5hr heat shock (37°C) + mitomycin C FDA approved compound

Mitomycin is a bifunctional and trifunctional alkylating agent, cross-links DNA inhibiting DNA synthesis and function. Mitomycin is cell cycle phase-nonspecific.

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_239
Screen concentration 819.4 μM
Source Miscellaneous
PubChem CID 5746
SMILES CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N
Standardized SMILES COC12C3NC3CN1C4=C(C2COC(=O)N)C(=O)C(=C(C)C4=O)N
Molecular weight 334.3272
ALogP -1.55
H-bond donor count 3
H-bond acceptor count 8
Response signature heat shock/prefoldin

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.6
% growth inhibition (Hom. pool) 2.87


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 5746
Download HIP data (tab-delimited text)  (excel)
Gene:AFG2(YLR397C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.16||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC48(YDL126C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:ILS1(YBL076C)|FD-Score:-3.37|P-value:3.80E-4|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MTR2(YKL186C)|FD-Score:-3.8|P-value:7.37E-5|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:PGI1(YBR196C)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:SEC1(YDR164C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.17||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC13(YLR208W)|FD-Score:5.96|P-value:1.24E-9|Clearance:1.36||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:3.73|P-value:9.54E-5|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:4.61|P-value:2.06E-6|Clearance:0.87||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase Gene:AFG2(YLR397C)|FD-Score:3.39|P-value:3.45E-4|Clearance:0.16||SGD DESC:ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs Gene:CDC48(YDL126C)|FD-Score:-4.08|P-value:2.29E-5|Clearance:0||SGD DESC:AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP Gene:ILS1(YBL076C)|FD-Score:-3.37|P-value:3.80E-4|Clearance:0||SGD DESC:Cytoplasmic isoleucine-tRNA synthetase, target of the G1-specific inhibitor reveromycin A Gene:MTR2(YKL186C)|FD-Score:-3.8|P-value:7.37E-5|Clearance:0||SGD DESC:mRNA transport regulator, essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA Gene:PGI1(YBR196C)|FD-Score:-3.57|P-value:1.79E-4|Clearance:0||SGD DESC:Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation Gene:SEC1(YDR164C)|FD-Score:3.23|P-value:6.20E-4|Clearance:0.17||SGD DESC:Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function Gene:SEC13(YLR208W)|FD-Score:5.96|P-value:1.24E-9|Clearance:1.36||SGD DESC:Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress Gene:YGR114C(YGR114C_d)|FD-Score:3.73|P-value:9.54E-5|Clearance:0.34||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6 Gene:YGR115C(YGR115C_d)|FD-Score:4.61|P-value:2.06E-6|Clearance:0.87||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C Gene:YMR290W-A(YMR290W-A_d)|FD-Score:-3.49|P-value:2.42E-4|Clearance:0||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential HAS1 gene which encodes an ATP-dependent RNA helicase

