9055074

1-(3-chloro-2-methylphenyl)-3-(pyridin-4-ylmethyl)urea

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2364
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 6470630
SMILES CC1=C(C=CC=C1Cl)NC(=O)NCC2=CC=NC=C2
Standardized SMILES Cc1c(Cl)cccc1NC(=O)NCc2ccncc2
Molecular weight 275.7335
ALogP 2.53
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 0.36
% growth inhibition (Hom. pool) 1.12


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6470630
Download HIP data (tab-delimited text)  (excel)
Gene:ARC40(YBR234C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.24||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASA1(YPR085C)|FD-Score:3.28|P-value:5.28E-4|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB1(YDR531W)|FD-Score:-4.03|P-value:2.79E-5|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CDC9(YDL164C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:FMN1(YDR236C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:MEX67(YPL169C)|FD-Score:-4.5|P-value:3.34E-6|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MRPS18(YNL306W)|FD-Score:-3.58|P-value:1.75E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:RRN11(YML043C)|FD-Score:-5.21|P-value:9.28E-8|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:4.75|P-value:1.02E-6|Clearance:1.15||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:TIF35(YDR429C)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:4.42|P-value:4.83E-6|Clearance:1.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR073C(YGR073C_d)|FD-Score:4.67|P-value:1.54E-6|Clearance:1.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein Gene:ARC40(YBR234C)|FD-Score:3.27|P-value:5.47E-4|Clearance:0.24||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ASA1(YPR085C)|FD-Score:3.28|P-value:5.28E-4|Clearance:0.01||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1 Gene:CAB1(YDR531W)|FD-Score:-4.03|P-value:2.79E-5|Clearance:0||SGD DESC:Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element Gene:CDC9(YDL164C)|FD-Score:-3.48|P-value:2.54E-4|Clearance:0||SGD DESC:DNA ligase found in the nucleus and mitochondria, an essential enzyme that joins Okazaki fragments during DNA replication; also acts in nucleotide excision repair, base excision repair, and recombination Gene:FMN1(YDR236C)|FD-Score:-3.49|P-value:2.41E-4|Clearance:0||SGD DESC:Riboflavin kinase, produces riboflavin monophosphate (FMN); FMN is a necessary cofactor for many enzymes; predominantly localizes to the microsomal fraction and also found in the mitochondrial inner membrane Gene:GLE1(YDL207W)|FD-Score:-3.21|P-value:6.63E-4|Clearance:0||SGD DESC:Cytoplasmic nucleoporin required for polyadenylated RNA export; not essential for protein import; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export Gene:MEX67(YPL169C)|FD-Score:-4.5|P-value:3.34E-6|Clearance:0||SGD DESC:Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP Gene:MRPS18(YNL306W)|FD-Score:-3.58|P-value:1.75E-4|Clearance:0||SGD DESC:Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins Gene:RRN11(YML043C)|FD-Score:-5.21|P-value:9.28E-8|Clearance:0||SGD DESC:Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p Gene:RRP4(YHR069C)|FD-Score:4.75|P-value:1.02E-6|Clearance:1.15||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2) Gene:TIF35(YDR429C)|FD-Score:-3.1|P-value:9.67E-4|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:YDL196W(YDL196W_d)|FD-Score:4.42|P-value:4.83E-6|Clearance:1.15||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31 Gene:YGR073C(YGR073C_d)|FD-Score:4.67|P-value:1.54E-6|Clearance:1.15||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6470630
Download HOP data (tab-delimited text)  (excel)
Gene:AIM21(YIR003W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:AMD1(YML035C)|FD-Score:-5.43|P-value:2.79E-8||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:APS3(YJL024C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:AQR1(YNL065W)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ARC1(YGL105W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ASM4(YDL088C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ASN1(YPR145W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BIO2(YGR286C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BMH2(YDR099W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRO1(YPL084W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:COX19(YLL018C-A)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX8(YLR395C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUL3(YGR003W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DAL4(YIR028W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DGR2(YKL121W_p)|FD-Score:-4.34|P-value:7.17E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA4(YHR011W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DTR1(YBR180W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:ERD1(YDR414C)|FD-Score:-4.78|P-value:8.85E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERP5(YHR110W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FMP41(YNL168C_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GEA1(YJR031C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIC1(YHR061C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GRX4(YER174C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HAP3(YBL021C)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HOS2(YGL194C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPR1(YDR138W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HUG1(YML058W-A)|FD-Score:-3.86|P-value:5.73E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:HXT8(YJL214W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IMD3(YLR432W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IMO32(YGR031W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRC18(YJL037W)|FD-Score:7.7|P-value:6.89E-15||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JIP4(YDR475C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KKQ8(YKL168C)|FD-Score:6.41|P-value:7.40E-11||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LSM12(YHR121W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAE1(YKL029C)|FD-Score:7.15|P-value:4.45E-13||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDL1(YLR188W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MIG2(YGL209W)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL20(YKR085C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NDL1(YLR254C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:OMS1(YDR316W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDX3(YBR035C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKH3(YDR466W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT1(YDL095W)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PRY2(YKR013W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC8(YPR007C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:REH1(YLR387C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF2(YLR453C)|FD-Score:4.79|P-value:8.49E-7||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM4(YHL024W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMA1(YKL132C)|FD-Score:5.96|P-value:1.26E-9||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL23B(YER117W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:4.85|P-value:6.08E-7||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SFT2(YBL102W)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SLX8(YER116C)|FD-Score:3.74|P-value:9.11E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNZ1(YMR096W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins Gene:SOD1(YJR104C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STE13(YOR219C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SWF1(YDR126W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYF2(YGR129W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAX4(YJL083W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM82(YDR165W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:-4.96|P-value:3.51E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC7(YMR022W)|FD-Score:8.36|P-value:3.18E-17||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBX2(YML013W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA16(YHR026W)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS55(YJR044C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL066W(YAL066W_d)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAP1(YML007W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YER184C(YER184C_p)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YLR278C(YLR278C_p)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YME2(YMR302C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML018C(YML018C_p)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML116W-A(YML116W-A_d)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative protein of unknown function