6285546

N-(5-chloro-2-methylphenyl)-2-cyanoacetamide

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Source SMILES: standardizedunstandardized
Compound:
Screen ID SGTC_2391
Screen concentration 200.0 μM
Source Chembridge (Fragment library)
PubChem CID 846083
SMILES CC1=C(C=C(C=C1)Cl)NC(=O)CC#N
Standardized SMILES Cc1ccc(Cl)cc1NC(=O)CC#N
Molecular weight 208.6443
ALogP 2.16
H-bond donor count 1
H-bond acceptor count 2
Response signature

Pool Growth Kinetics
% growth inhibition (Het. pool) 9.61
% growth inhibition (Hom. pool) 5.84


HIP profile

Heterozygous deletion strains
Scale: fixedexpanded
Heterozygous profile for 846083
Download HIP data (tab-delimited text)  (excel)
Gene:ALG14(YBR070C)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CCT2(YIL142W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.03||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:5.42|P-value:3.00E-8|Clearance:0.84||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.02||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:IRA1(YBR140C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NOC2(YOR206W)|FD-Score:3.73|P-value:9.76E-5|Clearance:0.05||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:RPA43(YOR340C)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPL5(YPL131W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:3.91|P-value:4.55E-5|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.09||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.18||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP43(YCR035C)|FD-Score:3.46|P-value:2.67E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:THG1(YGR024C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF35(YDR429C)|FD-Score:-4.17|P-value:1.51E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.05||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:4.58|P-value:2.30E-6|Clearance:0.67||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YLR076C(YLR076C_d)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YTH1(YPR107C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation Gene:ALG14(YBR070C)|FD-Score:-3.1|P-value:9.62E-4|Clearance:0||SGD DESC:Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases Gene:CCT2(YIL142W)|FD-Score:3.46|P-value:2.68E-4|Clearance:0.03||SGD DESC:Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT3(YJL014W)|FD-Score:5.42|P-value:3.00E-8|Clearance:0.84||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CCT7(YJL111W)|FD-Score:3.83|P-value:6.54E-5|Clearance:0.1||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance Gene:CCT8(YJL008C)|FD-Score:3.51|P-value:2.26E-4|Clearance:0.02||SGD DESC:Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Gene:CSE1(YGL238W)|FD-Score:3.48|P-value:2.47E-4|Clearance:0.02||SGD DESC:Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation Gene:IRA1(YBR140C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0||SGD DESC:GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication Gene:NOC2(YOR206W)|FD-Score:3.73|P-value:9.76E-5|Clearance:0.05||SGD DESC:Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors Gene:RPA43(YOR340C)|FD-Score:3.87|P-value:5.49E-5|Clearance:0.04||SGD DESC:RNA polymerase I subunit A43 Gene:RPL5(YPL131W)|FD-Score:3.68|P-value:1.18E-4|Clearance:0.03||SGD DESC:Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly Gene:RPP1(YHR062C)|FD-Score:3.91|P-value:4.55E-5|Clearance:0.05||SGD DESC:Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs Gene:RPS2(YGL123W)|FD-Score:3.6|P-value:1.60E-4|Clearance:0.09||SGD DESC:Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5 Gene:RPS3(YNL178W)|FD-Score:3.24|P-value:6.06E-4|Clearance:0.18||SGD DESC:Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease activity; essential for viability; homologous to mammalian ribosomal protein S3 and bacterial S3 Gene:RRP43(YCR035C)|FD-Score:3.46|P-value:2.67E-4|Clearance:0||SGD DESC:Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress Gene:THG1(YGR024C)|FD-Score:3.44|P-value:2.95E-4|Clearance:0.06||SGD DESC:tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes Gene:TIF35(YDR429C)|FD-Score:-4.17|P-value:1.51E-5|Clearance:0||SGD DESC:eIF3g subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation Gene:UBC1(YDR177W)|FD-Score:3.65|P-value:1.33E-4|Clearance:0.05||SGD DESC:Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress Gene:YDR187C(YDR187C_d)|FD-Score:4.58|P-value:2.30E-6|Clearance:0.67||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W Gene:YLR076C(YLR076C_d)|FD-Score:3.38|P-value:3.64E-4|Clearance:0.14||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10 Gene:YTH1(YPR107C)|FD-Score:-4.14|P-value:1.74E-5|Clearance:0||SGD DESC:Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation

