9080168

2-(2,2-dimethylpropanoylamino)-N-methylbenzamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2388
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 15943073
SMILES CC(C)(C)C(=O)NC1=CC=CC=C1C(=O)NC
Standardized SMILES CNC(=O)c1ccccc1NC(=O)C(C)(C)C
Molecular weight 234.2942
ALogP 1.7
H-bond donor count 2
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 3.68
% growth inhibition (Hom. pool) 1.24


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 15943073
Download HIP data (tab-delimited text)  (excel)
Gene:ERV1(YGR029W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.24||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:ESP1(YGR098C)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:MPE1(YKL059C)|FD-Score:-3.93|P-value:4.27E-5|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:ORC3(YLL004W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POL3(YDL102W)|FD-Score:4.79|P-value:8.14E-7|Clearance:0.76||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE6(YOL038W)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RNA1(YMR235C)|FD-Score:-3.75|P-value:8.80E-5|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:SFH1(YLR321C)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:YDL016C(YDL016C_d)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.76||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication Gene:ERV1(YGR029W)|FD-Score:3.44|P-value:2.92E-4|Clearance:0.24||SGD DESC:Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR) Gene:ESP1(YGR098C)|FD-Score:-3.24|P-value:5.92E-4|Clearance:0||SGD DESC:Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress Gene:MPE1(YKL059C)|FD-Score:-3.93|P-value:4.27E-5|Clearance:0||SGD DESC:Essential conserved subunit of CPF (cleavage and polyadenylation factor), plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA, contains a putative RNA-binding zinc knuckle motif Gene:ORC3(YLL004W)|FD-Score:-3.42|P-value:3.11E-4|Clearance:0||SGD DESC:Subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing Gene:POL3(YDL102W)|FD-Score:4.79|P-value:8.14E-7|Clearance:0.76||SGD DESC:Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) Gene:PRE6(YOL038W)|FD-Score:-3.48|P-value:2.55E-4|Clearance:0||SGD DESC:Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress Gene:RNA1(YMR235C)|FD-Score:-3.75|P-value:8.80E-5|Clearance:0||SGD DESC:GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport Gene:SFH1(YLR321C)|FD-Score:3.2|P-value:6.89E-4|Clearance:0.17||SGD DESC:Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog Gene:YDL016C(YDL016C_d)|FD-Score:4.2|P-value:1.35E-5|Clearance:0.76||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 15943073
Download HOP data (tab-delimited text)  (excel)
Gene:ADE3(YGR204W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AXL1(YPR122W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD19(YJL188C_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CYK3(YDL117W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DTD1(YDL219W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:GDA1(YEL042W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GRE3(YHR104W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HSP150(YJL159W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:HSP30(YCR021C)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IPK1(YDR315C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:KRE6(YPR159W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MRI1(YPR118W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NCE101(YJL205C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NOT3(YIL038C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NTG2(YOL043C)|FD-Score:-4.46|P-value:4.09E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PPQ1(YPL179W)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RDR1(YOR380W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RKM2(YDR198C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RRT2(YBR246W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTC4(YNL254C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SEH1(YGL100W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFA1(YDL168W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHM2(YLR058C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIP3(YNL257C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLX9(YGR081C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF4(YGL115W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TED1(YIL039W)|FD-Score:-4.4|P-value:5.33E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRX3(YCR083W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAB2(YEL005C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VMS1(YDR049W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YCL002C(YCL002C_p)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR006C(YCR006C_d)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YML090W(YML090W_d)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR1(YJR110W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YOR121C(YOR121C_d)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPR098C(YPR098C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane Gene:ADE3(YGR204W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine Gene:AXL1(YPR122W)|FD-Score:3.45|P-value:2.82E-4||SGD DESC:Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells Gene:BCK1(YJL095W)|FD-Score:-3.56|P-value:1.87E-4||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BUD19(YJL188C_d)|FD-Score:3.28|P-value:5.12E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUL1(YMR275C)|FD-Score:3.35|P-value:3.99E-4||SGD DESC:Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication Gene:CYK3(YDL117W)|FD-Score:3.45|P-value:2.85E-4||SGD DESC:SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants Gene:DTD1(YDL219W)|FD-Score:-3.51|P-value:2.23E-4||SGD DESC:D-Tyr-tRNA(Tyr) deacylase, functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes Gene:GDA1(YEL042W)|FD-Score:-3.23|P-value:6.18E-4||SGD DESC:Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate Gene:GRE3(YHR104W)|FD-Score:3.23|P-value:6.27E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:HSP150(YJL159W)|FD-Score:-3.32|P-value:4.47E-4||SGD DESC:O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication Gene:HSP30(YCR021C)|FD-Score:-4.46|P-value:4.10E-6||SGD DESC:Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase Gene:IPK1(YDR315C)|FD-Score:3.3|P-value:4.76E-4||SGD DESC:Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable Gene:KRE6(YPR159W)|FD-Score:3.13|P-value:8.86E-4||SGD DESC:Type II integral membrane protein required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to the ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p Gene:MRI1(YPR118W)|FD-Score:3.37|P-value:3.76E-4||SGD DESC:5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway Gene:NCE101(YJL205C)|FD-Score:3.44|P-value:2.96E-4||SGD DESC:Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences Gene:NOT3(YIL038C)|FD-Score:-3.19|P-value:7.03E-4||SGD DESC:Subunit of the CCR4-NOT complex, which is a global transcriptional regulator with roles in transcription initiation and elongation and in mRNA degradation Gene:NTG2(YOL043C)|FD-Score:-4.46|P-value:4.09E-6||SGD DESC:DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication Gene:OYE3(YPL171C)|FD-Score:-3.7|P-value:1.08E-4||SGD DESC:Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN), homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death Gene:PPQ1(YPL179W)|FD-Score:3.83|P-value:6.28E-5||SGD DESC:Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors Gene:PUF4(YGL014W)|FD-Score:3.61|P-value:1.51E-4||SGD DESC:Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors Gene:RDR1(YOR380W)|FD-Score:-3.4|P-value:3.38E-4||SGD DESC:Transcriptional repressor involved in the control of multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins Gene:RKM2(YDR198C)|FD-Score:3.99|P-value:3.37E-5||SGD DESC:Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp Gene:RRT2(YBR246W)|FD-Score:3.46|P-value:2.67E-4||SGD DESC:Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription Gene:RTC4(YNL254C)|FD-Score:-3.46|P-value:2.67E-4||SGD DESC:Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus Gene:SEH1(YGL100W)|FD-Score:-3.68|P-value:1.18E-4||SGD DESC:Component of two distinct complexes; subunit of the Nup84 nuclear pore sub-complex (NPC) and the Seh1-associated (SEA) complex; the the NUP84 subcomplex contributes to nucleocytoplasmic transport and NPC biogenesis; the SEA complex is a coatamer-related complex that associates dynamically with the vacuole; homologous to human SEH1 Gene:SFA1(YDL168W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress Gene:SHM2(YLR058C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis Gene:SIP3(YNL257C)|FD-Score:3.16|P-value:7.79E-4||SGD DESC:Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication Gene:SLX9(YGR081C)|FD-Score:3.86|P-value:5.60E-5||SGD DESC:Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant Gene:SNF4(YGL115W)|FD-Score:3.34|P-value:4.21E-4||SGD DESC:Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress Gene:TCA17(YEL048C)|FD-Score:-3.44|P-value:2.96E-4||SGD DESC:Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder Gene:TED1(YIL039W)|FD-Score:-4.4|P-value:5.33E-6||SGD DESC:Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) Gene:TRX3(YCR083W)|FD-Score:-3.45|P-value:2.76E-4||SGD DESC:Mitochondrial thioredoxin, highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p Gene:VAB2(YEL005C)|FD-Score:3.19|P-value:7.05E-4||SGD DESC:Protein with a potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of BLOC complex involved in endosomal cargo sorting; Vab2p-GFP-fusion localizes to cytoplasm in punctate patter Gene:VMS1(YDR049W)|FD-Score:3.38|P-value:3.57E-4||SGD DESC:Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans Gene:YCL002C(YCL002C_p)|FD-Score:3.87|P-value:5.48E-5||SGD DESC:Putative protein of unknown function; YCL002C is not an essential gene Gene:YCR006C(YCR006C_d)|FD-Score:3.17|P-value:7.72E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YER121W(YER121W_p)|FD-Score:3.54|P-value:2.01E-4||SGD DESC:Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy Gene:YML090W(YML090W_d)|FD-Score:-3.53|P-value:2.05E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source Gene:YMR1(YJR110W)|FD-Score:3.14|P-value:8.38E-4||SGD DESC:Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family Gene:YOR121C(YOR121C_d)|FD-Score:-3.35|P-value:4.07E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W Gene:YPR098C(YPR098C)|FD-Score:-3.19|P-value:7.00E-4||SGD DESC:Protein of unknown function, localized to the mitochondrial outer membrane

