082C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBI4(YLL039C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:USA1(YML029W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VHR1(YIL056W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VMA11(YPL234C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPH2(YKL119C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS20(YMR077C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAL065C(YAL065C_p)|FD-Score:5.91|P-value:1.75E-9||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YCR016W(YCR016W_p)|FD-Score:6.56|P-value:2.64E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR043C(YCR043C_p)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL120W(YJL120W_d)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR011C(YJR011C_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YMR119W-A(YMR119W-A_d)|FD-Score:4.84|P-value:6.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL296W(YNL296W_d)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL098C(YOL098C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative metalloprotease Gene:YOL107W(YOL107W_p)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR331C(YOR331C_d)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL039W(YPL039W_p)|FD-Score:-4.38|P-value:5.87E-6||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR053C(YPR053C_d)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR123C(YPR123C_d)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR170C(YPR170C_d)|FD-Score:4.34|P-value:7.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B Gene:ADE3(YGR204W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AFT1(YGL071W)|FD-Score:-4.18|P-value:1.47E-5||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM11(YER093C-A)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:AIM22(YJL046W)|FD-Score:3.11|P-value:9.30E-4||SGD DESC:Putative lipoate-protein ligase, required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss Gene:ALY1(YKR021W)|FD-Score:3.31|P-value:4.62E-4||SGD DESC:Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication Gene:ANB1(YJR047C)|FD-Score:-5.31|P-value:5.34E-8||SGD DESC:Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication Gene:ATG1(YGL180W)|FD-Score:-3.23|P-value:6.24E-4||SGD DESC:Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p Gene:ATG16(YMR159C)|FD-Score:6.28|P-value:1.72E-10||SGD DESC:Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG29(YPL166W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress Gene:ATG34(YOL083W)|FD-Score:6.42|P-value:7.02E-11||SGD DESC:Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog Gene:ATG36(YJL185C)|FD-Score:3.29|P-value:5.00E-4||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:ATP12(YJL180C)|FD-Score:3.11|P-value:9.24E-4||SGD DESC:Conserved protein required for assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; mutation of human ATP12 reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency Gene:ATP3(YBR039W)|FD-Score:4.99|P-value:3.09E-7||SGD DESC:Gamma subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis Gene:AYR1(YIL124W)|FD-Score:3.89|P-value:5.00E-5||SGD DESC:NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase found in lipid particles, ER, and mitochondrial outer membrane; involved in phosphatidic acid biosynthesis; required for spore germination; capable of metabolizing steroid hormones Gene:BDH2(YAL061W_p)|FD-Score:3.13|P-value:8.74E-4||SGD DESC:Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3 Gene:BEM4(YPL161C)|FD-Score:4.04|P-value:2.66E-5||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BNA3(YJL060W)|FD-Score:3.83|P-value:6.42E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BSC5(YNR069C)|FD-Score:4.27|P-value:9.74E-6||SGD DESC:Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro Gene:BUD19(YJL188C_d)|FD-Score:4.46|P-value:4.05E-6||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CCS1(YMR038C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:CDC50(YCR094W)|FD-Score:6.26|P-value:1.94E-10||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:CDC73(YLR418C)|FD-Score:3.97|P-value:3.53E-5||SGD DESC:Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress Gene:CLN3(YAL040C)|FD-Score:4.95|P-value:3.75E-7||SGD DESC:G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote the G1 to S phase transition; plays a role in regulating transcription of the other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis Gene:COG6(YNL041C)|FD-Score:-3.29|P-value:5.00E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CTR1(YPR124W)|FD-Score:3.67|P-value:1.23E-4||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CYC7(YEL039C)|FD-Score:-3.46|P-value:2.68E-4||SGD DESC:Cytochrome c isoform 2, expressed under hypoxic conditions; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication Gene:CYT2(YKL087C)|FD-Score:4.14|P-value:1.71E-5||SGD DESC:Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1 Gene:DID2(YKR035W-A)|FD-Score:4.17|P-value:1.53E-5||SGD DESC:Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors Gene:DIT1(YDR403W)|FD-Score:3.1|P-value:9.64E-4||SGD DESC:Sporulation-specific enzyme required for spore wall maturation, involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure Gene:DSE1(YER124C)|FD-Score:4|P-value:3.14E-5||SGD DESC:Daughter cell-specific protein, may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:DSF2(YBR007C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:4.1|P-value:2.03E-5||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:ENO1(YGR254W)|FD-Score:3.32|P-value:4.49E-4||SGD