HOP profile

Homozygous deletion strains
Scale: fixedexpanded
Homozygous profile for 846083
Download HOP data (tab-delimited text)  (excel)
Gene:AAD3(YCR107W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFG3(YER017C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:BFR1(YOR198C)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA3(YJL060W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BST1(YFL025C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAF20(YOR276W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:DCG1(YIR030C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:EFG1(YGR271C-A)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EGD2(YHR193C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ENV10(YLR065C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FAR11(YNL127W)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDH1(YOR375C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GEP3(YOR205C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GOT1(YMR292W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GRE3(YHR104W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HPC2(YBR215W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:MAD2(YJL030W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MDM12(YOL009C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MIG2(YGL209W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MND2(YIR025W)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN11(YJL183W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP21(YBL090W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL40(YPL173W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS9(YBR146W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUP3(YHL036W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM2(YLR382C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPI9(YLR338W_d)|FD-Score:6.06|P-value:6.85E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OPY1(YBR129C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PAN5(YHR063C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PET127(YOR017W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHM8(YER037W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PHO89(YBR296C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PSY2(YNL201C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PXA2(YKL188C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RBG1(YAL036C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RER1(YCL001W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RIT1(YMR283C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL12B(YDR418W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTT103(YDR289C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC3(YDR159W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAP30(YMR263W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SKY1(YMR216C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF2(YOR290C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SUR4(YLR372W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:UPS1(YLR193C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VID28(YIL017C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS64(YDR200C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL012C(YBL012C_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR141C(YBR141C_p)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER046W-A(YER046W-A_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHM2(YMR241W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YJL055W(YJL055W_p)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL147C(YJL147C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YJL215C(YJL215C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR149C(YLR149C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR312C(YLR312C_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function Gene:YNL205C(YNL205C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR161C-C(YOR161C-C_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR263C(YOR263C_d)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W Gene:AAD3(YCR107W)|FD-Score:3.65|P-value:1.33E-4||SGD DESC:Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role Gene:AFG3(YER017C)|FD-Score:3.34|P-value:4.13E-4||SGD DESC:Component, with Yta12p, of the mitochondrial inner membrane m-AAA protease; mediates degradation of misfolded or unassembled proteins and is also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging Gene:BFR1(YOR198C)|FD-Score:4.76|P-value:9.48E-7||SGD DESC:Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity Gene:BNA3(YJL060W)|FD-Score:-3.93|P-value:4.30E-5||SGD DESC:Kynurenine aminotransferase, catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate Gene:BST1(YFL025C)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules Gene:CAF20(YOR276W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Phosphoprotein of the mRNA cap-binding complex involved in translational control, repressor of cap-dependent translation initiation, competes with eIF4G for binding to eIF4E Gene:DCG1(YIR030C)|FD-Score:3.38|P-value:3.65E-4||SGD DESC:Protein of unknown function, expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain Gene:EFG1(YGR271C-A)|FD-Score:3.34|P-value:4.25E-4||SGD DESC:Essential protein required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus Gene:EGD2(YHR193C)|FD-Score:4.04|P-value:2.70E-5||SGD DESC:Alpha subunit of the heteromeric nascent polypeptide-associated complex (NAC) involved in protein sorting and translocation, associated with cytoplasmic ribosomes Gene:ENV10(YLR065C)|FD-Score:3.44|P-value:2.93E-4||SGD DESC:Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing; YLR065C is not an essential gene Gene:FAR11(YNL127W)|FD-Score:-3.37|P-value:3.73E-4||SGD DESC:Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far10p; has similarity to the N- and C-termini of N. crassa HAM-2 Gene:GDH1(YOR375C)|FD-Score:3.57|P-value:1.76E-4||SGD DESC:NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication Gene:GEP3(YOR205C)|FD-Score:3.98|P-value:3.46E-5||SGD DESC:Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (phb1) Gene:GOT1(YMR292W)|FD-Score:3.18|P-value:7.28E-4||SGD DESC:Homodimeric protein that is packaged into COPII vesicles and cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition Gene:GRE3(YHR104W)|FD-Score:3.29|P-value:5.03E-4||SGD DESC:Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress Gene:GRX6(YDL010W)|FD-Score:3.71|P-value:1.05E-4||SGD DESC:Cis-golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication Gene:HPC2(YBR215W)|FD-Score:3.7|P-value:1.06E-4||SGD DESC:Subunit of the HIR complex, a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes Gene:MAD2(YJL030W)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I Gene:MDM12(YOL009C)|FD-Score:3.2|P-value:6.79E-4||SGD