Gene:YNL155W(YNL155W_p)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL057W(YOL057W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL114C(YOL114C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPR195C(YPR195C_d)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:6.21|P-value:2.70E-10||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p Gene:AIM21(YIR003W)|FD-Score:3.3|P-value:4.81E-4||SGD DESC:Protein of unknown function involved in mitochondrial migration along actin filament; may interact with ribosomes; GFP-fusion protein colocalizes with Sac1p to the actin cytoskeleton Gene:AMD1(YML035C)|FD-Score:-5.43|P-value:2.79E-8||SGD DESC:AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools Gene:APS3(YJL024C)|FD-Score:3.77|P-value:8.29E-5||SGD DESC:Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress Gene:AQR1(YNL065W)|FD-Score:-3.84|P-value:6.18E-5||SGD DESC:Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress Gene:ARC1(YGL105W)|FD-Score:3.27|P-value:5.33E-4||SGD DESC:Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases (Mes1p and Gus1p), delivering tRNA to them, stimulating catalysis, and ensuring their localization to the cytoplasm; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress Gene:ASI1(YMR119W)|FD-Score:3.33|P-value:4.41E-4||SGD DESC:Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication Gene:ASM4(YDL088C)|FD-Score:3.11|P-value:9.32E-4||SGD DESC:FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication Gene:ASN1(YPR145W)|FD-Score:-3.25|P-value:5.87E-4||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BIO2(YGR286C)|FD-Score:-3.56|P-value:1.84E-4||SGD DESC:Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant Gene:BMH2(YDR099W)|FD-Score:3.63|P-value:1.43E-4||SGD DESC:14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress Gene:BRO1(YPL084W)|FD-Score:3.41|P-value:3.22E-4||SGD DESC:Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes Gene:COX19(YLL018C-A)|FD-Score:4.1|P-value:2.06E-5||SGD DESC:Protein required for cytochrome c oxidase assembly, located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs Gene:COX8(YLR395C)|FD-Score:5.3|P-value:5.65E-8||SGD DESC:Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain Gene:CUL3(YGR003W)|FD-Score:-3.59|P-value:1.68E-4||SGD DESC:Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 Gene:DAL4(YIR028W)|FD-Score:3.25|P-value:5.76E-4||SGD DESC:Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DGR2(YKL121W_p)|FD-Score:-4.34|P-value:7.17E-6||SGD DESC:Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds Gene:DIA4(YHR011W)|FD-Score:-3.38|P-value:3.59E-4||SGD DESC:Probable mitochondrial seryl-tRNA synthetase, mutant displays increased invasive and pseudohyphal growth Gene:DTR1(YBR180W)|FD-Score:3.14|P-value:8.41E-4||SGD DESC:Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; expressed during sporulation Gene:ERD1(YDR414C)|FD-Score:-4.78|P-value:8.85E-7||SGD DESC:Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p) Gene:ERP5(YHR110W)|FD-Score:-3.45|P-value:2.84E-4||SGD DESC:Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport Gene:FMP41(YNL168C_p)|FD-Score:4.23|P-value:1.18E-5||SGD DESC:Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FRA2(YGL220W)|FD-Score:-3.38|P-value:3.58E-4||SGD DESC:Protein involved in negative regulation of transcription of iron regulon; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; null mutant fails to repress iron regulon and is sensitive to nickel Gene:GEA1(YJR031C)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GIC1(YHR061C)|FD-Score:-3.4|P-value:3.40E-4||SGD DESC:Protein involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; relocalizes from bud neck to nucleus upon DNA replication stress Gene:GRX4(YER174C)|FD-Score:3.25|P-value:5.77E-4||SGD DESC:Hydroperoxide and superoxide-radical responsive glutathione-dependent oxidoreductase; monothiol glutaredoxin subfamily member along with Grx3p and Grx5p; protects cells from oxidative damage; mutant has increased aneuploidy tolerance; transcription is regulated by Yap5 Gene:HAP3(YBL021C)|FD-Score:4.56|P-value:2.53E-6||SGD DESC:Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding Gene:HOS2(YGL194C)|FD-Score:-3.65|P-value:1.32E-4||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:HPR1(YDR138W)|FD-Score:-3.41|P-value:3.25E-4||SGD DESC:Subunit of THO/TREX complexes that couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p Gene:HUG1(YML058W-A)|FD-Score:-3.86|P-value:5.73E-5||SGD DESC:Protein involved in the