9071574

2-propan-2-ylsulfanylaniline hydrochloride

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2371
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 45596039
SMILES CC(C)SC1=CC=CC=C1N.Cl
Standardized SMILES CC(C)Sc1ccccc1N
Molecular weight 203.7322
ALogP 2.73
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 14.82
% growth inhibition (Hom. pool) 6.05


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 45596039
Download HIP data (tab-delimited text)  (excel)
Gene:ARB1(YER036C)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:DCP1(YOL149W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.1||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.35||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:NAR1(YNL240C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.3||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PFY1(YOR122C)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.06||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PTI1(YGR156W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPA43(YOR340C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.01||SGD DESC:RNA polymerase I subunit A43 Gene:SSC1(YJR045C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.35||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:YOR218C(YOR218C_d)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W Gene:ARB1(YER036C)|FD-Score:-3.92|P-value:4.46E-5|Clearance:0||SGD DESC:ATPase of the ATP-binding cassette (ABC) family involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p Gene:DCP1(YOL149W)|FD-Score:3.4|P-value:3.38E-4|Clearance:0.1||SGD DESC:Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress Gene:DED81(YHR019C)|FD-Score:4.07|P-value:2.33E-5|Clearance:0.35||SGD DESC:Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA Gene:NAR1(YNL240C)|FD-Score:3.3|P-value:4.83E-4|Clearance:0.3||SGD DESC:Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf Gene:PFY1(YOR122C)|FD-Score:3.46|P-value:2.66E-4|Clearance:0.06||SGD DESC:Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress Gene:PTI1(YGR156W)|FD-Score:-3.3|P-value:4.83E-4|Clearance:0||SGD DESC:Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64 Gene:RPA43(YOR340C)|FD-Score:3.56|P-value:1.85E-4|Clearance:0.01||SGD DESC:RNA polymerase I subunit A43 Gene:SSC1(YJR045C)|FD-Score:3.91|P-value:4.64E-5|Clearance:0.35||SGD DESC:Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication Gene:YOR218C(YOR218C_d)|FD-Score:3.55|P-value:1.90E-4|Clearance:0.09||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 45596039
Download HOP data (tab-delimited text)  (excel)
Gene:ADY3(YDL239C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AIM3(YBR108W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM46(YHR199C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CLB4(YLR210W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COX11(YPL132W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:DPP1(YDR284C)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DSD1(YGL196W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EMC5(YIL027C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENV9(YOR246C)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GAP1(YKR039W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCY1(YOR120W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX6(YDL010W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GYP8(YFL027C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HEF3(YNL014W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HSP31(YDR533C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IRC4(YDR540C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL13(YGR288W)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MKT1(YNL085W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL38(YKL170W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:OSW2(YLR054C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PDC5(YLR134W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PMP2(YEL017C-A)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RAM1(YDL090C)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV1(YJR033C)|FD-Score:5.81|P-value:3.08E-9||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCY1(YJL204C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RNH202(YDR279W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RNP1(YLL046C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:SPG1(YGR236C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUR7(YML052W)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TDH1(YJL052W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TES1(YJR019C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TMA64(YDR117C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TSC3(YBR058C-A)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR3(YOR006C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:ULI1(YFR026C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:YBL010C(YBL010C_p)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YDR115W(YDR115W_p)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YJL016W(YJL016W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKR033C(YKR033C_d)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL053C(YLL053C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YML090W(YML090W_d)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR007W(YMR007W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YUH1(YJR099W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:ADY3(YDL239C)|FD-Score:3.52|P-value:2.20E-4||SGD DESC:Protein required for spore wall formation; thought