DESC:Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress Gene:ESL2(YKR096W)|FD-Score:-4.6|P-value:2.12E-6||SGD DESC:Protein of unknown function that interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; predicted to contain a PINc domain Gene:FAU1(YER183C)|FD-Score:-4.06|P-value:2.43E-5||SGD DESC:5,10-methenyltetrahydrofolate synthetase, involved in folic acid biosynthesis Gene:FET4(YMR319C)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FRE3(YOR381W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:GAC1(YOR178C)|FD-Score:6.72|P-value:9.38E-12||SGD DESC:Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock Gene:GEA2(YEL022W)|FD-Score:4.74|P-value:1.06E-6||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GET2(YER083C)|FD-Score:5.74|P-value:4.70E-9||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GTR2(YGR163W)|FD-Score:-3.99|P-value:3.36E-5||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:HAP4(YKL109W)|FD-Score:4.44|P-value:4.45E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex Gene:HIT1(YJR055W)|FD-Score:-3.73|P-value:9.49E-5||SGD DESC:Protein of unknown function, required for growth at high temperature Gene:HTA1(YDR225W)|FD-Score:3.4|P-value:3.35E-4||SGD DESC:Histone H2A, core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p Gene:ILM1(YJR118C)|FD-Score:4.74|P-value:1.04E-6||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC3(YFR013W)|FD-Score:-3.24|P-value:5.99E-4||SGD DESC:Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; IOC3 has a paralog, ESC8, that arose from the whole genome duplication Gene:IRC21(YMR073C)|FD-Score:4.49|P-value:3.48E-6||SGD DESC:Putative protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study Gene:JID1(YPR061C)|FD-Score:4.66|P-value:1.55E-6||SGD DESC:Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae Gene:LRE1(YCL051W)|FD-Score:-3.53|P-value:2.09E-4||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:LSM7(YNL147W)|FD-Score:3.09|P-value:9.93E-4||SGD DESC:Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress Gene:MAC1(YMR021C)|FD-Score:5.93|P-value:1.47E-9||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MRPL6(YHR147C)|FD-Score:3.25|P-value:5.79E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MST27(YGL051W)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:MTD1(YKR080W)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NOP13(YNL175C)|FD-Score:3.36|P-value:3.84E-4||SGD DESC:Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress Gene:OCT1(YKL134C)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Mitochondrial intermediate peptidase, cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis Gene:OSH3(YHR073W)|FD-Score:-4.2|P-value:1.34E-5||SGD DESC:Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability Gene:OSH6(YKR003W)|FD-Score:-4.01|P-value:3.04E-5||SGD DESC:Member of an oxysterol-binding protein family; overlapping, redundant functions in sterol metabolism and which collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion Gene:OSM1(YJR051W)|FD-Score:-3.92|P-value:4.38E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PDR1(YGL013C)|FD-Score:-4.91|P-value:4.46E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PET191(YJR034W)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PEX13(YLR191W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p Gene:PGM3(YMR278W)|FD-Score:5.76|P-value:4.11E-9||SGD DESC:Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential Gene:PIH1(YHR034C)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II Gene:PMT3(YOR321W)|FD-Score:4.75|P-value:1.03E-6||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:PRS2(YER099C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication Gene:PSP2(YML017W)|FD-Score:4.93|P-value:4.14E-7||SGD DESC:Asn rich cytoplasmic protein that contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing Gene:PUS1(YPL212C)|FD-Score:3.17|P-value:7.70E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:PUS6(YGR169C)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:RAD18(YCR066W)|FD-Score:3.91|P-value:4.71E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD4(YER162C)|FD-Score:3.86|P-value:5.74E-5||SGD DESC:Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins Gene:RAD52(YML032C)|FD-Score:4.31|P-value:8.14E-6||SGD DESC:Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis Gene:RCK2(YLR248W)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication Gene:RCY1(YJL204C)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RFX1(YLR176C)|FD-Score:4.32|P-value:7.72E-6||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:ROX3(YBL093C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPL13A(YDL082W)|FD-Score:-3.22|P-value:6.48E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL2A(YFR031C-A)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication Gene:RPS18A(YDR450W)|FD-Score:-6.25|P-value:2.10E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress Gene:RPS23A(YGR118W)|FD-Score:-3.3|P-value:4.81E-4||SGD DESC:Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal Gene:RPS29B(YDL061C)|FD-Score:4.97|P-value:3.26E-7||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication Gene:RPS4B(YHR203C)|FD-Score:6.04|P-value:7.51E-10||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication Gene:RRG1(YDR065W)|FD-Score:-3.14|P-value:8.37E-4||SGD DESC:Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RRG7(YOR305W)|FD-Score:4.42|P-value:4.96E-6||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene Gene:RRT2(YBR246W)|FD-Score:4.79|P-value:8.34E-7||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RSM28(YDR494W)|FD-Score:-3.45|P-value:2.80E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation Gene:SAP185(YJL098W)|FD-Score:4.38|P-value:6.03E-6||SGD DESC:Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication Gene:SBE22(YHR103W)|FD-Score:3.25|P-value:5.68E-4||SGD DESC:Protein involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; involved in bud growth Gene:SER2(YGR208W)|FD-Score:5.14|P-value:1.39E-7||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SIL1(YOL031C)|FD-Score:4.5|P-value:3.44E-6||SGD DESC:Nucleotide exchange factor for the endoplasmic reticulum (ER) lumenal Hsp70 chaperone Kar2p, required for protein translocation into the ER; homolog of Yarrowia lipolytica SLS1; GrpE-like protein Gene:SIR1(YKR101W)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Protein involved in repression of transcription at the silent mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin Gene:SOD1(YJR104C)|FD-Score:5.78|P-value:3.81E-9||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SOD2(YHR008C)|FD-Score:3.22|P-value:6.49E-4||SGD DESC:Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated Gene:SPO21(YOL091W)|FD-Score:-4.45|P-value:4.21E-6||SGD DESC:Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation Gene:SPO7(YAL009W)|FD-Score:4.03|P-value:2.82E-5||SGD DESC:Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation Gene:SPR6(YER115C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Protein of unknown function, expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation Gene:STE50(YCL032W)|FD-Score:5.42|P-value:2.95E-8||SGD DESC:Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction Gene:STP1(YDR463W)|FD-Score:-4.86|P-value:5.76E-7||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:TKL1(YPR074C)|FD-Score:3.16|P-value:7.85E-4||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:TMA46(YOR091W)|FD-Score:3.37|P-value:3.69E-4||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TRF5(YNL299W)|FD-Score:9.79|P-value:6.29E-23||SGD DESC:Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p Gene:TUL1(YKL034W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:TYW3(YGL050W)|FD-Score:-3.14|P-value:8.57E-4||SGD DESC:tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions Gene:UBC4(YBR082C)|FD-Score:3.51|P-value:2.24E-4||SGD DESC:Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication Gene:UBI4(YLL039C)|FD-Score:-3.4|P-value:3.36E-4||SGD DESC:Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress Gene:USA1(YML029W)|FD-Score:3.12|P-value:9.01E-4||SGD DESC:Scaffold subunit of the Hrd1p ubiquitin ligase that also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p Gene:VHR1(YIL056W)|FD-Score:3.23|P-value:6.30E-4||SGD DESC:Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication Gene:VMA11(YPL234C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Vacuolar ATPase V0 domain subunit c', involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen Gene:VPH2(YKL119C)|FD-Score:4.35|P-value:6.75E-6||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS20(YMR077C)|FD-Score:3.11|P-value:9.28E-4||SGD DESC:Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes Gene:YAL042C-A(YAL042C-A_d)|FD-Score:3.11|P-value:9.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene Gene:YAL065C(YAL065C_p)|FD-Score:5.91|P-value:1.75E-9||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YCR016W(YCR016W_p)|FD-Score:6.56|P-value:2.64E-11||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR043C(YCR043C_p)|FD-Score:-3.83|P-value:6.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene Gene:YDR509W(YDR509W_d)|FD-Score:4.06|P-value:2.42E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YEL020C(YEL020C_p)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data Gene:YFL041W-A(YFL041W-A_p)|FD-Score:-4.39|P-value:5.74E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YIL134C-A(YIL134C-A_p)|FD-Score:-3.18|P-value:7.48E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YJL120W(YJL120W_d)|FD-Score:4.2|P-value:1.31E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO Gene:YJR011C(YJR011C_p)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YMR119W-A(YMR119W-A_d)|FD-Score:4.84|P-value:6.41E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR196W(YMR196W_p)|FD-Score:-3.46|P-value:2.73E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR221C(YMR221C_p)|FD-Score:5.51|P-value:1.83E-8||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy Gene:YNL296W(YNL296W_d)|FD-Score:4.96|P-value:3.60E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog Gene:YOL097W-A(YOL097W-A_p)|FD-Score:-3.43|P-value:3.01E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YOL098C(YOL098C_p)|FD-Score:-3.16|P-value:7.87E-4||SGD DESC:Putative metalloprotease Gene:YOL107W(YOL107W_p)|FD-Score:4.05|P-value:2.55E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR331C(YOR331C_d)|FD-Score:-3.19|P-value:7.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W Gene:YPL039W(YPL039W_p)|FD-Score:-4.38|P-value:5.87E-6||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:4.97|P-value:3.34E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR053C(YPR053C_d)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene NHP6A/YPR052C Gene:YPR123C(YPR123C_d)|FD-Score:3.89|P-value:4.97E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YPR170C(YPR170C_d)|FD-Score:4.34|P-value:7.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORFs YPR169W-A and YPR170W-B