Mec1p-mediated checkpoint pathway; transcription is induced by DNA damage; protein abundance increases in response to DNA replication stress Gene:HXT8(YJL214W)|FD-Score:-3.16|P-value:7.83E-4||SGD DESC:Protein of unknown function with similarity to hexose transporter family members, expression is induced by low levels of glucose and repressed by high levels of glucose Gene:IMD3(YLR432W)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Inosine monophosphate dehydrogenase, catalyzes the first step of GMP biosynthesis, member of a four-gene family in S. cerevisiae, constitutively expressed Gene:IMO32(YGR031W)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1 Gene:IRC18(YJL037W)|FD-Score:7.7|P-value:6.89E-15||SGD DESC:Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci Gene:JIP4(YDR475C)|FD-Score:-3.39|P-value:3.46E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:KKQ8(YKL168C)|FD-Score:6.41|P-value:7.40E-11||SGD DESC:Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication Gene:LSB5(YCL034W)|FD-Score:3.9|P-value:4.85E-5||SGD DESC:Protein of unknown function; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; may mediate disassembly of the Pan1 complex from the endocytic coat Gene:LSM12(YHR121W)|FD-Score:3.7|P-value:1.07E-4||SGD DESC:Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress Gene:MAE1(YKL029C)|FD-Score:7.15|P-value:4.45E-13||SGD DESC:Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids Gene:MDL1(YLR188W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress Gene:MIG2(YGL209W)|FD-Score:4.53|P-value:2.96E-6||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MRPL20(YKR085C)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSW1(YDR268W)|FD-Score:-3.19|P-value:7.07E-4||SGD DESC:Mitochondrial tryptophanyl-tRNA synthetase Gene:NDL1(YLR254C)|FD-Score:3.13|P-value:8.63E-4||SGD DESC:Homolog of nuclear distribution factor NudE, NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends Gene:OMS1(YDR316W)|FD-Score:3.84|P-value:6.22E-5||SGD DESC:Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif; multicopy suppressor of respiratory defects caused by OXA1 mutations Gene:PBP4(YDL053C)|FD-Score:3.25|P-value:5.85E-4||SGD DESC:Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress Gene:PDX3(YBR035C)|FD-Score:3.99|P-value:3.36E-5||SGD DESC:Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism Gene:PKH3(YDR466W)|FD-Score:4.18|P-value:1.45E-5||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PMT1(YDL095W)|FD-Score:3.47|P-value:2.55E-4||SGD DESC:Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen Gene:PRY2(YKR013W)|FD-Score:3.4|P-value:3.39E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD18(YCR066W)|FD-Score:-3.43|P-value:3.00E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:REC8(YPR007C)|FD-Score:3.27|P-value:5.30E-4||SGD DESC:Meiosis-specific component of sister chromatid cohesion complex; maintains cohesion between sister chromatids during meiosis I; maintains cohesion between centromeres of sister chromatids until meiosis II; homolog of S. pombe Rec8p Gene:REH1(YLR387C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Cytoplasmic 60S subunit biogenesis factor, associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains Gene:RIF2(YLR453C)|FD-Score:4.79|P-value:8.49E-7||SGD DESC:Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation Gene:RIM4(YHL024W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:Putative RNA-binding protein required for the expression of early and middle sporulation genes Gene:RMA1(YKL132C)|FD-Score:5.96|P-value:1.26E-9||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL23B(YER117W)|FD-Score:4.42|P-value:5.01E-6||SGD DESC:Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:4.59|P-value:2.23E-6||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:SFA1(YDL168W)|FD-Score:4.85|P-value:6.08E-7||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SFT2(YBL102W)|FD-Score:-3.99|P-value:3.25E-5||SGD DESC:Non-essential tetra-spanning membrane protein found mostly in the late Golgi, can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5 Gene:SLX8(YER116C)|FD-Score:3.74|P-value:9.11E-5||SGD DESC:Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; stimulated by prior attachment of SUMO to the substrate; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress Gene:SNZ1(YMR096W)|FD-Score:-3.44|P-value:2.88E-4||SGD DESC:Protein involved in vitamin B6 biosynthesis; member of a stationary