9059630

4-chloro-2-morpholin-4-ylaniline

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2353
Screen concentration 146.7 μM
Source Chembridge (Fragment library)
PubChem CID 4779114
SMILES C1COCCN1C2=C(C=CC(=C2)Cl)N
Standardized SMILES Nc1ccc(Cl)cc1N2CCOCC2
Molecular weight 212.676
ALogP 1.6
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.44
% growth inhibition (Hom. pool) 4.91


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4779114
Download HIP data (tab-delimited text)  (excel)
Gene:APC2(YLR127C)|FD-Score:-5.76|P-value:4.21E-9|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:COF1(YLL050C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:EXO70(YJL085W)|FD-Score:-3.43|P-value:2.96E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.81||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NSE1(YLR007W)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.26||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RIB5(YBR256C)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL32(YBL092W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SMD2(YLR275W)|FD-Score:8.63|P-value:3.05E-18|Clearance:4.61||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNU13(YEL026W)|FD-Score:-3.13|P-value:8.66E-4|Clearance:0||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TSR1(YDL060W)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.03||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:APC2(YLR127C)|FD-Score:-5.76|P-value:4.21E-9|Clearance:0||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:COF1(YLL050C)|FD-Score:-3.19|P-value:7.21E-4|Clearance:0||SGD DESC:Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes Gene:EXO70(YJL085W)|FD-Score:-3.43|P-value:2.96E-4|Clearance:0||SGD DESC:Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress Gene:KAR1(YNL188W)|FD-Score:4.02|P-value:2.91E-5|Clearance:0.81||SGD DESC:Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p Gene:NSE1(YLR007W)|FD-Score:3.19|P-value:7.23E-4|Clearance:0.26||SGD DESC:Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair Gene:RIB5(YBR256C)|FD-Score:-3.72|P-value:1.01E-4|Clearance:0||SGD DESC:Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway Gene:RPL32(YBL092W)|FD-Score:-3.14|P-value:8.52E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog Gene:SMD2(YLR275W)|FD-Score:8.63|P-value:3.05E-18|Clearance:4.61||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:SNU13(YEL026W)|FD-Score:-3.13|P-value:8.66E-4|Clearance:0||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:TSR1(YDL060W)|FD-Score:3.21|P-value:6.59E-4|Clearance:0.03||SGD DESC:Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4779114
Download HOP data (tab-delimited text)  (excel)
Gene:AIM17(YHL021C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:EAF6(YJR082C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:FMP10(YER182W_p)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HIR1(YBL008W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HMF1(YER057C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:ICY1(YMR195W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IRC3(YDR332W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:MNN10(YDR245W)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN5(YJL186W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOD5(YOR274W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL16(YBL038W)|FD-Score:4.93|P-value:4.21E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NUR1(YDL089W)|FD-Score:-5.44|P-value:2.66E-8||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM1(YGR038W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PDX3(YBR035C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PUF4(YGL014W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAX1(YOR301W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPL27A(YHR010W)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL27B(YDR471W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHE1(YBL031W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE9(YDR393W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SSD1(YDR293C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH1(YBR283C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SWA2(YDR320C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TPP1(YMR156C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:6.04|P-value:7.54E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.74|P-value:4.66E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VAC14(YLR386W)|FD-Score:4.76|P-value:9.73E-7||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:YBL055C(YBL055C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR008C(YDR008C_d)|FD-Score:4.78|P-value:8.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL132W(YGL132W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YHI9(YHR029C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR125W(YHR125W_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKR047W(YKR047W_d)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR255C(YLR255C_d)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML090W(YML090W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YNL203C(YNL203C_d)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR200W(YOR200W_d)|FD-Score:4.5|P-value:3.