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YDL102W4.798.14E-70.76POL3Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
YDL016C_d4.201.35E-50.76YDL016C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CDC7/YDL153C, the catalytic subunit of a complex that regulates DNA replication
YGR029W3.442.92E-40.24ERV1Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
YLR321C3.206.89E-40.17SFH1Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog
YBR247C3.030.001240.02ENP1Protein associated with U3 and U14 snoRNAs, required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus
YLR197W3.010.001320.02NOP56Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
YKR062W2.990.001400.08TFA2TFIIE small subunit, involved in RNA polymerase II transcription initiation
YKR071C2.910.001800.05DRE2Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
YDL166C2.860.002110.04FAP7Essential NTPase required for small ribosome subunit synthesis, mediates processing of the 20S pre-rRNA at site D in the cytoplasm but associates only transiently with 43S preribosomes via Rps14p, may be the endonuclease for site D
YDR081C2.820.002370.07PDC2Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions
YOR146W_d2.750.002970.01YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YDR412W2.750.003020.04RRP17Component of the pre-60S pre-ribosomal particle; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; exonuclease required for 5′ end processing of pre-60S ribosomal RNA
YPR112C2.710.003370.06MRD1Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
YDR188W2.650.003980.04CCT6Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
YLR230W_d2.610.004500.05YLR230W_dDubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YDL168W4.161.57E-5SFA1Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
YDR198C3.993.37E-5RKM2Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
YCL002C_p3.875.48E-5YCL002C_pPutative protein of unknown function; YCL002C is not an essential gene
YGR081C3.865.60E-5SLX9Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
YPL179W3.836.28E-5PPQ1Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors
YGL014W3.611.51E-4PUF4Member of the PUF protein family, which is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
YER121W_p3.542.01E-4YER121W_pPutative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy
YGR204W3.522.18E-4ADE3Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
YBR246W3.462.67E-4RRT2Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
YPR122W3.452.82E-4AXL1Haploid specific endoprotease that performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
YDL117W3.452.85E-4CYK3SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
YJL205C3.442.96E-4NCE101Protein of unknown function, involved in secretion of proteins that lack classical secretory signal sequences
YDR049W3.383.57E-4VMS1Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
YPR118W3.373.76E-4MRI15'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
YMR275C3.353.99E-4BUL1Ubiquitin-binding component of the Rsp5p E3-ubiquitin ligase complex; disruption causes temperature-sensitive growth, overexpression causes missorting of amino acid permeases; BUL1 has a paralog, BUL2, that arose from the whole genome duplication

