7987486

1-[4-(4-amino-2-chlorophenyl)piperazin-1-yl]propan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2341
Screen concentration 176.7 μM
Source Chembridge (Fragment library)
PubChem CID 1258686
SMILES CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N)Cl
Standardized SMILES CCC(=O)N1CCN(CC1)c2ccc(N)cc2Cl
Molecular weight 267.7545
ALogP 1.79
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 12.04
% growth inhibition (Hom. pool) 7.63


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 1258686
Download HIP data (tab-delimited text)  (excel)
Gene:AOS1(YPR180W)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.33||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:APC2(YLR127C)|FD-Score:4.31|P-value:8.03E-6|Clearance:0.19||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CWC22(YGR278W)|FD-Score:4.56|P-value:2.58E-6|Clearance:0.07||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DAD2(YKR083C)|FD-Score:4.49|P-value:3.52E-6|Clearance:0.18||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:LAS1(YKR063C)|FD-Score:5.28|P-value:6.34E-8|Clearance:0.73||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:PRE4(YFR050C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.37||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPC37(YKR025W)|FD-Score:-5.73|P-value:5.03E-9|Clearance:0||SGD DESC:RNA polymerase III subunit C37 Gene:RPL10(YLR075W)|FD-Score:-3.17|P-value:7.57E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC58(YLR033W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SCC4(YER147C)|FD-Score:-3.91|P-value:4.71E-5|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SNM1(YDR478W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPC98(YNL126W)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TIM50(YPL063W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.73|P-value:9.71E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPR177C(YPR177C_d)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex Gene:AOS1(YPR180W)|FD-Score:4.05|P-value:2.53E-5|Clearance:0.33||SGD DESC:Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability Gene:APC2(YLR127C)|FD-Score:4.31|P-value:8.03E-6|Clearance:0.19||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:CWC22(YGR278W)|FD-Score:4.56|P-value:2.58E-6|Clearance:0.07||SGD DESC:Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein Gene:DAD2(YKR083C)|FD-Score:4.49|P-value:3.52E-6|Clearance:0.18||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis Gene:LAS1(YKR063C)|FD-Score:5.28|P-value:6.34E-8|Clearance:0.73||SGD DESC:Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability Gene:PRE4(YFR050C)|FD-Score:3.46|P-value:2.72E-4|Clearance:0.37||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:RPC37(YKR025W)|FD-Score:-5.73|P-value:5.03E-9|Clearance:0||SGD DESC:RNA polymerase III subunit C37 Gene:RPL10(YLR075W)|FD-Score:-3.17|P-value:7.57E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L10; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; homologous to mammalian ribosomal protein L10 and bacterial L16; protein abundance increases in response to DNA replication stress; mutations in the human ortholog are associated with development of T-cell acute lymphoblastic leukemia and similar changes in the yeast gene result in ribosome biogenesis defects Gene:RSC58(YLR033W)|FD-Score:3.52|P-value:2.17E-4|Clearance:0.06||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:SCC4(YER147C)|FD-Score:-3.91|P-value:4.71E-5|Clearance:0||SGD DESC:Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX Gene:SNM1(YDR478W)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP Gene:SPC98(YNL126W)|FD-Score:-3.17|P-value:7.55E-4|Clearance:0||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TIM50(YPL063W)|FD-Score:4.13|P-value:1.83E-5|Clearance:0.07||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel Gene:YMR290W-A(YMR290W-A_d)|FD-Score:3.73|P-value:9.71E-5|Clearance:0.21||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:YPR177C(YPR177C_d)|FD-Score:-3.95|P-value:3.96E-5|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 1258686
Download HOP data (tab-delimited text)  (excel)
