9035592

N-(2-chlorophenyl)-1,1-dioxo-2,3-dihydrothiophen-3-amine

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2381
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 4749657
SMILES C1C(C=CS1(=O)=O)NC2=CC=CC=C2Cl
Standardized SMILES Clc1ccccc1NC2CS(=O)(=O)C=C2
Molecular weight 243.7099
ALogP 1.68
H-bond donor count 1
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.32
% growth inhibition (Hom. pool) -0.02


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 4749657
Download HIP data (tab-delimited text)  (excel)
Gene:BRR6(YGL247W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:BUR6(YER159C)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.15||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:FAL1(YDR021W)|FD-Score:4.17|P-value:1.55E-5|Clearance:0.32||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:GCD7(YLR291C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.25||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NOP53(YPL146C)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RMP1(YLR145W)|FD-Score:3.84|P-value:6.12E-5|Clearance:0.32||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.32||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.32||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.32||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.32||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TOP2(YNL088W)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:UTP21(YLR409C)|FD-Score:4.45|P-value:4.32E-6|Clearance:0.32||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YGL069C(YGL069C_d)|FD-Score:4.04|P-value:2.64E-5|Clearance:0.32||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YLR339C(YLR339C_d)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0 Gene:BRR6(YGL247W)|FD-Score:-3.67|P-value:1.19E-4|Clearance:0||SGD DESC:Essential nuclear envelope integral membrane protein required for nuclear envelope morphology, nuclear pore complex localization, nuclear export; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism Gene:BUR6(YER159C)|FD-Score:3.31|P-value:4.67E-4|Clearance:0.15||SGD DESC:Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha Gene:FAL1(YDR021W)|FD-Score:4.17|P-value:1.55E-5|Clearance:0.32||SGD DESC:Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases Gene:GCD7(YLR291C)|FD-Score:3.16|P-value:7.81E-4|Clearance:0.25||SGD DESC:Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression Gene:NOP53(YPL146C)|FD-Score:-3.15|P-value:8.13E-4|Clearance:0||SGD DESC:Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p; null mutant is viable but growth is severely impaired Gene:RMP1(YLR145W)|FD-Score:3.84|P-value:6.12E-5|Clearance:0.32||SGD DESC:Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P Gene:RPB10(YOR210W)|FD-Score:4.65|P-value:1.68E-6|Clearance:0.32||SGD DESC:RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III Gene:RPB3(YIL021W)|FD-Score:4.52|P-value:3.15E-6|Clearance:0.32||SGD DESC:RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit Gene:RPC53(YDL150W)|FD-Score:3.63|P-value:1.42E-4|Clearance:0.32||SGD DESC:RNA polymerase III subunit C53 Gene:RPP1(YHR062C)|FD-Score:4.57|P-value:2.41E-6|Clearance:0.32||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:SMD2(YLR275W)|FD-Score:4.7|P-value:1.32E-6|Clearance:0.32||SGD DESC:Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2 Gene:TOP2(YNL088W)|FD-Score:-3.53|P-value:2.10E-4|Clearance:0||SGD DESC:Topoisomerase II, relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation Gene:UTP21(YLR409C)|FD-Score:4.45|P-value:4.32E-6|Clearance:0.32||SGD DESC:Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma Gene:YGL069C(YGL069C_d)|FD-Score:4.04|P-value:2.64E-5|Clearance:0.32||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance Gene:YLR339C(YLR339C_d)|FD-Score:3.65|P-value:1.30E-4|Clearance:0.32||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 4749657
Download HOP data (tab-delimited text)  (excel)
