7987427

1-(2-methoxyethyl)-3-(3-methylpyridin-2-yl)thiourea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2340
Screen concentration 183.4 μM
Source Chembridge (Fragment library)
PubChem CID 2239331
SMILES CC1=C(N=CC=C1)NC(=S)NCCOC
Standardized SMILES COCCNC(=S)Nc1ncccc1C
Molecular weight 225.3106
ALogP 2.26
H-bond donor count 2
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 13.71
% growth inhibition (Hom. pool) 7.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 2239331
Download HIP data (tab-delimited text)  (excel)
Gene:APC5(YOR249C)|FD-Score:-4.15|P-value:1.67E-5|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC27(YBL084C)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.48||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CWC22(YGR278W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DHR2(YKL078W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.01||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ERG11(YHR007C)|FD-Score:6.77|P-value:6.54E-12|Clearance:1.5||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HEM4(YOR278W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MGE1(YOR232W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.14||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.06||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUS1(YDL193W)|FD-Score:4.28|P-value:9.51E-6|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POB3(YML069W)|FD-Score:5.27|P-value:6.91E-8|Clearance:0.15||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PUP1(YOR157C)|FD-Score:5.11|P-value:1.58E-7|Clearance:0.21||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:SEC53(YFL045C)|FD-Score:4.9|P-value:4.81E-7|Clearance:0.62||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SRP14(YDL092W)|FD-Score:-3.97|P-value:3.60E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TIF34(YMR146C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.08||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM44(YIL022W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.36||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:APC5(YOR249C)|FD-Score:-4.15|P-value:1.67E-5|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress Gene:CDC27(YBL084C)|FD-Score:4.17|P-value:1.49E-5|Clearance:0.48||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition Gene:CWC22(YGR278W)|FD-Score:-4.18|P-value:1.48E-5|Clearance:0||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DHR2(YKL078W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.01||SGD DESC:Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis Gene:ERG11(YHR007C)|FD-Score:6.77|P-value:6.54E-12|Clearance:1.5||SGD DESC:Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p Gene:HEM4(YOR278W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria Gene:MGE1(YOR232W)|FD-Score:3.7|P-value:1.09E-4|Clearance:0.14||SGD DESC:Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE Gene:NIP1(YMR309C)|FD-Score:3.11|P-value:9.51E-4|Clearance:0.06||SGD DESC:eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection Gene:NUS1(YDL193W)|FD-Score:4.28|P-value:9.51E-6|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:POB3(YML069W)|FD-Score:5.27|P-value:6.91E-8|Clearance:0.15||SGD DESC:Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress Gene:PUP1(YOR157C)|FD-Score:5.11|P-value:1.58E-7|Clearance:0.21||SGD DESC:Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z Gene:SEC53(YFL045C)|FD-Score:4.9|P-value:4.81E-7|Clearance:0.62||SGD DESC:Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Gene:SRP14(YDL092W)|FD-Score:-3.97|P-value:3.60E-5|Clearance:0||SGD DESC:Signal recognition particle (SRP) subunit, interacts with the RNA component of SRP to form the Alu domain, which is the region of SRP responsible for arrest of nascent chain elongation during membrane targeting; homolog of mammalian SRP14 Gene:TIF34(YMR146C)|FD-Score:3.18|P-value:7.24E-4|Clearance:0.08||SGD DESC:eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation Gene:TIM44(YIL022W)|FD-Score:3.54|P-value:1.97E-4|Clearance:0.36||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 2239331
Download HOP data (tab-delimited text)  (excel)
