0330-0087

4,4,4-trichloro-1-(4-nitrophenyl)-3-[(4-nitrophenyl)methylideneamino]butan-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_235
Screen concentration 5.0 mM
Source ChemDiv (Drug-like library)
PubChem CID 46494818
SMILES C1=CC(=CC=C1C=NC(CC(=O)C2=CC=C(C=C2)[N+](=O)[O-])C(Cl)(Cl)Cl)[N+](=O)[O-]
Standardized SMILES [O-][N+](=O)c1ccc(C=NC(CC(=O)c2ccc(cc2)[N+](=O)[O-])C(Cl)(Cl)Cl)cc1
Molecular weight 444.6533
ALogP 4.77
H-bond donor count 0
H-bond acceptor count 6
Response signature TSC3-RPN4

Pool Growth Kinetics
% growth inhibition (Het. pool) 20.08
% growth inhibition (Hom. pool) 13.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 46494818
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.08||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP9(YMR033W)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HRT1(YOL133W)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:NOP15(YNL110C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.14||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP8(YOL144W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.1||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:POP1(YNL221C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP5(YBR237W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.06||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPP1(YHR062C)|FD-Score:10|P-value:5.63E-24|Clearance:6.1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:STH1(YIL126W)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.06||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TIM22(YDL217C)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.03||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM44(YIL022W)|FD-Score:3.81|P-value:6.97E-5|Clearance:0.04||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex) Gene:ACC1(YNR016C)|FD-Score:3.43|P-value:3.03E-4|Clearance:0.08||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP9(YMR033W)|FD-Score:3.93|P-value:4.31E-5|Clearance:0.06||SGD DESC:Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation Gene:HRT1(YOL133W)|FD-Score:-3.19|P-value:7.17E-4|Clearance:0||SGD DESC:RING finger containing subunit of Skp1-Cullin-F-box ubiquitin protein ligases (SCF); required for Gic2p, Far1p, Sic1p and Cln2p degradation; may tether Cdc34p (a ubiquitin conjugating enzyme or E2) and Cdc53p (a cullin) subunits of SCF Gene:NOP15(YNL110C)|FD-Score:3.77|P-value:8.08E-5|Clearance:0.14||SGD DESC:Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm Gene:NOP8(YOL144W)|FD-Score:3.53|P-value:2.08E-4|Clearance:0.1||SGD DESC:Nucleolar protein required for 60S ribosomal subunit biogenesis Gene:POP1(YNL221C)|FD-Score:3.19|P-value:7.16E-4|Clearance:0.08||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRP5(YBR237W)|FD-Score:3.59|P-value:1.67E-4|Clearance:0.06||SGD DESC:RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA Gene:RPP1(YHR062C)|FD-Score:10|P-value:5.63E-24|Clearance:6.1||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RSC58(YLR033W)|FD-Score:3.34|P-value:4.12E-4|Clearance:0.16||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:3.63|P-value:1.40E-4|Clearance:0.04||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:STH1(YIL126W)|FD-Score:3.87|P-value:5.43E-5|Clearance:0.06||SGD DESC:ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p Gene:TIM22(YDL217C)|FD-Score:3.11|P-value:9.46E-4|Clearance:0.03||SGD DESC:Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported Gene:TIM44(YIL022W)|FD-Score:3.81|P-value:6.97E-5|Clearance:0.04||SGD DESC:Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 46494818
Download HOP data (tab-delimited text)  (excel)
Gene:AHC2(YCR082W)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:7.46|P-value:4.39E-14||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASP1(YDR321W)|FD-Score:-4.99|P-value:2.96E-7||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:AVT3(YKL146W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CTH1(YDR151C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CTR1(YPR124W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CWC21(YDR482C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:CYK3(YDL117W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:CYS3(YAL012W)|FD-Score:3.87|P-value:5.33E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FKS3(YMR306W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA1(YLL029W)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GCN20(YFR009W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GLE2(YER107C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPR1(YDL035C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GSP2(YOR185C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:ILV1(YER086W)|FD-Score:4.86|P-value:5.79E-7||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:MAC1(YMR021C)|FD-Score:8.58|P-value:4.88E-18||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MMS1(YPR164W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:NBA1(YOL070C)|FD-Score:-3.77|P-value:8.22E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:PDC1(YLR044C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PNG1(YPL096W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RPL20A(YMR242C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT103(YDR289C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RTT107(YHR154W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEG1(YMR086W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SET2(YJL168C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SLF1(YDR515W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOL2(YCR073W-A)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SUR4(YLR372W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THR1(YHR025W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRM11(YOL124C)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRM732(YMR259C_p)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRX3(YCR083W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:TSC3(YBR058C-A)|FD-Score:20.9|P-value:3.48E-97||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:URE2(YNL229C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA7(YGR020C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS28(YPL065W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS60(YDR486C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:YAR023C(YAR023C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YCR061W(YCR061W_p)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR085W(YCR085W_d)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YGR125W(YGR125W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR095W(YHR095W_d)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL029C(YIL029C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL096C(YIL096C_p)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YJL160C(YJL160C_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YKR051W(YKR051W_p)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Putative protein of unknown function Gene:YLR184W(YLR184W_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:AHC2(YCR082W)|FD-Score:5.24|P-value:8.06E-8||SGD DESC:Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex Gene:ARO1(YDR127W)|FD-Score:7.46|P-value:4.39E-14||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ASP1(YDR321W)|FD-Score:-4.99|P-value:2.96E-7||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:AVT3(YKL146W)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters Gene:CTH1(YDR151C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Member of the CCCH zinc finger family; has similarity to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; may function with Tis11p in iron homeostasis Gene:CTR1(YPR124W)|FD-Score:3.87|P-value:5.45E-5||SGD DESC:High-affinity copper transporter of the plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress Gene:CTR9(YOL145C)|FD-Score:3.45|P-value:2.75E-4||SGD DESC:Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats Gene:CWC21(YDR482C)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p Gene:CYK3(YDL117W)|FD-Score:4.52|P-value:3.11E-6||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:CYS3(YAL012W)|FD-Score:3.87|P-value:5.33E-5||SGD DESC:Cystathionine gamma-lyase, catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress Gene:DOT6(YER088C)|FD-Score:3.96|P-value:3.73E-5||SGD DESC:Protein involved in rRNA and ribosome biogenesis; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; DOT6 has a paralog, TOD6, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ERP1(YAR002C-A)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Member of the p24 family involved in ER to Golgi transport; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication Gene:FAA4(YMR246W)|FD-Score:3.71|P-value:1.06E-4||SGD DESC:Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C12:0-C16:0 chain lengths; functions in long chain fatty acid import; important for survival during stationary phase; localized to lipid particles; involved in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon DNA replication stress; FAA4 has a paralog, FAA1, that arose from the whole genome duplication Gene:FKS3(YMR306W)|FD-Score:4.01|P-value:2.99E-5||SGD DESC:Protein involved in spore wall assembly, has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA1(YLL029W)|FD-Score:3.82|P-value:6.73E-5||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation Gene:GCN20(YFR009W)|FD-Score:-3.5|P-value:2.34E-4||SGD DESC:Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA Gene:GLE2(YER107C)|FD-Score:4.18|P-value:1.48E-5||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:GOS1(YHL031C)|FD-Score:3.21|P-value:6.68E-4||SGD DESC:v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28 Gene:GPR1(YDL035C)|FD-Score:3.73|P-value:9.49E-5||SGD DESC:Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis Gene:GSP2(YOR185C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); Gsp1p homolog that is not required for viability; protein abundance increases in response to DNA replication stress Gene:HOM6(YJR139C)|FD-Score:4.09|P-value:2.20E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:ILV1(YER086W)|FD-Score:4.86|P-value:5.79E-7||SGD DESC:Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation Gene:MAC1(YMR021C)|FD-Score:8.58|P-value:4.88E-18||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:-3.45|P-value:2.83E-4||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MMS1(YPR164W)|FD-Score:3.95|P-value:3.97E-5||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:NBA1(YOL070C)|FD-Score:-3.77|P-value:8.22E-5||SGD DESC:Protein of unknown function, localizes to the bud neck and cytoplasm; interacts with Nap1p; may interact with ribosomes, based on co-purification experiments; potential Cdc28p substrate