9040895

5'-chlorospiro[1,3-dioxane-2,3'-1H-indole]-2'-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2360
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 4914414
SMILES C1COC2(C3=C(C=CC(=C3)Cl)NC2=O)OC1
Standardized SMILES Clc1ccc2NC(=O)C3(OCCCO3)c2c1
Molecular weight 239.655
ALogP 1.25
H-bond donor count 1
H-bond acceptor count 3
Response signature RPP1 & pyrimidine depletion

Pool Growth Kinetics
% growth inhibition (Het. pool) 7.24
% growth inhibition (Hom. pool) 6.04


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4914414
Download HIP data (tab-delimited text)  (excel)
Gene:ABD1(YBR236C)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.54||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:APC2(YLR127C)|FD-Score:6.73|P-value:8.71E-12|Clearance:0.55||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.33||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GPN2(YOR262W)|FD-Score:-4.14|P-value:1.73E-5|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KEI1(YDR367W)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.03||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MEC1(YBR136W)|FD-Score:3.86|P-value:5.72E-5|Clearance:0.04||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NNF1(YJR112W)|FD-Score:7.2|P-value:2.97E-13|Clearance:0.48||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NSL1(YPL233W)|FD-Score:3.82|P-value:6.69E-5|Clearance:0.14||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:4.78|P-value:8.87E-7|Clearance:0.19||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRI2(YKL045W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.05||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.06||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPP1(YHR062C)|FD-Score:6.18|P-value:3.27E-10|Clearance:1.4||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SKI6(YGR195W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SMC2(YFR031C)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:SPT14(YPL175W)|FD-Score:3.17|P-value:7.65E-4|Clearance:0.02||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:STN1(YDR082W)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.16||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TAF8(YML114C)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.06||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:UTP5(YDR398W)|FD-Score:9.31|P-value:6.26E-21|Clearance:2.11||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VHT1(YGR065C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin Gene:ABD1(YBR236C)|FD-Score:4.59|P-value:2.23E-6|Clearance:0.54||SGD DESC:Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA Gene:APC2(YLR127C)|FD-Score:6.73|P-value:8.71E-12|Clearance:0.55||SGD DESC:Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p Gene:ERG12(YMR208W)|FD-Score:3.09|P-value:9.95E-4|Clearance:0.04||SGD DESC:Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate Gene:ESP1(YGR098C)|FD-Score:3.62|P-value:1.46E-4|Clearance:0.33||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:GPN2(YOR262W)|FD-Score:-4.14|P-value:1.73E-5|Clearance:0||SGD DESC:Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion Gene:KEI1(YDR367W)|FD-Score:4.05|P-value:2.58E-5|Clearance:0.03||SGD DESC:Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene Gene:MEC1(YBR136W)|FD-Score:3.86|P-value:5.72E-5|Clearance:0.04||SGD DESC:Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres Gene:NNF1(YJR112W)|FD-Score:7.2|P-value:2.97E-13|Clearance:0.48||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NSL1(YPL233W)|FD-Score:3.82|P-value:6.69E-5|Clearance:0.14||SGD DESC:Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation Gene:NUT2(YPR168W)|FD-Score:4.78|P-value:8.87E-7|Clearance:0.19||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress Gene:POP4(YBR257W)|FD-Score:-3.43|P-value:2.99E-4|Clearance:0||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P Gene:PRI2(YKL045W)|FD-Score:3.15|P-value:8.27E-4|Clearance:0.05||SGD DESC:Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair Gene:PRP16(YKR086W)|FD-Score:3.68|P-value:1.17E-4|Clearance:0.06||SGD DESC:DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step Gene:RPP1(YHR062C)|FD-Score:6.18|P-value:3.27E-10|Clearance:1.4||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SKI6(YGR195W)|FD-Score:3.24|P-value:6.02E-4|Clearance:0.07||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4) Gene:SMC2(YFR031C)|FD-Score:-3.38|P-value:3.68E-4|Clearance:0||SGD DESC:Subunit of the condensin complex; essential SMC chromosomal ATPase family member that forms a complex with Smc4p to form the active ATPase; Smc2p/Smc4p complex binds DNA; required for clustering of tRNA genes at the nucleolus Gene:SPT14(YPL175W)|FD-Score:3.17|P-value:7.65E-4|Clearance:0.02||SGD DESC:UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins Gene:STN1(YDR082W)|FD-Score:4.02|P-value:2.92E-5|Clearance:0.16||SGD DESC:Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping Gene:TAF8(YML114C)|FD-Score:3.3|P-value:4.92E-4|Clearance:0.06||SGD DESC:TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation Gene:UTP5(YDR398W)|FD-Score:9.31|P-value:6.26E-21|Clearance:2.11||SGD DESC:Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit Gene:VHT1(YGR065C)|FD-Score:-3.4|P-value:3.41E-4|Clearance:0||SGD DESC:High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4914414
Download HOP data (tab-delimited text)  (excel)