Gene:AIM11(YER093C-A)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALD2(YMR170C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APE1(YKL103C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:ARO3(YDR035W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG17(YLR423C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG23(YLR431C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG36(YJL185C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT2(YEL064C)|FD-Score:-3.79|P-value:7.48E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCH1(YMR237W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CAF40(YNL288W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCW12(YLR110C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS6(YJL099W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CUP2(YGL166W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DDC1(YPL194W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DGR1(YNL130C-A_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DSD1(YGL196W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ERP1(YAR002C-A)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:5.29|P-value:6.00E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP43(YGR243W)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FRE4(YNR060W)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE8(YLR047C)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GCN4(YEL009C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA1(YDR358W)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GSM1(YJL103C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GTR1(YML121W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HEL1(YKR017C_p)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HIS4(YCL030C)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HOM2(YDR158W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSL1(YKL101W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXT3(YDR345C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IDP3(YNL009W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IRC14(YOR135C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC15(YPL017C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:IRC4(YDR540C)|FD-Score:-4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:6.93|P-value:2.17E-12||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIP2(YPL155C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MDS3(YGL197W)|FD-Score:5.76|P-value:4.17E-9||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MSW1(YDR268W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUD1(YBR119W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP1(YGR055W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT1(YDL040C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NGG1(YDR176W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NHP10(YDL002C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA111(YNL123W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPR2(YEL062W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:PDB1(YBR221C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR1(YGL013C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP4(YPL154C)|FD-Score:5.76|P-value:4.31E-9||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHO23(YNL097C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PHO87(YCR037C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:POR1(YNL055C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PPZ2(YDR436W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRO2(YOR323C)|FD-Score:6.16|P-value:3.65E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC3(YBL056W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:PTC6(YCR079W)|FD-Score:-5.4|P-value:3.25E-8||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RHR2(YIL053W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL17B(YJL177W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17B(YDR447C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT14(YIL127C_p)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RSB1(YOR049C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SIZ1(YDR409W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLM4(YBR077C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SSO1(YPL232W)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STO1(YMR125W)|FD-Score:7.43|P-value:5.30E-14||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TAN1(YGL232W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA10(YGR205W)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TIR1(YER011W)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TMA46(YOR091W)|FD-Score:6.18|P-value:3.25E-10||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOM70(YNL121C)|FD-Score:4.81|P-value:7.56E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VMA1(YDL185W)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMR1(YHL035C)|FD-Score:-4.3|P-value:8.69E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:WHI3(YNL197C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:XYL2(YLR070C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YDR008C(YDR008C_d)|FD-Score:9.77|P-value:7.42E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:Putative kinase Gene:YDR133C(YDR133C_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR535C(YDR535C_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEH2(YLR020C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFR020W(YFR020W_p)|FD-Score:6.73|P-value:8.72E-12||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL088W(YGL088W_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL117W(YGL117W_p)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Putative