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.27|P-value:6.96E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR Gene:AIM17(YHL021C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss Gene:AIM45(YPR004C)|FD-Score:3.45|P-value:2.79E-4||SGD DESC:Putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response Gene:EAF6(YJR082C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3 Gene:FMP10(YER182W_p)|FD-Score:4.66|P-value:1.60E-6||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:GCN2(YDR283C)|FD-Score:4.81|P-value:7.42E-7||SGD DESC:Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control Gene:GCN3(YKR026C)|FD-Score:3.13|P-value:8.66E-4||SGD DESC:Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression Gene:GCN4(YEL009C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels Gene:HIR1(YBL008W)|FD-Score:3.32|P-value:4.46E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores Gene:HMF1(YER057C)|FD-Score:3.24|P-value:5.96E-4||SGD DESC:Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro Gene:ICY1(YMR195W)|FD-Score:-3.2|P-value:6.79E-4||SGD DESC:Protein of unknown function, required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation Gene:IRC3(YDR332W)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Putative RNA helicase of the DEAH/D-box family; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion Gene:MNN10(YDR245W)|FD-Score:-3.2|P-value:6.96E-4||SGD DESC:Subunit of a Golgi mannosyltransferase complex also containing Anp1p, Mnn9p, Mnn11p, and Hoc1p that mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family Gene:MNN5(YJL186W)|FD-Score:3.16|P-value:7.86E-4||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MOD5(YOR274W)|FD-Score:-3.24|P-value:5.93E-4||SGD DESC:Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of the modified base isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; gene is nuclear and encodes two isozymic forms; converts to a prion form, and prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis Gene:MRPL16(YBL038W)|FD-Score:4.93|P-value:4.21E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:NUR1(YDL089W)|FD-Score:-5.44|P-value:2.66E-8||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:ORM1(YGR038W)|FD-Score:3.94|P-value:4.02E-5||SGD DESC:Evolutionarily conserved protein, similar to Orm2p, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control Gene:PDX3(YBR035C)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PUF4(YGL014W)|FD-Score:-3.58|P-value:1.74E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RAX1(YOR301W)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Protein involved in bud site selection during bipolar budding; localization requires Rax2p; has similarity to members of the insulin-related peptide superfamily Gene:RPL27A(YHR010W)|FD-Score:3.8|P-value:7.22E-5||SGD DESC:Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication Gene:RPL27B(YDR471W)|FD-Score:-3.18|P-value:7.32E-4||SGD DESC:Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:4.62|P-value:1.91E-6||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHE1(YBL031W)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function Gene:SHE9(YDR393W)|FD-Score:4.59|P-value:2.25E-6||SGD DESC:Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex Gene:SSD1(YDR293C)|FD-Score:4.72|P-value:1.17E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSH1(YBR283C)|FD-Score:3.64|P-value:1.37E-4||SGD DESC:Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential Gene:SWA2(YDR320C)|FD-Score:-3.64|P-value:1.38E-4||SGD DESC:Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles Gene:TPP1(YMR156C)|FD-Score:3.12|P-value:8.99E-4||SGD DESC:DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3′-phosphatase Gene:TRP1(YDR007W)|FD-Score:6.04|P-value:7.54E-10||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:4.4|P-value:5.50E-6||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP3(YKL211C)|FD-Score:5.74|P-value:4.66E-9||SGD DESC:Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p Gene:TRP4(YDR354W)|FD-Score:3.42|P-value:3.14E-4||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VAC14(YLR386W)|FD-Score:4.76|P-value:9.73E-7||SGD DESC:Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p Gene:YBL055C(YBL055C)|FD-Score:3.18|P-value:7.44E-4||SGD DESC:3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases Gene:YDL159W-A(YDL159W-A_p)|FD-Score:3.64|P-value:1.39E-4||SGD DESC:Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species Gene:YDR008C(YDR008C_d)|FD-Score:4.78|P-value:8.55E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YFL013W-A(YFL013W-A_d)|FD-Score:-3.15|P-value:8.10E-4||SGD DESC:Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching Gene:YGL132W(YGL132W_d)|FD-Score:3.6|P-value:1.57E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C Gene:YHI9(YHR029C)|FD-Score:3.14|P-value:8.52E-4||SGD DESC:Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production Gene:YHR125W(YHR125W_d)|FD-Score:3.22|P-value:6.44E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YIL054W(YIL054W_p)|FD-Score:3.74|P-value:9.28E-5||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels Gene:YKR047W(YKR047W_d)|FD-Score:4.47|P-value:3.97E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1 Gene:YLR255C(YLR255C_d)|FD-Score:3.77|P-value:8.22E-5||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML090W(YML090W_d)|FD-Score:3.94|P-value:4.04E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YNL203C(YNL203C_d)|FD-Score:5.57|P-value:1.29E-8||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR200W(YOR200W_d)|FD-Score:4.5|P-value:3.41E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c Gene:YPK1(YKL126W)|FD-Score:5.27|P-value:6.96E-8||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR123C(YPR123C_d)|FD-Score:3.36|P-value:3.85E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR275W8.633.05E-184.61SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YNL188W4.022.91E-50.81KAR1Essential protein involved in karyogamy during mating and in spindle pole body duplication during mitosis, localizes to the half-bridge of the spindle pole body, interacts with Spc72p during karyogamy, also interacts with Cdc31p