DESC:Mitochondrial outer membrane protein, required for transmission of mitochondria to daughter cells; component of the ERMES complex that links the ER to mitochondria; may influence import and assembly of outer membrane beta-barrel proteins Gene:MIG2(YGL209W)|FD-Score:3.11|P-value:9.35E-4||SGD DESC:Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced repression of many genes; under low glucose conditions Mig2p relocalizes to mitochondrion, where it interacts with Ups1p and antagonizes mitochondrial fission factor, Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth along with Mig2p in response to glucose depletion; MIG2 has a paralog, MIG3, that arose from the whole genome duplication Gene:MMM1(YLL006W)|FD-Score:3.76|P-value:8.57E-5||SGD DESC:ER integral membrane protein, component of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth Gene:MND2(YIR025W)|FD-Score:-3.15|P-value:8.14E-4||SGD DESC:Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase Gene:MNN11(YJL183W)|FD-Score:4.16|P-value:1.61E-5||SGD DESC:Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p Gene:MRP21(YBL090W)|FD-Score:4.16|P-value:1.57E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences Gene:MRPL40(YPL173W)|FD-Score:3.1|P-value:9.72E-4||SGD DESC:Mitochondrial ribosomal protein of the large subunit Gene:MRPS9(YBR146W)|FD-Score:3.91|P-value:4.56E-5||SGD DESC:Mitochondrial ribosomal protein of the small subunit Gene:MUP3(YHL036W)|FD-Score:3.93|P-value:4.23E-5||SGD DESC:Low affinity methionine permease, similar to Mup1p Gene:NAM2(YLR382C)|FD-Score:3.23|P-value:6.25E-4||SGD DESC:Mitochondrial leucyl-tRNA synthetase, also has a direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance Gene:OPI9(YLR338W_d)|FD-Score:6.06|P-value:6.85E-10||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C Gene:OPY1(YBR129C)|FD-Score:4.99|P-value:3.06E-7||SGD DESC:Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies Gene:PAN5(YHR063C)|FD-Score:-3.5|P-value:2.37E-4||SGD DESC:2-dehydropantoate 2-reductase, part of the pantothenic acid pathway, structurally homologous to E. coli panE Gene:PET127(YOR017W)|FD-Score:-3.34|P-value:4.14E-4||SGD DESC:Protein with a role in 5'-end processing of mitochondrial RNAs, located in the mitochondrial membrane Gene:PHM8(YER037W)|FD-Score:-3.31|P-value:4.65E-4||SGD DESC:Lysophosphatidic acid (LPA) phosphatase involved in LPA hydrolysis in response to phosphate starvation; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p Gene:PHO89(YBR296C)|FD-Score:3.31|P-value:4.69E-4||SGD DESC:Na+/Pi cotransporter, active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification Gene:PSY2(YNL201C)|FD-Score:4.63|P-value:1.85E-6||SGD DESC:Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3 Gene:PXA2(YKL188C)|FD-Score:-3.95|P-value:3.84E-5||SGD DESC:Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder Gene:RBG1(YAL036C)|FD-Score:-4.24|P-value:1.14E-5||SGD DESC:Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid Gene:RER1(YCL001W)|FD-Score:4.25|P-value:1.06E-5||SGD DESC:Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER Gene:RIT1(YMR283C)|FD-Score:-3.37|P-value:3.71E-4||SGD DESC:2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA Gene:RPL12B(YDR418W)|FD-Score:3.32|P-value:4.57E-4||SGD DESC:Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication Gene:RPL33B(YOR234C)|FD-Score:3.59|P-value:1.67E-4||SGD DESC:Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication Gene:RRI2(YOL117W)|FD-Score:5.01|P-value:2.74E-7||SGD DESC:Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response Gene:RTT103(YDR289C)|FD-Score:3.18|P-value:7.49E-4||SGD DESC:Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition Gene:SAC3(YDR159W)|FD-Score:3.22|P-value:6.38E-4||SGD DESC:Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP) Gene:SAP30(YMR263W)|FD-Score:4.09|P-value:2.17E-5||SGD DESC:Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance Gene:SKY1(YMR216C)|FD-Score:4.14|P-value:1.72E-5||SGD DESC:SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1 Gene:SNF2(YOR290C)|FD-Score:3.31|P-value:4.66E-4||SGD DESC:Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p Gene:SUR4(YLR372W)|FD-Score:3.52|P-value:2.18E-4||SGD DESC:Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis Gene:UPS1(YLR193C)|FD-Score:3.47|P-value:2.62E-4||SGD DESC:Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI Gene:VID28(YIL017C)|FD-Score:3.31|P-value:4.70E-4||SGD DESC:GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm Gene:VPS64(YDR200C)|FD-Score:4.06|P-value:2.49E-5||SGD DESC:Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance Gene:YBL008W-A(YBL008W-A_p)|FD-Score:3.14|P-value:8.55E-4||SGD DESC:Putative protein of unknown function; identified by fungal homology and RT-PCR Gene:YBL012C(YBL012C_d)|FD-Score:-3.41|P-value:3.20E-4||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YBR141C(YBR141C_p)|FD-Score:4.36|P-value:6.39E-6||SGD DESC:Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene Gene:YCR087C-A(YCR087C-A_p)|FD-Score:3.4|P-value:3.41E-4||SGD DESC:Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene Gene:YEL045C(YEL045C_d)|FD-Score:3.92|P-value:4.44E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress Gene:YER046W-A(YER046W-A_d)|FD-Score:3.2|P-value:6.95E-4||SGD DESC:Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YGR273C(YGR273C_p)|FD-Score:-3.27|P-value:5.40E-4||SGD DESC:Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; YGR273C is not an essential gene Gene:YHM2(YMR241W)|FD-Score:3.28|P-value:5.18E-4||SGD DESC:Carrier protein that exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome Gene:YJL055W(YJL055W_p)|FD-Score:-3.31|P-value:4.74E-4||SGD DESC:Putative protein of unknown function, proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA; overexpression confers resistance to 5-FOA and 5-FU Gene:YJL147C(YJL147C_p)|FD-Score:4.21|P-value:1.27E-5||SGD DESC:Mitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene Gene:YJL215C(YJL215C_d)|FD-Score:4.08|P-value:2.24E-5||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data Gene:YLR149C(YLR149C_p)|FD-Score:3.4|P-value:3.38E-4||SGD DESC:Protein of unknown function; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; YLR149C is not an essential gene; protein abundance increases in response to DNA replication stress Gene:YLR312C(YLR312C_p)|FD-Score:3.13|P-value:8.77E-4||SGD DESC:Putative protein of unknown function Gene:YNL205C(YNL205C_d)|FD-Score:3.22|P-value:6.48E-4||SGD DESC:Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data Gene:YOR161C-C(YOR161C-C_p)|FD-Score:-3.4|P-value:3.35E-4||SGD DESC:Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching Gene:YOR263C(YOR263C_d)|FD-Score:4.98|P-value:3.24E-7||SGD DESC:Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W