Gene:ABP140(YOR239W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ACE2(YLR131C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:APL2(YKL135C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARK1(YNL020C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARX1(YDR101C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:BFA1(YJR053W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CHO1(YER026C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COX6(YHR051W)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DLT1(YMR126C_p)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DTD1(YDL219W)|FD-Score:5.66|P-value:7.69E-9||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EFM2(YBR271W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMP70(YLR083C)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENB1(YOL158C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERG5(YMR015C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:EXG2(YDR261C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FSH3(YOR280C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FYV12(YOR183W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GIM4(YEL003W)|FD-Score:4.81|P-value:7.58E-7||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GPD1(YDL022W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GVP36(YIL041W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:HEM14(YER014W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS1(YER055C)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HPR1(YDR138W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:JJJ3(YJR097W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:JSN1(YJR091C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:LCB5(YLR260W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MET12(YPL023C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MGA2(YIR033W)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MID2(YLR332W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMS1(YPR164W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MON2(YNL297C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NCA3(YJL116C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NYV1(YLR093C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OPI8(YKR035C_d)|FD-Score:-4.33|P-value:7.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PDB1(YBR221C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR11(YIL013C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PEP4(YPL154C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PMP1(YCR024C-A)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSR2(YLR019W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RLM1(YPL089C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RMD6(YEL072W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Protein required for sporulation Gene:RPL12A(YEL054C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-4.95|P-value:3.63E-7||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SAG1(YJR004C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SEA4(YBL104C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFC1(YJR095W)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SGE1(YPR198W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIP2(YGL208W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIZ1(YDR409W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLY41(YOR307C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SNZ2(YNL333W)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:STV1(YMR054W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TEC1(YBR083W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:TIM21(YGR033C)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TUF1(YOR187W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP13(YBL067C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UFD4(YKL010C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YDR008C(YDR008C_d)|FD-Score:7.6|P-value:1.44E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YHI9(YHR029C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR460C(YLR460C_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL295W(YNL295W_p)|FD-Score:4.33|P-value:7.54E-6||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YPR003C(YPR003C_p)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR153W(YPR153W)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:Putative protein of unknown function Gene:YPR195C(YPR195C_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPT10(YBR264C)|FD-Score:6.42|P-value:6.64E-11||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles Gene:ABP140(YOR239W)|FD-Score:-3.83|P-value:6.36E-5||SGD DESC:AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift Gene:ACE2(YLR131C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication Gene:APL2(YKL135C)|FD-Score:-3.2|P-value:6.91E-4||SGD DESC:Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress Gene:ARK1(YNL020C)|FD-Score:3.22|P-value:6.42E-4||SGD DESC:Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication Gene:ARX1(YDR101C)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex Gene:BFA1(YJR053W)|FD-Score:3.73|P-value:9.76E-5||SGD DESC:Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pathways that control exit from mitosis Gene:CHO1(YER026C)|FD-Score:5.19|P-value:1.07E-7||SGD