9035213

9,9-dimethyl-10,11-dihydro-8H-benzo[b][1,4]benzodiazepin-7-one

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2379
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 646551
SMILES CC1(CC2=C(C=NC3=CC=CC=C3N2)C(=O)C1)C
Standardized SMILES CC1(C)CC(=O)C2=CNc3ccccc3N=C2C1
Molecular weight 240.3003
ALogP 2.32
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 7
% growth inhibition (Hom. pool) 3.5


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 646551
Download HIP data (tab-delimited text)  (excel)
Gene:BRR6(YGL247W)|FD-Score:-4.48|P-value:3.72E-6|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CAK1(YFL029C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.19||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC11(YJR076C)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC23(YGL128C)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DML1(YMR211W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.18||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:IPI3(YNL182C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:NOC2(YOR206W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP53(YPL146C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RIB3(YDR487C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.18||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RPN6(YDL097C)|FD-Score:-4.83|P-value:6.87E-7|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RRP43(YCR035C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSE1(YML049C)|FD-Score:-3.58|P-value:1.72E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC21(YNL287W)|FD-Score:4.71|P-value:1.25E-6|Clearance:0.43||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SNP1(YIL061C)|FD-Score:-4.35|P-value:6.66E-6|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPC97(YHR172W)|FD-Score:4.12|P-value:1.92E-5|Clearance:0.43||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF12(YDR145W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.02||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TID3(YIL144W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.43||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF35(YDR429C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:WRS1(YOL097C)|FD-Score:4.57|P-value:2.48E-6|Clearance:0.43||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.27|P-value:5.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YHR020W(YHR020W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.43||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments Gene:BRR6(YGL247W)|FD-Score:-4.48|P-value:3.72E-6|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:CAK1(YFL029C)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.19||SGD DESC:Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases Gene:CDC11(YJR076C)|FD-Score:-3.92|P-value:4.38E-5|Clearance:0||SGD DESC:Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate with other rods to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells Gene:CWC23(YGL128C)|FD-Score:-3.33|P-value:4.37E-4|Clearance:0||SGD DESC:Component of a complex containing Cef1p, putatively involved in pre-mRNA splicing; has similarity to E. coli DnaJ and other DnaJ-like proteins and to S. pombe Cwf23p Gene:DML1(YMR211W)|FD-Score:3.67|P-value:1.23E-4|Clearance:0.18||SGD DESC:Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family Gene:IPI3(YNL182C)|FD-Score:3.69|P-value:1.13E-4|Clearance:0.02||SGD DESC:Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene Gene:NOC2(YOR206W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:NOP53(YPL146C)|FD-Score:-3.2|P-value:6.80E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RIB3(YDR487C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.18||SGD DESC:3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration Gene:RPN6(YDL097C)|FD-Score:-4.83|P-value:6.87E-7|Clearance:0||SGD DESC:Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress Gene:RRP43(YCR035C)|FD-Score:-4.14|P-value:1.77E-5|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:RSE1(YML049C)|FD-Score:-3.58|P-value:1.72E-4|Clearance:0||SGD DESC:Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport Gene:SEC21(YNL287W)|FD-Score:4.71|P-value:1.25E-6|Clearance:0.43||SGD DESC:Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo Gene:SNP1(YIL061C)|FD-Score:-4.35|P-value:6.66E-6|Clearance:0||SGD DESC:Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress Gene:SPC97(YHR172W)|FD-Score:4.12|P-value:1.92E-5|Clearance:0.43||SGD DESC:Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque Gene:TAF12(YDR145W)|FD-Score:3.3|P-value:4.90E-4|Clearance:0.02||SGD DESC:Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A Gene:TID3(YIL144W)|FD-Score:4.22|P-value:1.23E-5|Clearance:0.43||SGD DESC:Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering Gene:TIF35(YDR429C)|FD-Score:3.27|P-value:5.39E-4|Clearance:0.04||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:WRS1(YOL097C)|FD-Score:4.57|P-value:2.48E-6|Clearance:0.43||SGD DESC:Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Gene:YGR115C(YGR115C_d)|FD-Score:3.27|P-value:5.32E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YHR020W(YHR020W)|FD-Score:4.16|P-value:1.61E-5|Clearance:0.43||SGD DESC:Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 646551
