9061756

N-(7-methyl-2-oxo-1-propyl-3H-indol-3-yl)acetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2356
Screen concentration 189.0 μM
Source Chembridge (Fragment library)
PubChem CID 20104043
SMILES CCCN1C(=O)C(C2=C1C(=CC=C2)C)NC(=O)C
Standardized SMILES CCCN1C(=O)C(NC(=O)C)c2cccc(C)c12
Molecular weight 246.3049
ALogP 1.46
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.16
% growth inhibition (Hom. pool) 2.61


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 20104043
Download HIP data (tab-delimited text)  (excel)
Gene:EXO84(YBR102C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.04||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:FAS1(YKL182W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.16||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FOL2(YGR267C)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.06||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GLC7(YER133W)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MEX67(YPL169C)|FD-Score:-7.5|P-value:3.23E-14|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NUS1(YDL193W)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PAN1(YIR006C)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.08||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:RPT3(YDR394W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.02||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRN11(YML043C)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC31(YDL195W)|FD-Score:4.54|P-value:2.80E-6|Clearance:0.8||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC5(YDR166C)|FD-Score:3.26|P-value:5.60E-4|Clearance:0.03||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC9(YGR009C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.07||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SNU13(YEL026W)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:SPB4(YFL002C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.06||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients Gene:EXO84(YBR102C)|FD-Score:3.74|P-value:9.28E-5|Clearance:0.04||SGD DESC:Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate Gene:FAS1(YKL182W)|FD-Score:3.54|P-value:1.99E-4|Clearance:0.16||SGD DESC:Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities Gene:FOL2(YGR267C)|FD-Score:3.13|P-value:8.68E-4|Clearance:0.06||SGD DESC:GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway Gene:GLC7(YER133W)|FD-Score:-3.68|P-value:1.19E-4|Clearance:0||SGD DESC:Type 1 serine/threonine protein phosphatase catalytic subunit; involved in various processes including glycogen metabolism, sporulation, mitosis; accumulates at mating projections by interaction with Afr1p; interacts with many regulatory subunits; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; import into nucleus is inhibited during spindle assembly checkpoint arrest Gene:MEX67(YPL169C)|FD-Score:-7.5|P-value:3.23E-14|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:NUS1(YDL193W)|FD-Score:3.23|P-value:6.14E-4|Clearance:0.1||SGD DESC:Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY Gene:PAN1(YIR006C)|FD-Score:3.38|P-value:3.56E-4|Clearance:0.08||SGD DESC:Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease Gene:RPT3(YDR394W)|FD-Score:3.56|P-value:1.86E-4|Clearance:0.02||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B Gene:RPT5(YOR117W)|FD-Score:3.31|P-value:4.70E-4|Clearance:0.05||SGD DESC:One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1 Gene:RRN11(YML043C)|FD-Score:-4.17|P-value:1.53E-5|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:SEC31(YDL195W)|FD-Score:4.54|P-value:2.80E-6|Clearance:0.8||SGD DESC:Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance Gene:SEC5(YDR166C)|FD-Score:3.26|P-value:5.60E-4|Clearance:0.03||SGD DESC:Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip Gene:SEC9(YGR009C)|FD-Score:3.69|P-value:1.11E-4|Clearance:0.07||SGD DESC:t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog Gene:SNU13(YEL026W)|FD-Score:-3.3|P-value:4.86E-4|Clearance:0||SGD DESC:RNA binding protein, part of U3 snoRNP involved in rRNA processing, part of U4/U6-U5 tri-snRNP involved in mRNA splicing, similar to human 15.5K protein Gene:SPB4(YFL002C)|FD-Score:3.62|P-value:1.48E-4|Clearance:0.06||SGD DESC:Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 20104043
Download HOP data (tab-delimited text)  (excel)
Gene:ATG27(YJL178C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BEM4(YPL161C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUB2(YMR055C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CAR2(YLR438W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG1(YGL223C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUR1(YPR158W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CYK3(YDL117W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:ECM25(YJL201W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GEP4(YHR100C)|FD-Score:5.36|P-value:4.16E-8||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HBS1(YKR084C)|FD-Score:-5.63|P-value:8.79E-9||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HIS6(YIL020C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HOM6(YJR139C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HUL5(YGL141W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:LOT5(YKL183W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MMS1(YPR164W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MTQ1(YNL063W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NHX1(YDR456W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUR1(YDL089W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OMS1(YDR316W)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PDX3(YBR035C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:REG1(YDR028C)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:SAC6(YDR129C)|FD-Score:9.67|P-value:2.01E-22||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHS1(YDL225W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SKN7(YHR206W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SYF2(YGR129W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TKL1(YPR074C)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:VAM7(YGL212W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YGL138C(YGL138C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGL165C(YGL165C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL185C(YGL185C_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHC3(YJL059W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJL064W(YJL064W_d)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL030W(YKL030W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR012C(YKR012C_d)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Gene:YOL150C(YOL150C_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:ATG27(YJL178C)|FD-Score:3.86|P-value:5.65E-5||SGD DESC:Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site Gene:BEM4(YPL161C)|FD-Score:3.13|P-value:8.84E-4||SGD DESC:Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length Gene:BUB2(YMR055C)|FD-Score:-3.1|P-value:9.55E-4||SGD DESC:Mitotic exit network regulator, forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage Gene:CAR2(YLR438W)|FD-Score:3.1|P-value:9.58E-4||SGD DESC:L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress Gene:CCS1(YMR038C)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress Gene:COG1(YGL223C)|FD-Score:3.35|P-value:4.10E-4||SGD DESC:Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COG7(YGL005C)|FD-Score:4.7|P-value:1.31E-6||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:CUR1(YPR158W)|FD-Score:3.48|P-value:2.51E-4||SGD DESC:Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication Gene:CYK3(YDL117W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:ECM25(YJL201W)|FD-Score:3.6|P-value:1.61E-4||SGD DESC:Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p Gene:GEP4(YHR100C)|FD-Score:5.36|P-value:4.16E-8||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:HBS1(YKR084C)|FD-Score:-5.63|P-value:8.79E-9||SGD DESC:GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay Gene:HIS6(YIL020C)|FD-Score:-3.61|P-value:1.51E-4||SGD DESC:Phosphoribosyl-5-amino-1-phosphoribosyl-4-imidazolecarboxiamide isomerase, catalyzes the fourth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:HOM6(YJR139C)|FD-Score:3.79|P-value:7.57E-5||SGD DESC:Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions Gene:HUL5(YGL141W)|FD-Score:-3.21|P-value:6.74E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:LOT5(YKL183W)|FD-Score:3.16|P-value:7.94E-4||SGD DESC:Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress Gene:MMS1(YPR164W)|FD-Score:3.3|P-value:4.86E-4||SGD DESC:Subunit of an E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay Gene:MTQ1(YNL063W)|FD-Score:3.18|P-value:7.34E-4||SGD DESC:S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene Gene:NHX1(YDR456W)|FD-Score:3.2|P-value:6.90E-4||SGD DESC:Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion Gene:NUR1(YDL089W)|FD-Score:-3.2|P-value:6.84E-4||SGD DESC:Protein of unknown function; interacts with Csm1p, Lrs4p; required for rDNA repeat stability; null mutant causes increase in unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery, possible Cdc28p substrate Gene:OMS1(YDR316W)|FD-Score:3.88|P-value:5.28E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PDX3(YBR035C)|FD-Score:4.69|P-value:1.36E-6||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:REG1(YDR028C)|FD-Score:5.37|P-value:3.92E-8||SGD DESC:Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p Gene:SAC6(YDR129C)|FD-Score:9.67|P-value:2.01E-22||SGD DESC:Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:SHS1(YDL225W)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress Gene:SKN7(YHR206W)|FD-Score:4.54|P-value:2.85E-6||SGD DESC:Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication Gene:SYF2(YGR129W)|FD-Score:-4.22|P-value:1.21E-5||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TKL1(YPR074C)|FD-Score:5.5|P-value:1.89E-8||SGD DESC:Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication Gene:VAM7(YGL212W)|FD-Score:3.48|P-value:2.46E-4||SGD DESC:Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress Gene:VPS65(YLR322W_d)|FD-Score:4.19|P-value:1.41E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth Gene:YGL138C(YGL138C_p)|FD-Score:3.19|P-value:7.16E-4||SGD DESC:Putative protein of unknown function; has no significant sequence similarity to any known protein Gene:YGL165C(YGL165C_d)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W Gene:YGL185C(YGL185C_p)|FD-Score:-4.25|P-value:1.08E-5||SGD DESC:Putative protein with sequence similarity to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm Gene:YHC3(YJL059W)|FD-Score:3.42|P-value:3.19E-4||SGD DESC:Vacuolar membrane protein involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; homolog of human CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) Gene:YJL064W(YJL064W_d)|FD-Score:-4.23|P-value:1.16E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1 Gene:YKL030W(YKL030W_d)|FD-Score:3.7|P-value:1.08E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related <i>Saccharomyces</i> species; partially overlaps the verified gene MAE1 Gene:YKR012C(YKR012C_d)|FD-Score:-3.24|P-value:6.00E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2 Gene:YOL150C(YOL150C_d)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL195W4.542.80E-60.80SEC31Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