DESC:Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline Gene:COX6(YHR051W)|FD-Score:4.45|P-value:4.25E-6||SGD DESC:Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels Gene:DLT1(YMR126C_p)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA) Gene:DTD1(YDL219W)|FD-Score:5.66|P-value:7.69E-9||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:EFM2(YBR271W)|FD-Score:-3.11|P-value:9.28E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translation elongation factors EF2 (Eft1p and Eft2p) and EF3A (Yef3p); belongs to the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; predicted to be involved in ribosome biogenesis Gene:EMP70(YLR083C)|FD-Score:-4.63|P-value:1.81E-6||SGD DESC:Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments Gene:ENB1(YOL158C)|FD-Score:3.32|P-value:4.58E-4||SGD DESC:Endosomal ferric enterobactin transporter, expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment Gene:ERG5(YMR015C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs Gene:EXG2(YDR261C)|FD-Score:3.22|P-value:6.37E-4||SGD DESC:Exo-1,3-beta-glucanase, involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor Gene:FSH3(YOR280C)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; sequence is similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2 Gene:FYV12(YOR183W_p)|FD-Score:3.62|P-value:1.48E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:GIM4(YEL003W)|FD-Score:4.81|P-value:7.58E-7||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GPD1(YDL022W)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; homolog of Gpd2p; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases YPK1 and YPK2, dephosphorylation increases catalytic activity Gene:GVP36(YIL041W)|FD-Score:-3.13|P-value:8.68E-4||SGD DESC:BAR domain-containing protein that localizes to both early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis Gene:HEM14(YER014W)|FD-Score:-3.58|P-value:1.72E-4||SGD DESC:Protoporphyrinogen oxidase, a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides Gene:HIS1(YER055C)|FD-Score:-3.78|P-value:7.86E-5||SGD DESC:ATP phosphoribosyltransferase, a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HPR1(YDR138W)|FD-Score:-3.44|P-value:2.93E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:JJJ3(YJR097W)|FD-Score:-3.42|P-value:3.08E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:JSN1(YJR091C)|FD-Score:3.27|P-value:5.45E-4||SGD DESC:Member of the Puf family of RNA-binding proteins, interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl Gene:LCB5(YLR260W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules Gene:MET12(YPL023C)|FD-Score:-3.57|P-value:1.78E-4||SGD DESC:Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR Gene:MGA2(YIR033W)|FD-Score:-4.2|P-value:1.35E-5||SGD DESC:ER membrane protein involved in regulation of OLE1 transcription, acts with homolog Spt23p; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting Gene:MID2(YLR332W)|FD-Score:3.52|P-value:2.12E-4||SGD DESC:O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p Gene:MMS1(YPR164W)|FD-Score:4.73|P-value:1.10E-6||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MON2(YNL297C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Peripheral membrane protein with a role in endocytosis and vacuole integrity, interacts with Arl1p and localizes to the endosome; member of the Sec7p family of proteins Gene:NCA3(YJL116C)|FD-Score:3.13|P-value:8.73E-4||SGD DESC:Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication Gene:NYV1(YLR093C)|FD-Score:5.03|P-value:2.46E-7||SGD DESC:v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane Gene:OPI8(YKR035C_d)|FD-Score:-4.33|P-value:7.33E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A Gene:PDB1(YBR221C)|FD-Score:3.21|P-value:6.73E-4||SGD DESC:E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved multi-protein complex found in mitochondria Gene:PDR11(YIL013C)|FD-Score:3.75|P-value:8.99E-5||SGD DESC:ATP-binding cassette (ABC) transporter, multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth Gene:PEP4(YPL154C)|FD-Score:4.33|P-value:7.61E-6||SGD DESC:Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates Gene:PMP1(YCR024C-A)|FD-Score:3.42|P-value:3.17E-4||SGD DESC:Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication Gene:PSR2(YLR019W)|FD-Score:3.3|P-value:4.84E-4||SGD DESC:Functionally redundant Psr1p homolog, a plasma membrane phosphatase involved in the general stress response; required with Psr1p and Whi2p for full activation of STRE-mediated gene expression, possibly through dephosphorylation of Msn2p Gene:RLM1(YPL089C)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:RMD6(YEL072W)|FD-Score:-3.13|P-value:8.86E-4||SGD DESC:Protein required for sporulation