Gene:ASP1(YDR321W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:AXL1(YPR122W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BNA3(YJL060W)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BOI2(YER114C)|FD-Score:5.34|P-value:4.66E-8||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CDC50(YCR094W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COX16(YJL003W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CWH43(YCR017C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DOC1(YGL240W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DUN1(YDL101C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM2(YBR065C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERC1(YHR032W)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FCY21(YER060W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAS1(YMR307W)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:HBN1(YCL026C-B_p)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUA2(YOR284W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HUR1(YGL168W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC5(YFR038W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:JHD1(YER051W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:JJJ3(YJR097W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KHA1(YJL094C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MNN2(YBR015C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSD1(YPL104W)|FD-Score:6.52|P-value:3.45E-11||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSO1(YNR049C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC6(YHR151C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NOP12(YOL041C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OSM1(YJR051W)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP12(YOR036W)|FD-Score:5.69|P-value:6.36E-9||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEX22(YAL055W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PPZ1(YML016C)|FD-Score:-4.34|P-value:7.05E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD51(YER095W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RDL1(YOR285W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RHR2(YIL053W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS18B(YML026C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEH1(YGL100W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SMF3(YLR034C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SOD1(YJR104C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPF1(YEL031W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPG1(YGR236C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SSD1(YDR293C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWC3(YAL011W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWI6(YLR182W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TAT1(YBR069C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UGA2(YBR006W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC8(YEL013W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMS1(YDR049W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:WSC2(YNL283C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR012C(YBR012C_d)|FD-Score:-4.91|P-value:4.64E-7||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR290W(YDR290W_d)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER130C(YER130C_p)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER188W(YER188W_d)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR160W(YGR160W_d)|FD-Score:-4.81|P-value:7.44E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR259C(YGR259C_d)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YGR273C(YGR273C_p)|FD-Score:-4.7|P-value:1.32E-6||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR291C(YGR291C_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHK8(YHR048W_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR125W(YLR125W_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YML007C-A(YML007C-A_p)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YOL079W(YOL079W_d)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Putative protein of unknown function Gene:ASP1(YDR321W)|FD-Score:3.57|P-value:1.77E-4||SGD DESC:Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively Gene:AXL1(YPR122W)|FD-Score:-3.75|P-value:8.86E-5||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BNA3(YJL060W)|FD-Score:3.79|P-value:7.51E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BOI2(YER114C)|FD-Score:5.34|P-value:4.66E-8||SGD DESC:Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication Gene:BST1(YFL025C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CDC50(YCR094W)|FD-Score:3.39|P-value:3.47E-4||SGD DESC:Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication Gene:COX16(YJL003W)|FD-Score:-3.47|P-value:2.61E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:CWH43(YCR017C)|FD-Score:3.78|P-value:7.91E-5||SGD DESC:Putative sensor/transporter protein involved in cell wall biogenesis; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion Gene:DOC1(YGL240W)|FD-Score:-3.38|P-value:3.64E-4||SGD DESC:Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC), mediates the activity of the APC by contributing to substrate recognition; involved in cyclin proteolysis; contains a conserved DOC1 homology domain