9072683

6-ethyl-2,4,7-trimethylpurino[7,8-a]imidazole-1,3-dione

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2375
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 16798029
SMILES CCN1C(=CN2C1=NC3=C2C(=O)N(C(=O)N3C)C)C
Standardized SMILES CCn1c(C)cn2c3C(=O)N(C)C(=O)N(C)c3nc12
Molecular weight 261.2798
ALogP 0.55
H-bond donor count 0
H-bond acceptor count 3
Response signature mitochondrial processes

Pool Growth Kinetics
% growth inhibition (Het. pool) 6.13
% growth inhibition (Hom. pool) 3.86


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 16798029
Download HIP data (tab-delimited text)  (excel)
Gene:ACC1(YNR016C)|FD-Score:3.99|P-value:3.30E-5|Clearance:0.17||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.31||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAB4(YGR277C)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.03||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CCT3(YJL014W)|FD-Score:-3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FOL1(YNL256W)|FD-Score:-3.29|P-value:4.92E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GPI18(YBR004C)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.5||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MCM10(YIL150C)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.24||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:PRE3(YJL001W)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPN3(YER021W)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC58(YLR033W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:7.39|P-value:7.44E-14|Clearance:2.61||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:7.19|P-value:3.33E-13|Clearance:2.61||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YDR526C(YDR526C_d)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W Gene:ACC1(YNR016C)|FD-Score:3.99|P-value:3.30E-5|Clearance:0.17||SGD DESC:Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; Gene:ARP4(YJL081C)|FD-Score:3.58|P-value:1.73E-4|Clearance:0.31||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:CAB4(YGR277C)|FD-Score:3.27|P-value:5.38E-4|Clearance:0.03||SGD DESC:Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved Gene:CCT3(YJL014W)|FD-Score:-3.18|P-value:7.26E-4|Clearance:0||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:FOL1(YNL256W)|FD-Score:-3.29|P-value:4.92E-4|Clearance:0||SGD DESC:Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities Gene:GPI18(YBR004C)|FD-Score:4.49|P-value:3.58E-6|Clearance:0.5||SGD DESC:Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria Gene:MCM10(YIL150C)|FD-Score:3.82|P-value:6.67E-5|Clearance:0.24||SGD DESC:Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins Gene:PRE3(YJL001W)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides Gene:RPN3(YER021W)|FD-Score:3.24|P-value:6.05E-4|Clearance:0.13||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control Gene:RSC58(YLR033W)|FD-Score:3.1|P-value:9.57E-4|Clearance:0.1||SGD DESC:Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance Gene:RSC8(YFR037C)|FD-Score:7.39|P-value:7.44E-14|Clearance:2.61||SGD DESC:Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters Gene:RSC9(YML127W)|FD-Score:7.19|P-value:3.33E-13|Clearance:2.61||SGD DESC:Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway Gene:YDR526C(YDR526C_d)|FD-Score:-3.35|P-value:3.97E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YLR198C(YLR198C_d)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.08||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 16798029
Download HOP data (tab-delimited text)  (excel)
Gene:ACB1(YGR037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AGA2(YGL032C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:ALK2(YBL009W)|FD-Score:4.84|P-value:6.59E-7||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:BUD19(YJL188C_d)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CPR8(YNR028W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CYT1(YOR065W)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DAL7(YIR031C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMA1(YHR115C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:ECM29(YHL030W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMP65(YER140W_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR2(YDR519W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GCG1(YER163C_p)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN5(YGR252W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GIP3(YPL137C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HHY1(YEL059W_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:HOS2(YGL194C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:IES1(YFL013C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JIP4(YDR475C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:LEE1(YPL054W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LIA1(YJR070C)|FD-Score:7.61|P-value:1.33E-14||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MDS3(YGL197W)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MID1(YNL291C)|FD-Score:6.41|P-value:7.36E-11||