Download HOP data (tab-delimited text)  (excel)
Gene:ACM1(YPL267W)|FD-Score:4.43|P-value:4.82E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADE4(YMR300C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADK2(YER170W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ATP4(YPL078C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BOR1(YNL275W)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BTS1(YPL069C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:BUD27(YFL023W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCR4(YAL021C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CPR8(YNR028W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:DGR1(YNL130C-A_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPP1(YDR284C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EGD1(YPL037C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ESBP6(YNL125C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FAA2(YER015W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FDC1(YDR539W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FRM2(YCL026C-A)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:GYP8(YFL027C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HIS2(YFR025C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMI1(YOL095C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:KIN3(YAR018C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:LDB16(YCL005W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAG2(YLR427W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MRPL11(YDL202W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL15(YLR312W-A)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL39(YML009C)|FD-Score:-4.79|P-value:8.23E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN2(YMR037C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OAF1(YAL051W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PDX1(YGR193C)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PFS1(YHR185C)|FD-Score:-3.9|P-value:4.91E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PMP2(YEL017C-A)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD18(YCR066W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RBK1(YCR036W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Putative ribokinase Gene:ROX3(YBL093C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPN4(YDL020C)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SEH1(YGL100W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFH5(YJL145W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SIP5(YMR140W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPL2(YHR136C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRO77(YBL106C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK1(YLR006C)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SSO1(YPL232W)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STP1(YDR463W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR7(YML052W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SYM1(YLR251W)|FD-Score:6.88|P-value:3.01E-12||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TAT1(YBR069C)|FD-Score:5.35|P-value:4.37E-8||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TVP23(YDR084C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL012C(YBL012C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W(YBR056W_p)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR178W(YBR178W_d)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YEH1(YLL012W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YGL262W(YGL262W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YHR097C(YHR097C_p)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL102C(YIL102C_p)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function Gene:YIR016W(YIR016W_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication Gene:YLR171W(YLR171W_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML020W(YML020W_p)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function Gene:YMR196W(YMR196W_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function Gene:YNL193W(YNL193W_p)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOL107W(YOL107W_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL136W(YPL136W_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR050C(YPR050C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YUH1(YJR099W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p Gene:ACM1(YPL267W)|FD-Score:4.43|P-value:4.82E-6||SGD DESC:Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein Gene:ADE4(YMR300C)|FD-Score:3.44|P-value:2.91E-4||SGD DESC:Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway Gene:ADK2(YER170W)|FD-Score:4.21|P-value:1.29E-5||SGD DESC:Mitochondrial adenylate kinase, catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background Gene:ATP4(YPL078C)|FD-Score:-3.32|P-value:4.49E-4||SGD DESC:Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex; phosphorylated Gene:BOR1(YNL275W)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1 Gene:BTS1(YPL069C)|FD-Score:3.52|P-value:2.15E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:BUD27(YFL023W)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Unconventional prefoldin protein involved in translation initiation; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; ortholog of human URI/RMP Gene:CCR4(YAL021C)|FD-Score:-3.32|P-value:4.44E-4||SGD DESC:Component of the CCR4-NOT transcriptional complex, which is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening Gene:CPR8(YNR028W)|FD-Score:3.35|P-value:4.08E-4||SGD DESC:Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway Gene:DGR1(YNL130C-A_p)|FD-Score:3.4|P-value:3.43E-4||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DPP1(YDR284C)|FD-Score:-3.29|P-value:5.06E-4||SGD DESC:Diacylglycerol pyrophosphate (DGPP) phosphatase, zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism Gene:EGD1(YPL037C)|FD-Score:-3.25|P-value:5.86E-4||SGD DESC:Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b Gene:ESBP6(YNL125C)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein with similarity to monocarboxylate permeases, appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane Gene:FAA2(YER015W)|FD-Score:3.79|P-value:7.42E-5||SGD DESC:Medium chain fatty acyl-CoA synthetase, activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome Gene:FDC1(YDR539W)|FD-Score:3.95|P-value:3.88E-5||SGD DESC:Putative phenylacrylic acid decarboxylase; essential for the decarboxylation of aromatic carboxylic acids to the corresponding vinyl derivatives; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm Gene:FRM2(YCL026C-A)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin Gene:GYP8(YFL027C)|FD-Score:3.74|P-value:9.04E-5||SGD DESC:GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport Gene:HIS2(YFR025C)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HMI1(YOL095C)|FD-Score:-4.73|P-value:1.13E-6||SGD DESC:Mitochondrial inner membrane localized ATP-dependent DNA helicase, required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD Gene:KIN3(YAR018C)|FD-Score:3.72|P-value:9.77E-5||SGD DESC:Nonessential serine/threonine protein kinase; possible role in DNA damage response Gene:LDB16(YCL005W)|FD-Score:4.44|P-value:4.42E-6||SGD DESC:Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria Gene:MAG2(YLR427W)|FD-Score:-3.36|P-value:3.86E-4||SGD DESC:Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1 Gene:MRPL11(YDL202W)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL15(YLR312W-A)|FD-Score:-3.29|P-value:5.08E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL20(YKR085C)|FD-Score:-3.23|P-value:6.15E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPL39(YML009C)|FD-Score:-4.79|P-value:8.23E-7||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MSN2(YMR037C)|FD-Score:3.16|P-value:7.88E-4||SGD DESC:Transcriptional activator; activated in stress conditions, which results in translocation from the cytoplasm to the nucleus; binds DNA at stress response elements of responsive genes, inducing gene expression; MSN2 has a paralog, MSN4, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:NTH2(YBR001C)|FD-Score:3.43|P-value:2.98E-4||SGD DESC:Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication Gene:OAF1(YAL051W)|FD-Score:3.94|P-value:4.13E-5||SGD DESC:Oleate-activated transcription factor; acts alone and as a heterodimer with Pip2p; activates genes involved in beta-oxidation of fatty acids and peroxisome organization and biogenesis; OAF1 has a paralog, PIP2, that arose from the whole genome duplication Gene:PDX1(YGR193C)|FD-Score:3.72|P-value:9.80E-5||SGD DESC:Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core Gene:PFS1(YHR185C)|FD-Score:-3.9|P-value:4.91E-5||SGD DESC:Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation Gene:PMP2(YEL017C-A)|FD-Score:3.96|P-value:3.82E-5||SGD DESC:Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication Gene:PRY2(YKR013W)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Sterol binding protein involved in the export of acetylated sterols; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins); sterol export function is redundant with that of PRY1; may be involved in detoxification of hydrophobic compounds; PRY2 has a paralog, PRY1, that arose from the whole genome duplication Gene:RAD16(YBR114W)|FD-Score:-3.13|P-value:8.69E-4||SGD DESC:Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex Gene:RAD18(YCR066W)|FD-Score:3.78|P-value:7.80E-5||SGD DESC:E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA Gene:RBK1(YCR036W)|FD-Score:4.03|P-value:2.79E-5||SGD