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR047W20.003.32E-894.29HEM12Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
YER021W17.806.09E-714.29RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YDL205C16.407.14E-614.29HEM3Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
YDL196W_d16.102.74E-584.29YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YLR033W15.404.27E-544.29RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YBL105C11.203.36E-290.13PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
YOL144W11.001.37E-280.70NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YBR002C10.302.69E-250.03RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL165C10.303.76E-250.74MCD4Protein involved in glycosylphosphatidylinositol (GPI) anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes
YJL031C9.556.33E-220.25BET4Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
YIL126W9.306.80E-210.01STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YOR117W9.297.56E-210.55RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YMR203W8.741.12E-180.30TOM40Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
YKL195W8.451.48E-170.51MIA40Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
YFR037C7.931.06E-155.76E-4RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNL299W9.796.29E-23TRF5Non-canonical poly(A) polymerase, involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
YOR178C6.729.38E-12GAC1Regulatory subunit for Glc7p type-1 protein phosphatase (PP1), tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock
YCR016W_p6.562.64E-11YCR016W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis
YOL083W6.427.02E-11ATG34Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
YMR159C6.281.72E-10ATG16Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which localize to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
YCR094W6.261.94E-10CDC50Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
YHR203C6.047.51E-10RPS4BProtein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
YMR021C5.931.47E-9MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YAL065C_p5.911.75E-9YAL065C_pPutative protein of unknown function; has homology to FLO1; possible pseudogene
YJR104C5.783.81E-9SOD1Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
YMR278W5.764.11E-9PGM3Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
YER083C5.744.70E-9GET2Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
YMR221C_p5.511.83E-8YMR221C_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy
YCL032W5.422.95E-8STE50Protein involved in mating response, invasive/filamentous growth, and osmotolerance, acts as an adaptor that links G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction
YGR208W5.141.39E-7SER2Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source

GO enrichment analysis for SGTC_2336
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3968.29E-221SGTC_1113artemisinin 1.2 μMTimTec (Natural product library)22400.0634921heme biosynthesis & mitochondrial translocase
0.3763.27E-197SGTC_2519cryptotanshinone 88.0 μMMicrosource (Natural product library)1602540.125heme biosynthesis & mitochondrial translocase
0.3679.36E-188SGTC_1098clofazimine 15.1 μMNIH Clinical Collection27940.144928heme biosynthesis & mitochondrial translocase
0.3552.88E-175SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.166667superoxide
0.3512.13E-170SGTC_20665222451 176.0 μMChembridge (Fragment library)22811470.306122heme biosynthesis & mitochondrial translocase
0.3171.30E-137SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.150943superoxide
0.3124.32E-133SGTC_553k007-0256 45.7 μMChemDiv (Drug-like library)62870000.0727273heme biosynthesis & mitochondrial translocase
0.3111.11E-132SGTC_2575plumbagin 510.0 nMTimTec (Pure natural product library)102050.166667superoxide
0.3111.18E-132SGTC_7450469-0705 296.0 μMChemDiv (Drug-like library)34867360.175439mitochondrial processes
0.3094.69E-131SGTC_21245246320 200.0 μMChembridge (Fragment library)5793420.0740741heme biosynthesis & mitochondrial translocase
0.3076.47E-129SGTC_2617dihydrotanshinone i 20.8 μMMicrosource (Natural product library)53167430.133333copper-dependent oxidative stress
0.3021.53E-124SGTC_22256654152 15.4 μMChembridge (Fragment library)975820.108696
0.2991.15E-122SGTC_29289011484 9.8 μMChembridge (Drug-like library)29947510.129032heme biosynthesis & mitochondrial translocase
0.2978.82E-121SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.116667heme biosynthesis & mitochondrial translocase
0.2822.83E-108SGTC_14534237-0541 195.0 μMChemDiv (Drug-like library)7398920.0793651heme biosynthesis & mitochondrial translocase

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20024023503131 μM0.6571434736863Chembridge (Fragment library)193.1744231.4303mitochondrial processes
SGTC_2001402350257.66 μM0.617647108941Chembridge (Fragment library)195.602441.5402mitochondrial processes
SGTC_349k015-001424 μM0.4347832196908ChemDiv (Drug-like library)350.594463.87202
SGTC_10483161-030024.5 μM0.4186052165101ChemDiv (Drug-like library)266.090722.24102
SGTC_1028k015-00294.8 μM0.44588309ChemDiv (Drug-like library)351.141023.68304
SGTC_606k015-000838.6 μM0.3953491970475ChemDiv (Drug-like library)306.143463.78802
SGTC_169k015-001716.03 μM0.3913041840002ChemDiv (Drug-like library)334.1398633.41303
SGTC_1458k015-00346.33 μM0.3913043598957ChemDiv (Drug-like library)352.1303263.61904
SGTC_20225136654200 μM0.3902441713771Chembridge (Fragment library)204.225180.99703
SGTC_295k015-002731 μM0.3877553887424ChemDiv (Drug-like library)296.277482.8404PDR1