9070441

4-[2-(1H-pyridin-2-ylidene)ethylidene]cyclohexa-2,5-dien-1-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2366
Screen concentration 60.6 μM
Source Chembridge (Fragment library)
PubChem CID 6837235
SMILES C1=CC(=CC=C2C=CC(=O)C=C2)NC=C1
Standardized SMILES Oc1ccc(C=Cc2ccccn2)cc1
Molecular weight 197.2325
ALogP 1.1
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.46
% growth inhibition (Hom. pool) 9.67


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 6837235
Download HIP data (tab-delimited text)  (excel)
Gene:ARC19(YKL013C)|FD-Score:3.85|P-value:5.79E-5|Clearance:0.07||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:3.27|P-value:5.34E-4|Clearance:0.3||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BRR6(YGL247W)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CDC31(YOR257W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:MYO2(YOR326W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PGS1(YCL004W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.22||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE10(YOR362C)|FD-Score:5.07|P-value:2.03E-7|Clearance:0.29||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE4(YFR050C)|FD-Score:5.81|P-value:3.16E-9|Clearance:0.74||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PUP3(YER094C)|FD-Score:3.78|P-value:7.71E-5|Clearance:0.29||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPL17A(YKL180W)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.88|P-value:3.07E-12|Clearance:1.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:4.3|P-value:8.60E-6|Clearance:0.44||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TUB1(YML085C)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP20(YBL004W)|FD-Score:4.78|P-value:8.92E-7|Clearance:0.48||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA Gene:ARC19(YKL013C)|FD-Score:3.85|P-value:5.79E-5|Clearance:0.07||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches Gene:ARC35(YNR035C)|FD-Score:3.27|P-value:5.34E-4|Clearance:0.3||SGD DESC:Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin Gene:BRR6(YGL247W)|FD-Score:-3.49|P-value:2.38E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CDC31(YOR257W)|FD-Score:-4.74|P-value:1.07E-6|Clearance:0||SGD DESC:Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation Gene:MYO2(YOR326W)|FD-Score:-3.54|P-value:2.01E-4|Clearance:0||SGD DESC:Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, and the mitotic spindle; MYO2 has a paralog, MYO4, that arose from the whole genome duplication Gene:PGS1(YCL004W)|FD-Score:3.5|P-value:2.36E-4|Clearance:0.22||SGD DESC:Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis Gene:PRE10(YOR362C)|FD-Score:5.07|P-value:2.03E-7|Clearance:0.29||SGD DESC:Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress Gene:PRE4(YFR050C)|FD-Score:5.81|P-value:3.16E-9|Clearance:0.74||SGD DESC:Beta 7 subunit of the 20S proteasome Gene:PUP3(YER094C)|FD-Score:3.78|P-value:7.71E-5|Clearance:0.29||SGD DESC:Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10 Gene:RPL17A(YKL180W)|FD-Score:-3.32|P-value:4.45E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:RPL18A(YOL120C)|FD-Score:-3.11|P-value:9.32E-4|Clearance:0||SGD DESC:Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication Gene:RPP1(YHR062C)|FD-Score:6.88|P-value:3.07E-12|Clearance:1.07||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SEC13(YLR208W)|FD-Score:4.3|P-value:8.60E-6|Clearance:0.44||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:TUB1(YML085C)|FD-Score:-3.53|P-value:2.09E-4|Clearance:0||SGD DESC:Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication Gene:UTP20(YBL004W)|FD-Score:4.78|P-value:8.92E-7|Clearance:0.48||SGD DESC:Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 6837235
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFT1(YGL071W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM9(YER080W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALP1(YNL270C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:ANP1(YEL036C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARG81(YML099C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ART5(YGR068C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:ASN1(YPR145W)|FD-Score:-3.76|P-value:8.53E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC4(YNL269W)|FD-Score:4.76|P-value:9.79E-7||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CAP2(YIL034C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG6(YNL041C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CSM1(YCR086W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAL81(YIR023W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP1(YPL119C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DOG2(YHR043C)|FD-Score:-4.37|P-value:6.08E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:EAF3(YPR023