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MIP1(YOR330C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MKT1(YNL085W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRP13(YGR084C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRT4(YKL009W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:NAT4(YMR069W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NRG2(YBR066C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:PBS2(YJL128C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCL10(YGL134W)|FD-Score:3.19|P-value:7.24E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PET191(YJR034W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHM7(YOL084W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:RAD54(YGL163C)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RSF2(YJR127C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEH1(YGL100W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SNF2(YOR290C)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPI1(YER150W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SRB2(YHR041C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO77(YBL106C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSQ1(YLR369W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUR7(YML052W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:THI12(YNL332W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TOD6(YBL054W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRS65(YGR166W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UPS1(YLR193C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VTA1(YLR181C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YEL008W(YEL008W_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YER067C-A(YER067C-A_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Putative protein of unknown function Gene:YHL041W(YHL041W_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR144W(YMR144W_p)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL266W(YNL266W_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YOR022C(YOR022C_p)|FD-Score:5.3|P-value:5.93E-8||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR277C(YOR277C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR343C(YOR343C_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL071C(YPL071C_p)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:ACB1(YGR037C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress Gene:AGA2(YGL032C)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Adhesion subunit of a-agglutinin of a-cells, C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds Gene:ALK2(YBL009W)|FD-Score:4.84|P-value:6.59E-7||SGD DESC:Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins Gene:BUD19(YJL188C_d)|FD-Score:4.23|P-value:1.14E-5||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:CPR8(YNR028W)|FD-Score:4.08|P-value:2.23E-5||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:CYT1(YOR065W)|FD-Score:4.5|P-value:3.42E-6||SGD DESC:Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex Gene:DAL7(YIR031C)|FD-Score:5.19|P-value:1.04E-7||SGD DESC:Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation Gene:DMA1(YHR115C)|FD-Score:-3.11|P-value:9.43E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:ECM29(YHL030W)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:EDS1(YBR033W_p)|FD-Score:3.37|P-value:3.78E-4||SGD DESC:Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication Gene:EMP65(YER140W_p)|FD-Score:4.03|P-value:2.80E-5||SGD DESC:ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:FMP48(YGR052W_p)|FD-Score:-3.36|P-value:3.91E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation Gene:FPR2(YDR519W)|FD-Score:-3.36|P-value:3.84E-4||SGD DESC:Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress Gene:GCG1(YER163C_p)|FD-Score:4.82|P-value:7.20E-7||SGD DESC:Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle Gene:GCN5(YGR252W)|FD-Score:-3.55|P-value:1.93E-4||SGD DESC:Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation Gene:GIP3(YPL137C)|FD-Score:3.88|P-value:5.15E-5||SGD DESC:Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication Gene:HHY1(YEL059W_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein; mutant is hypersensitive to hygromycin B indicative of defects in vacuolar trafficking Gene:HOS2(YGL194C)|FD-Score:3.78|P-value:7.71E-5||SGD DESC:Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS Gene:IES1(YFL013C)|FD-Score:-3.95|P-value:3.96E-5||SGD DESC:Subunit of the INO80 chromatin remodeling complex Gene:JIP4(YDR475C)|FD-Score:3.21|P-value:6.62E-4||SGD DESC:Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence Gene:LEE1(YPL054W)|FD-Score:4.42|P-value:4.87E-6||SGD DESC:Zinc-finger protein of unknown function Gene:LIA1(YJR070C)|FD-Score:7.61|P-value:1.33E-14||SGD DESC:Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress Gene:MDS3(YGL197W)|FD-Score:-3.95|P-value:3.97E-5||SGD DESC:Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain Gene:MID1(YNL291C)|FD-Score:6.41|P-value:7.36E-11||SGD DESC:N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer Gene:MIP1(YOR330C)|FD-Score:3.82|P-value:6.56E-5||SGD DESC:Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit Gene:MKT1(YNL085W)|FD-Score:3.23|P-value:6.09E-4||SGD DESC:Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress Gene:MRP13(YGR084C)|FD-Score:3.26|P-value:5.58E-4||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MRT4(YKL009W)|FD-Score:3.26|P-value:5.63E-4||SGD DESC:Protein involved in mRNA turnover and ribosome assembly, localizes to the nucleolus Gene:NAT4(YMR069W)|FD-Score:-3.35|P-value:4.01E-4||SGD DESC:N alpha-acetyl-transferase, involved in acetylation of the N-terminal residues of histones H4 and H2A Gene:NRG2(YBR066C)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; NRG2 has a paralog, NRG1, that arose from the whole genome duplication Gene:PBS2(YJL128C)|FD-Score:3.56|P-value:1.88E-4||SGD DESC:MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition Gene:PCL10(YGL134W)|FD-Score:3.19|P-value:7.24E-4||SGD DESC:Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate Gene:PET191(YJR034W)|FD-Score:3.18|P-value:7.30E-4||SGD DESC:Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery Gene:PHM7(YOL084W)|FD-Score:3.28|P-value:5.28E-4||SGD DESC:Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress Gene:RAD54(YGL163C)|FD-Score:-4.32|P-value:7.89E-6||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RSF2(YJR127C)|FD-Score:4.11|P-value:1.99E-5||SGD DESC:Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress Gene:SAC3(YDR159W)|FD-Score:3.21|P-value:6.57E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SEH1(YGL100W)|FD-Score:5.11|P-value:1.60E-7||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SNF2(YOR290C)|FD-Score:4.29|P-value:8.86E-6||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SPI1(YER150W)|FD-Score:3.17|P-value:7.59E-4||SGD DESC:GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p Gene:SRB2(YHR041C)|FD-Score:3.15|P-value:8.17E-4||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO77(YBL106C)|FD-Score:3.98|P-value:3.41E-5||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SSQ1(YLR369W)|FD-Score:3.17|P-value:7.60E-4||SGD DESC:Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia Gene:SUR7(YML052W)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:THI12(YNL332W)|FD-Score:3.22|P-value:6.36E-4||SGD DESC:Protein involved in synthesis of the thiamine precursor hydroxymethylpyrimidine (HMP); member of a subtelomeric gene family including THI5, THI11, THI12, and THI13 Gene:TOD6(YBL054W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; TOD6 has a paralog, DOT6, that arose from the whole genome duplication Gene:TRM2(YKR056W)|FD-Score:3.27|P-value:5.32E-4||SGD DESC:tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair Gene:TRS65(YGR166W)|FD-Score:-3.21|P-value:6.61E-4||SGD DESC:Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response Gene:UPS1(YLR193C)|FD-Score:3.49|P-value:2.38E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VTA1(YLR181C)|FD-Score:5.53|P-value:1.56E-8||SGD DESC:Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain Gene:YCL001W-A(YCL001W-A_p)|FD-Score:3.2|P-value:6.81E-4||SGD DESC:Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III Gene:YEL008W(YEL008W_d)|FD-Score:3.17|P-value:7.71E-4||SGD DESC:Hypothetical protein predicted to be involved in metabolism Gene:YER067C-A(YER067C-A_d)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W Gene:YGR035W-A(YGR035W-A_p)|FD-Score:3.8|P-value:7.18E-5||SGD DESC:Putative protein of unknown function Gene:YHL041W(YHL041W_d)|FD-Score:3.17|P-value:7.65E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data Gene:YHR139C-A(YHR139C-A_d)|FD-Score:3.38|P-value:3.58E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YMR144W(YMR144W_p)|FD-Score:5.11|P-value:1.63E-7||SGD DESC:Putative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene Gene:YMR181C(YMR181C_p)|FD-Score:3.17|P-value:7.52E-4||SGD DESC:Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication Gene:YNL266W(YNL266W_d)|FD-Score:3.23|P-value:6.22E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C Gene:YOR022C(YOR022C_p)|FD-Score:5.3|P-value:5.93E-8||SGD DESC:Putative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YOR277C(YOR277C_d)|FD-Score:-3.66|P-value:1.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20 Gene:YOR343C(YOR343C_d)|FD-Score:4.22|P-value:1.20E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YPL071C(YPL071C_p)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YFR037C7.397.44E-142.61RSC8Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
YML127W7.193.33E-132.61RSC9Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
YLR198C_d4.572.41E-60.08YLR198C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
YBR004C4.493.58E-60.50GPI18Functional ortholog of human PIG-V, which is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
YNR016C3.993.30E-50.17ACC1Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
YIL150C3.826.67E-50.24MCM10Essential chromatin-associated protein involved in the initiation of DNA replication; required for the association of the MCM2-7 complex with replication origins