YBR102C3.749.28E-50.04EXO84Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
YGR009C3.691.11E-40.07SEC9t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
YFL002C3.621.48E-40.06SPB4Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
YDR394W3.561.86E-40.02RPT3One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
YKL182W3.541.99E-40.16FAS1Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
YIR006C3.383.56E-40.08PAN1Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on the cell cortex; promotes protein-protein interactions essential for endocytosis; binds to and activates the Arp2/3 complex in vitro; previously thought to be a subunit of poly(A) ribonuclease
YOR117W3.314.70E-40.05RPT5One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
YDR166C3.265.60E-40.03SEC5Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
YDL193W3.236.14E-40.10NUS1Putative prenyltransferase, required for cell viability; proposed to be involved in protein trafficking because tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY
YGR267C3.138.68E-40.06FOL2GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
YLR141W3.070.001050.03RRN5Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
YDL217C3.050.001160.00TIM22Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported
YGL093W3.040.001170.05SPC105Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
YLR198C_d2.990.001390.02YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDR129C9.672.01E-22SAC6Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
YPR074C5.501.89E-8TKL1Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
YDR028C5.373.92E-8REG1Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p
YHR100C5.364.16E-8GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YGL005C4.701.31E-6COG7Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
YBR035C4.691.36E-6PDX3Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
YHR206W4.542.85E-6SKN7Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
YLR322W_d4.191.41E-5VPS65_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth
YDL225W4.062.50E-5SHS1Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
YDR316W3.885.28E-5OMS1Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations
YJL178C3.865.65E-5ATG27Type I membrane protein involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; may be involved in membrane delivery to the phagophore assembly site
YJR139C3.797.57E-5HOM6Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
YKL030W_d3.701.08E-4YKL030W_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
YGL165C_d3.681.15E-4YGL165C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
YJL201W3.601.61E-4ECM25Non-essential protein of unknown function; promoter contains a consensus binding sequence for factor Abf1p

GO enrichment analysis for SGTC_2356
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1971.85E-52SGTC_23559061122 103.1 μMChembridge (Fragment library)53029570.169492
0.1625.62E-36SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.136364RPP1 & pyrimidine depletion
0.1529.92E-32SGTC_2681menadione 3.2 μMMiscellaneous40550.153846superoxide
0.1492.13E-30SGTC_1556plumbagin 1.5 μMTimTec (Pure natural product library)102050.157895superoxide
0.1431.86E-28SGTC_394menadione 8.3 μMMiscellaneous40550.153846superoxide
0.1405.39E-27SGTC_23579067248 200.0 μMChembridge (Fragment library)91502350.111111
0.1346.26E-25SGTC_8940960-0238 55.9 μMChemDiv (Drug-like library)67419070.113924
0.1271.07E-22SGTC_23549060492 162.0 μMChembridge (Fragment library)64718950.126984
0.1263.13E-22SGTC_13001094-0077 113.0 μMChemDiv (Drug-like library)55326360.12987
0.1248.70E-22SGTC_22597964009 200.0 μMChembridge (Fragment library)3131340.189655superoxide
0.1232.41E-21SGTC_1772st048439 82.2 μMTimTec (Natural product derivative library)29270680.15625
0.1181.05E-19SGTC_15526-methylflavone 10.6 μMTimTec (Pure natural product library)6890130.126984superoxide
0.1163.29E-19SGTC_22667929956 200.0 μMChembridge (Fragment library)29671530.37037
0.1111.32E-17SGTC_7991310-0557 151.0 μMChemDiv (Drug-like library)53544920.106061superoxide
0.1111.51E-17SGTC_1994st072947 40.9 μMTimTec (Natural product derivative library)52865650.102564

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2945906768161.76 μM0.44067816456930Chembridge (Drug-like library)344.792182.40113
SGTC_22667929956200 μM0.370372967153Chembridge (Fragment library)238.670240.76912
SGTC_22137173844200 μM0.322034892728Chembridge (Fragment library)259.300382.62204
SGTC_2200660060421.91 μM0.3076922904777Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_3281914061349.47 μM0.28787916013766Chembridge (Drug-like library)361.436923.12703RPP1 & pyrimidine depletion
SGTC_2828799886171.43 μM0.2698412988188Chembridge (Drug-like library)325.358463.0404
SGTC_22016605497169.86 μM0.254545670741Chembridge (Fragment library)232.32142.18102ergosterol biosynthesis
SGTC_21735763948167 μM0.2456142867177Chembridge (Fragment library)269.29521.81603tubulin folding & SWR complex
SGTC_2246729350791.56 μM0.245614887656Chembridge (Fragment library)244.245980.99413
SGTC_23459052315157.45 μM0.2456146469265Chembridge (Fragment library)193.24561.48922