Gene:PDC1(YLR044C)|FD-Score:4.66|P-value:1.56E-6||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PEP12(YOR036W)|FD-Score:5.96|P-value:1.28E-9||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEP7(YDR323C)|FD-Score:3.77|P-value:8.26E-5||SGD DESC:Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance Gene:PNG1(YPL096W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:-3.22|P-value:6.46E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RPL20A(YMR242C)|FD-Score:3.33|P-value:4.28E-4||SGD DESC:Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication Gene:RPL42B(YHR141C)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication Gene:RPN4(YDL020C)|FD-Score:4.39|P-value:5.65E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:RTS1(YOR014W)|FD-Score:3.21|P-value:6.69E-4||SGD DESC:B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A Gene:RTT103(YDR289C)|FD-Score:3.62|P-value:1.50E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:RTT107(YHR154W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress Gene:SEG1(YMR086W)|FD-Score:3.7|P-value:1.09E-4||SGD DESC:Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation and controls eisosome length and shape; Seg1p-GFP diffusely distributed and forms heterogeneous patches at the plasma membrane in small buds, also found in medium and large buds; may interact with ribosomes, based on co-purification experiments; expression is repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes Gene:SET2(YJL168C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p Gene:SLF1(YDR515W)|FD-Score:3.12|P-value:9.06E-4||SGD DESC:RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SNF7(YLR025W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes Gene:SNF8(YPL002C)|FD-Score:3.95|P-value:3.84E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression Gene:SOL2(YCR073W-A)|FD-Score:5.18|P-value:1.11E-7||SGD DESC:Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication Gene:SOV1(YMR066W)|FD-Score:3.64|P-value:1.35E-4||SGD DESC:Mitochondrial protein of unknown function Gene:SUR4(YLR372W)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:SWC5(YBR231C)|FD-Score:3.24|P-value:5.87E-4||SGD DESC:Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress Gene:TCO89(YPL180W)|FD-Score:3.1|P-value:9.59E-4||SGD DESC:Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin Gene:THR1(YHR025W)|FD-Score:3.32|P-value:4.47E-4||SGD DESC:Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway Gene:TRM11(YOL124C)|FD-Score:-3.16|P-value:7.90E-4||SGD DESC:Catalytic subunit of an adoMet-dependent tRNA methyltransferase complex (Trm11p-Trm112p), required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; contains a THUMP domain and a methyltransferase domain Gene:TRM732(YMR259C_p)|FD-Score:-3.13|P-value:8.75E-4||SGD DESC:Interacts with Trm7p for 2'-O-methylation of C32 of substrate tRNAs; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene Gene:TRX3(YCR083W)|FD-Score:3.48|P-value:2.49E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:TSC3(YBR058C-A)|FD-Score:20.9|P-value:3.48E-97||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TUL1(YKL034W)|FD-Score:3.88|P-value:5.26E-5||SGD DESC:Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1 Gene:URE2(YNL229C)|FD-Score:3.85|P-value:5.96E-5||SGD DESC:Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion Gene:VMA7(YGR020C)|FD-Score:4.15|P-value:1.63E-5||SGD DESC:Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane Gene:VPH2(YKL119C)|FD-Score:3.85|P-value:5.95E-5||SGD DESC:Integral membrane protein required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER) Gene:VPS28(YPL065W)|FD-Score:4.55|P-value:2.67E-6||SGD DESC:Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p Gene:VPS3(YDR495C)|FD-Score:4.73|P-value:1.14E-6||SGD DESC:Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase Gene:VPS60(YDR486C)|FD-Score:3.95|P-value:3.92E-5||SGD DESC:Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p Gene:YAR023C(YAR023C_p)|FD-Score:3.93|P-value:4.26E-5||SGD DESC:Putative integral membrane protein, member of DUP240 gene family Gene:YCR061W(YCR061W_p)|FD-Score:3.12|P-value:9.17E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:YCR085W(YCR085W_d)|FD-Score:3.29|P-value:4.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR379C-A(YDR379C-A)|FD-Score:-3.82|P-value:6.71E-5||SGD DESC:Protein involved in the assembly of the mitochondrial succinate dehydrogenase complex; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy Gene:YGR125W(YGR125W_p)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Putative protein of unknown function; deletion mutant has decreased rapamycin resistance but normal wormannin resistance; green fluorescent protein (GFP)-fusion protein localizes to the vacuole Gene:YHR086W-A(YHR086W-A_p)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YHR095W(YHR095W_d)|FD-Score:-3.49|P-value:2.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIG1(YPL201C)|FD-Score:3.53|P-value:2.06E-4||SGD DESC:Protein that interacts with glycerol 3-phosphatase and plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol Gene:YIL029C(YIL029C_p)|FD-Score:3.35|P-value:4.03E-4||SGD DESC:Putative protein of unknown function; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has paralog YPR071W Gene:YIL096C(YIL096C_p)|FD-Score:5.12|P-value:1.51E-7||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis Gene:YJL160C(YJL160C_p)|FD-Score:3.6|P-value:1.58E-4||SGD DESC:Putative protein of unknown function; member of the PIR (proteins with internal repeats) family of cell wall proteins; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication Gene:YKR051W(YKR051W_p)|FD-Score:-3.21|P-value:6.72E-4||SGD DESC:Putative protein of unknown function Gene:YLR184W(YLR184W_d)|FD-Score:3.39|P-value:3.46E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C10.005.63E-246.10RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YMR033W3.934.31E-50.06ARP9Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