Gene:AFT1(YGL071W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC1(YOR023C)|FD-Score:-4.51|P-value:3.31E-6||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD2(YMR170C)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALP1(YNL270C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:APE4(YHR113W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARG80(YMR042W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARP1(YHR129C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BPT1(YLL015W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:CLU1(YMR012W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:DID4(YKL002W)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:FMP10(YER182W_p)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:-5.34|P-value:4.60E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV12(YOR183W_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:5.45|P-value:2.57E-8||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL11(YOL051W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCS1(YDL226C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HIS5(YIL116W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:INO2(YDR123C)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:ISU2(YOR226C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LCB4(YOR171C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LYS2(YBR115C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MEH1(YKR007W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRH1(YDR033W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSA2(YKR077W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTD1(YKR080W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NUP42(YDR192C)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:PST1(YDR055W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTK2(YJR059W)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS1(YPL212C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RAD51(YER095W)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RBG1(YAL036C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RLM1(YPL089C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROX1(YPR065W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RVS161(YCR009C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCM4(YGR049W)|FD-Score:-4.65|P-value:1.62E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SPT10(YJL127C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:STI1(YOR027W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWC7(YLR385C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:SYF2(YGR129W)|FD-Score:7.2|P-value:3.00E-13||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA6(YPR157W_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS27(YNR006W)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP5(YIR018W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL012C(YBL012C_d)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR099C(YBR099C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YCR016W(YCR016W_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR087C-A(YCR087C-A_p)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-5.84|P-value:2.68E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:6.04|P-value:7.78E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL088W(YGL088W_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL206C(YJL206C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YOL057W(YOL057W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL114C(YOL114C_p)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:6.28|P-value:1.70E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AHC1(YOR023C)|FD-Score:-4.51|P-value:3.31E-6||SGD DESC:Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex Gene:ALD2(YMR170C)|FD-Score:-3.91|P-value:4.71E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALP1(YNL270C)|FD-Score:3.14|P-value:8.43E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:APE4(YHR113W)|FD-Score:3.09|P-value:9.99E-4||SGD DESC:Cytoplasmic aspartyl aminopeptidase that may also function in the vacuole; CVT pathway cargo protein; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms a 12-subunit homo-oligomer; M18 metalloprotease family Gene:ARG80(YMR042W)|FD-Score:3.2|P-value:6.92E-4||SGD DESC:Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p Gene:ARP1(YHR129C)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin Gene:BPT1(YLL015W)|FD-Score:-3.68|P-value:1.16E-4||SGD DESC:ABC type transmembrane transporter of MRP/CFTR family, found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p Gene:CLU1(YMR012W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:eIF3 component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation, can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant Gene:DID4(YKL002W)|FD-Score:4.72|P-value:1.16E-6||SGD DESC:Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis Gene:FMP10(YER182W_p)|FD-Score:5.03|P-value:2.50E-7||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FUR4(YBR021W)|FD-Score:-5.34|P-value:4.60E-8||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV12(YOR183W_p)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Protein of unknown function, required for survival upon exposure to K1 killer toxin Gene:FYV5(YCL058C)|FD-Score:5.45|P-value:2.57E-8||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GAL11(YOL051W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator Gene:GCS1(YDL226C)|FD-Score:4.01|P-value:3.06E-5||SGD DESC:ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p