Gene:DUN1(YDL101C)|FD-Score:3.5|P-value:2.33E-4||SGD DESC:Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair Gene:ECM2(YBR065C)|FD-Score:-3.14|P-value:8.35E-4||SGD DESC:Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p Gene:ERC1(YHR032W)|FD-Score:4.35|P-value:6.70E-6||SGD DESC:Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine Gene:FCY21(YER060W)|FD-Score:3.3|P-value:4.89E-4||SGD DESC:Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function Gene:GAS1(YMR307W)|FD-Score:4.28|P-value:9.44E-6||SGD DESC:Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery Gene:HBN1(YCL026C-B_p)|FD-Score:3.28|P-value:5.25E-4||SGD DESC:Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress Gene:HUA2(YOR284W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly Gene:HUR1(YGL168W)|FD-Score:3.32|P-value:4.48E-4||SGD DESC:Protein of unknown function; reported null mutant phenotype of hydroxyurea sensitivity may be due to effects on overlapping PMR1 gene Gene:IRC5(YFR038W)|FD-Score:4.09|P-value:2.11E-5||SGD DESC:Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci Gene:JHD1(YER051W)|FD-Score:-3.39|P-value:3.55E-4||SGD DESC:JmjC domain family histone demethylase specific for H3-K36, similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe Gene:JJJ3(YJR097W)|FD-Score:-3.46|P-value:2.71E-4||SGD DESC:Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4 Gene:KHA1(YJL094C)|FD-Score:-3.32|P-value:4.53E-4||SGD DESC:Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies Gene:MNN2(YBR015C)|FD-Score:4.1|P-value:2.08E-5||SGD DESC:Alpha-1,2-mannosyltransferase, responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment Gene:MSD1(YPL104W)|FD-Score:6.52|P-value:3.45E-11||SGD DESC:Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene Gene:MSO1(YNR049C)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress Gene:MTC6(YHR151C)|FD-Score:-3.21|P-value:6.63E-4||SGD DESC:Protein of unknown function; mtc6 is synthetically sick with cdc13-1 Gene:MTO1(YGL236C)|FD-Score:-4.15|P-value:1.68E-5||SGD DESC:Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants Gene:NOP12(YOL041C)|FD-Score:-3.66|P-value:1.25E-4||SGD DESC:Nucleolar protein involved in pre-25S rRNA processing and biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p Gene:OSM1(YJR051W)|FD-Score:-3.76|P-value:8.47E-5||SGD DESC:Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; mutations cause osmotic sensitivity; OSM1 has a paralog, FRD1, that arose from the whole genome duplication Gene:PCI8(YIL071C)|FD-Score:3.22|P-value:6.50E-4||SGD DESC:Possible shared subunit of Cop9 signalosome (CSN) and eIF3, binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain Gene:PEP12(YOR036W)|FD-Score:5.69|P-value:6.36E-9||SGD DESC:Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin Gene:PEX22(YAL055W)|FD-Score:3.36|P-value:3.83E-4||SGD DESC:Putative peroxisomal membrane protein required for import of peroxisomal proteins, functionally complements a Pichia pastoris pex22 mutation Gene:PPZ1(YML016C)|FD-Score:-4.34|P-value:7.05E-6||SGD DESC:Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance Gene:RAD51(YER095W)|FD-Score:3.61|P-value:1.52E-4||SGD DESC:Strand exchange protein, forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein Gene:RDL1(YOR285W)|FD-Score:5.49|P-value:2.02E-8||SGD DESC:Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress Gene:RHR2(YIL053W)|FD-Score:-3.25|P-value:5.83E-4||SGD DESC:Constitutively expressed glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; RHR2 has a paralog, HOR2, that arose from the whole genome duplication Gene:RPL29(YFR032C-A)|FD-Score:3.16|P-value:7.77E-4||SGD DESC:Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog Gene:RPS18B(YML026C)|FD-Score:3.62|P-value:1.49E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress Gene:SDP1(YIL113W)|FD-Score:3.21|P-value:6.56E-4||SGD DESC:Stress-inducible dual-specificity MAP kinase phosphatase, negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock Gene:SEH1(YGL100W)|FD-Score:4.14|P-value:1.75E-5||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SMF3(YLR034C)|FD-Score:3.45|P-value:2.77E-4||SGD DESC:Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of the Nramp family of metal transport proteins; protein abundance increases in response to DNA replication stress Gene:SOD1(YJR104C)|FD-Score:3.74|P-value:9.31E-5||SGD DESC:Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid Gene:SPF1(YEL031W)|FD-Score:3.27|P-value:5.40E-4||SGD DESC:P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1 Gene:SPG1(YGR236C)|FD-Score:3.66|P-value:1.27E-4||SGD