C)|FD-Score:-8.18|P-value:1.37E-16||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:FMP52(YER004W)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR4(YLR449W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GEA1(YJR031C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP4(YAL031C)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:GZF3(YJL110C)|FD-Score:-5.38|P-value:3.78E-8||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAM1(YJR069C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC2(YLR095C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC6(YFR043C)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:9.21|P-value:1.65E-20||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEU9(YOR108W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MED1(YPR070W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MGT1(YDL200C)|FD-Score:5|P-value:2.89E-7||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MKT1(YNL085W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MSI1(YBR195C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MUD1(YBR119W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP1(YGR055W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PCD1(YLR151C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PHO81(YGR233C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMT3(YOR321W)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PSH1(YOL054W)|FD-Score:-4.06|P-value:2.51E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PUS4(YNL292W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD18(YCR066W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMA1(YKL132C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL26A(YLR344W)|FD-Score:-5.26|P-value:7.31E-8||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RTC3(YHR087W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTN2(YDL204W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RUP1(YOR138C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAM2(YDR502C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBP1(YHL034C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEH1(YGL100W)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFP1(YLR403W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHB17(YKR043C)|FD-Score:-3.73|P-value:9.70E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE10(YGL228W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP2(YGL208W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SNF2(YOR290C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SRO9(YCL037C)|FD-Score:5.22|P-value:9.01E-8||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SWD1(YAR003W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWS2(YNL081C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYN8(YAL014C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TIM11(YDR322C-A)|FD-Score:-7.31|P-value:1.35E-13||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TLG2(YOL018C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VHC1(YBR235W_p)|FD-Score:6.38|P-value:9.07E-11||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS25(YJR102C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL065C(YAL065C_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL044W(YBL044W_p)|FD-Score:7|P-value:1.32E-12||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YCL075W(YCL075W)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR016W(YCR016W_p)|FD-Score:-4.52|P-value:3.03E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR101C(YCR101C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL062W(YDL062W_d)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL114W(YDL114W_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YFL051C(YFL051C_p)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIR007W(YIR007W_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YIR042C(YIR042C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL133C(YKL133C_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL053C(YLL053C_p)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR287C(YLR287C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YLR444C(YLR444C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNR064C(YNR064C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR072W(YOR072W_d)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR097C(YOR097C_p)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR314W(YOR314W_d)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.91|P-value:1.72E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR097W(YPR097W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR147C(YPR147C_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS Gene:AAD3(YCR107W)|FD-Score:3.44|P-value:2.88E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFT1(YGL071W)|FD-Score:4.43|P-value:4.74E-6||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:AIM9(YER080W)|FD-Score:3.81|P-value:7.04E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ALD2(YMR170C)|FD-Score:-3.93|P-value:4.20E-5||SGD