9009472

(2Z)-2-[(3-methylphenyl)methylidene]-1-azabicyclo[2.2.2]octan-3-ol

[PubChem] [DrugBank] [Wikipedia] [WikiGenes] [ChEMBL] [ChemSpider] 



Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2337
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 5742383
SMILES CC1=CC=CC(=C1)C=C2C(C3CCN2CC3)O
Standardized SMILES Cc1cccc(C=C2C(O)C3CCN2CC3)c1
Molecular weight 229.3175
ALogP 2.35
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.67
% growth inhibition (Hom. pool) 4.53


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5742383
Download HIP data (tab-delimited text)  (excel)
Gene:ARP7(YPR034W)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC12(YHR107C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.19||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CUS1(YMR240C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.01||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:CWC22(YGR278W)|FD-Score:-8.28|P-value:6.08E-17|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:EPL1(YFL024C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.05||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERG12(YMR208W)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.06||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:HAS1(YMR290C)|FD-Score:-3.19|P-value:6.99E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KAP95(YLR347C)|FD-Score:-3.38|P-value:3.66E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MES1(YGR264C)|FD-Score:3.25|P-value:5.80E-4|Clearance:0.04||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MYO1(YHR023W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.01||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NDD1(YOR372C)|FD-Score:-8.08|P-value:3.35E-16|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:PDI1(YCL043C)|FD-Score:4.27|P-value:9.79E-6|Clearance:0.07||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP22(YER013W)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.02||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RAP1(YNL216W)|FD-Score:3.85|P-value:5.90E-5|Clearance:0.31||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RER2(YBR002C)|FD-Score:5.71|P-value:5.63E-9|Clearance:0.12||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC11(YDR045C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.3||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RTP1(YMR185W_p)|FD-Score:5.06|P-value:2.11E-7|Clearance:0.79||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SPN1(YPR133C)|FD-Score:8.39|P-value:2.35E-17|Clearance:2.68||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP14(YDL092W)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TFB2(YPL122C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.09||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YDL196W(YDL196W_d)|FD-Score:-4.71|P-value:1.21E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YKL083W(YKL083W_d)|FD-Score:5.6|P-value:1.10E-8|Clearance:0.54||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:ARP7(YPR034W)|FD-Score:3.47|P-value:2.63E-4|Clearance:0.05||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:CDC12(YHR107C)|FD-Score:3.21|P-value:6.71E-4|Clearance:0.19||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CUS1(YMR240C)|FD-Score:3.54|P-value:2.02E-4|Clearance:0.01||SGD DESC:Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p Gene:CWC22(YGR278W)|FD-Score:-8.28|P-value:6.08E-17|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:EPL1(YFL024C)|FD-Score:3.3|P-value:4.80E-4|Clearance:0.05||SGD DESC:Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb Gene:ERG12(YMR208W)|FD-Score:-3.24|P-value:5.89E-4|Clearance:0||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:-3.22|P-value:6.37E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GCR1(YPL075W)|FD-Score:3.52|P-value:2.14E-4|Clearance:0.06||SGD DESC:Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p Gene:HAS1(YMR290C)|FD-Score:-3.19|P-value:6.99E-4|Clearance:0||SGD DESC:ATP-dependent RNA helicase; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles Gene:KAP95(YLR347C)|FD-Score:-3.38|P-value:3.66E-4|Clearance:0||SGD DESC:Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p Gene:MES1(YGR264C)|FD-Score:3.25|P-value:5.80E-4|Clearance:0.04||SGD DESC:Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs Gene:MYO1(YHR023W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.01||SGD DESC:Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively Gene:NDD1(YOR372C)|FD-Score:-8.08|P-value:3.35E-16|Clearance:0||SGD DESC:Transcriptional activator essential for nuclear division; localized to the nucleus; essential component of the mechanism that activates the expression of a set of late-S-phase-specific genes Gene:PDI1(YCL043C)|FD-Score:4.27|P-value:9.79E-6|Clearance:0.07||SGD DESC:Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication Gene:PRP22(YER013W)|FD-Score:3.41|P-value:3.20E-4|Clearance:0.02||SGD DESC:DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes Gene:RAP1(YNL216W)|FD-Score:3.85|P-value:5.90E-5|Clearance:0.31||SGD DESC:Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain) Gene:RER2(YBR002C)|FD-Score:5.71|P-value:5.63E-9|Clearance:0.12||SGD DESC:Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting Gene:RPC11(YDR045C)|FD-Score:4.15|P-value:1.68E-5|Clearance:0.3||SGD DESC:RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS Gene:RTP1(YMR185W_p)|FD-Score:5.06|P-value:2.11E-7|Clearance:0.79||SGD DESC:Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene Gene:SPN1(YPR133C)|FD-Score:8.39|P-value:2.35E-17|Clearance:2.68||SGD DESC:Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype Gene:SRP14(YDL092W)|FD-Score:-3.41|P-value:3.27E-4|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TFB2(YPL122C)|FD-Score:3.39|P-value:3.48E-4|Clearance:0.09||SGD DESC:Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH Gene:YDL196W(YDL196W_d)|FD-Score:-4.71|P-value:1.21E-6|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YKL083W(YKL083W_d)|FD-Score:5.6|P-value:1.10E-8|Clearance:0.54||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified essential gene RRP14 Gene:YLR076C(YLR076C_d)|FD-Score:4.2|P-value:1.34E-5|Clearance:0.05||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5742383
Download HOP data (tab-delimited text)  (excel)
Gene:ACO1(YLR304C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADY4(YLR227C)|FD-Score:4.78|P-value:8.96E-7||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AIM33(YML087C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:APS1(YLR170C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARA1(YBR149W)|FD-Score:6.75|P-value:7.50E-12||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARG8(YOL140W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ATG22(YCL038C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:ATP25(YMR098C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRR1(YPR057W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BUD8(YLR353W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole Gene:CAP2(YIL034C)|FD-Score:-5.63|P-value:8.81E-9||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP1(YJL209W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CLN2(YPL256C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMC1(YKL137W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:CTF8(YHR191C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL5(YJR152W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DEG1(YFL001W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DIC1(YLR348C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EFG1(YGR271C-A)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERS1(YCR075C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:ERV46(YAL042W)|FD-Score:-3.92|P-value:4.46E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FLC2(YAL053W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FPR1(YNL135C)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRE2(YKL220C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GEA2(YEL022W)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIT1(YCR098C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM3(YOL056W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTR2(YGR163W)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUD1(YDL238C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HHF1(YBR009C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HOP1(YIL072W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPM1(YIL110W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HUA1(YGR268C)|FD-Score:6.24|P-value:2.12E-10||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ICS2(YBR157C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFA38(YBR159W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMG2(YCR071C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IML3(YBR107C)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC11(YOR013W_d)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAT1(YNL071W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LEM3(YNL323W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LRE1(YCL051W)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MCX1(YBR227C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDJ2(YNL328C)|FD-Score:-4.8|P-value:7.94E-7||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MNL2(YLR057W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MNS1(YJR131W)|FD-Score:6.53|P-value:3.20E-11||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOH1(YBL049W)|FD-Score:5.73|P-value:4.95E-9||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRM1(YOR201C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP13(YGR084C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL16(YBL038W)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL39(YML009C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.84|P-value:6.48E-7||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSB3(YNL293W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN2(YMR037C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MST27(YGL051W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:PDR1(YGL013C)|FD-Score:-4.91|P-value:4.49E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-4.88|P-value:5.34E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POR2(YIL114C)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PUS6(YGR169C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:PXL1(YKR090W)|FD-Score:6.06|P-value:6.92E-10||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD23(YEL037C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RFX1(YLR176C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RMD8(YFR048W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RMD9(YGL107C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPL13A(YDL082W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL1B(YGL135W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL35A(YDL191W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SER2(YGR208W)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SER33(YIL074C)|FD-Score:6.42|P-value:6.79E-11||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHS1(YDL225W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIT4(YDL047W)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLG1(YOR008C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPA2(YLL021W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPH1(YLR313C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSK1(YLR006C)|FD-Score:-4.86|P-value:5.87E-7||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SWF1(YDR126W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWR1(YDR334W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYN8(YAL014C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TED1(YIL039W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:THI72(YOR192C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TMA16(YOR252W)|FD-Score:4.86|P-value:5.98E-7||SGD DESC:Protein of unknown function that associates with ribosomes Gene:UGA2(YBR006W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UPS3(YDR185C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:URE2(YNL229C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VID28(YIL017C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPH1(YOR270C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS55(YJR044C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL039W-B(YBL039W-B_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function Gene:YCL012C(YCL012C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:-4.37|P-value:6.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:-3.72|P-value:9.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR186C(YDR186C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR433W(YDR433W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:-5.8|P-value:3.30E-9||SGD DESC:Putative protein of unknown function Gene:YGR050C(YGR050C_p)|FD-Score:8.76|P-value:9.85E-19||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL067C(YIL067C_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YKR018C(YKR018C_p)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL059C(YLL059C_d)|FD-Score:-6.88|P-value:2.92E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YNL319W(YNL319W_d)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOL107W(YOL107W_p)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR342C(YOR342C_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:-4.39|P-value:5.66E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR146C(YPR146C_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ACO1(YLR304C)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy Gene:ADY4(YLR227C)|FD-Score:4.78|P-value:8.96E-7||SGD DESC:Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane Gene:AIM33(YML087C)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Putative protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication Gene:APS1(YLR170C)|FD-Score:3.69|P-value:1.11E-4||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex Gene:ARA1(YBR149W)|FD-Score:6.75|P-value:7.50E-12||SGD DESC:NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product Gene:ARG8(YOL140W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine Gene:ATG22(YCL038C)|FD-Score:-3.58|P-value:1.70E-4||SGD DESC:Vacuolar integral membrane protein required for efflux of amino acids during autophagic body breakdown in the vacuole; null mutation causes a gradual loss of viability during starvation Gene:ATP25(YMR098C)|FD-Score:4.19|P-value:1.39E-5||SGD DESC:Mitochondrial protein required for the stability of Oli1p (Atp9p) mRNA and for the Oli1p ring formation; YMR098C is not an essential gene Gene:BNI1(YNL271C)|FD-Score:3.36|P-value:3.89E-4||SGD DESC:Formin, nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables, functionally redundant with BNR1 Gene:BRR1(YPR057W)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:snRNP protein component of spliceosomal snRNPs, required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed Gene:BUD8(YLR353W)|FD-Score:3.72|P-value:9.86E-5||SGD DESC:Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole Gene:CAP2(YIL034C)|FD-Score:-5.63|P-value:8.81E-9||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CBP1(YJL209W)|FD-Score:-3.58|P-value:1.71E-4||SGD DESC:Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress Gene:CGI121(YML036W)|FD-Score:-3.87|P-value:5.51E-5||SGD DESC:Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification Gene:CLN2(YPL256C)|FD-Score:4.51|P-value:3.17E-6||SGD DESC:G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p) Gene:CMC1(YKL137W)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Evolutionarily conserved copper-binding protein of the mitochondrial intermembrane space, may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif Gene:CTF8(YHR191C)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Subunit of a complex with Ctf18p that shares some subunits with Replication Factor C and is required for sister chromatid cohesion Gene:DAL5(YJR152W)|FD-Score:3.2|P-value:6.98E-4||SGD DESC:Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression Gene:DEG1(YFL001W)|FD-Score:-3.53|P-value:2.04E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at position 38 or 39 in tRNA, important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability Gene:DIC1(YLR348C)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Mitochondrial dicarboxylate carrier, integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix Gene:EFG1(YGR271C-A)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:ERS1(YCR075C)|FD-Score:-4.14|P-value:1.76E-5||SGD DESC:Protein with similarity to human cystinosin, which is a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains Gene:ERV46(YAL042W)|FD-Score:-3.92|P-value:4.46E-5||SGD DESC:Protein localized to COPII-coated vesicles, forms a complex with Erv41p; involved in the membrane fusion stage of transport Gene:FLC2(YAL053W)|FD-Score:3.58|P-value:1.71E-4||SGD DESC:Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication Gene:FPR1(YNL135C)|FD-Score:-5.59|P-value:1.14E-8||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase), binds to the drugs FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function Gene:FRE2(YKL220C)|FD-Score:5.07|P-value:2.00E-7||SGD DESC:Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels Gene:GEA2(YEL022W)|FD-Score:-3.98|P-value:3.42E-5||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication Gene:GIT1(YCR098C)|FD-Score:4.1|P-value:2.10E-5||SGD DESC:Plasma membrane permease, mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability Gene:GPM3(YOL056W)|FD-Score:-5.16|P-value:1.23E-7||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GTR2(YGR163W)|FD-Score:-5.16|P-value:1.25E-7||SGD DESC:Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD Gene:GUD1(YDL238C)|FD-Score:3.37|P-value:3.73E-4||SGD DESC:Guanine deaminase, a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures Gene:HHF1(YBR009C)|FD-Score:-3.21|P-value:6.60E-4||SGD DESC:Histone H4, core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity Gene:HOP1(YIL072W)|FD-Score:4.05|P-value:2.54E-5||SGD DESC:Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein?DNA filaments Gene:HPM1(YIL110W)|FD-Score:3.48|P-value:2.55E-4||SGD DESC:AdoMet-dependent methyltransferase involved in a novel 3-methylhistidine modification of ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance Gene:HUA1(YGR268C)|FD-Score:6.24|P-value:2.12E-10||SGD DESC:Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:ICS2(YBR157C)|FD-Score:3.26|P-value:5.50E-4||SGD DESC:Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization Gene:IFA38(YBR159W)|FD-Score:5.57|P-value:1.24E-8||SGD DESC:Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides Gene:IMG2(YCR071C)|FD-Score:3.94|P-value:4.11E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:IML3(YBR107C)|FD-Score:4.41|P-value:5.08E-6||SGD DESC:Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1 Gene:IRC11(YOR013W_d)|FD-Score:5.59|P-value:1.16E-8||SGD DESC:Dubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRS4(YKR019C)|FD-Score:-4.24|P-value:1.10E-5||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LAT1(YNL071W)|FD-Score:3.46|P-value:2.72E-4||SGD DESC:Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA Gene:LEM3(YNL323W)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane Gene:LRE1(YCL051W)|FD-Score:-3.8|P-value:7.23E-5||SGD DESC:Protein