phase-induced gene family; coregulated with SNO1; interacts with Sno1p and with Yhr198p, perhaps as a multiprotein complex containing other Snz and Sno proteins Gene:SOD1(YJR104C)|FD-Score:-3.43|P-value:3.02E-4||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:STE13(YOR219C)|FD-Score:3.39|P-value:3.48E-4||SGD DESC:Dipeptidyl aminopeptidase, Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor Gene:SWF1(YDR126W)|FD-Score:-3.36|P-value:3.94E-4||SGD DESC:Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion Gene:SYF2(YGR129W)|FD-Score:-3.6|P-value:1.60E-4||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; isy1 syf2 cells have defective spindles activiating cell cycle arrest Gene:TAX4(YJL083W)|FD-Score:-3.35|P-value:4.06E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication Gene:TPK2(YPL203W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase Gene:TRM82(YDR165W)|FD-Score:3.18|P-value:7.43E-4||SGD DESC:Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p Gene:TRP2(YER090W)|FD-Score:-4.96|P-value:3.51E-7||SGD DESC:Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p Gene:UBC7(YMR022W)|FD-Score:8.36|P-value:3.18E-17||SGD DESC:Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly Gene:UBX2(YML013W)|FD-Score:3.49|P-value:2.42E-4||SGD DESC:Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis Gene:VMA16(YHR026W)|FD-Score:-4.06|P-value:2.47E-5||SGD DESC:Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain Gene:VPS55(YJR044C)|FD-Score:-3.33|P-value:4.33E-4||SGD DESC:Late endosomal protein involved in late endosome to vacuole trafficking; functional homolog of human obesity receptor gene-related protein (OB-RGRP) Gene:YAL066W(YAL066W_d)|FD-Score:4.07|P-value:2.40E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAP1(YML007W)|FD-Score:-3.16|P-value:7.92E-4||SGD DESC:Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:YER184C(YER184C_p)|FD-Score:3.78|P-value:7.95E-5||SGD DESC:Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source Gene:YLR278C(YLR278C_p)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene Gene:YME2(YMR302C)|FD-Score:-3.9|P-value:4.78E-5||SGD DESC:Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases Gene:YML018C(YML018C_p)|FD-Score:-5.53|P-value:1.61E-8||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication Gene:YML116W-A(YML116W-A_d)|FD-Score:-3.51|P-value:2.22E-4||SGD DESC:Putative protein of unknown function Gene:YNL097C-B(YNL097C-B_p)|FD-Score:3.21|P-value:6.63E-4||SGD DESC:Putative protein of unknown function Gene:YNL155W(YNL155W_p)|FD-Score:-3.56|P-value:1.89E-4||SGD DESC:Protein of unknown function; contains a DHHC domain and an AN1-type zinc finger; predicted to have thiol-disulfide oxidoreductase active site; transcriptionally regulated by Rpn4p along with proteasome subunit genes; putative ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases in response to DNA replication stress Gene:YOL057W(YOL057W)|FD-Score:-3.22|P-value:6.34E-4||SGD DESC:Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers Gene:YOL114C(YOL114C_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function with similarity to human ICT1 and prokaryotic factors that may function in translation termination; YOL114C is not an essential gene Gene:YPR195C(YPR195C_d)|FD-Score:-3.72|P-value:9.96E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YTA7(YGR270W)|FD-Score:6.21|P-value:2.70E-10||SGD DESC:Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR069C4.751.02E-61.15RRP4Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain RNA binding domains; has similarity to human hRrp4p (EXOSC2)
YGR073C_d4.671.54E-61.15YGR073C_dDubious open reading frame unlikely to encode a functional protein, extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
YDL196W_d4.424.83E-61.15YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YPR085C3.285.28E-40.01ASA1Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
YBR234C3.275.47E-40.24ARC40Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YOR206W3.030.001240.04NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YDL098C2.980.001420.12SNU23Component of U4/U6.U5 snRNP involved in mRNA splicing via spliceosome
YDL087C2.860.002100.01LUC7Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA
YFR002W2.860.002140.03NIC96Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
YDR472W2.820.002380.13TRS31One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