DESC:Cytoplasmic aldehyde dehydrogenase, involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p Gene:ALP1(YNL270C)|FD-Score:3.17|P-value:7.58E-4||SGD DESC:Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication Gene:ANP1(YEL036C)|FD-Score:5.45|P-value:2.45E-8||SGD DESC:Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol Gene:ARG81(YML099C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Zinc-finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type, involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p Gene:ART5(YGR068C)|FD-Score:3.26|P-value:5.53E-4||SGD DESC:Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane Gene:ASN1(YPR145W)|FD-Score:-3.76|P-value:8.53E-5||SGD DESC:Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication Gene:BFR1(YOR198C)|FD-Score:3.39|P-value:3.54E-4||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BSC4(YNL269W)|FD-Score:4.76|P-value:9.79E-7||SGD DESC:Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p Gene:CAP2(YIL034C)|FD-Score:3.49|P-value:2.43E-4||SGD DESC:Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:4.59|P-value:2.24E-6||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:CHS7(YHR142W)|FD-Score:3.1|P-value:9.63E-4||SGD DESC:Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress Gene:COA1(YIL157C)|FD-Score:3.17|P-value:7.57E-4||SGD DESC:Mitochondrial inner membrane protein required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly Gene:COG6(YNL041C)|FD-Score:3.1|P-value:9.75E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:COQ10(YOL008W)|FD-Score:-3.1|P-value:9.73E-4||SGD DESC:Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes Gene:CSM1(YCR086W)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Nucleolar protein that forms a complex with Lrs4p and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation Gene:DAL81(YIR023W)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism Gene:DBP1(YPL119C)|FD-Score:4.95|P-value:3.72E-7||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress Gene:DOG2(YHR043C)|FD-Score:-4.37|P-value:6.08E-6||SGD DESC:2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, paralogous to DOG1, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed Gene:EAF3(YPR023C)|FD-Score:-8.18|P-value:1.37E-16||SGD DESC:Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition Gene:FMP52(YER004W)|FD-Score:4.28|P-value:9.35E-6||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR4(YLR449W)|FD-Score:3.45|P-value:2.80E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones Gene:GEA1(YJR031C)|FD-Score:-3.12|P-value:8.93E-4||SGD DESC:Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication Gene:GEP4(YHR100C)|FD-Score:5.16|P-value:1.22E-7||SGD DESC:Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT Gene:GIP4(YAL031C)|FD-Score:4.78|P-value:8.72E-7||SGD DESC:Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate Gene:GZF3(YJL110C)|FD-Score:-5.38|P-value:3.78E-8||SGD DESC:GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication Gene:HAM1(YJR069C)|FD-Score:3.34|P-value:4.18E-4||SGD DESC:Nucleoside triphosphate pyrophosphohydrolase; active against a wide range of substrates including ITP, dITP and XTP; mediates exclusion of noncanonical purines and pyrimadines from deoxyribonucleoside triphosphate pools; mutant is sensitive to the base analog 6-N-hydroxylaminopurine; protein abundance increases in response to DNA replication stress Gene:ILM1(YJR118C)|FD-Score:-4.18|P-value:1.48E-5||SGD DESC:Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth Gene:IOC2(YLR095C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif Gene:IRC6(YFR043C)|FD-Score:3.77|P-value:8.24E-5||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci Gene:IRS4(YKR019C)|FD-Score:9.21|P-value:1.65E-20||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:LEU9(YOR108W)|FD-Score:3.43|P-value:3.06E-4||SGD DESC:Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication Gene:LRG1(YDL240W)|FD-Score:3.96|P-value:3.81E-5||SGD DESC:Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis Gene:MED1(YPR070W)|FD-Score:4.66|P-value:1.59E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation Gene:MGT1(YDL200C)|FD-Score:5|P-value:2.89E-7||SGD DESC:DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage Gene:MKT1(YNL085W)|FD-Score:3.59|P-value:1.66E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MSI1(YBR195C)|FD-Score:4.44|P-value:4.49E-6||SGD DESC:Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription; Msi1p localizes to both nucleus and cytoplasm and has an independent role as a negative regulator of the RAS/cAMP pathway via sequestration of Npr1p kinase