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Top 15 HET fitness defect scores (HIP)

Download all HIP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Clearance Gene Gene Description
YJL014W5.423.00E-80.84CCT3Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YDR187C_d4.582.30E-60.67YDR187C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
YHR062C3.914.55E-50.05RPP1Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
YOR340C3.875.49E-50.04RPA43RNA polymerase I subunit A43
YJL111W3.836.54E-50.10CCT7Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo; mutant has increased aneuploidy tolerance
YOR206W3.739.76E-50.05NOC2Protein that forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes, as well as a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors
YPL131W3.681.18E-40.03RPL5Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
YDR177W3.651.33E-40.05UBC1Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress
YGL123W3.601.60E-40.09RPS2Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; homologous to mammalian ribosomal protein S2 and bacterial S5
YJL008C3.512.26E-40.02CCT8Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YGL238W3.482.47E-40.02CSE1Nuclear envelope protein that mediates the nuclear export of importin alpha (Srp1p), homolog of metazoan CAS protein, required for accurate chromosome segregation
YCR035C3.462.67E-48.79E-4RRP43Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
YIL142W3.462.68E-40.03CCT2Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
YBR140C3.442.95E-41.77E-4IRA1GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; IRA1 has a paralog, IRA2, that arose from the whole genome duplication
YGR024C3.442.95E-40.06THG1tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

Top 15 HOM fitness defect scores (HOP)