DESC:Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:SSD1(YDR293C)|FD-Score:4.49|P-value:3.60E-6||SGD DESC:Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function Gene:SWC3(YAL011W)|FD-Score:3.83|P-value:6.53E-5||SGD DESC:Protein of unknown function, component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae Gene:SWI6(YLR182W)|FD-Score:3.15|P-value:8.03E-4||SGD DESC:Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators Gene:TAT1(YBR069C)|FD-Score:3.36|P-value:3.91E-4||SGD DESC:Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress Gene:TRP1(YDR007W)|FD-Score:4.2|P-value:1.33E-5||SGD DESC:Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA) Gene:UGA2(YBR006W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm Gene:VAC8(YEL013W)|FD-Score:4.54|P-value:2.83E-6||SGD DESC:Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions Gene:VMS1(YDR049W)|FD-Score:4.42|P-value:4.85E-6||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:WSC2(YNL283C)|FD-Score:3.56|P-value:1.85E-4||SGD DESC:Partially redundant sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway involved in maintenance of cell wall integrity and recovery from heat shock; secretory pathway Wsc2p is required for the arrest of secretion response Gene:YBR012C(YBR012C_d)|FD-Score:-4.91|P-value:4.64E-7||SGD DESC:Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p Gene:YCL001W-B(YCL001W-B_p)|FD-Score:3.57|P-value:1.79E-4||SGD DESC:Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication Gene:YDR290W(YDR290W_d)|FD-Score:-3.26|P-value:5.51E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103 Gene:YER130C(YER130C_p)|FD-Score:5.13|P-value:1.48E-7||SGD DESC:Protein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress Gene:YER188W(YER188W_d)|FD-Score:3.82|P-value:6.72E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR160W(YGR160W_d)|FD-Score:-4.81|P-value:7.44E-7||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR259C(YGR259C_d)|FD-Score:4.15|P-value:1.67E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W Gene:YGR273C(YGR273C_p)|FD-Score:-4.7|P-value:1.32E-6||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YGR291C(YGR291C_d)|FD-Score:3.85|P-value:5.98E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YHK8(YHR048W_p)|FD-Score:3.4|P-value:3.40E-4||SGD DESC:Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles Gene:YHR139C-A(YHR139C-A_d)|FD-Score:-3.16|P-value:8.02E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKL222C(YKL222C)|FD-Score:-3.21|P-value:6.59E-4||SGD DESC:Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine Gene:YLR125W(YLR125W_p)|FD-Score:-3.19|P-value:7.19E-4||SGD DESC:Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene Gene:YLR363W-A(YLR363W-A_p)|FD-Score:-3.62|P-value:1.48E-4||SGD DESC:Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:YML007C-A(YML007C-A_p)|FD-Score:3.25|P-value:5.82E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria Gene:YMR013W-A(YMR013W-A_d)|FD-Score:3.79|P-value:7.61E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 Gene:YOL079W(YOL079W_d)|FD-Score:-3.55|P-value:1.94E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR376W-A(YOR376W-A_p)|FD-Score:4.37|P-value:6.33E-6||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YPK1(YKL126W)|FD-Score:5.06|P-value:2.11E-7||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YPR003C(YPR003C_p)|FD-Score:-3.27|P-value:5.45E-4||SGD DESC:Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene Gene:YPR117W(YPR117W_p)|FD-Score:3.1|P-value:9.61E-4||SGD DESC:Putative protein of unknown function

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR275W4.701.32E-60.32SMD2Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2
YOR210W4.651.68E-60.32RPB10RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III
YHR062C4.572.41E-60.32RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YIL021W4.523.15E-60.32RPB3RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subunit
YLR409C4.454.32E-60.32UTP21Subunit of U3-containing 90S preribosome and Small Subunit (SSU) processome complexes involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma
YDR021W4.171.55E-50.32FAL1Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
YGL069C_d4.042.64E-50.32YGL069C_dDubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
YLR145W3.846.12E-50.32RMP1Subunit of RNase MRP, which processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
YLR339C_d3.651.30E-40.32YLR339C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
YDL150W3.631.42E-40.32RPC53RNA polymerase III subunit C53