Gene:MUD1(YBR119W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:U1 snRNP A protein, homolog of human U1-A; involved in nuclear mRNA splicing Gene:MUP1(YGR055W)|FD-Score:-3.6|P-value:1.62E-4||SGD DESC:High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake Gene:PCD1(YLR151C)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member Gene:PHO81(YGR233C)|FD-Score:4.13|P-value:1.78E-5||SGD DESC:Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress Gene:PMT3(YOR321W)|FD-Score:5.12|P-value:1.54E-7||SGD DESC:Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication Gene:POR1(YNL055C)|FD-Score:-3.24|P-value:6.06E-4||SGD DESC:Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication Gene:PSH1(YOL054W)|FD-Score:-4.06|P-value:2.51E-5||SGD DESC:E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p Gene:PUS4(YNL292W)|FD-Score:3.38|P-value:3.61E-4||SGD DESC:Pseudouridine synthase, catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype) Gene:RAD18(YCR066W)|FD-Score:3.41|P-value:3.29E-4||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RAD33(YML011C)|FD-Score:-4.53|P-value:2.90E-6||SGD DESC:Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus Gene:RMA1(YKL132C)|FD-Score:3.63|P-value:1.40E-4||SGD DESC:Putative dihydrofolate synthetase; has similarity to Fol3p and to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:RPL26A(YLR344W)|FD-Score:-5.26|P-value:7.31E-8||SGD DESC:Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication Gene:RPL31B(YLR406C)|FD-Score:-3.62|P-value:1.47E-4||SGD DESC:Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication Gene:RPL9A(YGL147C)|FD-Score:-4.01|P-value:3.02E-5||SGD DESC:Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6 Gene:RTC3(YHR087W)|FD-Score:3.55|P-value:1.95E-4||SGD DESC:Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress Gene:RTF1(YGL244W)|FD-Score:3.16|P-value:8.01E-4||SGD DESC:Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay Gene:RTN2(YDL204W)|FD-Score:3.55|P-value:1.96E-4||SGD DESC:Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; much less abundant than Rtn1p; rtn1 rtn2 yop1 triple mutant lacks tubular ER; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress; RTN2 has a paralog, RTN1, that arose from the whole genome duplication Gene:RUP1(YOR138C)|FD-Score:4.24|P-value:1.13E-5||SGD DESC:Protein that regulates Rsp5p, which is a HECT ubiquitin ligase; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress Gene:RVS161(YCR009C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SAM2(YDR502C)|FD-Score:-3.16|P-value:7.78E-4||SGD DESC:S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM2 has a paralog, SAM1, that arose from the whole genome duplication Gene:SAM37(YMR060C)|FD-Score:3.1|P-value:9.82E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SBP1(YHL034C)|FD-Score:3.27|P-value:5.46E-4||SGD DESC:Protein that binds eIF4G and has a role in repression of translation; has an RGG motif; found in cytoplasmic P bodies; found associated with small nucleolar RNAs snR10 and snR11 Gene:SEH1(YGL100W)|FD-Score:3.91|P-value:4.70E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFP1(YLR403W)|FD-Score:3.84|P-value:6.09E-5||SGD DESC:Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion Gene:SHB17(YKR043C)|FD-Score:-3.73|P-value:9.70E-5||SGD DESC:Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus Gene:SHE10(YGL228W)|FD-Score:3.12|P-value:8.89E-4||SGD DESC:Putative glycosylphosphatidylinositol (GPI)-anchored protein of unknown function; overexpression causes growth arrest Gene:SIP2(YGL208W)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:One of three beta subunits of the Snf1 serine/threonine protein kinase complex involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane Gene:SNF2(YOR290C)|FD-Score:4.56|P-value:2.52E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SRO9(YCL037C)|FD-Score:5.22|P-value:9.01E-8||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.84|P-value:6.16E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SWD1(YAR003W)|FD-Score:3.53|P-value:2.11E-4||SGD DESC:Subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7 Gene:SWS2(YNL081C)|FD-Score:3.29|P-value:4.98E-4||SGD DESC:Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency Gene:SYN8(YAL014C)|FD-Score:4.06|P-value:2.50E-5||SGD DESC:Endosomal SNARE related to mammalian syntaxin 8 Gene:TIM11(YDR322C-A)|FD-Score:-7.31|P-value:1.35E-13||SGD DESC:Subunit e of mitochondrial F1F0-ATPase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase Gene:TLG2(YOL018C)|FD-Score:3.57|P-value:1.81E-4||SGD DESC:Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16) Gene:TOM70(YNL121C)|FD-Score:4.73|P-value:1.12E-6||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TPN1(YGL186C)|FD-Score:3.55|P-value:1.91E-4||SGD DESC:Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p