involved in control of cell wall structure and stress response; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; <br>LRE1 has a paralog, HLR1, that arose from the whole genome duplication Gene:MCX1(YBR227C)|FD-Score:-3.14|P-value:8.56E-4||SGD DESC:Mitochondrial matrix protein; putative ATP-binding chaperone with non-proteolytic function; similar to bacterial ClpX proteins Gene:MDJ2(YNL328C)|FD-Score:-4.8|P-value:7.94E-7||SGD DESC:Constituent of the mitochondrial import motor associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain Gene:MNL2(YLR057W)|FD-Score:3.12|P-value:9.09E-4||SGD DESC:Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene Gene:MNS1(YJR131W)|FD-Score:6.53|P-value:3.20E-11||SGD DESC:Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation Gene:MOH1(YBL049W)|FD-Score:5.73|P-value:4.95E-9||SGD DESC:Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase Gene:MRM1(YOR201C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Ribose methyltransferase that modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA Gene:MRP13(YGR084C)|FD-Score:3.12|P-value:9.02E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRPL16(YBL038W)|FD-Score:4.74|P-value:1.07E-6||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL37(YBR268W)|FD-Score:3.75|P-value:8.93E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL38(YKL170W)|FD-Score:-3.33|P-value:4.31E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPL39(YML009C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSA2(YKR077W)|FD-Score:4.84|P-value:6.48E-7||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MSB3(YNL293W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:Rab GTPase-activating protein; regulates endocytosis via activation of Vps21p and Ypt7p; also acts on Ypt52p and Sec4p; also required for proper actin organization; localizes to the plasma membrane and sites of polarized growth, and a portion localizes to endosomes and vacuoles; similar to Msb4p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:MSN2(YMR037C)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:MST27(YGL051W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles Gene:PDR1(YGL013C)|FD-Score:-4.91|P-value:4.49E-7||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PDR8(YLR266C)|FD-Score:-3.42|P-value:3.14E-4||SGD DESC:Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication Gene:PGD1(YGL025C)|FD-Score:-4.88|P-value:5.34E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor Gene:POR2(YIL114C)|FD-Score:4.33|P-value:7.33E-6||SGD DESC:Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication Gene:PUS6(YGR169C)|FD-Score:4.26|P-value:1.02E-5||SGD DESC:tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability Gene:PXL1(YKR090W)|FD-Score:6.06|P-value:6.92E-10||SGD DESC:Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:RAD23(YEL037C)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover Gene:RFX1(YLR176C)|FD-Score:4.07|P-value:2.37E-5||SGD DESC:Major transcriptional repressor of DNA-damage-regulated genes, recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins Gene:RMD8(YFR048W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Cytosolic protein required for sporulation Gene:RMD9(YGL107C)|FD-Score:-3.22|P-value:6.39E-4||SGD DESC:Mitochondrial protein required for respiratory growth; mutant phenotype and genetic interactions suggest a role in delivering mt mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes Gene:RPL13A(YDL082W)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication Gene:RPL1B(YGL135W)|FD-Score:-3.27|P-value:5.46E-4||SGD DESC:Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal Gene:RPL35A(YDL191W)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication Gene:RPS4A(YJR145C)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication Gene:RTG3(YBL103C)|FD-Score:3.94|P-value:4.08E-5||SGD DESC:Basic helix-loop-helix-leucine zipper (bHLH/Zip) transcription factor that forms a complex with another bHLH/Zip protein, Rtg1p, to activate the retrograde (RTG) and TOR pathways Gene:SER2(YGR208W)|FD-Score:4.43|P-value:4.65E-6||SGD DESC:Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source Gene:SER33(YIL074C)|FD-Score:6.42|P-value:6.79E-11||SGD DESC:3-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication Gene:SHS1(YDL225W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SIT4(YDL047W)|FD-Score:-3.88|P-value:5.31E-5||SGD DESC:Type 2A-related serine-threonine phosphatase that functions in the G1/S transition of the mitotic cycle; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization Gene:SLG1(YOR008C)|FD-Score:3.13|P-value:8.81E-4||SGD DESC:Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response Gene:SPA2(YLL021W)|FD-Score:-3.54|P-value:1.98E-4||SGD DESC:Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate Gene:SPH1(YLR313C)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein involved in shmoo formation and bipolar bud site selection; homologous to Spa2p, localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p Gene:SSK1(YLR006C)|FD-Score:-4.86|P-value:5.87E-7||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SWF1(YDR126W)|FD-Score:4.25|P-value:1.07E-5||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SWR1(YDR334W)|FD-Score:4.4|P-value:5.41E-6||SGD DESC:Swi2/Snf2-related ATPase that is the structural component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A Gene:SYN8(YAL014C)|FD-Score:-3.53|P-value:2.08E-4||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TED1(YIL039W)|FD-Score:3.23|P-value:6.12E-4||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:THI72(YOR192C)|FD-Score:-3.69|P-value:1.10E-4||SGD DESC:Transporter of thiamine or related compound; shares sequence similarity with Thi7p Gene:TMA16(YOR252W)|FD-Score:4.86|P-value:5.98E-7||SGD DESC:Protein of unknown function that associates with ribosomes Gene:UGA2(YBR006W)|FD-Score:-3.69|P-value:1.11E-4||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:UPS3(YDR185C)|FD-Score:4.61|P-value:2.02E-6||SGD DESC:Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations Gene:URE2(YNL229C)|FD-Score:3.82|P-value:6.63E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VID28(YIL017C)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPH1(YOR270C)|FD-Score:3.67|P-value:1.20E-4||SGD DESC:Subunit a of vacuolar-ATPase V0 domain; one of two isoforms (Vph1p and Stv1p); Vph1p is located in V-ATPase complexes of the vacuole while Stv1p is located in V-ATPase complexes of the Golgi and endosomes; relative distribution to the vacuolar membrane decreases upon DNA replication stress Gene:VPS55(YJR044C)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YBL039W-B(YBL039W-B_p)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:Putative protein of unknown function Gene:YCL012C(YCL012C)|FD-Score:-3.81|P-value:6.82E-5||SGD DESC:Putative protein of unknown function; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene Gene:YCR051W(YCR051W_p)|FD-Score:-4.2|P-value:1.32E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene Gene:YCR085W(YCR085W_d)|FD-Score:-4.37|P-value:6.28E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR149C(YDR149C_d)|FD-Score:-3.72|P-value:9.81E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1; null mutation blocks anaerobic growth Gene:YDR186C(YDR186C)|FD-Score:-3.51|P-value:2.27E-4||SGD DESC:Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YDR433W(YDR433W_d)|FD-Score:3.92|P-value:4.35E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YER186C(YER186C_p)|FD-Score:-5.8|P-value:3.30E-9||SGD DESC:Putative protein of unknown function Gene:YGR050C(YGR050C_p)|FD-Score:8.76|P-value:9.85E-19||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YIL067C(YIL067C_p)|FD-Score:3.47|P-value:2.58E-4||SGD DESC:Uncharacterized protein of unknown function Gene:YKR018C(YKR018C_p)|FD-Score:3.95|P-value:3.86E-5||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication Gene:YLL059C(YLL059C_d)|FD-Score:-6.88|P-value:2.92E-12||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR031W(YLR031W_p)|FD-Score:3.18|P-value:7.31E-4||SGD DESC:Putative protein of unknown function Gene:YLR285C-A(YLR285C-A_p)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YNL319W(YNL319W_d)|FD-Score:3.84|P-value:6.18E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HXT14 Gene:YOL107W(YOL107W_p)|FD-Score:-3.43|P-value:3.03E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR342C(YOR342C_p)|FD-Score:-3.55|P-value:1.91E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:-4.39|P-value:5.66E-6||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPL039W(YPL039W_p)|FD-Score:-3.43|P-value:2.98E-4||SGD DESC:Putative protein of unknown function; YPL039W is not an essential gene Gene:YPL182C(YPL182C_d)|FD-Score:5.29|P-value:6.03E-8||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W Gene:YPR146C(YPR146C_d)|FD-Score:3.36|P-value:3.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YPR133C8.392.35E-172.68SPN1Protein involved in RNA polymerase II transcription; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
YBR002C5.715.63E-90.12RER2Cis-prenyltransferase involved in dolichol synthesis; participates in endoplasmic reticulum (ER) protein sorting
YKL083W_d5.601.10E-80.54YKL083W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
YMR185W_p5.062.11E-70.79RTP1_pProtein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
YCL043C4.279.79E-60.07PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
YLR076C_d4.201.34E-50.05YLR076C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
YDR045C4.151.68E-50.30RPC11RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
YNL216W3.855.90E-50.31RAP1Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