DESC:Putative ribokinase Gene:ROX3(YBL093C)|FD-Score:4.48|P-value:3.74E-6||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme Gene:RPN4(YDL020C)|FD-Score:-4.72|P-value:1.20E-6||SGD DESC:Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress Gene:SAM37(YMR060C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Component of the Sorting and Assembly Machinery (SAM or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability Gene:SEH1(YGL100W)|FD-Score:3.57|P-value:1.80E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFH5(YJL145W)|FD-Score:4.01|P-value:3.04E-5||SGD DESC:Non-classical phosphatidylinositol transfer protein (PITP); exhibits PI- but not PC-transfer activity; localizes to the peripheral endoplasmic reticulum, cytosol and microsomes; similar to Sec14p; partially relocalizes to the plasma membrane upon DNA replication stress Gene:SIP5(YMR140W)|FD-Score:-3.43|P-value:3.05E-4||SGD DESC:Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress Gene:SNX4(YJL036W)|FD-Score:3.25|P-value:5.81E-4||SGD DESC:Sorting nexin, involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p Gene:SPL2(YHR136C)|FD-Score:-3.25|P-value:5.82E-4||SGD DESC:Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm Gene:SRO77(YBL106C)|FD-Score:4.86|P-value:5.92E-7||SGD DESC:Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication Gene:SRV2(YNL138W)|FD-Score:3.82|P-value:6.59E-5||SGD DESC:CAP (cyclase-associated protein) subunit of adenylyl cyclase complex; N-terminus binds adenylyl cyclase and facilitates activation by RAS; C-terminus binds ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis Gene:SSD1(YDR293C)|FD-Score:4.95|P-value:3.80E-7||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SSK1(YLR006C)|FD-Score:-4.32|P-value:7.74E-6||SGD DESC:Cytoplasmic response regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate Gene:SSO1(YPL232W)|FD-Score:4.7|P-value:1.28E-6||SGD DESC:Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p Gene:STP1(YDR463W)|FD-Score:4.44|P-value:4.54E-6||SGD DESC:Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication Gene:SUR7(YML052W)|FD-Score:3.42|P-value:3.12E-4||SGD DESC:Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants Gene:SYM1(YLR251W)|FD-Score:6.88|P-value:3.01E-12||SGD DESC:Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17 Gene:TAT1(YBR069C)|FD-Score:5.35|P-value:4.37E-8||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.98|P-value:3.26E-7||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:TVP23(YDR084C)|FD-Score:-3.25|P-value:5.68E-4||SGD DESC:Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern Gene:YBL012C(YBL012C_d)|FD-Score:3.4|P-value:3.34E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR056W(YBR056W_p)|FD-Score:-3.19|P-value:7.18E-4||SGD DESC:Putative glycoside hydrolase of the mitochondrial intermembrane space Gene:YBR072C-A(YBR072C-A_p)|FD-Score:3.65|P-value:1.34E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBR178W(YBR178W_d)|FD-Score:-3.43|P-value:2.99E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YEH1(YLL012W)|FD-Score:3.78|P-value:7.84E-5||SGD DESC:Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes Gene:YGL262W(YGL262W_p)|FD-Score:3.56|P-value:1.84E-4||SGD DESC:Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene Gene:YHR097C(YHR097C_p)|FD-Score:4.09|P-value:2.18E-5||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus Gene:YIL102C(YIL102C_p)|FD-Score:-3.12|P-value:9.03E-4||SGD DESC:Putative protein of unknown function Gene:YIR016W(YIR016W_p)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication Gene:YLR171W(YLR171W_d)|FD-Score:3.68|P-value:1.17E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YML020W(YML020W_p)|FD-Score:4.36|P-value:6.46E-6||SGD DESC:Putative protein of unknown function Gene:YMR196W(YMR196W_p)|FD-Score:-3.56|P-value:1.83E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene Gene:YMR265C(YMR265C_p)|FD-Score:4.37|P-value:6.29E-6||SGD DESC:Putative protein of unknown function Gene:YNL193W(YNL193W_p)|FD-Score:4.3|P-value:8.67E-6||SGD DESC:Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis Gene:YOL107W(YOL107W_p)|FD-Score:3.41|P-value:3.24E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein Gene:YOR161C-C(YOR161C-C_p)|FD-Score:3.18|P-value:7.47E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YPL136W(YPL136W_d)|FD-Score:-3.5|P-value:2.31E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C Gene:YPR050C(YPR050C_d)|FD-Score:4.21|P-value:1.25E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W Gene:YUH1(YJR099W)|FD-Score:4.5|P-value:3.33E-6||SGD DESC:Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YNL287W4.711.25E-60.43SEC21Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
YOL097C4.572.48E-60.43WRS1Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
YIL144W4.221.23E-50.43TID3Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
YHR020W4.161.61E-50.43YHR020WProlyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