Gene:HIS5(YIL116W)|FD-Score:-3.42|P-value:3.13E-4||SGD DESC:Histidinol-phosphate aminotransferase, catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts Gene:INO2(YDR123C)|FD-Score:-3.95|P-value:3.98E-5||SGD DESC:Component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion Gene:ISU2(YOR226C)|FD-Score:3.39|P-value:3.51E-4||SGD DESC:Protein required for synthesis of iron-sulfur proteins; localized to the mitochondrial matrix; performs a scaffolding function in mitochondria during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; isu1 isu2 double mutant is inviable; protein abundance increases in response to DNA replication stress; evolutionarily conserved Gene:LCB4(YOR171C)|FD-Score:3.24|P-value:6.00E-4||SGD DESC:Sphingoid long-chain base kinase, responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes Gene:LYS2(YBR115C)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Alpha aminoadipate reductase, catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p Gene:MEH1(YKR007W)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification Gene:MRH1(YDR033W)|FD-Score:3.68|P-value:1.19E-4||SGD DESC:Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; MRH1 has a paralog, YRO2, that arose from the whole genome duplication Gene:MSA2(YKR077W)|FD-Score:4.26|P-value:1.00E-5||SGD DESC:Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation Gene:MTD1(YKR080W)|FD-Score:-3.18|P-value:7.45E-4||SGD DESC:NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase, plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline Gene:NUP42(YDR192C)|FD-Score:-4.7|P-value:1.28E-6||SGD DESC:FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p Gene:PST1(YDR055W)|FD-Score:3.19|P-value:7.02E-4||SGD DESC:Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1 Gene:PTK2(YJR059W)|FD-Score:-3.85|P-value:5.89E-5||SGD DESC:Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake Gene:PUS1(YPL212C)|FD-Score:3.35|P-value:3.98E-4||SGD DESC:tRNA:pseudouridine synthase, introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; nuclear protein that appears to be involved in tRNA export; also acts on U2 snRNA Gene:RAD51(YER095W)|FD-Score:6.23|P-value:2.28E-10||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RBG1(YAL036C)|FD-Score:3.89|P-value:5.10E-5||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RLM1(YPL089C)|FD-Score:3.45|P-value:2.84E-4||SGD DESC:MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication Gene:ROX1(YPR065W)|FD-Score:-3.32|P-value:4.57E-4||SGD DESC:Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance Gene:RVS161(YCR009C)|FD-Score:3.22|P-value:6.46E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SCM4(YGR049W)|FD-Score:-4.65|P-value:1.62E-6||SGD DESC:Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation Gene:SPT10(YJL127C)|FD-Score:3.46|P-value:2.69E-4||SGD DESC:Putative histone acetylase with a role in transcriptional silencing, sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near the TATA box Gene:STI1(YOR027W)|FD-Score:-3.35|P-value:3.99E-4||SGD DESC:Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop Gene:SWC7(YLR385C)|FD-Score:-3.75|P-value:8.84E-5||SGD DESC:Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin Gene:SYF2(YGR129W)|FD-Score:7.2|P-value:3.00E-13||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TDA6(YPR157W_p)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele Gene:TRP1(YDR007W)|FD-Score:3.11|P-value:9.34E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TRP2(YER090W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:TRP4(YDR354W)|FD-Score:4.98|P-value:3.11E-7||SGD DESC:Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis Gene:VPS27(YNR006W)|FD-Score:6.59|P-value:2.26E-11||SGD DESC:Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p) Gene:YAP5(YIR018W)|FD-Score:-3.79|P-value:7.52E-5||SGD DESC:Basic leucine zipper (bZIP) iron-sensing transcription factor; YAP5 has a paralog, YAP7, that arose from the whole genome duplication Gene:YBL012C(YBL012C_d)|FD-Score:3.76|P-value:8.34E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W-A(YBR056W-A_p)|FD-Score:3.64|P-value:1.36E-4||SGD DESC:Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the dubious ORF YBR056C-B Gene:YBR099C(YBR099C_d)|FD-Score:4.06|P-value:2.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4 Gene:YCR016W(YCR016W_p)|FD-Score:-3.41|P-value:3.27E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR087C-A(YCR087C-A_p)|FD-Score:5.01|P-value:2.75E-7||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YDL187C(YDL187C_d)|FD-Score:-5.84|P-value:2.68E-9||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YDR008C(YDR008C_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YDR182W-A(YDR182W-A_p)|FD-Score:3.74|P-value:9.21E-5||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YDR455C(YDR455C_d)|FD-Score:6.04|P-value:7.78E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W Gene:YGL088W(YGL088W_d)|FD-Score:-3.31|P-value:4.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps snR10, a snoRNA required for preRNA processing Gene:YIR018C-A(YIR018C-A_p)|FD-Score:3.26|P-value:5.48E-4||SGD DESC:Putative protein of unknown function; identified by expression profiling and mass spectrometry Gene:YJL206C(YJL206C_p)|FD-Score:4.26|P-value:1.01E-5||SGD DESC:Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment Gene:YKL162C-A(YKL162C-A_d)|FD-Score:3.58|P-value:1.74E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR374C(YLR374C_d)|FD-Score:3.63|P-value:1.39E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W Gene:YOL057W(YOL057W)|FD-Score:3.19|P-value:7.04E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL114C(YOL114C_p)|FD-Score:3.15|P-value:8.04E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YOR385W(YOR385W_p)|FD-Score:5.57|P-value:1.28E-8||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:6.28|P-value:1.70E-10||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDR398W9.316.26E-212.11UTP5Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