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 5746
Download HOP data (tab-delimited text)  (excel)
Gene:AAT2(YLR027C)|FD-Score:16.6|P-value:2.60E-62||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AFT1(YGL071W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARD1(YHR013C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP5(YNL059C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BCK1(YJL095W)|FD-Score:8.5|P-value:9.67E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIM1(YER016W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:-3.75|P-value:8.99E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:BUD28(YLR062C_d)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD31(YCR063W)|FD-Score:7.87|P-value:1.76E-15||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAF130(YGR134W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAP1(YKL007W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBF2(YGR092W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBP7(YKR024C)|FD-Score:5.9|P-value:1.87E-9||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DGR1(YNL130C-A_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOA4(YDR069C)|FD-Score:6.46|P-value:5.11E-11||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FRE3(YOR381W)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUR4(YBR021W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV5(YCL058C)|FD-Score:-4.01|P-value:3.03E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GCR2(YNL199C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GET2(YER083C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIM5(YML094W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLE2(YER107C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HIS2(YFR025C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOT13(YKL084W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HUL5(YGL141W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IRC15(YPL017C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:MAC1(YMR021C)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:6.59|P-value:2.17E-11||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MOG1(YJR074W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL25(YGR076C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:NTC20(YBR188C)|FD-Score:7.17|P-value:3.82E-13||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NUP120(YKL057C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OSW1(YOR255W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAT1(YCR077C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDC1(YLR044C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PKH3(YDR466W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSO2(YMR137C)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD10(YML095C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAS2(YNL098C)|FD-Score:5.66|P-value:7.65E-9||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPE1(YJL121C)|FD-Score:4.78|P-value:8.70E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPS10B(YMR230W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRP6(YOR001W)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:RSA3(YLR221C)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RVS161(YCR009C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SEC66(YBR171W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLT2(YHR030C)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPC72(YAL047C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPP1(YPL138C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SRB2(YHR041C)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO9(YCL037C)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SWM1(YDR260C)|FD-Score:12.2|P-value:1.08E-34||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TOM1(YDR457W)|FD-Score:9.34|P-value:4.77E-21||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM70(YNL121C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM7(YBR061C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSC3(YBR058C-A)|FD-Score:17|P-value:1.81E-65||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:URA7(YBL039C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA13(YPR036W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VOA1(YGR106C)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS45(YGL095C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR013C(YBR013C_p)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCR064C(YCR064C_d)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR134C(YDR134C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:9.98|P-value:9.40E-24||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YML094C-A(YML094C-A_d)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR084W(YMR084W_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL140C(YNL140C_p)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL170W(YNL170W_d)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL036W(YOL036W_p)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins Gene:AAT2(YLR027C)|FD-Score:16.6|P-value:2.60E-62||SGD DESC:Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells Gene:AFT1(YGL071W)|FD-Score:3.32|P-value:4.53E-4||SGD DESC:Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress Gene:ARD1(YHR013C)|FD-Score:3.11|P-value:9.33E-4||SGD DESC:Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; acetylates many proteins and thus affects telomeric silencing, cell cycle, heat-shock resistance, mating, and sporulation; human Ard1p levels are elevated in cancer cells; protein abundance increases in response to DNA replication stress Gene:ARO1(YDR127W)|FD-Score:5.2|P-value:1.02E-7||SGD DESC:Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids Gene:ARO7(YPR060C)|FD-Score:3.54|P-value:2.02E-4||SGD DESC:Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis Gene:ARP5(YNL059C)|FD-Score:4.46|P-value:4.08E-6||SGD DESC:Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes Gene:BCK1(YJL095W)|FD-Score:8.5|P-value:9.67E-18||SGD DESC:Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p Gene:BIM1(YER016W)|FD-Score:3.11|P-value:9.27E-4||SGD DESC:Microtubule-binding protein that together with Kar9p makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally Gene:BTS1(YPL069C)|FD-Score:3.23|P-value:6.29E-4||SGD DESC:Geranylgeranyl diphosphate synthase, increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic Gene:BUD23(YCR047C)|FD-Score:-3.75|P-value:8.99E-5||SGD DESC:Methyltransferase, methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; diploid mutant displays random budding pattern Gene:BUD28(YLR062C_d)|FD-Score:-3.1|P-value:9.57E-4||SGD DESC:Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay Gene:BUD31(YCR063W)|FD-Score:7.87|P-value:1.76E-15||SGD DESC:Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis Gene:CAF130(YGR134W)|FD-Score:3.56|P-value:1.89E-4||SGD DESC:Part of the evolutionarily-conserved CCR4-NOT transcriptional regulatory complex involved in controlling mRNA initiation, elongation, and degradation Gene:CAP1(YKL007W)|FD-Score:4.24|P-value:1.14E-5||SGD DESC:Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress Gene:CCW12(YLR110C)|FD-Score:3.59|P-value:1.62E-4||SGD DESC:Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication Gene:COG7(YGL005C)|FD-Score:3.47|P-value:2.64E-4||SGD DESC:Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments Gene:DBF2(YGR092W)|FD-Score:3.67|P-value:1.22E-4||SGD DESC:Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis Gene:DBP7(YKR024C)|FD-Score:5.9|P-value:1.87E-9||SGD DESC:Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions Gene:DGR1(YNL130C-A_p)|FD-Score:4.22|P-value:1.22E-5||SGD DESC:Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose Gene:DOA4(YDR069C)|FD-Score:6.46|P-value:5.11E-11||SGD DESC:Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole Gene:FRE3(YOR381W)|FD-Score:-4.59|P-value:2.22E-6||SGD DESC:Ferric reductase, reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels Gene:FUR4(YBR021W)|FD-Score:3.11|P-value:9.51E-4||SGD DESC:Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation Gene:FYV5(YCL058C)|FD-Score:-4.01|P-value:3.03E-5||SGD DESC:Protein involved in regulation of the mating pathway; binds with Matalpha2p to promoters of haploid-specific genes; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis Gene:GCR2(YNL199C)|FD-Score:3.71|P-value:1.03E-4||SGD DESC:Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p Gene:GET2(YER083C)|FD-Score:3.25|P-value:5.74E-4||SGD DESC:Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division Gene:GIM5(YML094W)|FD-Score:3.16|P-value:7.78E-4||SGD DESC:Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it Gene:GLE2(YER107C)|FD-Score:5.07|P-value:1.99E-7||SGD DESC:RNA export factor associated with the nuclear pore complex (NPC); associates with NUP116p; required for polyadenylated RNA export but not for protein import; homologous to S. pombe Rae1p and human RAE1 Gene:HIS2(YFR025C)|FD-Score:-3.13|P-value:8.60E-4||SGD DESC:Histidinolphosphatase, catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control Gene:HOT13(YKL084W)|FD-Score:3.9|P-value:4.87E-5||SGD DESC:Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc Gene:HUL5(YGL141W)|FD-Score:3.17|P-value:7.55E-4||SGD DESC:Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD Gene:IRC15(YPL017C)|FD-Score:4.64|P-value:1.75E-6||SGD DESC:Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci Gene:MAC1(YMR021C)|FD-Score:4.6|P-value:2.07E-6||SGD DESC:Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport Gene:MCM21(YDR318W)|FD-Score:6.59|P-value:2.17E-11||SGD DESC:Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2 Gene:MOG1(YJR074W)|FD-Score:3.35|P-value:4.09E-4||SGD DESC:Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p Gene:MRPL25(YGR076C)|FD-Score:3.88|P-value:5.18E-5||SGD DESC:Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan Gene:NTC20(YBR188C)|FD-Score:7.17|P-value:3.82E-13||SGD DESC:Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs Gene:NUP120(YKL057C)|FD-Score:3.2|P-value:6.94E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160 Gene:NUP133(YKR082W)|FD-Score:3.18|P-value:7.39E-4||SGD DESC:Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP133 Gene:OSW1(YOR255W)|FD-Score:3.12|P-value:9.12E-4||SGD DESC:Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p Gene:PAT1(YCR077C)|FD-Score:3.38|P-value:3.63E-4||SGD DESC:Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress Gene:PDC1(YLR044C)|FD-Score:3.94|P-value:4.07E-5||SGD DESC:Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde; subject to glucose-, ethanol-, and autoregulation; involved in amino acid catabolism Gene:PKH3(YDR466W)|FD-Score:3.32|P-value:4.56E-4||SGD DESC:Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant Gene:PSO2(YMR137C)|FD-Score:4.33|P-value:7.52E-6||SGD DESC:Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress Gene:RAD10(YML095C)|FD-Score:3.09|P-value:9.87E-4||SGD DESC:Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein Gene:RAS2(YNL098C)|FD-Score:5.66|P-value:7.65E-9||SGD DESC:GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes Gene:RPE1(YJL121C)|FD-Score:4.78|P-value:8.70E-7||SGD DESC:D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress Gene:RPS10B(YMR230W)|FD-Score:-3.4|P-value:3.39E-4||SGD DESC:Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication Gene:RRP6(YOR001W)|FD-Score:4.96|P-value:3.46E-7||SGD DESC:Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay Gene:RSA3(YLR221C)|FD-Score:5.9|P-value:1.79E-9||SGD DESC:Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus Gene:RVS161(YCR009C)|FD-Score:3.97|P-value:3.62E-5||SGD DESC:Amphiphysin-like lipid raft protein; interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress Gene:SEC66(YBR171W)|FD-Score:4.16|P-value:1.59E-5||SGD DESC:Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER Gene:SLT2(YHR030C)|FD-Score:5.4|P-value:3.31E-8||SGD DESC:Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway Gene:SPC72(YAL047C)|FD-Score:3.18|P-value:7.38E-4||SGD DESC:Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them to microtubules; has roles in astral microtubule formation and stabilization Gene:SPP1(YPL138C)|FD-Score:3.58|P-value:1.72E-4||SGD DESC:Subunit of COMPASS (Set1C), a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein Gene:SRB2(YHR041C)|FD-Score:5.11|P-value:1.62E-7||SGD DESC:Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance Gene:SRO9(YCL037C)|FD-Score:-3.33|P-value:4.30E-4||SGD DESC:Cytoplasmic RNA-binding protein; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; SRO9 has a paralog, SLF1, that arose from the whole genome duplication Gene:SWM1(YDR260C)|FD-Score:12.2|P-value:1.08E-34||SGD DESC:Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation Gene:TOM1(YDR457W)|FD-Score:9.34|P-value:4.77E-21||SGD DESC:E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase Gene:TOM70(YNL121C)|FD-Score:3.63|P-value:1.44E-4||SGD DESC:Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins; TOM70 has a paralog, TOM71, that arose from the whole genome duplication Gene:TRM7(YBR061C)|FD-Score:3.72|P-value:1.00E-4||SGD DESC:2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively Gene:TSC3(YBR058C-A)|FD-Score:17|P-value:1.81E-65||SGD DESC:Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis Gene:UBR1(YGR184C)|FD-Score:3.73|P-value:9.60E-5||SGD DESC:E3 ubiquitin ligase (N-recognin); forms heterodimer with Rad6p to ubiquitinate substrates in the N-end rule pathway; regulates peptide transport via Cup9p ubiquitination; plays a major role in targeting cytosolic misfolded proteins for degradation; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS) Gene:URA7(YBL039C)|FD-Score:-3.47|P-value:2.64E-4||SGD DESC:Major CTP synthase isozyme (see also URA8); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has a paralog, URA8, that arose from the whole genome duplication Gene:UTR1(YJR049C)|FD-Score:-3.26|P-value:5.50E-4||SGD DESC:ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication Gene:VMA13(YPR036W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase Gene:VOA1(YGR106C)|FD-Score:4.45|P-value:4.32E-6||SGD DESC:Endoplasmic reticulum protein that functions, together with other assembly factors, in assembly of the V0 sector of the vacuolar ATPase (V-ATPase); null mutation enhances the V-ATPase deficiency of a vma21 mutant impaired in ER retrieval Gene:VPS45(YGL095C)|FD-Score:3.24|P-value:6.02E-4||SGD DESC:Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment Gene:VPS71(YML041C)|FD-Score:-3.2|P-value:6.80E-4||SGD DESC:Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting Gene:YBR013C(YBR013C_p)|FD-Score:5.46|P-value:2.35E-8||SGD DESC:Putative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein Gene:YCR064C(YCR064C_d)|FD-Score:4.33|P-value:7.43E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31 Gene:YDR134C(YDR134C)|FD-Score:-3.12|P-value:9.06E-4||SGD DESC:Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication Gene:YER091C-A(YER091C-A_d)|FD-Score:-3.3|P-value:4.78E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YJR020W(YJR020W_d)|FD-Score:3.98|P-value:3.50E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YKE2(YLR200W)|FD-Score:9.98|P-value:9.40E-24||SGD DESC:Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin Gene:YML094C-A(YML094C-A_d)|FD-Score:4.73|P-value:1.15E-6||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO Gene:YMR084W(YMR084W_p)|FD-Score:3.27|P-value:5.39E-4||SGD DESC:Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1 Gene:YNL140C(YNL140C_p)|FD-Score:4.61|P-value:1.97E-6||SGD DESC:Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C Gene:YNL170W(YNL170W_d)|FD-Score:3.83|P-value:6.41E-5||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOL036W(YOL036W_p)|FD-Score:4.96|P-value:3.54E-7||SGD DESC:Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication Gene:YPK1(YKL126W)|FD-Score:3.51|P-value:2.27E-4||SGD DESC:Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication Gene:YTP1(YNL237W)|FD-Score:4.29|P-value:8.90E-6||SGD DESC:Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins

Click on Significant Values for Strain Details

Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YLR208W5.961.24E-91.36SEC13Structural component of 3 distinct complexes; subunit of Nup84 nuclear pore sub-complex (NPC), COPII vesicle coat, and Seh1-associated (SEA) complex; COPII vesicle coat is required for ER to Golgi transport; the Nup84 subcomplex contributes to nucleocytoplasmic transport, NPC biogenesis and processes that may require localization of chromosomes at the nuclear periphery, including transcription; homologous to human SEC13; abundance increases under DNA replication stress
YGR115C_d4.612.06E-60.88YGR115C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified and essential ORF SPT6/YGR115C
YGR114C_d3.739.54E-50.34YGR114C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
YLR397C3.393.45E-40.16AFG2ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
YDR164C3.236.20E-40.17SEC1Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function
YDR228C3.060.001120.04PCF11mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
YNL312W3.010.001290.06RFA2Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; protein abundance increases in response to DNA replication stress
YLR115W2.950.001580.01CFT2Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
YGR195W2.940.001620.01SKI6Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
YER165W2.940.001660.01PAB1Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
YOR146W_d2.930.001700.01YOR146W_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene PNO1/YOR145C
YMR076C2.920.001740.02PDS5Protein required for establishment and maintenance of sister chromatid condensation and cohesion, colocalizes with cohesin on chromosomes, may function as a protein-protein interaction scaffold; also required during meiosis
YDR302W2.900.001890.04GPI11ER membrane protein involved in a late step of glycosylphosphatidylinositol (GPI) anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated GPI intermediate; human PIG-Fp is a functional homolog
YNR038W2.850.002170.01DBP6Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family
YML049C2.840.002260.02RSE1Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YBR058C-A17.001.81E-65TSC3Protein that stimulates the activity of serine palmitoyltransferase (Lcb1p, Lcb2p) several-fold; involved in sphingolipid biosynthesis
YLR027C16.602.60E-62AAT2Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
YDR260C12.201.08E-34SWM1Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
YLR200W9.989.40E-24YKE2Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin
YDR457W9.344.77E-21TOM1E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
YJL095W8.509.67E-18BCK1Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p
YCR063W7.871.76E-15BUD31Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
YBR188C7.173.82E-13NTC20Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
YDR318W6.592.17E-11MCM21Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
YDR069C6.465.11E-11DOA4Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
YLR221C5.901.79E-9RSA3Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
YKR024C5.901.87E-9DBP7Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
YNL098C5.667.65E-9RAS2GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
YBR013C_p5.462.35E-8YBR013C_pPutative protein of unknown function, haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein
YHR030C5.403.31E-8SLT2Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway

GO enrichment analysis for SGTC_239
biological processes

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.6260SGTC_2405hr heat shock (37°C) + Streptovitacin 300.0 μMMiscellaneous914670.1125heat shock/prefoldin
0.6150SGTC_2435hr heat shock (37°C) + splitomicin 1.0 μMMiscellaneous52690.0533333heat shock/prefoldin
0.5300SGTC_2385hr heat shock (37°C) + nsc-638432 8.9 μMMiscellaneous3680900.0543478heat shock/prefoldin
0.4940SGTC_2425hr heat shock (37°C) + 5-fluorouracil 4.6 μMMiscellaneous33850.0606061heat shock/prefoldin
0.4910SGTC_2415hr heat shock (37°C) + cantharidin 155.4 μMICCB bioactive library25450.107692cell wall signaling
0.3154.06E-135SGTC_520329-0193 875.0 nMChemDiv (Drug-like library)37392920.0740741TSC3-RPN4
0.3042.53E-126SGTC_32369132701 49.5 μMChembridge (Drug-like library)242825240.1TSC3-RPN4
0.2921.12E-115SGTC_21105483698 200.0 μMChembridge (Fragment library)28487650.0649351TSC3-RPN4
0.2827.84E-108SGTC_18584-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 62.6 μMTimTec (Natural product derivative library)7563770.0574713TSC3-RPN4
0.2671.49E-96SGTC_1604st002052 72.4 μMTimTec (Natural product derivative library)62548340.05TSC3-RPN4
0.2621.47E-92SGTC_8850865-0106 16.8 μMChemDiv (Drug-like library)27933800.0526316TSC3-RPN4
0.2591.85E-90SGTC_9851493-0307 8.7 μMChemDiv (Drug-like library)32782740.0512821TSC3-RPN4
0.2571.57E-89SGTC_12991079-1439 3.7 μMChemDiv (Drug-like library)3231610.0465116TSC3-RPN4
0.2464.07E-82SGTC_1603st002045 67.8 μMTimTec (Natural product derivative library)58879850.05TSC3-RPN4
0.2452.69E-81SGTC_7901575-0012 79.1 μMChemDiv (Drug-like library)3787410.108108TSC3-RPN4

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_2677mitomycin C100 μM15746Miscellaneous334.32722-1.54738DNA damage response
SGTC_420mitomycin C81.9 mM15746Miscellaneous334.32722-1.54738
SGTC_1639Lupinine o-methylbenzoicacid ester hydrochloride61.8 μM0.162517037758TimTec (Natural product derivative library)323.857544.19413ergosterol biosynthesis
SGTC_1660st01296028.1 μM0.162524207266TimTec (Natural product derivative library)580.4471550.052815iron homeostasis
SGTC_22907904846200 μM0.1621622962154Chembridge (Fragment library)280.243612.17216
SGTC_2712st07912647.7 μM0.1590913152970TimTec (Natural product derivative library)419.426642.51617
SGTC_2894904862532.47 μM0.1558444092861Chembridge (Drug-like library)278.346781.38214
SGTC_1877wortmannin25 μM0.153061312145ICCB bioactive library428.431861.70508
SGTC_2422chlorphenesin carbamate384.24 μM0.1527782724Miscellaneous245.659581.59724
SGTC_2946906550271.43 μM0.15116316489101Chembridge (Drug-like library)324.37372.35613
SGTC_466geldanamycin89.2 μM0.1509435288382ICCB bioactive library560.63592.3639RPP1 & pyrimidine depletion