YMR240C3.542.02E-40.01CUS1Protein required for assembly of U2 snRNP into the spliceosome, forms a complex with Hsh49p and Hsh155p
YPL075W3.522.14E-40.06GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p
YPR034W3.472.63E-40.05ARP7Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YER013W3.413.20E-40.02PRP22DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
YHR023W3.403.38E-40.01MYO1Type II myosin heavy chain, required for wild-type cytokinesis and cell separation; localizes to the actomyosin ring; binds to myosin light chains Mlc1p and Mlc2p through its IQ1 and IQ2 motifs respectively
YPL122C3.393.48E-40.09TFB2Subunit of TFIIH and nucleotide excision repair factor 3 complexes, involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
YFL024C3.304.80E-40.05EPL1Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR050C_p8.769.85E-19YGR050C_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YBR149W6.757.50E-12ARA1NADP+ dependent arabinose dehydrogenase, involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
YJR131W6.533.20E-11MNS1Alpha-1,2-mannosidase involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
YIL074C6.426.79E-11SER333-phosphoglycerate dehydrogenase; catalyzes the first step in serine and glycine biosynthesis; SER33 has a paralog, SER3, that arose from the whole genome duplication
YGR268C6.242.12E-10HUA1Cytoplasmic protein containing a zinc finger domain with sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
YKR090W6.066.92E-10PXL1Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
YBL049W5.734.95E-9MOH1Protein of unknown function, has homology to kinase Snf7p; not required for growth on nonfermentable carbon sources; essential for survival in stationary phase
YOR013W_d5.591.16E-8IRC11_dDubious opening reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012C; null mutant displays increased levels of spontaneous Rad52 foci
YBR159W5.571.24E-8IFA38Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
YPL182C_d5.296.03E-8YPL182C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
YBL039W-B_p5.151.31E-7YBL039W-B_pPutative protein of unknown function
YKL220C5.072.00E-7FRE2Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
YOR252W4.865.98E-7TMA16Protein of unknown function that associates with ribosomes
YKR077W4.846.48E-7MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YLR227C4.788.96E-7ADY4Structural component of the meiotic outer plaque, which is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane

GO enrichment analysis for SGTC_2337
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0912.67E-12SGTC_2234tolnaftate 200.0 μMMiscellaneous55100.180328
0.0864.29E-11SGTC_23317980721 200.0 μMChembridge (Fragment library)29812210.0757576
0.0631.38E-6SGTC_23349017922 200.0 μMChembridge (Fragment library)31631640.0704225
0.0621.73E-6SGTC_1742st038235 28.9 μMTimTec (Natural product derivative library)5641030.106667mitochondrial stress
0.0612.26E-6SGTC_2513baicalein 25.6 μMMicrosource (Natural product library)52816050.0967742calcium & mitochondrial duress
0.0571.32E-5SGTC_100279-0212 42.6 μMChemDiv (Drug-like library)398510.0526316TRP & mitochondrial translation
0.0525.56E-5SGTC_33159136395 68.5 μMChembridge (Drug-like library)168768440.101266
0.0526.49E-5SGTC_13461488-2224 62.4 μMChemDiv (Drug-like library)37224270.0588235
0.0517.71E-5SGTC_6304356-0227 183.0 μMChemDiv (Drug-like library)7793120.109375
0.0501.07E-4SGTC_1695st026914 34.5 μMTimTec (Natural product derivative library)32891910.118421
0.0501.15E-4SGTC_1108betulinic acid 729.1 nMTimTec (Natural product library)649450.1
0.0501.35E-4SGTC_540159-0055 9.0 μMChemDiv (Drug-like library)33190990.0704225ERG2
0.0491.89E-4SGTC_2759disulfiram 3.4 μMMiscellaneous31170.02
0.0473.00E-4SGTC_2709st078560 56.0 μMTimTec (Natural product derivative library)172509030.0987654
0.0473.10E-4SGTC_223busulfan 5.0 mMMiscellaneous24780.0204082

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22947971431200 μM0.5454555741572Chembridge (Fragment library)221.31861.52313
SGTC_245553777129.75 μM0.342243908Miscellaneous219.259662.42813
SGTC_21906049624200 μM0.2592592265554Chembridge (Fragment library)229.27441.86313
SGTC_21896047310154.43 μM0.245614711535Chembridge (Fragment library)243.300982.08903RPP1 & pyrimidine depletion
SGTC_1881565248416 μM0.2352942255129Miscellaneous417.319485.30814TRP & mitochondrial translation
SGTC_3049909222349.47 μM0.2258068623917Chembridge (Drug-like library)328.835843.93502
SGTC_13853091-483414.1 μM0.2241384072814ChemDiv (Drug-like library)252.267982.99213RPP1 & pyrimidine depletion
SGTC_2880904298358.44 μM0.218756464714Chembridge (Drug-like library)341.4224633.18914fatty acid desaturase (OLE1)
SGTC_840443-0269128.82 μM0.2166676150058ChemDiv (Drug-like library)266.294564.16703
SGTC_3051909377849.47 μM0.21538517217378Chembridge (Drug-like library)338.443343.5903