Gene:TPO4(YOR273C)|FD-Score:3.68|P-value:1.15E-4||SGD DESC:Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane Gene:TRP1(YDR007W)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:VHC1(YBR235W_p)|FD-Score:6.38|P-value:9.07E-11||SGD DESC:Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family Gene:VPS25(YJR102C)|FD-Score:-4.13|P-value:1.82E-5||SGD DESC:Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome Gene:VPS61(YDR136C_d)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect Gene:YAL056C-A(YAL056C-A_d)|FD-Score:-3.22|P-value:6.32E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YAL065C(YAL065C_p)|FD-Score:-3.45|P-value:2.81E-4||SGD DESC:Putative protein of unknown function; has homology to FLO1; possible pseudogene Gene:YBL044W(YBL044W_p)|FD-Score:7|P-value:1.32E-12||SGD DESC:Putative protein of unknown function; YBL044W is not an essential protein Gene:YCL075W(YCL075W)|FD-Score:4.87|P-value:5.48E-7||SGD DESC:Pseudogene: encodes fragment of Ty Pol protein Gene:YCR016W(YCR016W_p)|FD-Score:-4.52|P-value:3.03E-6||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus and nucleus; predicted to be involved in ribosome biogenesis Gene:YCR101C(YCR101C_p)|FD-Score:3.69|P-value:1.14E-4||SGD DESC:Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene Gene:YDL041W(YDL041W_d)|FD-Score:3.27|P-value:5.29E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C Gene:YDL062W(YDL062W_d)|FD-Score:-3.2|P-value:6.82E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential Gene:YDL114W(YDL114W_p)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene Gene:YFL051C(YFL051C_p)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative protein of unknown function; YFL051C is not an essential gene Gene:YGR228W(YGR228W_d)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C Gene:YIR007W(YIR007W_p)|FD-Score:5.05|P-value:2.19E-7||SGD DESC:Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene Gene:YIR042C(YIR042C_p)|FD-Score:3.17|P-value:7.75E-4||SGD DESC:Putative protein of unknown function; YIR042C is a non-essential gene Gene:YKL118W(YKL118W_d)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2 Gene:YKL133C(YKL133C_p)|FD-Score:3.23|P-value:6.23E-4||SGD DESC:Putative protein of unknown function; has similarity to Mgr3p, but unlike MGR3, is not required for growth of cells lacking the mitochondrial genome (null mutation does not confer a petite-negative phenotype) Gene:YLL053C(YLL053C_p)|FD-Score:-3.85|P-value:6.00E-5||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YLR287C(YLR287C_p)|FD-Score:4.02|P-value:2.89E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene Gene:YLR444C(YLR444C_d)|FD-Score:4|P-value:3.21E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML009C-A(YML009C-A_d)|FD-Score:-3.27|P-value:5.36E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML012C-A(YML012C-A_d)|FD-Score:3.54|P-value:1.97E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1 Gene:YNR064C(YNR064C)|FD-Score:3.19|P-value:7.20E-4||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:YOR072W(YOR072W_d)|FD-Score:-4.1|P-value:2.11E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive Gene:YOR097C(YOR097C_p)|FD-Score:6.06|P-value:6.62E-10||SGD DESC:Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene Gene:YOR314W(YOR314W_d)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YOR385W(YOR385W_p)|FD-Score:-3.59|P-value:1.65E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene Gene:YPK1(YKL126W)|FD-Score:5.91|P-value:1.72E-9||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR097W(YPR097W)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein that contains a Phox homology (PX) domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YPR147C(YPR147C_p)|FD-Score:-3.14|P-value:8.31E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR062C6.883.07E-121.07RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YFR050C5.813.16E-90.74PRE4Beta 7 subunit of the 20S proteasome
YOR362C5.072.03E-70.29PRE10Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
YBL004W4.788.92E-70.48UTP20Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
YLR208W4.308.60E-60.44SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YKL013C3.855.79E-50.07ARC19Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches
YER094C3.787.71E-50.29PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
YCL004W3.502.36E-40.23PGS1Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
YNR035C3.275.34E-40.30ARC35Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
YLR007W2.970.001460.02NSE1Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
YPR182W2.950.001570.03SMX3Core Sm protein Sm F; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Sme1p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm F
YLR378C2.930.001710.01SEC61Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