GO enrichment analysis for SGTC_2388
No biological processes are significantly enriched (FDR < 0.1).

No protein complexes are significantly enriched (FDR < 0.1).

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.1254.66E-22SGTC_23839036125 200.0 μMChembridge (Fragment library)795800.0847458
0.0952.50E-13SGTC_32449134489 49.5 μMChembridge (Drug-like library)165842710.084507
0.0944.23E-13SGTC_1230083-0120 66.7 μMChemDiv (Drug-like library)160017010.111111
0.0809.10E-10SGTC_23869075165 157.4 μMChembridge (Fragment library)164522770.192308
0.0655.80E-7SGTC_1822st052468 42.0 μMTimTec (Natural product derivative library)28736780.104651
0.0588.46E-6SGTC_1121fusaric acid 630.5 nMTimTec (Natural product library)34420.134615
0.0552.28E-5SGTC_24425158906 48.5 μMMiscellaneous18938350.0793651
0.0527.34E-5SGTC_14770139-0234 16.0 μMChemDiv (Drug-like library)6208800.0545455
0.0518.15E-5SGTC_30419091929 49.5 μMChembridge (Drug-like library)252368870.112903redox potentiating
0.0519.44E-5SGTC_23859074692 200.0 μMChembridge (Fragment library)170469060.0851064
0.0491.93E-4SGTC_24485185112 27.2 μMChembridge (Fragment library)57181890.0847458
0.0472.82E-4SGTC_26373-alpha-acetoxydihydrodeoxygedunin 100.0 μMMicrosource (Natural product library)67085140.0853659
0.0464.22E-4SGTC_2735sertaconazole 140.0 nMMiscellaneous658630.0506329plasma membrane duress
0.0464.29E-4SGTC_20875276107 200.0 μMChembridge (Fragment library)1444050.111111
0.0454.99E-4SGTC_15153460-0031 42.1 μMChemDiv (Drug-like library)28765960.142857DNA intercalators

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_9383126-1755342 μM0.3829791777771ChemDiv (Drug-like library)390.388723.01235
SGTC_9861493-048384 μM0.36956540079ChemDiv (Drug-like library)256.256681.31914
SGTC_9272425-1185123 μM0.363636730375ChemDiv (Drug-like library)316.353243.13522
SGTC_1759st04305970 μM0.346939669426TimTec (Natural product derivative library)285.29462.45314amide catabolism
SGTC_2923799265458.14 μM0.342985126Chembridge (Drug-like library)358.432983.9612Golgi
SGTC_2864903475547.6 μM0.3333334930451Chembridge (Drug-like library)361.393862.58124
SGTC_8500388-014375.9 μM0.31914967273ChemDiv (Drug-like library)277.31723.76722
SGTC_2794778538227.27 μM0.3090912205081Chembridge (Drug-like library)396.45953.57225
SGTC_3108912178549.47 μM0.30909117140271Chembridge (Drug-like library)312.3632.79223
SGTC_2885905773251.95 μM0.3018876471414Chembridge (Drug-like library)297.414564.71512