YJL081C3.581.73E-40.31ARP4Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
YGR277C3.275.38E-40.03CAB4Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
YER021W3.246.05E-40.13RPN3Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
YLR033W3.109.57E-40.10RSC58Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
YOR278W3.010.001320.02HEM4Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
YLR323C2.990.001390.08CWC24Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
YDL140C2.910.001810.01RPO21RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
YGL040C2.900.001880.05HEM2Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
YBL105C2.840.002240.03PKC1Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YJR070C7.611.33E-14LIA1Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
YNL291C6.417.36E-11MID1N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
YLR181C5.531.56E-8VTA1Multivesicular body (MVB) protein involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
YOR022C_p5.305.93E-8YOR022C_pPutative carboxylic ester hydrolase; similar to bovine phospholipase A1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YIR031C5.191.04E-7DAL7Malate synthase, role in allantoin degradation unknown; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
YGL100W5.111.60E-7SEH1Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1
YMR144W_p5.111.63E-7YMR144W_pPutative protein of unknown function; localized to the nucleus; YMR144W is not an essential gene
YBL009W4.846.59E-7ALK2Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated in response to DNA damage; contains characteristic motifs for degradation via the APC pathway; ALK2 has a paralog, ALK1, that arose from the whole genome duplication; similar to mammalian haspins
YER163C_p4.827.20E-7GCG1_pGamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
YOR065W4.503.42E-6CYT1Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
YPL054W4.424.87E-6LEE1Zinc-finger protein of unknown function
YOR290C4.298.86E-6SNF2Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
YJL188C_d4.231.14E-5BUD19_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
YOR343C_d4.221.20E-5YOR343C_dDubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
YJR127C4.111.99E-5RSF2Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress

GO enrichment analysis for SGTC_2375
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1908.66E-49SGTC_21866124449 200.0 μMChembridge (Fragment library)38341820.0857143RSC complex & mRNA processing
0.1716.11E-40SGTC_643k048-0106 28.4 μMChemDiv (Drug-like library)67639200.0405405RSC & ERG11
0.1672.66E-38SGTC_1652st011932 78.0 μMTimTec (Natural product derivative library)15510760.0833333RSC complex & mRNA processing
0.1612.96E-35SGTC_22297354935 190.0 μMChembridge (Fragment library)3181120.0793651RSC complex & mRNA processing
0.1525.04E-32SGTC_1651st011928 86.1 μMTimTec (Natural product derivative library)7201980.0285714RSC complex & mRNA processing
0.1461.22E-29SGTC_2534helenine 20.1 μMMicrosource (Natural product library)727240.0704225RSC complex & mRNA processing
0.1448.25E-29SGTC_237nikkomycin 200.0 μMMiscellaneous3534810.0714286RSC complex & mRNA processing
0.1411.26E-27SGTC_23789074213 121.4 μMChembridge (Fragment library)6577140.0677966RSC complex & mRNA processing
0.1373.77E-26SGTC_21095483026 200.0 μMChembridge (Fragment library)7593350.125RSC complex & mRNA processing
0.1323.55E-24SGTC_7093807-1509 243.0 μMChemDiv (Drug-like library)18866770.0555556RSC complex & mRNA processing
0.1315.74E-24SGTC_13742889-5571 141.0 μMChemDiv (Drug-like library)28532810.0547945RSC complex & mRNA processing
0.1286.27E-23SGTC_20415107442 45.3 μMChembridge (Fragment library)7930050.0677966RSC complex & mRNA processing
0.1195.19E-20SGTC_12290139-0193 12.2 μMChemDiv (Drug-like library)30907520.0545455RSC complex & mRNA processing
0.1171.75E-19SGTC_23469031566 143.8 μMChembridge (Fragment library)47710860.196078Golgi
0.1156.84E-19SGTC_322k048-0136 19.3 μMChemDiv (Drug-like library)68232820.0512821RSC & ERG11

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2348902254333.42 μM0.63162678Chembridge (Fragment library)275.306380.8303mitochondrial processes
SGTC_3087911740649.47 μM0.42187542546093Chembridge (Drug-like library)383.831523.45803
SGTC_1740st03828613.22 μM0.403846716786TimTec (Natural product derivative library)266.319461.29213
SGTC_228caffeine993.06 μM0.3913042519Miscellaneous194.1906-0.103
SGTC_1748st03745553.6 μM0.385965580690TimTec (Natural product derivative library)339.176683.02403
SGTC_1676st01848923.4 μM0.3773583696235TimTec (Natural product derivative library)306.403243.5090360S ribosome export
SGTC_1749st03793254.2 μM0.360656594497TimTec (Natural product derivative library)369.202663.33704
SGTC_23387999974200 μM0.358491315925Chembridge (Fragment library)234.254460.96303
SGTC_1791st04831356.1 μM0.3548391204976TimTec (Natural product derivative library)356.37582.86505
SGTC_1736st03677451 μM0.3508771272247TimTec (Natural product derivative library)392.47414.17904