YER159C3.314.67E-40.15BUR6Subunit of a heterodimeric NC2 transcription regulator complex with Ncb2p; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha
YLR291C3.167.81E-40.25GCD7Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
YDR224C2.910.001800.06HTB1Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
YNL112W2.850.002170.11DBP2ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
YCL043C2.740.003062.14E-4PDI1Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YPL104W6.523.45E-11MSD1Mitochondrial aspartyl-tRNA synthetase, required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
YOR036W5.696.36E-9PEP12Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
YOR285W5.492.02E-8RDL1Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress
YER114C5.344.66E-8BOI2Protein implicated in polar growth, functionally redundant with Boi1p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
YER130C_p5.131.48E-7YER130C_pProtein of unknown function; transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; computational analysis suggests a role as a transcription factor; C. albicans homolog (MNL1) plays a role in adaptation to stress
YKL126W5.062.11E-7YPK1Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
YEL013W4.542.83E-6VAC8Phosphorylated and palmitoylated vacuolar membrane protein that interacts with Atg13p, required for the cytoplasm-to-vacuole targeting (Cvt) pathway; interacts with Nvj1p to form nucleus-vacuole junctions
YDR293C4.493.60E-6SSD1Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
YDR049W4.424.85E-6VMS1Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
YOR376W-A_p4.376.33E-6YOR376W-A_pPutative protein of unknown function; identified by fungal homology and RT-PCR
YHR032W4.356.70E-6ERC1Member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
YMR307W4.289.44E-6GAS1Beta-1,3-glucanosyltransferase, required for cell wall assembly and also has a role in transcriptional silencing; localizes to the cell surface via a glycosylphosphatidylinositol (GPI) anchor; also found at the nuclear periphery
YBR006W4.241.14E-5UGA2Succinate semialdehyde dehydrogenase involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
YDR007W4.201.33E-5TRP1Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)
YGR259C_d4.151.67E-5YGR259C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W

GO enrichment analysis for SGTC_2381
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1023.27E-15SGTC_1464k208-0027 110.0 μMChemDiv (Drug-like library)X14640.115385
0.1015.69E-15SGTC_2620irigenin 100.0 μMMicrosource (Natural product library)54641700.0441176
0.1001.20E-14SGTC_14113909-8223 150.0 μMChemDiv (Drug-like library)X14110.12
0.0978.01E-14SGTC_29399044578 71.4 μMChembridge (Drug-like library)26536340.157143
0.0952.50E-13SGTC_23539059630 146.7 μMChembridge (Fragment library)47791140.113208
0.0952.40E-13SGTC_14944358-1428 177.0 μMChemDiv (Drug-like library)15645710.08
0.0822.30E-10SGTC_14023909-7673 35.9 μMChemDiv (Drug-like library)53280140.142857
0.0791.11E-9SGTC_2685mycophenolic acid 18.9 μMMiscellaneous4465410.0394737RNA pol III & RNase P/MRP
0.0772.54E-9SGTC_21655727197 32.1 μMChembridge (Fragment library)13617120.0701754
0.0773.21E-9SGTC_14954469-0029 237.0 μMChemDiv (Drug-like library)7936800.09375
0.0757.58E-9SGTC_29389059746 27.3 μMChembridge (Drug-like library)64717690.0694444fatty acid desaturase (OLE1)
0.0758.61E-9SGTC_8360330-0104 1.4 μMChemDiv (Drug-like library)464948200.0892857
0.0758.86E-9SGTC_30859117376 49.5 μMChembridge (Drug-like library)164803490.114286
0.0759.18E-9SGTC_23739071805 200.0 μMChembridge (Fragment library)101490090.106383
0.0741.52E-8SGTC_813k007-0158 173.0 μMChemDiv (Drug-like library)65239160.137931fatty acid desaturase (OLE1)

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_21445628481200 μM0.4651162818399Chembridge (Fragment library)237.3181.99113ERG2
SGTC_3236913270149.47 μM0.31666724282524Chembridge (Drug-like library)378.832.57614TSC3-RPN4
SGTC_21015377231179.23 μM0.32844213Chembridge (Fragment library)256.708662.35514
SGTC_20635246145200 μM0.297872711240Chembridge (Fragment library)221.639722.67412
SGTC_2895905444758.44 μM0.2830192697450Chembridge (Drug-like library)343.850483.16113
SGTC_1300136-0219337.66 μM0.28234244ChemDiv (Drug-like library)296.148643.77812
SGTC_2994905352071.43 μM0.2777784647384Chembridge (Drug-like library)300.782683.27912RPP1 & pyrimidine depletion
SGTC_74000s-0487160.28 μM0.269231677548ChemDiv (Drug-like library)277.702982.87223
SGTC_8201582-006059.9 μM0.2592593848840ChemDiv (Drug-like library)290.661983.50816
SGTC_2841900476138.96 μM0.252991039Chembridge (Drug-like library)289.756744.08412amide catabolism