to mediate assembly of a Don1p-containing structure at the leading edge of the prospore membrane via interaction with spindle pole body components; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication Gene:AIM3(YBR108W)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Protein interacting with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss Gene:AIM46(YHR199C)|FD-Score:3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss Gene:ARE1(YCR048W)|FD-Score:5.02|P-value:2.61E-7||SGD DESC:Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication Gene:BTS1(YPL069C)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:CLB4(YLR210W)|FD-Score:3.48|P-value:2.47E-4||SGD DESC:B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation Gene:COX11(YPL132W)|FD-Score:-3.42|P-value:3.11E-4||SGD DESC:Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p Gene:DPP1(YDR284C)|FD-Score:4.88|P-value:5.29E-7||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:DSD1(YGL196W)|FD-Score:-3.43|P-value:3.06E-4||SGD DESC:D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate Gene:EMC5(YIL027C)|FD-Score:-3.18|P-value:7.34E-4||SGD DESC:Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT Gene:ENV9(YOR246C)|FD-Score:6.01|P-value:9.18E-10||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts Gene:GAP1(YKR039W)|FD-Score:3.72|P-value:1.01E-4||SGD DESC:General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth Gene:GCY1(YOR120W)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication Gene:GPM3(YOL056W)|FD-Score:3.52|P-value:2.17E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:GRX6(YDL010W)|FD-Score:3.57|P-value:1.75E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:GYP8(YFL027C)|FD-Score:3.37|P-value:3.77E-4||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HEF3(YNL014W)|FD-Score:-3.65|P-value:1.30E-4||SGD DESC:Translational elongation factor EF-3; member of the ABC superfamily; stimulates EF-1 alpha-dependent binding of aminoacyl-tRNA by the ribosome; normally expressed in zinc deficient cells; HEF3 has a paralog, YEF3, that arose from the whole genome duplication Gene:HSP31(YDR533C)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress Gene:IRC4(YDR540C)|FD-Score:-3.38|P-value:3.60E-4||SGD DESC:Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus Gene:IRS4(YKR019C)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication Gene:MAL13(YGR288W)|FD-Score:5.04|P-value:2.28E-7||SGD DESC:MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C Gene:MKT1(YNL085W)|FD-Score:-3.56|P-value:1.88E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRPL38(YKL170W)|FD-Score:-3.42|P-value:3.10E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress Gene:MRPS17(YMR188C)|FD-Score:-3.16|P-value:7.79E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MSH1(YHR120W)|FD-Score:-3.13|P-value:8.65E-4||SGD DESC:DNA-binding protein of the mitochondria involved in repair of mitochondrial DNA, has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis Gene:OSW2(YLR054C)|FD-Score:3.16|P-value:7.99E-4||SGD DESC:Protein of unknown function proposed to be involved in the assembly of the spore wall Gene:PDC5(YLR134W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Minor isoform of pyruvate decarboxylase, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism Gene:PMP2(YEL017C-A)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:POP2(YNR052C)|FD-Score:-3.34|P-value:4.22E-4||SGD DESC:RNase of the DEDD superfamily, subunit of the Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation Gene:RAM1(YDL090C)|FD-Score:-3.33|P-value:4.37E-4||SGD DESC:Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit Gene:RAV1(YJR033C)|FD-Score:5.81|P-value:3.08E-9||SGD DESC:Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate Gene:RCY1(YJL204C)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth Gene:RNH202(YDR279W)|FD-Score:3.21|P-value:6.64E-4||SGD DESC:Ribonuclease H2 subunit, required for RNase H2 activity; related to human AGS2 that causes Aicardi-Goutieres syndrome Gene:RNP1(YLL046C)|FD-Score:3.21|P-value:6.70E-4||SGD DESC:Ribonucleoprotein that contains two RNA recognition motifs (RRM) Gene:SPG1(YGR236C)|FD-Score:3.4|P-value:3.37E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SUR7(YML052W)|FD-Score:4.49|P-value:3.49E-6||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:TDH1(YJL052W)|FD-Score:3.77|P-value:8.17E-5||SGD DESC:Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress Gene:TES1(YJR019C)|FD-Score:3.53|P-value:2.09E-4||SGD DESC:Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids Gene:TMA64(YDR117C)|FD-Score:3.46|P-value:2.66E-4||SGD DESC:Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity Gene:TSC3(YBR058C-A)|FD-Score:4.11|P-value:2.02E-5||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:TSR3(YOR006C)|FD-Score:-3.6|P-value:1.57E-4||SGD DESC:Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress Gene:ULI1(YFR026C)|FD-Score:3.49|P-value:2.40E-4||SGD DESC:Putative protein of unknown function involved in and induced by the endoplasmic reticulum unfolded protein response Gene:YBL010C(YBL010C_p)|FD-Score:3.22|P-value:6.30E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles Gene:YDR115W(YDR115W_p)|FD-Score:4.94|P-value:3.89E-7||SGD DESC:Putative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress Gene:YJL016W(YJL016W_p)|FD-Score:3.19|P-value:7.08E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; conserved in closely related Saccharomyces species Gene:YKR033C(YKR033C_d)|FD-Score:4.37|P-value:6.24E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80 Gene:YLL053C(YLL053C_p)|FD-Score:3.57|P-value:1.78E-4||SGD DESC:Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin Gene:YML090W(YML090W_d)|FD-Score:3.1|P-value:9.68E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR007W(YMR007W_d)|FD-Score:3.71|P-value:1.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YUH1(YJR099W)|FD-Score:3.51|P-value:2.22E-4||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YHR019C4.072.33E-50.35DED81Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA
YJR045C3.914.64E-50.35SSC1Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
YOR340C3.561.85E-40.01RPA43RNA polymerase I subunit A43
YOR218C_d3.551.90E-40.09YOR218C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
YOR122C3.462.66E-40.06PFY1Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress
YOL149W3.403.38E-40.10DCP1Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
YNL240C3.304.83E-40.30NAR1Component of the cytosolic iron-sulfur (FeS) protein assembly machinery, required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; homologous to human Narf
YKL203C3.000.001350.11TOR2PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
YER006W2.890.001950.01NUG1GTPase that associates with nuclear 60S pre-ribosomes, required for export of 60S ribosomal subunits from the nucleus
YKL059C2.880.002020.05MPE1Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif
YGL011C2.830.002340.06SCL1Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
YDR361C2.770.002780.01BCP1Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
YDL196W_d2.760.002860.17YDL196W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31
YLR275W2.590.004804.54E-4SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YER094C2.590.004800.04PUP3Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YOR246C6.019.18E-10ENV9Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
YJR033C5.813.08E-9RAV1Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
YGR288W5.042.28E-7MAL13MAL-activator protein, part of complex locus MAL1; nonfunctional in genomic reference strain S288C
YCR048W5.022.61E-7ARE1Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
YDR115W_p4.943.89E-7YDR115W_pPutative mitochondrial ribosomal protein of the large subunit; similar to E. coli L34 ribosomal protein; required for respiratory growth, as are most mitochondrial ribosomal proteins; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress
YDR284C4.885.29E-7DPP1Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
YML052W4.493.49E-6SUR7Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
YKR033C_d4.376.24E-6YKR033C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80
YJL204C4.221.22E-5RCY1F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
YEL017C-A4.211.29E-5PMP2Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
YBR058C-A4.112.02E-5TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YJL052W3.778.17E-5TDH1Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
YKR039W3.721.01E-4GAP1General amino acid permease; Gap1p senses the presence of amino acid substrates to regulate localization to the plasma membrane when needed; essential for invasive growth
YMR007W_d3.711.02E-4YMR007W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
YKR019C3.611.51E-4IRS4EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2371
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1391.45E-26SGTC_2094077-0173 175.6 μMChemDiv (Drug-like library)159934940.0892857
0.1156.88E-19SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0833333TSC3-RPN4
0.1118.90E-18SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0689655TSC3-RPN4
0.1111.01E-17SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0961538TSC3-RPN4
0.1102.02E-17SGTC_22186965428 193.0 μMChembridge (Fragment library)6756840.06TSC3-RPN4
0.1102.91E-17SGTC_1795st049545 62.3 μMTimTec (Natural product derivative library)52918820.0535714TSC3-RPN4
0.1087.79E-17SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.0952381TSC3-RPN4
0.1062.94E-16SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.102041TSC3-RPN4
0.1056.24E-16SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.0612245TSC3-RPN4
0.1031.81E-15SGTC_320928-0142 34.1 μMChemDiv (Drug-like library)22442420.0909091TSC3-RPN4
0.0952.02E-13SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0526316TSC3-RPN4
0.0937.35E-13SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.111111TSC3-RPN4
0.0911.94E-12SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.0588235TSC3-RPN4
0.0912.24E-12SGTC_8971000-0139 31.4 μMChemDiv (Drug-like library)61510200.152174
0.0912.44E-12SGTC_11730443-0020 122.0 μMChemDiv (Drug-like library)53312950.122449TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_23739071805200 μM0.510149009Chembridge (Fragment library)153.244641.97312
SGTC_23859074692200 μM0.22857117046906Chembridge (Fragment library)201.693142.6422
SGTC_20054024620188 μM0.225236743Chembridge (Fragment library)179.215721.66513
SGTC_1169aniline yellow25.8 μM0.218756051ChemDiv (Drug-like library)197.235843.45213
SGTC_8630466-0191129 μM0.217391765160ChemDiv (Drug-like library)268.267381.67614cell wall
SGTC_20084012253172 μM0.210526603940Chembridge (Fragment library)185.225142.05513
SGTC_21956536160144.24 μM0.2105263834386Chembridge (Fragment library)213.2753.3572260S ribosome export
SGTC_23399000433200 μM0.2093022988954Chembridge (Fragment library)203.237121.95623
SGTC_450aminopyrene156 nM0.20588215352Miscellaneous217.265243.19811
SGTC_3051682-259520.8 μM0.204545226033ChemDiv (Drug-like library)260.311584.35114RNA processing & uracil transport