protein of unknown function Gene:YIL165C(YIL165C_p)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKL222C(YKL222C)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKU70(YMR284W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR111W(YLR111W_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR122C(YLR122C_d)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR407W(YLR407W_p)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR018W(YMR018W_p)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function Gene:YOR152C(YOR152C_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR289W(YOR289W_p)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL225W(YPL225W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPQ1(YOL092W_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR003C(YPR003C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSR3(YKR053C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:YTA6(YPL074W)|FD-Score:7.11|P-value:5.96E-13||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress Gene:AIM11(YER093C-A)|FD-Score:4.58|P-value:2.31E-6||SGD DESC:Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication Gene:ALD2(YMR170C)|FD-Score:3.96|P-value:3.72E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:APE1(YKL103C)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress Gene:ARO3(YDR035W)|FD-Score:5.15|P-value:1.31E-7||SGD DESC:3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan Gene:ATG17(YLR423C)|FD-Score:3.42|P-value:3.15E-4||SGD DESC:Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells Gene:ATG23(YLR431C)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:Peripheral membrane protein required for the cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p Gene:ATG36(YJL185C)|FD-Score:-3.9|P-value:4.89E-5||SGD DESC:Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene Gene:AVT2(YEL064C)|FD-Score:-3.79|P-value:7.48E-5||SGD DESC:Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:BCH1(YMR237W)|FD-Score:3.86|P-value:5.58E-5||SGD DESC:Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress; BCH1 has a paralog, BUD7, that arose from the whole genome duplication Gene:BDF1(YLR399C)|FD-Score:-3.21|P-value:6.73E-4||SGD DESC:Protein involved in transcription initiation at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf2p Gene:CAF40(YNL288W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p Gene:CCW12(YLR110C)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS6(YJL099W)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication Gene:CLU1(YMR012W)|FD-Score:3.5|P-value:2.37E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:CUP2(YGL166W)|FD-Score:3.13|P-value:8.85E-4||SGD DESC:Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication Gene:DDC1(YPL194W)|FD-Score:-3.42|P-value:3.17E-4||SGD DESC:DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress Gene:DFG5(YMR238W)|FD-Score:3.78|P-value:7.72E-5||SGD DESC:Putative mannosidase, essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p Gene:DGR1(YNL130C-A_p)|FD-Score:3.1|P-value:9.57E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DSD1(YGL196W)|FD-Score:4.36|P-value:6.53E-6||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:ERP1(YAR002C-A)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FET4(YMR319C)|FD-Score:5.29|P-value:6.00E-8||SGD DESC:Low-affinity Fe(II) transporter of the plasma membrane Gene:FMP43(YGR243W)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress Gene:FRE4(YNR060W)|FD-Score:-5.16|P-value:1.24E-7||SGD DESC:Ferric reductase, reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FRE8(YLR047C)|FD-Score:-3.35|P-value:3.98E-4||SGD DESC:Protein with sequence similarity to iron/copper reductases, involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p Gene:GCN4(YEL009C)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:GGA1(YDR358W)|FD-Score:-4.13|P-value:1.78E-5||SGD DESC:Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi Gene:GSM1(YJL103C)|FD-Score:-3.17|P-value:7.75E-4||SGD DESC:Putative zinc cluster protein of unknown function; proposed to be involved in the regulation of energy metabolism, based on patterns of expression and sequence analysis Gene:GTR1(YML121W)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB Gene:HEL1(YKR017C_p)|FD-Score:-3.77|P-value:8.13E-5||SGD DESC:RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU) Gene:HIS4(YCL030C)|FD-Score:3.82|P-value:6.57E-5||SGD DESC:Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase, phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis Gene:HOM2(YDR158W)|FD-Score:4.12|P-value:1.93E-5||SGD DESC:Aspartic beta semi-aldehyde dehydrogenase, catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis Gene:HSL1(YKL101W)|FD-Score:4.26|P-value:1.04E-5||SGD