Download all HOP Data (tab-delimited text)  (excel)
Deletion Strain FD score P-value Gene Gene Description
YLR338W_d6.066.85E-10OPI9_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
YOL117W5.012.74E-7RRI2Subunit of the COP9 signalosome (CSN) complex that cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
YBR129C4.993.06E-7OPY1Protein of unknown function, overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
YOR263C_d4.983.24E-7YOR263C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W
YOR198C4.769.48E-7BFR1Component of mRNP complexes associated with polyribosomes; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
YNL201C4.631.85E-6PSY2Subunit of protein phosphatase PP4 complex; active complex is composed of catalytic subunit Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
YBR141C_p4.366.39E-6YBR141C_pPutative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YBR141C is not an essential gene
YCL001W4.251.06E-5RER1Protein involved in retention of membrane proteins, including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
YJL147C_p4.211.27E-5YJL147C_pMitochondrial protein of unknown function; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage; YJL147W is a non-essential gene
YFL025C4.211.27E-5BST1GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
YBL090W4.161.57E-5MRP21Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
YJL183W4.161.61E-5MNN11Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
YMR216C4.141.72E-5SKY1SR protein kinase (SRPK) involved in regulating proteins involved in mRNA metabolism and cation homeostasis; similar to human SRPK1
YMR263W4.092.17E-5SAP30Subunit of a histone deacetylase complex, along with Rpd3p and Sin3p, that is involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
YJL215C_d4.082.24E-5YJL215C_dDubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data

GO enrichment analysis for SGTC_2391
No biological processes are significantly enriched (FDR < 0.1).

protein complexes

Coinhibitory screens
Download Coinhibition data (tab-delimited text)  (excel)

Correlation pval Screen ID Condition Source PubChem
CID
Tanimoto
Score
Response
Signature
0.0862.98E-11SGTC_21896047310 154.4 μMChembridge (Fragment library)7115350.078125RPP1 & pyrimidine depletion
0.0864.36E-11SGTC_13021159-0335 255.0 μMChemDiv (Drug-like library)6492840.109375
0.0815.25E-10SGTC_32619137475 49.5 μMChembridge (Drug-like library)170276900.0833333RPP1 & pyrimidine depletion
0.0791.10E-9SGTC_21515563267 85.4 μMChembridge (Fragment library)53108240.0677966
0.0773.62E-9SGTC_1875nocodazole 6.0 μMMiscellaneous41220.144928tubulin folding & SWR complex
0.0741.02E-8SGTC_1661st013063 9.7 μMTimTec (Natural product derivative library)7213950.142857
0.0699.46E-8SGTC_14083909-7976 48.8 μMChemDiv (Drug-like library)241523740.0985916Golgi
0.0681.79E-7SGTC_24984'-Methoxychalcone 54.6 μMMicrosource (Natural product library)6418180.125RPP1 & pyrimidine depletion
0.0664.47E-7SGTC_12890976-0073 69.1 μMChemDiv (Drug-like library)33911360.192308
0.0641.03E-6SGTC_1964st076914 61.0 μMTimTec (Natural product derivative library)242086610.2RPP1 & pyrimidine depletion
0.0631.06E-6SGTC_22907904846 200.0 μMChembridge (Fragment library)29621540.0461538
0.0631.20E-6SGTC_265mycophenolic acid 32.9 μMMiscellaneous4465410.0945946RPP1 & pyrimidine depletion
0.0631.40E-6SGTC_120849-0099 9.3 μMChemDiv (Drug-like library)60713880.104478
0.0621.64E-6SGTC_2745rifampicin 24.3 μMMiscellaneous251414280.0578512
0.0621.75E-6SGTC_22887946289 167.3 μMChembridge (Fragment library)12460520.125

Screens with compounds having similar structures
Screen ID Chemical Concentration Tanimoto Score PubChem ID Source Molecular Weight AlogP H-bond
donor
count
H-bond
acceptor
count
Response
Signature
SGTC_22436634756200 μM0.422222885676Chembridge (Fragment library)234.079262.01512
SGTC_2838900268771.43 μM0.3818186457060Chembridge (Drug-like library)357.877063.64713fatty acid desaturase (OLE1)
SGTC_2809796413312.99 μM0.3442621253665Chembridge (Drug-like library)420.332224.4751360S ribosome export
SGTC_22456637752200 μM0.333333681890Chembridge (Fragment library)199.63421.35112
SGTC_23479049783174.81 μM0.3269236467988Chembridge (Fragment library)267.754521.95413
SGTC_14780453-065635.7 μM0.3207555258496ChemDiv (Drug-like library)347.749762.81415
SGTC_1300136-0219337.66 μM0.32234244ChemDiv (Drug-like library)296.148643.77812
SGTC_7003453-2283142 μM0.307692565664ChemDiv (Drug-like library)263.719462.88912amide catabolism
SGTC_7251497-096319 μM0.3076923549977ChemDiv (Drug-like library)356.117783.73215copper-dependent oxidative stress
SGTC_12580671-004357.1 μM0.3076924298767ChemDiv (Drug-like library)296.772522.72124