9036125

4-(3-methoxyphenyl)-1-methylpiperidine-4-carbonitrile

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2383
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 79580
SMILES CN1CCC(CC1)(C#N)C2=CC(=CC=C2)OC
Standardized SMILES COc1cccc(c1)C2(CCN(C)CC2)C#N
Molecular weight 230.3055
ALogP 2.1
H-bond donor count 0
H-bond acceptor count 3
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 2.45
% growth inhibition (Hom. pool) -1.25


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 79580
Download HIP data (tab-delimited text)  (excel)
Gene:BMS1(YPL217C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.13||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CHS2(YBR038W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.63||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:ESA1(YOR244W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:ESP1(YGR098C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KRE29(YER038C)|FD-Score:-4.28|P-value:9.35E-6|Clearance:0||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MPE1(YKL059C)|FD-Score:-3.16|P-value:7.86E-4|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOB1(YOR056C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.19||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-3.98|P-value:3.44E-5|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:SPC34(YKR037C)|FD-Score:4.44|P-value:4.51E-6|Clearance:0.63||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:TAF3(YPL011C)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TEL2(YGR099W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.09||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1 Gene:BMS1(YPL217C)|FD-Score:3.5|P-value:2.34E-4|Clearance:0.13||SGD DESC:GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p Gene:CHS2(YBR038W)|FD-Score:4.12|P-value:1.86E-5|Clearance:0.63||SGD DESC:Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis Gene:ESA1(YOR244W)|FD-Score:-3.5|P-value:2.31E-4|Clearance:0||SGD DESC:Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy Gene:ESP1(YGR098C)|FD-Score:-3.32|P-value:4.53E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:KRE29(YER038C)|FD-Score:-4.28|P-value:9.35E-6|Clearance:0||SGD DESC:Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance Gene:MPE1(YKL059C)|FD-Score:-3.16|P-value:7.86E-4|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:NOB1(YOR056C)|FD-Score:3.37|P-value:3.80E-4|Clearance:0.19||SGD DESC:Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress Gene:PRE6(YOL038W)|FD-Score:-3.98|P-value:3.44E-5|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:SPC34(YKR037C)|FD-Score:4.44|P-value:4.51E-6|Clearance:0.63||SGD DESC:Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body Gene:TAF3(YPL011C)|FD-Score:-3.19|P-value:7.07E-4|Clearance:0||SGD DESC:TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation Gene:TEL2(YGR099W)|FD-Score:3.18|P-value:7.40E-4|Clearance:0.09||SGD DESC:Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 79580
Download HOP data (tab-delimited text)  (excel)
Gene:ADY2(YCR010C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD5(YER073W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ANS1(YHR126C_p)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ATO3(YDR384C)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:COX16(YJL003W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:DMA1(YHR115C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DTD1(YDL219W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:FIG1(YBR040W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FRE7(YOL152W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GIS2(YNL255C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GPM3(YOL056W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:ICY2(YPL250C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LSB3(YFR024C-A)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:NGG1(YDR176W)|FD-Score:4.87|P-value:5.57E-7||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NGL3(YML118W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NGR1(YBR212W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NPP1(YCR026C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NTG2(YOL043C)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PNG1(YPL096W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD54(YGL163C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SKN1(YGR143W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:TOK1(YJL093C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TOM6(YOR045W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:YBR178W(YBR178W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YEL023C(YEL023C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:-3.82|P-value:6.79E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YER188W(YER188W_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR021W(YGR021W_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR069W(YGR069W_d)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJR011C(YJR011C_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR063W(YLR063W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR253W(YLR253W_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR271W(YLR271W_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNR064C(YNR064C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides Gene:ADY2(YCR010C)|FD-Score:4.07|P-value:2.33E-5||SGD DESC:Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication Gene:AFT1(YGL071W)|FD-Score:3.29|P-value:4.94E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ALD5(YER073W)|FD-Score:3.47|P-value:2.60E-4||SGD DESC:Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed Gene:ANS1(YHR126C_p)|FD-Score:4.41|P-value:5.14E-6||SGD DESC:Putative GPI protein; transcription dependent upon Azf1p Gene:ATO3(YDR384C)|FD-Score:3.79|P-value:7.53E-5||SGD DESC:Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters Gene:COX16(YJL003W)|FD-Score:-3.3|P-value:4.80E-4||SGD DESC:Mitochondrial inner membrane protein, required for assembly of cytochrome c oxidase Gene:DMA1(YHR115C)|FD-Score:-3.21|P-value:6.65E-4||SGD DESC:Ubiquitin-protein ligase (E3); controls septin dynamics and the spindle position checkpoint (SPOC) along with functionally redundant ligase Dma2p by regulating the recruitment of Elm1p to the bud neck; regulates levels of the translation initiation factor eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8 protein, similar to human Chfr; contains FHA and RING finger Gene:DTD1(YDL219W)|FD-Score:-3.15|P-value:8.16E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:FIG1(YBR040W)|FD-Score:3.2|P-value:6.85E-4||SGD DESC:Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating Gene:FRE7(YOL152W)|FD-Score:3.51|P-value:2.21E-4||SGD DESC:Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels Gene:GIS2(YNL255C)|FD-Score:-3.46|P-value:2.75E-4||SGD DESC:Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2 Gene:GPM3(YOL056W)|FD-Score:3.35|P-value:4.07E-4||SGD DESC:Homolog of Gpm1p phosphoglycerate mutase, which converts 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; may be non-functional derivative of a gene duplication event Gene:ICY2(YPL250C)|FD-Score:-3.35|P-value:4.10E-4||SGD DESC:Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate Gene:LSB3(YFR024C-A)|FD-Score:-4.6|P-value:2.09E-6||SGD DESC:Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication Gene:MIG3(YER028C)|FD-Score:-3.36|P-value:3.88E-4||SGD DESC:Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication Gene:MMR1(YLR190W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Phosphorylated protein of the mitochondrial outer membrane, localizes only to mitochondria of the bud; interacts with Myo2p to mediate mitochondrial distribution to buds; mRNA is targeted to the bud via the transport system involving She2p Gene:NGG1(YDR176W)|FD-Score:4.87|P-value:5.57E-7||SGD DESC:Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex Gene:NGL3(YML118W)|FD-Score:3.59|P-value:1.65E-4||SGD DESC:3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication Gene:NGR1(YBR212W)|FD-Score:-3.51|P-value:2.26E-4||SGD DESC:RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase Gene:NPP1(YCR026C)|FD-Score:3.37|P-value:3.75E-4||SGD DESC:Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication Gene:NTG2(YOL043C)|FD-Score:-5.08|P-value:1.86E-7||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:PNG1(YPL096W)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p Gene:PPT2(YPL148C)|FD-Score:3.34|P-value:4.14E-4||SGD DESC:Phosphopantetheine:protein transferase (PPTase), activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation Gene:RAD54(YGL163C)|FD-Score:4.87|P-value:5.63E-7||SGD DESC:DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress Gene:RTC4(YNL254C)|FD-Score:-4.1|P-value:2.07E-5||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SKN1(YGR143W)|FD-Score:-3.67|P-value:1.19E-4||SGD DESC:Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p Gene:TOK1(YJL093C)|FD-Score:-4.08|P-value:2.23E-5||SGD DESC:Outward-rectifier potassium channel of the plasma membrane with two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin Gene:TOM6(YOR045W)|FD-Score:3.4|P-value:3.42E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex Gene:YBR178W(YBR178W_d)|FD-Score:3.28|P-value:5.27E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C Gene:YEL023C(YEL023C_p)|FD-Score:3.47|P-value:2.57E-4||SGD DESC:Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene Gene:YER156C(YER156C_p)|FD-Score:-3.82|P-value:6.79E-5||SGD DESC:Putative protein of unknown function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; similarity to mammalian MYG1 Gene:YER188W(YER188W_d)|FD-Score:3.36|P-value:3.92E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p Gene:YGR021W(YGR021W_p)|FD-Score:4.08|P-value:2.22E-5||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:YGR069W(YGR069W_d)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YGR283C(YGR283C)|FD-Score:3.59|P-value:1.64E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus Gene:YJR011C(YJR011C_p)|FD-Score:-3.14|P-value:8.59E-4||SGD DESC:Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS Gene:YLR063W(YLR063W_p)|FD-Score:-3.51|P-value:2.25E-4||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene Gene:YLR253W(YLR253W_p)|FD-Score:3.23|P-value:6.20E-4||SGD DESC:Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene Gene:YLR271W(YLR271W_p)|FD-Score:3.28|P-value:5.26E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS Gene:YNR064C(YNR064C)|FD-Score:5.24|P-value:8.16E-8||SGD DESC:Epoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YKR037C4.444.51E-60.63SPC34Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