DESC:Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates with the assembled septin filament; required along with Hsl7p for bud neck recruitment, phosphorylation, and degradation of Swe1p Gene:HXT3(YDR345C)|FD-Score:4.62|P-value:1.95E-6||SGD DESC:Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication Gene:IDP3(YNL009W)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication Gene:IRC14(YOR135C_d)|FD-Score:4.24|P-value:1.10E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci Gene:IRC15(YPL017C)|FD-Score:4.68|P-value:1.41E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:IRC4(YDR540C)|FD-Score:-4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:6.93|P-value:2.17E-12||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:KIP2(YPL155C)|FD-Score:3.15|P-value:8.07E-4||SGD DESC:Kinesin-related motor protein involved in mitotic spindle positioning, stabilizes microtubules by targeting Bik1p to the plus end; Kip2p levels are controlled during the cell cycle Gene:MDS3(YGL197W)|FD-Score:5.76|P-value:4.17E-9||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MSW1(YDR268W)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:MUD1(YBR119W)|FD-Score:-3.17|P-value:7.68E-4||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP1(YGR055W)|FD-Score:3.96|P-value:3.76E-5||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:NAT1(YDL040C)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Subunit of the N-terminal acetyltransferase NatA (Nat1p, Ard1p, Nat5p); N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing Gene:NGG1(YDR176W)|FD-Score:-3.1|P-value:9.75E-4||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NHP10(YDL002C)|FD-Score:3.15|P-value:8.20E-4||SGD DESC:Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair Gene:NMA111(YNL123W)|FD-Score:3.91|P-value:4.66E-5||SGD DESC:Serine protease and general molecular chaperone; involved in response to heat stress and promotion of apoptosis; may contribute to lipid homeostasis; sequence similarity to the mammalian Omi/HtrA2 family of serine proteases Gene:NPR2(YEL062W)|FD-Score:3.69|P-value:1.13E-4||SGD DESC:Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline Gene:PDB1(YBR221C)|FD-Score:3.75|P-value:8.73E-5||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR1(YGL013C)|FD-Score:-4.22|P-value:1.24E-5||SGD DESC:Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication Gene:PEP4(YPL154C)|FD-Score:5.76|P-value:4.31E-9||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PHO23(YNL097C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Probable component of the Rpd3 histone deacetylase complex, involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus has similarity to human candidate tumor suppressor p33(ING1) and its isoform ING3 Gene:PHO87(YCR037C)|FD-Score:3.61|P-value:1.56E-4||SGD DESC:Low-affinity inorganic phosphate (Pi) transporter; involved in activation of PHO pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:4.68|P-value:1.45E-6||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:POR1(YNL055C)|FD-Score:-3.49|P-value:2.38E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PPZ2(YDR436W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:PRO2(YOR323C)|FD-Score:6.16|P-value:3.65E-10||SGD DESC:Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis Gene:PTC3(YBL056W)|FD-Score:3.78|P-value:7.89E-5||SGD DESC:Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication Gene:PTC6(YCR079W)|FD-Score:-5.4|P-value:3.25E-8||SGD DESC:Mitochondrial type 2C protein phosphatase (PP2C) with similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase Gene:RHR2(YIL053W)|FD-Score:3.33|P-value:4.42E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RNP1(YLL046C)|FD-Score:3.13|P-value:8.64E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:RPL17B(YJL177W)|FD-Score:3.33|P-value:4.38E-4||SGD DESC:Ribosomal 60S subunit protein L17B; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication Gene:RPL40A(YIL148W)|FD-Score:-3.45|P-value:2.77E-4||SGD DESC:Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:RPS17B(YDR447C)|FD-Score:3.66|P-value:1.28E-4||SGD DESC:Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RRT14(YIL127C_p)|FD-Score:4.83|P-value:6.77E-7||SGD DESC:Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis Gene:RSB1(YOR049C)|FD-Score:3.72|P-value:9.83E-5||SGD DESC:Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane Gene:SIZ1(YDR409W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLM4(YBR077C)|FD-Score:-4.11|P-value:1.99E-5||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4 Gene:SSO1(YPL232W)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STO1(YMR125W)|FD-Score:7.43|P-value:5.30E-14||SGD DESC:Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80 Gene:TAN1(YGL232W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress Gene:TDA10(YGR205W)|FD-Score:-3.78|P-value:7.95E-5||SGD DESC:ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele Gene:TIR1(YER011W)|FD-Score:-3.15|P-value:8.26E-4||SGD DESC:Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking Gene:TMA46(YOR091W)|FD-Score:6.18|P-value:3.25E-10||SGD DESC:Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p Gene:TOM70(YNL121C)|FD-Score:4.81|P-value:7.56E-7||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.58|P-value:1.69E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:VMA1(YDL185W)|FD-Score:5.04|P-value:2.33E-7||SGD DESC:Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease Gene:VMR1(YHL035C)|FD-Score:-4.3|P-value:8.69E-6||SGD DESC:Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions Gene:WHI3(YNL197C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:RNA binding protein that sequesters CLN3 mRNA in cytoplasmic foci;; Tpk1 (PKA) mediated phosphorylation of Whi3 affects G1/S transition by modulating Cln3 transcription; cytoplasmic retention factor for Cdc28p and associated cyclins; regulates cell fate and dose-dependently regulates the critical cell size required for passage through Start; WHI3 has a paralog, WHI4, that arose from the whole genome duplication Gene:XYL2(YLR070C)|FD-Score:-3.46|P-value:2.69E-4||SGD DESC:Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect Gene:YDR008C(YDR008C_d)|FD-Score:9.77|P-value:7.42E-23||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR109C(YDR109C_p)|FD-Score:5.68|P-value:6.88E-9||SGD DESC:Putative kinase Gene:YDR133C(YDR133C_d)|FD-Score:-3.3|P-value:4.90E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C Gene:YDR535C(YDR535C_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene. Gene:YEH2(YLR020C)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism Gene:YFR020W(YFR020W_p)|FD-Score:6.73|P-value:8.72E-12||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data Gene:YGL088W(YGL088W_d)|FD-Score:3.11|P-value:9.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YGL117W(YGL117W_p)|FD-Score:4.04|P-value:2.67E-5||SGD DESC:Putative protein of unknown function Gene:YIL165C(YIL165C_p)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene Gene:YKL222C(YKL222C)|FD-Score:-3.89|P-value:5.01E-5||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YKU70(YMR284W)|FD-Score:3.2|P-value:6.89E-4||SGD DESC:Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair Gene:YLR111W(YLR111W_d)|FD-Score:3.65|P-value:1.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR122C(YLR122C_d)|FD-Score:-3.13|P-value:8.87E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR123C Gene:YLR407W(YLR407W_p)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus Gene:YMR018W(YMR018W_p)|FD-Score:4.03|P-value:2.74E-5||SGD DESC:Putative protein of unknown function with similarity to human PEX5Rp (peroxin protein 5 related protein); transcription increases during colony development similar to genes involved in peroxisome biogenesis; YMR018W is not an essential gene Gene:YMR166C(YMR166C_p)|FD-Score:3.1|P-value:9.53E-4||SGD DESC:Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene Gene:YMR320W(YMR320W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YNL042W-B(YNL042W-B_p)|FD-Score:3.42|P-value:3.18E-4||SGD DESC:Putative protein of unknown function Gene:YOR152C(YOR152C_p)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Putative protein of unknown function; YOR152C is not an essential gene Gene:YOR289W(YOR289W_p)|FD-Score:4.25|P-value:1.09E-5||SGD DESC:Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:YPL225W(YPL225W)|FD-Score:3.64|P-value:1.38E-4||SGD DESC:Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress Gene:YPQ1(YOL092W_p)|FD-Score:-3.18|P-value:7.33E-4||SGD DESC:Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter Gene:YPR003C(YPR003C_p)|FD-Score:4.02|P-value:2.88E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR123C(YPR123C_d)|FD-Score:-3.28|P-value:5.11E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:YSR3(YKR053C)|FD-Score:3.78|P-value:7.69E-5||SGD DESC:Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication Gene:YTA6(YPL074W)|FD-Score:7.11|P-value:5.96E-13||SGD DESC:Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR007C6.776.54E-121.50ERG11Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
YML069W5.276.91E-80.15POB3Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
YOR157C5.111.58E-70.21PUP1Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
YFL045C4.904.81E-70.62SEC53Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen
YDL193W4.289.51E-60.10NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YBL084C4.171.49E-50.48CDC27Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
YOR232W3.701.09E-40.14MGE1Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
YKL078W3.561.86E-40.01DHR2Predominantly nucleolar DEAH-box ATP-dependent RNA helicase, required for 18S rRNA synthesis