YHR172W4.121.92E-50.43SPC97Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
YNL182C3.691.13E-40.02IPI3Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene
YMR211W3.671.23E-40.18DML1Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
YFL029C3.482.47E-40.19CAK1Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
YDR145W3.304.90E-40.02TAF12Subunit (61/68 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A
YGR115C_d3.275.32E-40.00YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YDR429C3.275.39E-40.04TIF35eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation
YDR487C3.236.20E-40.18RIB33,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase), required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
YCL041C_d3.050.001130.00YCL041C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
YFL037W3.050.001140.07TUB2Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
YPL075W2.980.001440.10GCR1Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR251W6.883.01E-12SYM1Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; homologous to mammalian peroxisomal membrane protein Mpv17
YBR069C5.354.37E-8TAT1Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
YDR007W4.983.26E-7TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YDR293C4.953.80E-7SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YBL106C4.865.92E-7SRO77Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; homolog of Drosophila lethal giant larvae tumor suppressor; interacts with SNARE protein Sec9p; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
YPL232W4.701.28E-6SSO1Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
YJR099W4.503.33E-6YUH1Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
YBL093C4.483.74E-6ROX3Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
YCL005W4.444.42E-6LDB16Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
YDR463W4.444.54E-6STP1Transcription factor; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
YPL267W4.434.82E-6ACM1Pseudosubstrate inhibitor of the anaphase-promoting complex/cyclosome (APC/C), that suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein
YMR265C_p4.376.29E-6YMR265C_pPutative protein of unknown function
YML020W_p4.366.46E-6YML020W_pPutative protein of unknown function
YNL193W_p4.308.67E-6YNL193W_pPutative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
YPR050C_d4.211.25E-5YPR050C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W

GO enrichment analysis for SGTC_2379
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID		 Tanimoto
Score		 Response
Signature
0.1086.90E-17SGTC_23779073351 62.6 μMChembridge (Fragment library)73379460.0909091ergosterol biosynthesis
0.0992.34E-14SGTC_23769072922 81.3 μMChembridge (Fragment library)170879680.0882353
0.0854.87E-11SGTC_1764st044502 93.5 μMTimTec (Natural product derivative library)84070.109091
0.0831.49E-10SGTC_23739071805 200.0 μMChembridge (Fragment library)101490090.117647
0.0741.24E-8SGTC_415dibenzyl ketone 100.0 μMMiscellaneous75930.12766
0.0722.85E-8SGTC_1290088-0017 176.6 μMChemDiv (Drug-like library)1824460.0757576
0.0714.29E-8SGTC_23819035592 200.0 μMChembridge (Fragment library)47496570.116667
0.0707.22E-8SGTC_25005-alpha-androstan-3,17-dione 61.4 μMMicrosource (Natural product library)67106550.107692
0.0672.48E-7SGTC_21375261020 140.0 μMChembridge (Fragment library)7636550.0746269
0.0672.58E-7SGTC_2540epiandrosterone 100.0 μMTimTec (Pure natural product library)4413020.104478
0.0663.72E-7SGTC_15081080-0625 72.9 μMChemDiv (Drug-like library)7671110.0757576
0.0655.93E-7SGTC_8211114-0266 29.2 μMChemDiv (Drug-like library)31232350.109375
0.0656.25E-7SGTC_1472k841-0093 193.0 μMChemDiv (Drug-like library)X14720.0675676
0.0631.22E-6SGTC_33429147791 14.5 μMChembridge (Drug-like library)18381680.0759494
0.0612.25E-6SGTC_1671st016596 74.5 μMTimTec (Natural product derivative library)28291670.08

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count		 H-bond
acceptor
count		 Response
Signature
SGTC_22967971656126.45 μM0.490566666570Chembridge (Fragment library)278.305262.33314
SGTC_10613448-9325110 μM0.321429723482ChemDiv (Drug-like library)277.360263.65812
SGTC_22226885208172.14 μM0.313726609514Chembridge (Fragment library)233.26311.43313
SGTC_235890371574 μM0.2962962388925Chembridge (Fragment library)231.250520.20513
SGTC_1520flindersine88 μM0.27272768230TimTec (Pure natural product library)227.258521.70412
SGTC_3244913448949.47 μM0.23529416584271Chembridge (Drug-like library)340.806783.51604
SGTC_232clozapine119 μM0.2222222818Miscellaneous326.823263.47614NEO1
SGTC_443clozapine122 μM0.2222222818Miscellaneous326.823263.47614NEO1-PIK1
SGTC_14984478-355597.6 μM0.2222223437919ChemDiv (Drug-like library)388.520323.115
SGTC_23286201408200 μM0.2205882890418Chembridge (Fragment library)283.325081.48524
SGTC_22297354935190 μM0.218182318112Chembridge (Fragment library)237.256641.74722RSC complex & mRNA processing