YDL060W3.216.59E-40.03TSR1Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
YLR007W3.197.23E-40.26NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YJL074C2.930.001700.06SMC3Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
YPR175W2.870.002060.06DPB2Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
YKR081C2.810.002510.08RPF2Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
YGR246C2.720.003240.03BRF1TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB
YDL212W2.690.003540.03SHR3Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
YHR062C2.670.003820.03RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGR060W2.640.004190.09ERG25C-4 methyl sterol oxidase, catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol
YOR250C2.550.005390.02CLP1Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
YPL093W2.530.005710.02NOG1Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
YBR135W2.510.006020.01CKS1Cyclin-dependent protein kinase regulatory subunit and adaptor; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters
YLR086W2.500.006280.02SMC4Subunit of the condensin complex; reorganizes chromosomes during cell division; forms a complex with Smc2p that has ATP-hydrolyzing and DNA-binding activity; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR007W6.047.54E-10TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YKL211C5.744.66E-9TRP3Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
YNL203C_d5.571.29E-8YNL203C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YKL126W5.276.96E-8YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YBL038W4.934.21E-7MRPL16Mitochondrial ribosomal protein of the large subunit
YDR283C4.817.42E-7GCN2Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
YDR008C_d4.788.55E-7YDR008C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YLR386W4.769.73E-7VAC14Protein involved in regulated synthesis of PtdIns(3,5)P(2), in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; interacts with Fig4p; activator of Fab1p
YEL009C4.731.12E-6GCN4Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
YDR293C4.721.17E-6SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YER182W_p4.661.60E-6FMP10_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YJR082C4.641.75E-6EAF6Subunit of the NuA4 acetyltransferase complex that acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
YDL168W4.621.91E-6SFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
YDR393W4.592.25E-6SHE9Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex
YOR200W_d4.503.41E-6YOR200W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c

GO enrichment analysis for SGTC_2353
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.3129.61E-133SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.105263
0.3046.16E-126SGTC_2758dehydroepiandrosterone 69.3 μMMiscellaneous58810.047619
0.3023.17E-124SGTC_2650prasterone 100.0 μMMicrosource (Natural product library)167592470.047619
0.3011.08E-123SGTC_23849036245 200.0 μMChembridge (Fragment library)64627660.173077
0.2981.89E-121SGTC_18825615643 20.0 μMMiscellaneous22530750.0985916TRP & mitochondrial translation
0.2974.09E-120SGTC_1572epiandrosterone 68.9 μMTimTec (Pure natural product library)4413020.03125
0.2933.43E-117SGTC_18835-benzylidenerhodanine 20.0 μMMiscellaneous12732100.0566038TRP & mitochondrial translation
0.2879.91E-112SGTC_1617st002906 78.0 μMTimTec (Natural product derivative library)5150859NA
0.2861.39E-111SGTC_14823346-2049 8.5 μMChemDiv (Drug-like library)38268040.0833333
0.2841.38E-109SGTC_2607solasodine 47.4 μMMicrosource (Natural product library)67106420.0493827
0.2842.57E-109SGTC_1801486-1293 102.8 μMChemDiv (Drug-like library)41405360.0555556
0.2796.94E-106SGTC_18815652484 16.0 μMMiscellaneous22551290.0694444TRP & mitochondrial translation
0.2791.13E-105SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0253165
0.2781.43E-104SGTC_14964469-0713 57.4 μMChemDiv (Drug-like library)7466910.0434783
0.2741.63E-101SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_20104012422159 μM0.473684952393Chembridge (Fragment library)212.676021.59713
SGTC_13561502-078185.8 μM0.413043567702ChemDiv (Drug-like library)284.761823.53804fatty acid desaturase (OLE1)
SGTC_22677931562200 μM0.391304934880Chembridge (Fragment library)267.754521.79313
SGTC_2377907335162.6 μM0.3913047337946Chembridge (Fragment library)281.78112.25613ergosterol biosynthesis
SGTC_2376907292281.32 μM0.37517087968Chembridge (Fragment library)281.78112.2513
SGTC_23479049783174.81 μM0.3617026467988Chembridge (Fragment library)267.754521.95413
SGTC_524r073-001027.8 μM0.3469392772106ChemDiv (Drug-like library)284.655861.80807
SGTC_14740330-01255.35 μM0.326923X1474ChemDiv (Drug-like library)405.531454.46630
SGTC_6751309-0189238 μM0.3220344246530ChemDiv (Drug-like library)435.2388023.43407
SGTC_213453248361.46 μM0.317073699272Chembridge (Fragment library)258.272561.23804