YIL022W3.541.97E-40.36TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YMR146C3.187.24E-40.08TIF34eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
YMR309C3.119.51E-40.06NIP1eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3), involved in the assembly of preinitiation complex and start codon selection
YOR218C_d3.050.001150.01YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YGL073W3.040.001190.07HSF1Trimeric heat shock transcription factor, activates multiple genes in response to stresses that include hyperthermia; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; posttranslationally regulated
YGL155W2.960.001530.01CDC43Beta subunit of geranylgeranyltransferase type I, catalyzes geranylgeranylation to the cysteine residue in proteins containing a C-terminal CaaX sequence ending in Leu or Phe; has substrates important for morphogenesis
YFR050C2.950.001600.04PRE4Beta 7 subunit of the 20S proteasome

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d9.777.42E-23YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YMR125W7.435.30E-14STO1Large subunit of the nuclear mRNA cap-binding protein complex, interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
YPL074W7.115.96E-13YTA6Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
YKR019C6.932.17E-12IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YFR020W_p6.738.72E-12YFR020W_pProtein of unknown function; mRNA identified as translated by ribosome profiling data
YOR091W6.183.25E-10TMA46Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
YOR323C6.163.65E-10PRO2Gamma-glutamyl phosphate reductase, catalyzes the second step in proline biosynthesis
YGL197W5.764.17E-9MDS3Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain
YPL154C5.764.31E-9PEP4Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates
YDR109C_p5.686.88E-9YDR109C_pPutative kinase
YMR319C5.296.00E-8FET4Low-affinity Fe(II) transporter of the plasma membrane
YDR035W5.151.31E-7ARO33-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
YGR243W5.121.51E-7FMP43Highly conserved subunit of the mitochondrial pyruvate carrier; a mitochondrial inner membrane complex comprised of Fmp37p/Mpc1p and either Mpc2p or Fmp43p/Mpc3p mediates mitochondrial pyruvate uptake; more highly expressed in glucose-containing minimal medium than in lactate-containing medium; expression regulated by osmotic and alkaline stresses; protein abundance increases in response to DNA replication stress
YDL185W5.042.33E-7VMA1Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease
YIL127C_p4.836.77E-7RRT14_pPutative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis

GO enrichment analysis for SGTC_2340
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1399.02E-27SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0441176
0.1224.35E-21SGTC_8340312-0008 75.7 μMChemDiv (Drug-like library)63855730.107143NEO1
0.1225.21E-21SGTC_2851486-1325 46.0 μMChemDiv (Drug-like library)223102300.115942
0.1195.72E-20SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.12
0.1158.11E-19SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.084507
0.1132.35E-18SGTC_2669eugenol 100.0 μMMicrosource (Natural product library)33140.122807
0.1127.14E-18SGTC_1090132-0036 33.7 μMChemDiv (Drug-like library)672740.16129
0.1111.60E-17SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0645161
0.1095.04E-17SGTC_2051486-1330 71.9 μMChemDiv (Drug-like library)41188340.125
0.1071.44E-16SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0422535
0.1062.86E-16SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.0422535
0.1062.99E-16SGTC_10114112-3381 17.4 μMChemDiv (Drug-like library)28771390.138889
0.1063.89E-16SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.12069
0.1054.56E-16SGTC_1761486-1328 72.8 μMChemDiv (Drug-like library)223102310.1125
0.1016.06E-15SGTC_18825615643 20.0 μMMiscellaneous22530750.116883TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23327982454138.89 μM0.56252981748Chembridge (Fragment library)247.316123.15223
SGTC_3144908705749.47 μM0.38181817249618Chembridge (Drug-like library)326.432644.52113
SGTC_22717849410118.62 μM0.309091972430Chembridge (Fragment library)233.289542.66623
SGTC_14053909-789412 μM0.2931037104060ChemDiv (Drug-like library)293.362963.7111460S ribosome export
SGTC_9031134-0042114 μM0.285714701009ChemDiv (Drug-like library)229.300843.75522
SGTC_22657929201200 μM0.2678571265108Chembridge (Fragment library)222.309962.75633
SGTC_22727805186180 μM0.2631582956062Chembridge (Fragment library)268.335342.54524cell wall signaling
SGTC_23659042475200 μM0.2631586464479Chembridge (Fragment library)255.314982.56622
SGTC_14063909-790611.9 μM0.262295X1406ChemDiv (Drug-like library)307.389543.7734160S ribosome export
SGTC_7491000-0129128 μM0.259259708470ChemDiv (Drug-like library)257.310943.15423amide catabolism