YIL126W3.875.43E-50.06STH1ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; essential helicase-related protein homologous to Snf2p
YIL022W3.816.97E-50.04TIM44Essential component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex)
YNL110C3.778.08E-50.14NOP15Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
YFR037C3.631.40E-40.04RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YBR237W3.591.67E-40.06PRP5RNA helicase in the DEAD-box family, necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
YOL144W3.532.08E-40.10NOP8Nucleolar protein required for 60S ribosomal subunit biogenesis
YNR016C3.433.03E-40.08ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YLR033W3.344.12E-40.16RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YNL221C3.197.16E-40.08POP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
YDL217C3.119.46E-40.03TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YPR187W3.080.001050.04RPO26RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
YLR208W3.040.001200.02SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YBR140C3.020.001270.02IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A20.903.48E-97TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YMR021C8.584.88E-18MAC1Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport
YDR127W7.464.39E-14ARO1Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
YOR036W5.961.28E-9PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YCR082W5.248.06E-8AHC2Component of the ADA histone acetyltransferase complex; Ach2p and Ach1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ach1p to the complex
YCR073W-A5.181.11E-7SOL2Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
YIL096C_p5.121.51E-7YIL096C_pPutative S-adenosylmethionine-dependent methyltransferase; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
YER086W4.865.79E-7ILV1Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
YDR495C4.731.14E-6VPS3Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
YLR044C4.661.56E-6PDC1Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism
YPL065W4.552.67E-6VPS28Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
YDL117W4.523.11E-6CYK3SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
YDL020C4.395.65E-6RPN4Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
YLR372W4.221.20E-5SUR4Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
YER107C4.181.48E-5GLE2RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1

GO enrichment analysis for SGTC_235
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.4730SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0289855TSC3-RPN4
0.4710SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.140625TSC3-RPN4
0.4433.19E-282SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.1TSC3-RPN4
0.4341.30E-269SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.55102TSC3-RPN4
0.4101.12E-237SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0952381TSC3-RPN4
0.3986.68E-223SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.08TSC3-RPN4
0.3933.25E-217SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.15873TSC3-RPN4
0.3935.79E-217SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0923077TSC3-RPN4
0.3816.41E-203SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.19697TSC3-RPN4
0.3771.88E-198SGTC_1662st014054 52.0 μMTimTec (Natural product derivative library)28387770.0581395TSC3-RPN4
0.3732.49E-194SGTC_328k915-0120 63.5 μMChemDiv (Drug-like library)66245430.0365854TSC3-RPN4
0.3721.89E-193SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.138462TSC3-RPN4
0.3682.55E-188SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0923077TSC3-RPN4
0.3661.15E-186SGTC_33179138495 8.8 μMChembridge (Drug-like library)170201390.0886076
0.3635.74E-184SGTC_20905301565 104.4 μMChembridge (Fragment library)20563440.0434783TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_520329-0193875 nM0.551023739292ChemDiv (Drug-like library)325.575642.80814TSC3-RPN4
SGTC_8360330-01041.35 μM0.42857146494820ChemDiv (Drug-like library)294.518543.6103
SGTC_9262268-140159.4 μM0.3833332931578ChemDiv (Drug-like library)390.388724.27926TSC3-RPN4
SGTC_5671080-054268.4 μM0.3684215950415ChemDiv (Drug-like library)296.277483.3115
SGTC_1813st0512468.6 μM0.3653855291959TimTec (Natural product derivative library)253.252743.59603TSC3-RPN4
SGTC_9691313-0238134 μM0.338715212874ChemDiv (Drug-like library)352.264753.37117mitochondrial processes
SGTC_5801123-002841 μM0.3275861677248ChemDiv (Drug-like library)359.334924.87805DNA damage response
SGTC_990109-0120622.8 μM0.314815759414ChemDiv (Drug-like library)321.126163.25713
SGTC_8610443-0273110 μM0.2982466149169ChemDiv (Drug-like library)268.267383.43914
SGTC_6371181-05197.26 μM0.2931036252091ChemDiv (Drug-like library)250.253860.70925fatty acid desaturase (OLE1)