YJR112W7.202.97E-130.48NNF1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YLR127C6.738.71E-120.55APC2Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
YHR062C6.183.27E-101.40RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YPR168W4.788.87E-70.19NUT2Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
YBR236C4.592.23E-60.54ABD1Methyltransferase, catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA
YDR367W4.052.58E-50.03KEI1Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
YDR082W4.022.92E-50.16STN1Telomere end-binding and capping protein, plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping
YBR136W3.865.72E-50.04MEC1Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
YPL233W3.826.69E-50.14NSL1Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation
YKR086W3.681.17E-40.06PRP16DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step
YGR098C3.621.46E-40.33ESP1Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
YML114C3.304.92E-40.06TAF8TFIID subunit (65 kDa), involved in RNA polymerase II transcription initiation
YGR195W3.246.02E-40.07SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YPL175W3.177.65E-40.02SPT14UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YGR129W7.203.00E-13SYF2Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest
YNR006W6.592.26E-11VPS27Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
YKL126W6.281.70E-10YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YER095W6.232.28E-10RAD51Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
YDR455C_d6.047.78E-10YDR455C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
YOR385W_p5.571.28E-8YOR385W_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
YCL058C5.452.57E-8FYV5Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis
YER182W_p5.032.50E-7FMP10_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YCR087C-A_p5.012.75E-7YCR087C-A_pPutative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
YDR354W4.983.11E-7TRP4Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
YKL002W4.721.16E-6DID4Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
YKR077W4.261.00E-5MSA2Putative transcriptional activator, that interacts with G1-specific transcription factor, MBF and G1-specific promoters; ortholog of Msa2p, an MBF and SBF activator that regulates G1-specific transcription and cell cycle initiation
YJL206C_p4.261.01E-5YJL206C_pPutative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment
YBR099C_d4.062.44E-5YBR099C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
YDL226C4.013.06E-5GCS1ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p

GO enrichment analysis for SGTC_2360
biological processes

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1452.60E-29SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.106667
0.1426.32E-28SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.115385plasma membrane duress
0.1323.40E-24SGTC_21986589944 200.0 μMChembridge (Fragment library)52902680.163934
0.1314.26E-24SGTC_2733amorolfine 100.0 μMMiscellaneous542600.0746269plasma membrane duress
0.1293.77E-23SGTC_2618avocadyne 43.4 μMMicrosource (Natural product library)3015189NA
0.1263.23E-22SGTC_2725haloperidol 53.2 μMMiscellaneous35590.149254plasma membrane duress
0.1241.02E-21SGTC_2741miltefosine 3.0 μMMiscellaneous35990.0166667
0.1163.77E-19SGTC_2524avocatin b 10.6 μMMicrosource (Natural product library)6710748NA
0.1164.19E-19SGTC_2642cholecalciferol 100.0 μMMicrosource (Natural product library)98214650.0487805
0.1151.07E-18SGTC_24885268135 45.7 μMMiscellaneous12703560.12069
0.1119.84E-18SGTC_1999st074831 12.5 μMTimTec (Natural product derivative library)171125230.184615plasma membrane duress
0.1102.28E-17SGTC_240417-1663 219.0 μMChemDiv (Drug-like library)43293900.0769231
0.1103.18E-17SGTC_1697st025554 27.7 μMTimTec (Natural product derivative library)12695190.172414
0.1094.40E-17SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.078125
0.1096.00E-17SGTC_18815652484 16.0 μMMiscellaneous22551290.0789474TRP & mitochondrial translation

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23579067248200 μM0.4545459150235Chembridge (Fragment library)225.628421.18413
SGTC_22226885208172.14 μM0.422222609514Chembridge (Fragment library)233.26311.43313
SGTC_1090efavirenz20.7 μM0.37564139Miscellaneous315.674974.38115
SGTC_2270792457590.91 μM0.3333331262135Chembridge (Fragment library)241.3050232.21414RPP1 & pyrimidine depletion
SGTC_465cgp-3715741 μM0.2909092688ICCB bioactive library324.224944.12612
SGTC_2405ticlatone3.52 μM0.260876258Miscellaneous185.630762.03912
SGTC_23539059630146.67 μM0.2448984779114Chembridge (Fragment library)212.676021.59713
SGTC_3239913375749.47 μM0.24193517036923Chembridge (Drug-like library)301.767443.42423
SGTC_20595238311157 μM0.236364703021Chembridge (Fragment library)251.66571.75714
SGTC_1723st0343072.55 μM0.235294867466TimTec (Natural product derivative library)297.949563.81402mitochondrial processes