Gene:RPL12A(YEL054C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication Gene:RPL26A(YLR344W)|FD-Score:4.52|P-value:3.05E-6||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPS0A(YGR214W)|FD-Score:-4.95|P-value:3.63E-7||SGD DESC:Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication; Gene:SAG1(YJR004C)|FD-Score:3.63|P-value:1.42E-4||SGD DESC:Alpha-agglutinin of alpha-cells, binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor Gene:SEA4(YBL104C)|FD-Score:3.28|P-value:5.24E-4||SGD DESC:Subunit of the SEA (Seh1-associated) complex, a coatomer-related complex that associates dynamically with the vacuole; has an N-terminal beta-propeller fold and a C-terminal RING motif; promoter contains multiple GCN4 binding sites Gene:SFC1(YJR095W)|FD-Score:-3.77|P-value:8.29E-5||SGD DESC:Mitochondrial succinate-fumarate transporter, transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization Gene:SGE1(YPR198W)|FD-Score:3.18|P-value:7.25E-4||SGD DESC:Plasma membrane multidrug transporter of the major facilitator superfamily, acts as an extrusion permease; partial multicopy suppressor of gal11 mutations Gene:SIP2(YGL208W)|FD-Score:3.27|P-value:5.42E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SIZ1(YDR409W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring Gene:SLY41(YOR307C)|FD-Score:4.23|P-value:1.16E-5||SGD DESC:Protein involved in ER-to-Golgi transport Gene:SNZ2(YNL333W)|FD-Score:-3.77|P-value:8.02E-5||SGD DESC:Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p Gene:STV1(YMR054W)|FD-Score:3.66|P-value:1.25E-4||SGD DESC:Subunit a of the vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and Vph1p); Stv1p is located in V-ATPase complexes of the Golgi and endosomes while Vph1p is located in V-ATPase complexes of the vacuole Gene:TEC1(YBR083W)|FD-Score:3.15|P-value:8.24E-4||SGD DESC:Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member Gene:TIM21(YGR033C)|FD-Score:3.1|P-value:9.56E-4||SGD DESC:Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity Gene:TUF1(YOR187W)|FD-Score:-3.43|P-value:3.07E-4||SGD DESC:Mitochondrial translation elongation factor Tu; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans Gene:UBP13(YBL067C)|FD-Score:4.15|P-value:1.69E-5||SGD DESC:Putative ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication Gene:UFD4(YKL010C)|FD-Score:3.55|P-value:1.93E-4||SGD DESC:Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; cytoplasmic E3 involved in the degradation of ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress Gene:URA2(YJL130C)|FD-Score:3.57|P-value:1.82E-4||SGD DESC:Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP Gene:YDR008C(YDR008C_d)|FD-Score:7.6|P-value:1.44E-14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL019C(YFL019C_d)|FD-Score:3.88|P-value:5.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein; YFL019C is not an essential gene Gene:YFT2(YDR319C_p)|FD-Score:3.28|P-value:5.21E-4||SGD DESC:Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens Gene:YHI9(YHR029C)|FD-Score:3.26|P-value:5.62E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YKL096C-B(YKL096C-B_p)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YLR460C(YLR460C_p)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Member of the quinone oxidoreductase family, up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine Gene:YNL295W(YNL295W_p)|FD-Score:4.33|P-value:7.54E-6||SGD DESC:Putative protein of unknown function Gene:YOL047C(YOL047C_p)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YPR003C(YPR003C_p)|FD-Score:3.87|P-value:5.35E-5||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR153W(YPR153W)|FD-Score:6.99|P-value:1.38E-12||SGD DESC:Putative protein of unknown function Gene:YPR195C(YPR195C_d)|FD-Score:3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YPT10(YBR264C)|FD-Score:6.42|P-value:6.64E-11||SGD DESC:Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKR063C5.286.34E-80.72LAS1Protein required for pre-rRNA processing at both ends of ITS2; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S transition of the cell cycle; human ortholog is Las1L; mutants require the SSD1-v allele for viability
YGR278W4.562.58E-60.07CWC22Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
YKR083C4.493.52E-60.18DAD2Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YLR127C4.318.03E-60.19APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YPL063W4.131.83E-50.07TIM50Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); acts as receptor for the TIM23 complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
YPR180W4.052.53E-50.33AOS1Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
YMR290W-A_d3.739.71E-50.21YMR290W-A_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase
YLR033W3.522.17E-40.06RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YFR050C3.462.72E-40.37PRE4Beta 7 subunit of the 20S proteasome
YGL040C3.090.001010.18HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YIL063C2.900.001840.02YRB2Protein of unknown function involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; is not essential
YGL061C2.880.001970.02DUO1Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
YHR188C2.870.002070.01GPI16Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-Tp homolog
YDR160W2.860.002110.01SSY1Component of the SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p), which senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes
YJL203W2.850.002210.12PRP21Subunit of the SF3a splicing factor complex, required for spliceosome assembly

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR008C_d7.601.44E-14YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YPR153W6.991.38E-12YPR153WPutative protein of unknown function
YBR264C6.426.64E-11YPT10Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
YDL219W5.667.69E-9DTD1D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
YER026C5.191.07E-7CHO1Phosphatidylserine synthase, functions in phospholipid biosynthesis; catalyzes the reaction CDP-diaclyglycerol + L-serine = CMP + L-1-phosphatidylserine, transcriptionally repressed by myo-inositol and choline
YLR093C5.032.46E-7NYV1v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in the vacuolar membrane
YEL003W4.817.58E-7GIM4Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
YPR164W4.731.10E-6MMS1Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
YLR344W4.523.05E-6RPL26ARibosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
YHR051W4.454.25E-6COX6Subunit VI of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
YDR409W4.434.74E-6SIZ1SUMO/Smt3 ligase that promotes the attachment of sumo (Smt3p; small ubiquitin-related modifier) to proteins; binds Ubc9p and may bind septins; specifically required for sumoylation of septins in vivo; localized to the septin ring
YNL295W_p4.337.54E-6YNL295W_pPutative protein of unknown function
YPL154C4.337.61E-6PEP4Vacuolar aspartyl protease (proteinase A), required for the posttranslational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; synthesized as a zymogen, self-activates
YLR131C4.231.16E-5ACE2Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
YOR307C4.231.16E-5SLY41Protein involved in ER-to-Golgi transport

GO enrichment analysis for SGTC_2341
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.0863.25E-11SGTC_5423381-0244 89.3 μMChemDiv (Drug-like library)11128430.215385
0.0773.76E-9SGTC_23469031566 143.8 μMChembridge (Fragment library)47710860.176471Golgi
0.0723.11E-8SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.0724638
0.0706.17E-8SGTC_2518prenyletin 46.3 μMMicrosource (Natural product library)38734590.102941
0.0707.72E-8SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)67107480.0166667
0.0691.19E-7SGTC_23407987427 183.4 μMChembridge (Fragment library)22393310.0909091
0.0681.44E-7SGTC_2678equilin 100.0 μMMicrosource (Natural product library)32470.0972222
0.0663.91E-7SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.0923077
0.0664.34E-7SGTC_511013-0126 23.4 μMChemDiv (Drug-like library)16278500.084507
0.0655.97E-7SGTC_23459052315 157.4 μMChembridge (Fragment library)64692650.152542
0.0657.15E-7SGTC_21945927483 104.5 μMChembridge (Fragment library)7609790.160714
0.0647.58E-7SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0645161
0.0631.05E-6SGTC_500929-0078 56.4 μMChemDiv (Drug-like library)13777510.0645161
0.0631.08E-6SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.0571429
0.0631.11E-6SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.283582

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22677931562200 μM0.674419934880Chembridge (Fragment library)267.754521.79313
SGTC_2376907292281.32 μM0.54166717087968Chembridge (Fragment library)281.78112.2513
SGTC_2809796413312.99 μM0.4912281253665Chembridge (Drug-like library)420.332224.4751360S ribosome export
SGTC_2377907335162.6 μM0.447337946Chembridge (Fragment library)281.78112.25613ergosterol biosynthesis
SGTC_23849036245200 μM0.43756462766Chembridge (Fragment library)261.362621.83713
SGTC_3156909993149.47 μM0.418111053Chembridge (Drug-like library)344.835243.85903
SGTC_21856113692200 μM0.36874150Chembridge (Fragment library)250.3118632.53703ergosterol biosynthesis
SGTC_23479049783174.81 μM0.3584916467988Chembridge (Fragment library)267.754521.95413
SGTC_3029908868349.47 μM0.33928625236615Chembridge (Drug-like library)300.41853.34703
SGTC_2861902459519.48 μM0.33846216482810Chembridge (Drug-like library)401.886562.9914