YJR057W2.700.003510.12CDC8Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p
YNL240C2.570.005020.07NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YGR046W2.500.006140.01TAM41Mitochondrial protein required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
YKL021C2.500.006230.00MAK11Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
YDL102W2.500.006280.03POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YMR022W8.363.18E-17UBC7Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
YJL037W7.706.89E-15IRC18Putative protein of unknown function; expression induced in respiratory-deficient cells and in carbon-limited chemostat cultures; similar to adjacent ORF, YJL038C; null mutant displays increased levels of spontaneous Rad52p foci
YKL029C7.154.45E-13MAE1Mitochondrial malic enzyme, catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
YKL168C6.417.40E-11KKQ8Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
YGR270W6.212.70E-10YTA7Protein that localizes to chromatin and has a role in regulation of histone gene expression; has a bromodomain-like region that interacts with the N-terminal tail of histone H3, and an ATPase domain; potentially phosphorylated by Cdc28p
YKL132C5.961.26E-9RMA1Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YLR395C5.305.65E-8COX8Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
YDL168W4.856.08E-7SFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
YLR453C4.798.49E-7RIF2Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
YLR278C_p4.701.28E-6YLR278C_pZinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene
YOR234C4.592.23E-6RPL33BRibosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
YBL021C4.562.53E-6HAP3Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
YGL209W4.532.96E-6MIG2Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication
YER117W4.425.01E-6RPL23BRibosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
YNL168C_p4.231.18E-5FMP41_pPutative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies

GO enrichment analysis for SGTC_2364
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1172.48E-19SGTC_30059076496 71.4 μMChembridge (Drug-like library)213684100.136364
0.0855.35E-11SGTC_23569061756 189.0 μMChembridge (Fragment library)201040430.184615
0.0765.31E-9SGTC_11871391-0724 117.0 μMChemDiv (Drug-like library)69979860.115385amide catabolism
0.0722.87E-8SGTC_23266164455 177.6 μMChembridge (Fragment library)28887900.102941mitochondrial processes
0.0706.55E-8SGTC_4260988-0037 14.6 μMChemDiv (Drug-like library)53830080.0869565ERAD & cell cycle
0.0622.01E-6SGTC_29309013230 14.9 μMChembridge (Drug-like library)29958570.169231heme biosynthesis & mitochondrial translocase
0.0603.57E-6SGTC_590k048-0076 22.4 μMChemDiv (Drug-like library)68328140.0869565RSC & ERG11
0.0604.46E-6SGTC_252miconazole 1.3 nMMiscellaneous41890.142857ergosterol depletion effects on membrane
0.0561.78E-5SGTC_2755lomerizine 42.7 μMMiscellaneous39490.0675676
0.0561.79E-5SGTC_64000l-0018 272.1 μMChemDiv (Drug-like library)68111190.0740741ubiquinone biosynthesis & proteasome
0.0543.53E-5SGTC_6371181-0519 7.3 μMChemDiv (Drug-like library)62520910.15873fatty acid desaturase (OLE1)
0.0534.12E-5SGTC_30999120367 49.5 μMChembridge (Drug-like library)248249400.084507
0.0534.94E-5SGTC_1028k015-0029 4.8 μMChemDiv (Drug-like library)45883090.128571
0.0526.53E-5SGTC_171k064-0041 9.7 μMChemDiv (Drug-like library)533250.140351heme requiring
0.0517.79E-5SGTC_384k007-0144 5.2 μMChemDiv (Drug-like library)68248860.173913ERAD & cell cycle

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21535569715200 μM0.448982054701Chembridge (Fragment library)281.13732.79912iron homeostasis
SGTC_23899091542195.52 μM0.423626624Chembridge (Fragment library)252.719961.88313iron homeostasis
SGTC_3161910038849.47 μM0.39655225236368Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_22917917025104.35 μM0.3859652964612Chembridge (Fragment library)280.321143.03813iron homeostasis
SGTC_22446635614200 μM0.352941836499Chembridge (Fragment library)234.079262.01512
SGTC_3141909460949.47 μM0.3525236309Chembridge (Drug-like library)381.67474.03713iron homeostasis
SGTC_23659042475200 μM0.3454556464479Chembridge (Fragment library)255.314982.56622
SGTC_10553448-067286 μM0.333333748378ChemDiv (Drug-like library)302.369723.92412
SGTC_2254793897595.19 μM0.3272732969277Chembridge (Fragment library)240.752323.75912
SGTC_21485546263113.57 μM0.326923792097Chembridge (Fragment library)216.2541232.76313