YBR038W4.121.86E-50.63CHS2Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
YPL217C3.502.34E-40.13BMS1GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
YOR056C3.373.80E-40.19NOB1Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
YGR099W3.187.40E-40.09TEL2Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
YHR148W3.080.001020.09IMP3Component of the SSU processome, which is required for pre-18S rRNA processing, essential protein that interacts with Mpp10p and mediates interactions of Imp4p and Mpp10p with U3 snoRNA
YGL068W2.990.001390.10MNP1Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
YBR143C2.890.001960.08SUP45Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
YNL267W2.810.002510.01PIK1Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
YGR030C2.800.002570.03POP6Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YGL111W2.770.002810.06NSA1Constituent of 66S pre-ribosomal particles, involved in 60S ribosomal subunit biogenesis
YPL016W2.710.003360.06SWI1Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; can form the prion [SWI+]; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
YKR071C2.650.004080.05DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YGL120C2.600.004650.02PRP43RNA helicase in the DEAH-box family, functions in both RNA polymerase I and polymerase II transcript metabolism, involved in release of the lariat-intron from the spliceosome
YGL103W2.580.004940.02RPL28Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YNR064C5.248.16E-8YNR064CEpoxide hydrolase, member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
YDR176W4.875.57E-7NGG1Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
YGL163C4.875.63E-7RAD54DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
YHR126C_p4.415.14E-6ANS1_pPutative GPI protein; transcription dependent upon Azf1p
YGR021W_p4.082.22E-5YGR021W_pPutative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YCR010C4.072.33E-5ADY2Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
YDR384C3.797.53E-5ATO3Plasma membrane protein, regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
YGR283C3.591.64E-4YGR283CPutative S-adenosylmethionine-dependent methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus
YPL096W3.591.64E-4PNG1Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p
YML118W3.591.65E-4NGL33'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
YOL152W3.512.21E-4FRE7Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels
YEL023C_p3.472.57E-4YEL023C_pPutative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
YER073W3.472.60E-4ALD5Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
YOR045W3.403.42E-4TOM6Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
YCR026C3.373.75E-4NPP1Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; NPP1 has a paralog, NPP2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2383
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1632.47E-36SGTC_23869075165 157.4 μMChembridge (Fragment library)164522770.0793651
0.1254.66E-22SGTC_23889080168 200.0 μMChembridge (Fragment library)159430730.0847458
0.0983.49E-14SGTC_23729071633 200.0 μMChembridge (Fragment library)213213660.109091
0.0912.32E-12SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.103448
0.0857.30E-11SGTC_222nsc-310342 10.7 μMMiscellaneous4325560.0441176
0.0807.03E-10SGTC_224benfluorex 43.9 μMMiscellaneous23180.109589
0.0791.40E-9SGTC_225nsc-122657 1.0 μMMiscellaneous2756850.078125
0.0764.67E-9SGTC_220paclitaxel 373.4 μMMiscellaneous46660.0701754
0.0757.90E-9SGTC_1703st026983 48.3 μMTimTec (Natural product derivative library)11040230.0714286
0.0691.09E-7SGTC_9861493-0483 84.0 μMChemDiv (Drug-like library)400790.112903
0.0672.20E-7SGTC_820054-0107 293.8 μMChemDiv (Drug-like library)31046970.0923077
0.0649.91E-7SGTC_448monastrol 171.0 μMICCB bioactive library29879270.111111
0.0603.29E-6SGTC_223busulfan 5.0 mMMiscellaneous24780.0416667
0.0589.13E-6SGTC_9963970-0799 1.8 μMChemDiv (Drug-like library)66156480.109375
0.0588.06E-6SGTC_14394092-1168 46.0 μMChemDiv (Drug-like library)28862400.266667

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_8530419-030621 μM0.416667762713ChemDiv (Drug-like library)268.35024.54512
SGTC_20885278280127.39 μM0.320755791502Chembridge (Fragment library)284.336281.44726
SGTC_20214019055173 μM0.312544827708Chembridge (Fragment library)163.216321.26112endomembrane recycling
SGTC_3081911724349.47 μM0.30357145595589Chembridge (Drug-like library)299.793082.47314fatty acid desaturase (OLE1)
SGTC_13752910-0797186 μM0.293103742508ChemDiv (Drug-like library)340.416162.75204
SGTC_14394092-116846 μM0.2666672886240ChemDiv (Drug-like library)346.851123.8514
SGTC_20185133068143 μM0.259259785362Chembridge (Fragment library)186.20992.0212
SGTC_23207373003200 μM0.2580655415828Chembridge (Fragment library)291.325621.29425
SGTC_12560570-0116285 μM0.254902410638ChemDiv (Drug-like library)280.362661.81105
SGTC_5544483-2211187 μM0.25747107ChemDiv (Drug-like library)285.29462.45314