YGR047C2.920.001750.01TFC4One of six subunits of the RNA polymerase III transcription initiation factor complex (TFIIIC); part of the TauA domain of TFIIIC that binds BoxA DNA promoter sites of tRNA and similar genes; has TPR motifs; human homolog is TFIIIC-102
YBL023C2.910.001820.01MCM2Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
YPL007C2.900.001870.02TFC8One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; linker between TauB and TauA domains; human homolog is TFIIIC-90

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YKR019C9.211.65E-20IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
YBL044W_p7.001.32E-12YBL044W_pPutative protein of unknown function; YBL044W is not an essential protein
YBR235W_p6.389.07E-11VHC1_pVacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
YOR097C_p6.066.62E-10YOR097C_pPutative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
YKL126W5.911.72E-9YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YEL036C5.452.45E-8ANP1Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
YCL037C5.229.01E-8SRO9Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
YHR100C5.161.22E-7GEP4Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase), dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
YOR321W5.121.54E-7PMT3Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
YIR007W_p5.052.19E-7YIR007W_pPutative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
YDL200C5.002.89E-7MGT1DNA repair methyltransferase (6-O-methylguanine-DNA methylase) involved in protection against DNA alkylation damage
YPL119C4.953.72E-7DBP1Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
YCL075W4.875.48E-7YCL075WPseudogene: encodes fragment of Ty Pol protein
YAL031C4.788.72E-7GIP4Cytoplasmic Glc7-interacting protein whose overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
YNL269W4.769.79E-7BSC4Protein of unknown function, ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p

GO enrichment analysis for SGTC_2366
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1171.72E-19SGTC_2680butyl paraben 22.2 μMMicrosource (Natural product library)71840.173913amide catabolism
0.1163.24E-19SGTC_14740330-0125 5.3 μMChemDiv (Drug-like library)X14740.046875
0.1163.70E-19SGTC_250brefeldin a 552.2 μMICCB bioactive library64361870.046875PDR1
0.1159.94E-19SGTC_1751st044839 27.2 μMTimTec (Natural product derivative library)52913260.0588235Golgi
0.1141.91E-18SGTC_1655st012546 55.1 μMTimTec (Natural product derivative library)7209850.0925926
0.1126.20E-18SGTC_10603448-7350 82.0 μMChemDiv (Drug-like library)5701780.0980392Golgi
0.1127.50E-18SGTC_28729044037 26.0 μMChembridge (Drug-like library)64651950.0555556
0.1093.59E-17SGTC_22557941087 197.4 μMChembridge (Fragment library)9355610.185185
0.1094.87E-17SGTC_7171532-0246 73.2 μMChemDiv (Drug-like library)95619920.133333Golgi
0.1054.91E-16SGTC_24405628896 157.8 μMMiscellaneous55220480.127273
0.1055.11E-16SGTC_6051315-0399 119.0 μMChemDiv (Drug-like library)240204610.0895522Golgi
0.1055.74E-16SGTC_2902043-6722 48.1 μMChemDiv (Drug-like library)7737580.181818
0.1057.87E-16SGTC_1975st070967 30.8 μMTimTec (Natural product derivative library)2613910.0980392
0.1049.78E-16SGTC_8780828-0289 13.7 μMChemDiv (Drug-like library)7453500.0819672
0.1049.90E-16SGTC_6671498-1044 59.0 μMChemDiv (Drug-like library)67399820.145455RPP1 & pyrimidine depletion

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_1087pterostilbene9.05 μM0.3783785281727NIH Clinical Collection256.296443.54113
SGTC_15852',4'-dihydroxychalcone10.4 μM0.3755357218TimTec (Pure natural product library)240.253983.21823
SGTC_25732',4'-dihydroxychalcone15.04 μM0.3755357218TimTec (Pure natural product library)240.253983.21823
SGTC_25742',4'-dihydroxychalcone14.64 μM0.3755357218TimTec (Pure natural product library)240.253983.21823
SGTC_26602',4'-dihydroxychalcone17.14 μM0.3755357218TimTec (Pure natural product library)240.253983.21823
SGTC_26682',4'-dihydroxychalcone15.08 μM0.3755357218TimTec (Pure natural product library)240.253983.21823
SGTC_26044'-hydroxychalcone11.32 μM0.3589745282362Microsource (Natural product library)224.254583.4612
SGTC_1571348-169565.82 μM0.3260875380510ChemDiv (Drug-like library)229.234640.62335iron homeostasis
SGTC_6890833-003998 μM0.3142865374726ChemDiv (Drug-like library)195.259723.15101ergosterol depletion effects on membrane
SGTC_2565isoliquiritigenin100 μM0.309524638278Microsource (Natural product library)256.253382.97534
SGTC_2493resveratrol26.09 μM0.3076926255462Microsource (Natural product library)242.269863.31523
SGTC_9531188-00459.7 μM0.3023266144426ChemDiv (Drug-like library)245.31844.9170160S ribosome export
SGTC_503tyrphostin 8294 μM0.29729792970ICCB bioactive library170.167441.77313TSC3-RPN4
SGTC_3191348-159645.5 μM0.2954555380505ChemDiv (Drug-like library)213.235241.1624RSC & ERG11